Agilent Technologies Revident LC/Q-TOF System User Manual page 117

5 Maintenance
Electrospray Ionization (ESI) source
10 Connect the reference nebulizer sample line and sample nebulizer gas tubing.
In the MassHunter Data Acquisition program:
1 Turn on the LC/MS instrument.
2 While you wait for the set points to equilibrate, prepare and install the calibrant
When all set points are equilibrated:
1 In the tune view or context, turn on the calibrant.
2 Purge the calibrant line for 5 minutes.
3 Do a complete autotune. If an autotune is not available, do a checktune. If the
4 If the checktune results are not acceptable, run autotune on a supported
5 When the instrument is ready, load or create the method to use with the ion
6 Change the method settings for the new ion source.
Agilent Revident LC/Q-TOF System User Guide
for the new ion source.
a Rinse a clean calibrant bottle with LC/MS-grade acetonitrile.
b Pour the appropriate ESI Calibrant into the calibrant bottle for your
instrument.
c Install the calibrant bottle into the calibrant delivery system.
tune report does not show good results, tune the instrument. If needed,
change the source to one that supports autotune.
tuning source.
source.
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