Agilent Technologies Revident LC/Q-TOF System User Manual
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Agilent Technologies Revident LC/Q-TOF System User Manual

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Summary of Contents for Agilent Technologies Revident LC/Q-TOF System

  • Page 1 Revident LC/Q-TOF System User Guide...
  • Page 2 Copyright editions. Further, to the maximum extent A CAUTION notice denotes a permitted by applicable law, Agilent © Agilent Technologies, Inc. 2024 hazard. It calls attention to an disclaims all warranties, either express or operating procedure, practice, or implied, with regard to this manual and...
  • Page 3 In This Guide This guide provides information on the Agilent Revident LC/Q-TOF system running MassHunter Data Acquisition 12.0 or higher. Additional Resources User Documentation Data analysis and library management documentation can be found by scanning the code or navigating to https://aglt.co/DALibMgmtDocs.
  • Page 4 To get answers to your questions, join over 10,000 users in the Agilent Community. Review curated support materials organized by platform technology. Ask questions to industry colleagues and collaborators. Get notifications on new videos, documents, tools, and webinars relevant to your work. https://community.agilent.com/ Agilent Revident LC/Q-TOF System User Guide...

  • Page 5: Table Of Contents

    Instrument Status Indicator Lights Instrument Status Indicators in Acquisition Ion Sources Electrospray Ionization (Dual ESI) source Agilent Jet Stream (Dual AJS ESI) source Atmospheric Pressure Chemical Ionization (APCI) source Tuning About Tuning Roles Configure Tune Agilent Revident LC/Q-TOF System User Guide...
  • Page 6 Performing a checktune or autotune Switching LC stream to MS Monitoring MS baseline and spectral displays Select the Q-TOF/TOF ion source Internal Reference Mass Correction Viewing the System Activity Log for events and errors Agilent Revident LC/Q-TOF System User Guide...
  • Page 7 Installing the capillary Removing the capillary Cleaning the capillary Cleaning a clogged capillary Flushing the Calibrant Delivery System (CDS) Replacing the MS selection valve rotor seal Cleaning the corona needle Replacing the corona needle Disinfection Agilent Revident LC/Q-TOF System User Guide...
  • Page 8 Content Source Maintenance Changing to a source that uses a different tuning mix Installing and removing sources Electrospray Ionization (ESI) source Agilent Jet Stream (AJS ESI) source Atmospheric Pressure Chemical Ionization (APCI) source Agilent Revident LC/Q-TOF System User Guide...

  • Page 9: Introduction

    Agilent Regulatory Compliance Statement CE Compliance UK Compliance Electromagnetic Compatibility EMC Declaration for South Korea Detachable Power Cord Declaration for Japan Sound Emission Certification for Federal Republic of Germany Waste Electrical and Electronic Equipment (WEEE) Directive Agilent Revident LC/Q-TOF System User Guide...

  • Page 10: Sustainability Information

    Product End-of-Life Agilent offers a take-back program for its Revident LC/Q-TOF LC/MS system customers in US, EU, and UK markets. See https://www.agilent.com/environment/product/index.shtml for more information. Agilent Revident LC/Q-TOF System User Guide...

  • Page 11: Agilent Regulatory Compliance Statement

    Declaration of Conformity. The Declaration of Conformity is the legal declaration by Agilent that the product complies with the relevant directives listed above, and shows the EN standards to which the product was tested to demonstrate compliance. Agilent Revident LC/Q-TOF System User Guide...

  • Page 12: Uk Compliance

    Declaration of Conformity. The Declaration of Conformity is the legal declaration by Agilent that the product complies with the relevant regulations listed above, and shows the designated standards to which the product was tested to demonstrate compliance. Agilent Revident LC/Q-TOF System User Guide...

  • Page 13: Electromagnetic Compatibility

    4 Make sure that all peripheral devices are also certified. 5 Make sure that appropriate cables are used to connect the device to peripheral equipment. 6 Consult your equipment dealer, Agilent Technologies, or an experienced technician for assistance. Agilent Revident LC/Q-TOF System User Guide...

  • Page 14: Emc Declaration For South Korea

    Introduction EMC Declaration for South Korea Changes or modifications not expressly approved by Agilent Technologies could void the user’s authority to operate the equipment. EMC Declaration for South Korea 사용자안내문 This equipment has been evaluated for its suitability for use in a commercial environment.

  • Page 15: Sound Emission Certification For Federal Republic Of Germany

    The affixed label indicates that you must not discard this electrical/electronic product in domestic household waste. Do not dispose of in domestic household waste. NOTE To return unwanted products, contact your local Agilent office or see https://www.agilent.com/environment/product/index.shtml for more information. Agilent Revident LC/Q-TOF System User Guide...
  • Page 16 This page intentionally left blank. Agilent Revident LC/Q-TOF System User Guide...

  • Page 17: Hardware Overview

    Calibrant Delivery System Side view Back view Instrument Status Indicator Lights Instrument Status Indicators in Acquisition Ion Sources Electrospray Ionization (Dual ESI) source Agilent Jet Stream (Dual AJS ESI) source Atmospheric Pressure Chemical Ionization (APCI) source Agilent Revident LC/Q-TOF System User Guide...

  • Page 18: Revident Lc/Q-Tof

    Hardware Overview Revident LC/Q-TOF Revident LC/Q-TOF Front view Flight Tube CDS Bottles Status Indicator Ion Source Front Cover Service Panel Serial Number Power Switch Figure 1. Front view of Revident LC/Q-TOF Agilent Revident LC/Q-TOF System User Guide...

  • Page 19: Calibrant Delivery System

    Calibrant Delivery System Tune Mix Pump Internal Reference Mass Pump LC Flow Inlet Stop Valves LC/MS Selector Valve Internal Reference Mass Selector Valve Leak Sensor Tune Mix Bottle Reference Solution Figure 2. Calibrant delivery system Agilent Revident LC/Q-TOF System User Guide...

  • Page 20: Side View

    Hardware Overview Side view Side view Inlet Source Heater Source HV Source gases Figure 3. Side view of Revident LC/Q-TOF Agilent Revident LC/Q-TOF System User Guide...

  • Page 21: Back View

    Hardware Overview Back view Back view Rough Pump Line Voltage RS485 Foreline Valve Remote Nitrogen Lifting Rough Vacuum Start Handles Figure 4. Back view of Revident LC/Q-TOF Agilent Revident LC/Q-TOF System User Guide...

  • Page 22: Instrument Status Indicator Lights

    Status LED = Off Power Switch LED = Blinking Yellow Instrument State = BACKGROUND SCAN Instrument State = Off Status LED = Green Status LED = Off Power Switch LED = Green Power Switch LED = Off Agilent Revident LC/Q-TOF System User Guide...

  • Page 23: Instrument Status Indicators In Acquisition

    Blue - Running, postrun: The instrument is currently collecting data. ⯀ Orange - Tuning: The instrument is in the process of adjusting MS parameters. ⯀ Red - Instrument Error: The instrument has an error and cannot process samples. Agilent Revident LC/Q-TOF System User Guide...

  • Page 24: Ion Sources

    (desorbed) into the gas phase. These ions are attracted to and pass through a capillary sampling orifice into the mass analyzer. Figure 5. ESI source with cover Agilent Revident LC/Q-TOF System User Guide...

  • Page 25: Agilent Jet Stream (Dual Ajs Esi) Source

    • Uses thermal gradient focusing technology, which is a process in which superheated nitrogen (N ) is used to improve ion generation and desolvation. • Improves sensitivity 5x or more for many small-molecule compounds that undergo electrospray ionization. Figure 6. Dual AJS ESI with cover Agilent Revident LC/Q-TOF System User Guide...

  • Page 26: Atmospheric Pressure Chemical Ionization (Apci) Source

    Because high temperatures are also involved, APCI is not appropriate for analysis of large biomolecules. APCI is used with normal-phase chromatography more often than electrospray is because the analytes are usually nonpolar. Figure 7. APCI source with cover Agilent Revident LC/Q-TOF System User Guide...

  • Page 27: Tuning

    Roles Configure Tune Calibration and Tuning Options Starting the Instrument Mass Calibration Checktune Autotune Generating a Detailed Tune Report Advanced Tune Diagnostic Tune Scheduling Tunes Scheduling a Mass calibration or Checktune Scheduling an Autotune Agilent Revident LC/Q-TOF System User Guide...

  • Page 28: About Tuning

    All tunes except for the Diagnostic tune can be scheduled to maximize instrument uptime. With MassHunter Acquisition12.0 for LC/Q-TOF software, the access to different tune functionalities is controlled via roles and privileges. Agilent Revident LC/Q-TOF System User Guide...

  • Page 29: Roles

    Lab Manager role has the privilege to configure tunes. Other roles do not have access to this configuration and are only able to perform tunes from a predefined list configured by the Lab Manager. See MassHunter Control Panel help for more information on authentication, roles, and privileges. Agilent Revident LC/Q-TOF System User Guide...

  • Page 30: Configure Tune

    Figure 8. Tune Status 5 When clicked, tune setpoints are applied. If an action is not selected within the timeout period of three minutes, the system will go back to the method setpoints. Agilent Revident LC/Q-TOF System User Guide...
  • Page 31 The Q-TOF system tune is always selected as this tune uses the default values as NOTE the starting point and has the all elements involved in mass resolution tuning included. 8 Select the System Settings drop-down list to view the changes. Agilent Revident LC/Q-TOF System User Guide...
  • Page 32 TOF tuning parameters. In addition to these predefined instrument modes/tunes, the lab manager role allows the creation of manual tunes that are made visible to operator and lab technician, if needed. Agilent Revident LC/Q-TOF System User Guide...

  • Page 33: Calibration And Tuning Options

    38. Table 2 to select the correct tuning mix for the source installed. Table 2 Tuning Mixes Source Tuning mix Part Number ESI/AJS ESI-X Tuning Mix 5191-6449 APCI APCI-L Low Concentration Tuning Mix G1969-85010 Agilent Revident LC/Q-TOF System User Guide...

  • Page 34: Starting The Instrument

    On the ribbon, click Instrument Status in the Windows section. 3 Hover over the Q-TOF device pane and click On. 4 Monitor baseline and adjust the plot if necessary. See “Monitoring baseline and adjusting plot” on page 55. Agilent Revident LC/Q-TOF System User Guide...

  • Page 35: Mass Calibration

    2 Click Tune > Mass Calibration in the left pane. 3 Click Tune control. 4 Select Mass Calibration. 5 Click Mass calibration/Checktune. When the tune completes, review the report. 6 Click Tune control to release control of the LC/Q-TOF instrument. Agilent Revident LC/Q-TOF System User Guide...

  • Page 36: Checktune

    Only the active instrument mode can be calibrated. NOTE 1 In the Method Editor window, click the MS Q-TOF tab. 2 Click Tune > Mass Calibration in the left pane. 3 Click Tune control. 4 Select Checktune. Agilent Revident LC/Q-TOF System User Guide...
  • Page 37 6 Click Mass calibration/Checktune. View the progress of the tune in the Real Time tune results. 7 When the tune completes, review the report. 8 Click Tune control to release control of the LC/Q-TOF instrument. Agilent Revident LC/Q-TOF System User Guide...

  • Page 38: Autotune

    5 (Optional) Activate Show realtime tune view to view the real time data acquisition. 6 Click Autotune. 7 When the tune completes, review the report. 8 Click Tune control to release control of the LC/Q-TOF instrument. Agilent Revident LC/Q-TOF System User Guide...

  • Page 39: Generating A Detailed Tune Report

    5 Use the toggles to select reports for viewing. 6 Click View tune reports generate PDFs for viewing in a PDF viewer. 7 Click Tune control in the toolbar to release control of the Q-TOF instrument. Agilent Revident LC/Q-TOF System User Guide...

  • Page 40: Advanced Tune

    1 In Acquisition, in the Method Editor window, click the MS Q-TOF tab. 2 Click Tune > Advanced Tune in the left pane. 3 Click Tune control. 4 Click Launch Advanced Tune. The Advanced Tune View opens. 5 Click the Tune tab. Agilent Revident LC/Q-TOF System User Guide...
  • Page 41 Tuning Advanced Tune 6 Click the Manual Tune tab. 7 Select a tune file and make changes. 8 Click to Save the tune file. Agilent Revident LC/Q-TOF System User Guide...

  • Page 42: Diagnostic Tune

    The Progress bar and Tune status window displays the progress and status of the tune. 5 In MassHunter Control Panel, navigate to the Control Panel > Administration > Diagnostics page. The log is named: YYY-MM-DD-h-m-sec-MSR.zip Agilent Revident LC/Q-TOF System User Guide...

  • Page 43: Scheduling Tunes

    The Schedule Tune dialog box opens. 5 Select Mass calibration/Checktune in the left pane. The right pane shows the information for scheduling a tune. 6 Click the Scheduling slider to switch on Scheduling. 7 Select either Weekly or Monthly. Agilent Revident LC/Q-TOF System User Guide...
  • Page 44 Tuning Scheduling a Mass calibration or Checktune 8 Select other options to indicate how often to schedule the mass calibration or checktune. 9 Click OK. Agilent Revident LC/Q-TOF System User Guide...

  • Page 45: Scheduling An Autotune

    The Schedule Tune dialog box opens. 5 Select Autotune in the left pane. The right pane shows the information for scheduling a tune. 6 Click the Scheduling slider to switch Scheduling on. 7 Select either Weekly or Monthly. Agilent Revident LC/Q-TOF System User Guide...
  • Page 46 Scheduling an Autotune 8 Select other options to indicate how often to schedule the Autotune or Transmission Tune. 9 Click OK. 10 Click Tune control in the toolbar to release control of the Q-TOF instrument. Agilent Revident LC/Q-TOF System User Guide...
  • Page 47 Viewing the System Activity Log for events and errors Setting Up an Acquisition Method Setting Up and Running a Single Sample Setting Up and Running a Worklist Reviewing Results with Qualitative Analysis Analyzing Data Agilent Revident LC/Q-TOF System User Guide...

  • Page 48: Basic Operation Overview

    LC/MS LC modules Edit DA Edit Q Set up and run a Edit the LC method interactive method parameters parameters parameters samples Set up and Review results with Review results Qualitative Quantitative Analysis Analysis Agilent Revident LC/Q-TOF System User Guide...

  • Page 49: Starting Up And Shutting Down The Instrument

    2 In Acquisition, in the Method Editor window, click the MS Q-TOF tab. 3 Click Tune > Advanced Tune in the left pane. 4 Click Tune control. 5 Click Launch Advanced Tune. Agilent Revident LC/Q-TOF System User Guide...
  • Page 50 8 Wait until the instrument completely vents (ensure that the Rough Vacuum reads approximately 760 Torr). 9 Manually close the foreline valve by twisting clockwise until hand tight. 10 Close the Acquisition program and shut down the computer. Agilent Revident LC/Q-TOF System User Guide...
  • Page 51 • In the wiring between the LC/MS instrument power cord and the AC power supply. • In the AC power supply • In the wiring from the AC power supply to the power switch. Agilent Revident LC/Q-TOF System User Guide...

  • Page 52: Starting The Masshunter Data Acquisition Software

    > Agilent Technologies > Control Panel. 2 In Control Panel, select an instrument and click Launch to open the project in Acquisition. For more information, refer to the MassHunter Control Panel help or the Introduction Workbook. Agilent Revident LC/Q-TOF System User Guide...

  • Page 53: Masshunter Data Acquisition Windows

    • To show or hide a window, click the window name in the Windows group of the ribbon. • Hide a window by clicking the X icon in the upper right corner of the window. Agilent Revident LC/Q-TOF System User Guide...
  • Page 54 “Instrument Status Indicators in Acquisition” on page 23. Actuals window This window displays the current value of selected instrument parameters. See “Set up to view real-time parameter values (actuals).” on page 60 for more information. Agilent Revident LC/Q-TOF System User Guide...
  • Page 55 Chromatogram Plot window. The signals and their display characteristics can be adjusted as described in this section. The customizations made to the plot remain, even when the Acquisition software is closed and reopened. Agilent Revident LC/Q-TOF System User Guide...
  • Page 56 Method Editor window. 3 Click OK to close the Edit Signal Plot dialog box. Spectrum window This window displays the spectral plot in real time. Additional tabs are displayed for other modules. Agilent Revident LC/Q-TOF System User Guide...
  • Page 57 Basic Operation MassHunter Data Acquisition Windows Method Editor window In this window, enter the acquisition parameters for the method. Tune values are displayed on the Autotune tab. Agilent Revident LC/Q-TOF System User Guide...
  • Page 58 Select whether to run Acquisition Only or Both Acquisition and DA for the Part of method to run option in the Worklist Run Parameters dialog box. Agilent Revident LC/Q-TOF System User Guide...

  • Page 59: Preparing The Lc Modules

    In the Method Editor window, click Apply in the toolbar to download the LC parameters to the LC. b Right-click an LC module in the Instrument Status window and select one of the commands to change any non-method control parameters, if needed. Agilent Revident LC/Q-TOF System User Guide...
  • Page 60 The Actuals window can be customized to display instrument conditions that you may want to view. a Click Actuals in the ribbon to display the Actuals window. b Right-click the Actuals window and select Setup. c Select parameters to appear in the Actuals window. Agilent Revident LC/Q-TOF System User Guide...
  • Page 61 Select any parameter in the Parameters to display list that you do not want included, and click Remove. f Customize the background color and the text color for any parameters in the Parameters to display list. Agilent Revident LC/Q-TOF System User Guide...
  • Page 62 Acquisition software and reopen it. 5 Set up real-time plot displays. As the column is conditioning, set up the displays to monitor the effluent. a In the Chromatogram Plots window, right-click the chromatogram plot, and select Change. Agilent Revident LC/Q-TOF System User Guide...
  • Page 63 Basic Operation Preparing the LC Modules b In the Edit Signal Plot dialog box, select the type of display signal, and click Agilent Revident LC/Q-TOF System User Guide...

  • Page 64: Preparing The Q-Tof Lc/Ms

    If you do monitor the LC baseline with a VWD or DAD, change back to the default instrument displays. 1 Right-click the chromatogram plot, and click Change. 2 Select the MS signal, and click OK. Agilent Revident LC/Q-TOF System User Guide...

  • Page 65: Select The Q-Tof/Tof Ion Source

    Ribbon. Or, click the Method layout in the Ribbon. 2 Select the MS Q-TOF tab. 3 Select the Method > Source section. 4 From the Ion Source drop-down list, select the ion source. 5 Click Apply Method in the Method Editor toolbar. Agilent Revident LC/Q-TOF System User Guide...

  • Page 66: Internal Reference Mass Correction

    Ribbon. Or, click the Method layout in the Ribbon. 2 Select the MS Q-TOF tab. 3 Select the Method > Source > Ref Mass section, and set the following parameters: • Select Enable Ref Mass correction. • Select Use Bottle A. Agilent Revident LC/Q-TOF System User Guide...
  • Page 67 Use default values or choose two masses spread across the application mass NOTE range. To add new values to the Reference Masses table, click Add row at the end and enter the exact mass values. 6 When finished, save the acquisition method. Agilent Revident LC/Q-TOF System User Guide...
  • Page 68 Internal Reference Mass Correction 7 Create a worklist of the samples to be analyzed and specify the acquisition method created in Steps 2 to 4. For a single sample, click Apply before starting the interactive run. Agilent Revident LC/Q-TOF System User Guide...

  • Page 69: Viewing The System Activity Log For Events And Errors

    Viewing the System Activity Log for events and errors The System Activity Log provides information regarding errors to assist with troubleshooting. To view the log, click System Activity Log in the ribbon of the Acquisition program, and view the logged events. Agilent Revident LC/Q-TOF System User Guide...

  • Page 70: Setting Up An Acquisition Method

    On the Acquisition tab, select the acquisition mode from the list in the System Settings table. The Acquisition Parameters table is changed accordingly when you change the acquisition mode. The parameters available on the right change depending on the acquisition mode. Agilent Revident LC/Q-TOF System User Guide...
  • Page 71 4 Set up to change instrument parameters with segments: a To add a segment, click Add Row or Insert Row. b Enter the parameters for each Scan segment. 5 Optional. Set up signals for the Chromatogram plot: a Click Chromatograms. Agilent Revident LC/Q-TOF System User Guide...
  • Page 72 Qual tab and the Quant Automation check box is marked on the Quant tab. a Select the DA tab. b Optional. For information on the Data Conversions tab, refer to the online Help. Agilent Revident LC/Q-TOF System User Guide...
  • Page 73 Method Path/Template File to use. If you want to print the report, mark the Print to default printer check box. Mark the Publish check box to create a CSV file, a TXT file, or a PDF file. Agilent Revident LC/Q-TOF System User Guide...
  • Page 74 Reason for creating a new version of this method. Refer to the MassHunter Control Panel online Help for more information. Click OK. c If necessary, name the method and click OK. Agilent Revident LC/Q-TOF System User Guide...

  • Page 75: Setting Up And Running A Single Sample

    DA or Acquisition Only for the Part of Method to run. If using Both Acquisition and DA Part of Method to run, the Override DA method is NOTE required. 4 To start the single sample run, click Run in the Sample Run window toolbar. Agilent Revident LC/Q-TOF System User Guide...

  • Page 76: Setting Up And Running A Worklist

    3 Enter all the information on the Sample Information tab. 4 On the Sample Position tab, specify the sample vial locations (make sure the specific sample tray type has been configured by right-clicking the autosampler device image). Agilent Revident LC/Q-TOF System User Guide...
  • Page 77 Setting Up and Running a Worklist 5 Specify the locations, and click OK. 6 To set up the worklist run, click Worklist Run Parameters 7 On the Worklist Run Parameters tab, type the paths for the method. Agilent Revident LC/Q-TOF System User Guide...
  • Page 78 The DA part of this method is used instead of the DA part of the current method. Or, select this method in the Add Multiple Samples dialog box. Agilent Revident LC/Q-TOF System User Guide...

  • Page 79: Reviewing Results With Qualitative Analysis

    • Select the most appropriate precursor and product ions for MS/MS analyses • Find compounds • Identify compounds • Molecular feature extraction Refer to the online Help for the Qualitative Analysis software to learn more. Agilent Revident LC/Q-TOF System User Guide...

  • Page 80: Analyzing Data

    • Identify and use outliers to change the method and requantitate the data using a better calibration curve fit or other more appropriate settings Refer to the online Help for the Quantitative Analysis program to learn how to do more operations to analyze your data.f Agilent Revident LC/Q-TOF System User Guide...

  • Page 81: Maintenance

    Replacing the corona needle Disinfection Source Maintenance Changing to a source that uses a different tuning mix Installing and removing sources Electrospray Ionization (ESI) source Agilent Jet Stream (AJS ESI) source Atmospheric Pressure Chemical Ionization (APCI) source Agilent Revident LC/Q-TOF System User Guide...

  • Page 82: Maintenance Overview

    This Maintenance chapter is broken down into maintenance procedures listed by routine, then periodic maintenance tasks with their recommended schedule of performance, with the EMF procedures cross referenced in the final section. Consult the online help or contact your Agilent consultant for further information. Agilent Revident LC/Q-TOF System User Guide...

  • Page 83: Routine Tasks

    5 Open the spray chamber. 6 Dampen a clean lint-free cloth with a mixture of LC/MS-grade isopropanol and LC/MS-grade water. 7 Wipe the spray shield and the area around the spray shield. 8 Close the spray chamber. Agilent Revident LC/Q-TOF System User Guide...

  • Page 84: Checking And Filling The Calibrant Bottle - Weekly

    4 Put the intake tube into the calibrant bottle as you lift the calibrant bottle into position. Do not touch the intake tube unless you are wearing clean gloves. C AU T I O N Agilent Revident LC/Q-TOF System User Guide...

  • Page 85: Cleaning The Spray Chamber - Weekly

    2 Disconnect the nebulizer sample line and sample nebulizer gas tubing from the nebulizer. 3 Disconnect all cables and tubings from the instrument. 4 Open the latch of spray chamber. 5 Open the spray chamber. 6 Put the source under a fume hood. Agilent Revident LC/Q-TOF System User Guide...
  • Page 86 C AU T I O N system performance. 24 Starting at the base of the needle, wipe the needle with a clean lint-free cloth. The cloth can be dry or dampened with LC/MS-grade isopropanol. Agilent Revident LC/Q-TOF System User Guide...
  • Page 87 Do not hit the tip of the corona needle. The tip can bend, which can decrease Clean and install the corona needle. C AU T I O N system performance. 25 Connect the nebulizer sample line and sample nebulizer gas tubing to the nebulizer. Agilent Revident LC/Q-TOF System User Guide...

  • Page 88: Flushing The Nebulizer - Weekly

    (beaker, bottle, etc.). 4 Control the LC flow through the acquisition software to pull from the solvent bottles. 5 Set the flow rate to not more than 1 mL per minute. Agilent Revident LC/Q-TOF System User Guide...
  • Page 89 Maintenance Flushing the nebulizer - weekly 6 Flush for 3 to 5 minutes. For heavy build up, additional flushing may be required. Agilent Revident LC/Q-TOF System User Guide...

  • Page 90: Periodic Tasks

    1 The oil level should be between the marks for Max and Min. 2 Check that the pump oil is clear and the color is lighter than amber. 3 If the pump oil is dark or full of suspended particles, replace it. Agilent Revident LC/Q-TOF System User Guide...

  • Page 91: Draining And Replacing The Ms-40+ Pump Oil And Replacing The Filter

    6 Place a tank for waste oil, for example, a small plastic tub under the oil drain plug. 7 Unscrew the oil fill cap, rotating it counterclockwise using a 10 mm hex key. Remove it from the instrument and place it in a secure location. Agilent Revident LC/Q-TOF System User Guide...
  • Page 92 9 Connect the exhaust tube. 10 Plug in the rough pump and all power cords from the LC/MS instrument. 11 Press the front power switch to begin pump down, returning the instrument to normal operation. Agilent Revident LC/Q-TOF System User Guide...

  • Page 93: Hepa Filter

    1 Release the clamps of the HEPA filter canister. 2 Open the lid. 3 Remove the filter. 4 Insert the new filter into the filter canister. 5 Close the lid. 6 Fasten the clamps. Agilent Revident LC/Q-TOF System User Guide...

  • Page 94: Replacing The Nitrogen Gas Filters

    Failure to connect the trap immediately may cause contamination of the C AU T I O N adsorbents inside. Reduced adsorption capacity will contribute to elevated levels of contaminants observed by the detector. This may result in the need for additional purge time. Agilent Revident LC/Q-TOF System User Guide...
  • Page 95 11 Secure the trap in a vertical orientation. The length of the trap should stand perpendicular to the floor. Wait for at least 3 minutes to purge out any air that may have entered the instrument tubing line. The trap is now ready for use. Agilent Revident LC/Q-TOF System User Guide...

  • Page 96: General Maintenance Tasks

    2 Determine your nebulizer type per the user guide or the document that comes with the kit. 3 Install the nebulizer in the Nebulizer Adjustment Fixture, turning clockwise to secure the nebulizer to the adjustment fixture. Agilent Revident LC/Q-TOF System User Guide...
  • Page 97 16 Hold the needle holder steady with a 1/4-inch x 5/16-inch open-end wrench, depending on your nebulizer holder style. 17 Tighten the union one-half to three-quarters of a turn, or until an audible squeak is heard. The ferrule is now compressed. Agilent Revident LC/Q-TOF System User Guide...

  • Page 98: Inspecting The Nebulizer

    6 Push the LED Flashlight into the Nebulizer Adjustment Fixture. No tool is needed. 7 Turn on the LED Flashlight. The on/off switch is at the end of the flashlight. Agilent Revident LC/Q-TOF System User Guide...
  • Page 99 For the APCI nebulizer, if needed, remove the pocket clip to focus properly. Table 1 Correct Needle Position Nebulizer Distance Microflow Nebulizer Assembly 0.003 inch ES Tested Nebulizer 0.000 (flush) Tested ESI Nebulizer Assembly (older style) 0.003 inch Tested APCI Nebulizer Assembly 0.000 (flush) Agilent Revident LC/Q-TOF System User Guide...

  • Page 100: Adjusting Or Replacing The Nebulizer Needle

    11 Carefully remove the new needle from the shipping tube and adhesive strip. Identify the sharp end of the needle. 12 Carefully slide the sharp end of the needle through the ferrule until 1 cm of the needle remains at the ferrule. Agilent Revident LC/Q-TOF System User Guide...
  • Page 101 18 Insert the needle into the nebulizer shaft. 19 Adjust the electrospray needle position before reinstalling the nebulizer in the spray chamber. 20 Tighten the fastener (T6 Torx screw) that secures the needle holder in place. Agilent Revident LC/Q-TOF System User Guide...

  • Page 102: Installing The Capillary

    7 Install the two T10 Torx screws to hold the end plate. 8 Lubricate the capillary tip with LC/MS-grade isopropanol. 9 Install the capillary cap over the outer end of the capillary. 10 Install the spray shield. Agilent Revident LC/Q-TOF System User Guide...
  • Page 103 Maintenance Installing the capillary 11 Close the spray chamber. Agilent Revident LC/Q-TOF System User Guide...

  • Page 104: Removing The Capillary

    10 Loosen the collet clamping knob slightly to loosen the capillary from the capillary puller tool. 11 Pull out the capillary from the collet. 12 Unscrew the tool from the shield mount. Agilent Revident LC/Q-TOF System User Guide...

  • Page 105: Cleaning The Capillary

    Sonication should be done under a fume hood. C AU T I O N 7 Rinse the capillary and graduated cylinder several times with de-ionized water. 8 Fill the graduated cylinder with de-ionized water and sonicate for 10 to 15 minutes. Agilent Revident LC/Q-TOF System User Guide...
  • Page 106 Cleaning the capillary 9 Remove the ion transport capillary from the graduated cylinder and remove the pipette tips (if any were used). 10 Blow out excess water from the ion transport capillary bore with nitrogen. Agilent Revident LC/Q-TOF System User Guide...

  • Page 107: Cleaning A Clogged Capillary

    2 Remove and replace the capillary with a new unit, or re-insert the capillary after cleaning and drying. 3 Monitor the rough vacuum level to ensure that it does not fall below 1.4 Torr. Agilent Revident LC/Q-TOF System User Guide...

  • Page 108: Flushing The Calibrant Delivery System (Cds)

    Fill the rinsed CDS bottle with approximately 20 to 30 mL of the acetonitrile/water mixture. b Install the bottle and turn on the calibrant flow. c Run the calibrant flush for at least 10 min. 6 Re-attach the sample tubing to the top of the nebulizer. Agilent Revident LC/Q-TOF System User Guide...
  • Page 109 8 Discard any remaining solvent and add fresh tune solution to the bottle. 9 Flush the fresh tune solution through the CDS for 5 min. Monitor tune ions to ensure that that response is adequate. Agilent Revident LC/Q-TOF System User Guide...

  • Page 110: Replacing The Ms Selection Valve Rotor Seal

    6 Use the 9/64-inch hex key to fix the three hex head screws from the stator face of the selection valve. 7 Plug in all the connections of the MS selection valve. 8 Close the CDS door. Agilent Revident LC/Q-TOF System User Guide...

  • Page 111: Cleaning The Corona Needle

    5 Starting at the base of the needle, wipe the needle with a clean lint-free cloth. The cloth can be dry or dampened with LC/MS-grade isopropanol. 6 Reinstall the corona needle assembly in the spray chamber. Agilent Revident LC/Q-TOF System User Guide...

  • Page 112: Replacing The Corona Needle

    Do not hit the tip of the corona needle. The tip can bend, which can decrease C AU T I O N the system. 7 Reinstall the corona needle assembly in the spray chamber. Agilent Revident LC/Q-TOF System User Guide...

  • Page 113: Disinfection

    8 Allow all surfaces to completely air-dry. No moisture should be present on the instrument surfaces. 9 Discard the gloves and other personal protective equipment or clean them in an approved process, then wash your hands. Agilent Revident LC/Q-TOF System User Guide...

  • Page 114: Source Maintenance

    (see “Mass Calibration” on page 35). • If the tune report does not show good results, tune the instrument. If needed, change the source to one that supports autotune. Agilent Revident LC/Q-TOF System User Guide...
  • Page 115 In the tune view or context, turn down the gas temperature as low as possible. WAR N IN G Leave the gas flow on for 30 minutes to cool the source. Once complete, proceed to the desired source removal and installation instructions. Agilent Revident LC/Q-TOF System User Guide...

  • Page 116: Electrospray Ionization (Esi) Source

    4 Install the source. 5 Close the source. 6 Connect the MultiMode cable delivering AJS nozzle voltage. 7 Connect the heater cable. 8 Connect the sample nebulizer gas tubing. 9 Connect the nebulizer sample line. Agilent Revident LC/Q-TOF System User Guide...
  • Page 117 4 If the checktune results are not acceptable, run autotune on a supported tuning source. 5 When the instrument is ready, load or create the method to use with the ion source. 6 Change the method settings for the new ion source. Agilent Revident LC/Q-TOF System User Guide...

  • Page 118: Agilent Jet Stream (Ajs Esi) Source

    3 Connect the MultiMode cable delivering AJS nozzle voltage. 4 Connect the heater cable. 5 Connect the sample nebulizer gas tubing. 6 Connect the nebulizer sample line. 7 Connect the reference nebulizer sample line and sample nebulizer gas tubing. Agilent Revident LC/Q-TOF System User Guide...
  • Page 119 3 Do a complete autotune. If an autotune is not available, do a checktune. If the tune report does not show good results, tune the instrument. If needed, change the source to one that supports autotune. Agilent Revident LC/Q-TOF System User Guide...

  • Page 120: Atmospheric Pressure Chemical Ionization (Apci) Source

    3 Rotate the ESI spray shield clockwise until the hole is in the correct position. 4 Gently tighten the Torx screws again. 5 Install the ion source, sliding it along the axis of its hinges. 6 Close the spray chamber. 7 Close the spray chamber latch. Agilent Revident LC/Q-TOF System User Guide...
  • Page 121 3 Do a complete autotune. If an autotune is not available, do a checktune. If the tune report does not show good results, tune the instrument. If needed, change the source to one that supports autotune. Agilent Revident LC/Q-TOF System User Guide...
  • Page 122 This page intentionally left blank. Agilent Revident LC/Q-TOF System User Guide...
  • Page 123 © Agilent Technologies, Inc. 2024 February 2024 D0110335 Revision A.00...

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