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3
Tuning
About Tuning
About Tuning
When the mass spectrometer (MS) is used as a detector for a liquid
chromatograph, a mass spectrum is associated with each data point in the
chromatogram. To obtain high quality, accurate mass spectra, the MS must be
optimized to:
Tuning is the process of adjusting MS parameters to achieve these goals.
Tuning is primarily concerned with finding the correct settings for the parameters
that control the transmission, filtering, and detection of ions. The Q-TOF/TOF
Tune section within MassHunter acquisition software allows the tuning and
mass calibration of the instrument. It includes the following general information
and settings, plus tabs for setting extra parameters and performing various
actions such as autotuning and manual tuning.
Tuning is accomplished by introducing a calibrant into the LC/MS and generating
ions. Using these ions, the tune parameters are then adjusted to achieve
sensitivity, resolution, and mass assignment goals. With a few exceptions, the
parameters that control ion formation are not adjusted. They are set to fixed
values known to be good for generating ions from the calibrant solution.
There are several different types of tunes (autotune, mass calibration/checktune,
advanced, and diagnostic) that can be performed on the instrument. All tunes
except for the Diagnostic tune can be scheduled to maximize instrument uptime.
With MassHunter Acquisition12.0 for LC/Q-TOF software, the access to different
tune functionalities is controlled via roles and privileges.
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•
Maximize sensitivity
•
Maintain an acceptable resolution
•
Ensure accurate mass assignments.
Agilent Revident LC/Q-TOF System User Guide
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