Thermo Scientific invitrogen E-Gel G8121ST User Manual page 34

NGS library size selection reference
Library Size Target
library
peak
Ion S5™ XL System
600-base-read 680 bp
Ion PGM™ System
400-base-read 480 bp
300-base-read 390 bp
200-base-read 330 bp
100-base-read 200 bp
Ion Proton™ System
200-base-read 270 bp
150-base-read 220 bp
34
Run time to Input
sample
reference
amount
line
500 ng
–
17.5 19 min
50 100 ng
–
500 ng
14–20 min
50–100 ng
500 ng
13–16 min
50–100 ng
500 ng
12–14 min
50–100 ng
500 ng
11–12.5 min
50–100 ng
500 ng
12–14 min
50–100 ng
500 ng
11–14.5 min
50–100 ng
Stop the run and collect your
sample when...
600 bp band has just completely
entered the top edge of the
collection well
700 bp band has just completely
entered the top edge of the
collection well
500 bp band is at the top of the
exposed agarose area
500 bp band has just entered the
top edge of the collection well
400 bp band is at the middle of
the exposed agarose area
500 bp band is at the top of the
exposed agarose area
350 bp band is at the top of the
exposed agarose area
350 bp band has just completely
entered the top edge of the
collection well
200 bp band is in the middle of
the collection well
200 bp band is in the middle of
the collection well
300 bp band is at the top of the
exposed agarose area
300 bp band is at the middle of
the exposed agarose area
250 bp band is at the middle of
the exposed agarose area
250 bp band is at the middle of
the exposed agarose area
E-Gel Power Snap Electrophoresis System User Guide
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