Thermo Scientific invitrogen E-Gel G8121ST User Manual page 5

Upgrade system firmware ............................................................................................................................ 39
Instrument Specifications ............................................................................................................................ 40
Instrument dimensions and specifications .............................................................................................. 40
Electrical requirements ............................................................................................................................ 41
Environmental requirements .................................................................................................................... 41
Appendix C ................................................................................................................... 42
E-Gel agarose gels ...................................................................................................................................... 42
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Choosing the right gel .................................................................................................................................. 43
Analytical gels ............................................................................................................................................ 43
Gels for preparative gel electrophoresis in Cloning and NGS applications ........................................... 43
Other available gel types for routine electrophoresis ............................................................................. 44
Opening E-Gel cassettes ............................................................................................................................ 44
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Gel Knife ..................................................................................................................................................... 44
Open an E-Gel cassette with a Gel Knife ................................................................................................ 45
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Cleaning and storage ................................................................................................................................ 45
E-Gel agarose gel disposal guidelines ................................................................................................... 45
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Appendix D ................................................................................................................... 46
Choosing the right DNA ladder .................................................................................................................... 46
Appendix E ................................................................................................................... 47
Running RNA Samples on E-Gel
Denaturing agents ..................................................................................................................................... 47
Prepare ladder ........................................................................................................................................... 47
Denature sample ....................................................................................................................................... 47
Typical result of RNA separation on E-Gel
Appendix F ................................................................................................................... 48
E-Gel Power Snap Blue-Light Transilluminator ....................................................................................... 48
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Imaging E-Gels on Third Party Gel Imagers ............................................................................................ 48
Nucleic acid stain use in E-Gel
SYBR Safe DNA Gel Stain ........................................................................................................................ 49
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Safety features ........................................................................................................................................... 49
Cloning benefits ......................................................................................................................................... 49
Disposal ...................................................................................................................................................... 49
Spectrum .................................................................................................................................................... 49
Visualization ............................................................................................................................................... 50
SYBR Gold II Gel Stain ............................................................................................................................. 50
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Disposal ...................................................................................................................................................... 50
Spectrum .................................................................................................................................................... 50
Visualization ............................................................................................................................................... 50
Appendix G ................................................................................................................... 51
Instrument starter kits ................................................................................................................................. 51
E-Gel agarose gels ...................................................................................................................................... 53
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Accessory products ....................................................................................................................................... 55
E-Gel Power Snap Electrophoresis System User Guide
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EX Agarose Gels ................................................................................... 47
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EX agarose gels ................................................................. 47
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agarose gels ............................................................................................ 49
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