Thermo Scientific invitrogen E-Gel G8121ST User Manual page 37

Observation
Sample leaking from the
wells
DNA sample cannot be
seen
RNA sample cannot be
seen
Speckles visible
High background,
suboptimal, or no image
(when used with E-Gel
Power Snap Camera)
High background,
suboptimal, or no image
(when used with E-Gel
™
Imager)
Low cloning efficiency
Bubbles appear in gel
cassette
E-Gel Power Snap Electrophoresis System User Guide
™
Cause
Sample is overloaded
Wells damaged during comb
removal
Inhibition of visualization by heat
Inhibition of visualization by heat
and denaturing agent
Dust fluorescing in same
wavelength as SYBR Safe /
™
SYBR Gold II
™
Incorrect camera adjustments
No filters or wrong filter set
Photographic settings not
optimal
E-Gel agarose gels with
™
ethidium bromide are not
compatible for visualization on a
blue light transilluminator
Used a UV light source to
visualize DNA
Extended run time causes
excessive temperature in gel
matrix
Recommended action
Load the recommended sample volume per well.
Remove the gel comb gently without damaging the
wells.
Wait 10–15 minutes for gel to cool before
visualization.
Wait 10–15 minutes for gel to cool before
visualization.
Make sure gel is clean before imaging.
Refer to the E-Gel Power Snap Camera use guide.
™
Refer to E-Gel Imager Technical Guide or
™
instrument manufacturer for optimal filter set.
Determine optimal settings empirically by
adjusting exposure time, gain, etc.
Use an E-Gel Imager with UV base or a 3
™
UV transilluminator.
For cloning applications, use E-Gel
Agarose Gels with SYBR Safe; or for gel excision
use a blue light transilluminator, such as the Safe
Imager 2.0 Blue-Light Transilluminator (Cat. No.
™
G6600).
Allow the gel cool down, this does not affect the
final result.
-party
rd
CloneWell II
™ ™
37