Thermo Scientific Surveyor UV Plus Hardware Manual page 62

4
Troubleshooting
Detector-Related Problems
Table 7. Detector-related problems (Sheet 2 of 3)
Symptom Cause
Random noisy Contaminated flow cell
baseline
Leak in sample inlet line
Bubble trapped in flow cell
Leaking flow cell
Insufficient lamp warm-up
Aging or defective lamp
Ground loop problem between
integrator and detector
Dirty flow cell or lamps
Integrator input and output
voltage not matching
Excessive baseline Contaminated flow cell
drift
Mobile phase contamination
Material bleeding from column Clean or replace the column.
Leaks in system or flow cell
Tiny bubble trapped in the
flow cell
Excessive temperature
fluctuations
46 Surveyor UV/Vis Plus Detector Hardware Manual
Remedy
Flush the flow cell with cleaning solvents. See
Flow Cells" on page 35. Check for leaks.
Check all of the fittings for leaks, from the column outlet to
the flow cell inlet.
Increase the flow rate until the bubble is removed. Connect a
backpressure device to the flow cell (check pressure rating to
avoid rupturing the flow cell).
Replace the flow cell.
Allow a 30 minute warm-up for normal operation and a
1.5 hour warm-up for maximum sensitivity.
Replace the lamp.
Check the cable connections to the detector output; do not
ground at both ends of the cable.
Clean the dirty component. See
page 35.
Verify that the integrator is connected to the appropriate
analog output connections on the back panel of the detector.
See "Connecting the Analog Outputs"
Check the attenuation setting on the integrator.
Flush the flow cell with cleaning solvents. See
Flow Cells" on page 35. Check for leaks.
Replace the mobile phase with fresh mobile phase that is
made with high-purity solvents.
Check all of the fittings for leaks. Replace the flow cell.
Increase the flow rate until the bubble is removed. Connect a
backpressure device to the flow cell outlet (check backpressure
rating to avoid rupturing flow cell).
Remove the system from drafts. Thermostatically control the
column temperature.
"Cleaning the
"Cleaning the Flow Cells"
on page 20.
"Cleaning the
Thermo Scientific
on