Thermo Scientific Heracell Vios 160i CR Operating Instructions Manual
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Thermo Scientific Heracell Vios 160i CR Operating Instructions Manual

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Thermo Scientific
Heracell Vios 160i CR/
Heracell Vios 250i CR
CO
Incubator
2
Operating Instructions
50163129
Rev. B
June 2021

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Summary of Contents for Thermo Scientific Heracell Vios 160i CR

  • Page 1 Thermo Scientific Heracell Vios 160i CR/ Heracell Vios 250i CR Incubator  Operating Instructions 50163129 Rev. B June 2021...
  • Page 2 Heracell Vios CR™, Steri-Run™, Forma™, Steri-Cycle CR™, iCan™, THRIVE™ and Cell Locker™ are registered trade- marks of Thermo Scientific. Thermo Scientific is a brand of Thermo Fisher Scientific Inc. All other trademarks mentioned in the operating instructions are the exclusive property of the respective manufacturers....

  • Page 3: Table Of Contents

    Optional Equipment Components Heracell Vios 160i / 250i CR ............. 1-3 Chapter 2 Installation of the Device....................2-1 Environmental Conditions ......................... 2-1 Requirements ..........................2-1 Site Ventilation........................... 2-2 Space Requirements..........................2-2 Transport ............................2-3 Stacking.............................. 2-4 Stacking Variants ........................... 2-7 Heracell Vios 160i / 250i CR Thermo Scientific...
  • Page 4 Installing the HEPA Filter in the Rear Panel ..................4-9 Installing the Shelf System........................4-9 Installation/Removal of the Support Rail ..................4-9 Shelf Support Installation ......................4-10 Levelling the Device ........................4-11 Heracell Vios 160i / 250i CR Thermo Scientific...
  • Page 5 Software Versions ........................6-41 Scaling the Trend Display......................... 6-41 Error Messages..........................6-43 Response to an Error Message Event .................... 6-43 Resetting Overtemperature Protection ..................6-44 Measures After Power Outage ...................... 6-45 Troubleshooting .......................... 6-46 Heracell Vios 160i / 250i CR Thermo Scientific...
  • Page 6 Chapter 10 Waste Disposal....................... 10-1 Overview of the Materials Used ......................10-1 Chapter 11 Specifications......................... 11-1 Heracell Vios 160i CR ........................11-2 Heracell Vios 160i CR ........................11-4 Heracell Vios 250i CR ........................11-6 Heracell Vios 250i CR ........................11-8 Chapter 12 Data Communication .....................
  • Page 7 Example of a Code for a a Data Logger Query ................12-16 Program Heracell Vios 160i / 250i CR ..................12-21 Installing the Heracell Vios 160i CR & Heracell Vios 250i CR ..........12-22 Operating the Heracell Vios 160i CR & Heracell Vios 250i CR ..........12-23 Chapter 13 Device Log ........................
  • Page 8 Table of Contents Heracell Vios 160i / 250i CR Thermo Scientific...
  • Page 9 Figure 3-8. Interfaces (Right Side of Control Box) ................3-13 Figure 3-9. Heracell Vios 160i / 250i CR with Glass Door..............3-17 Figure 3-10. Heracell Vios 160i CR with Threefold Gas-Tight Screen ..........3-18 Figure 3-11. Water Reservoir ........................3-19 Figure 3-12. Fill and Drain Valve of Water Reservoir................3-19 Figure 3-13.
  • Page 10 Figure 6-26. Interface USB Menu ......................6-24 Figure 6-27. USB Interface Baud Rate Setting ..................6-24 Figure 6-28. Language Setting.......................6-25 Figure 6-29. Choose the Desired Reminder Interval Function..............6-26 Figure 6-30. Setting the Reminder Interval for Steri-Run..............6-27 Heracell Vios 160i / 250i CR Thermo Scientific...
  • Page 11 Figure 9-4. Gas-Tight Screen Measurement Port..................9-5 Figure 9-5. CO2 Calibration Procedure ....................9-7 Figure 9-6. Removing the Airbox ......................9-8 Figure 9-7. Installing the HEPA Filter....................9-9 Figure 9-8. Gas Inlet Filter Installation....................9-11 Heracell Vios 160i / 250i CR Thermo Scientific...
  • Page 12 Figures Heracell Vios 160i / 250i CR Thermo Scientific...

  • Page 13: Preface

    General Notes Identification of the Device and of the Documentation Identification of the Device Identification data incubator Device name:  Heracell Vios 160i CR Unit Name: Heracell Vios 250i CR Allocation of product documentation Documents CD-ROM 50163127  Certifications and quality audit:...

  • Page 14: Applicability Of The Instructions

    • Keep these operating instructions close to the device so that safety instructions and important information are always accessible. Should you encounter problems that are not detailed adequately in these operating instructions, please contact Thermo Scientific immediately for your own safety. Warranty Requirements for Warranty...

  • Page 15: Explanation Of Safety Information And Symbols

    CAUTION Indicates a hazardous situation which, if not avoided, could result in minor or moderate injuries. CAUTION Indicates a situation which, if not avoided, could result in property damage. Note Is used for applicational hints and useful information. Heracell Vios 160i / 250i CR Thermo Scientific...

  • Page 16: Additional Symbols For Safety Information

    Preface Explanation of Safety Information and Symbols Additional Symbols for Safety Information: Wear safety gloves! Wear safety goggles! Harmful liquids! Electric shock! Hot surfaces! Fire hazard! Explosion hazard! Suffocation hazard! Tilting hazard! Heracell Vios 160i / 250i CR Thermo Scientific...

  • Page 17: Symbols On The Device

    To that end, a controlled set of physiological ambient conditions is produced in the workspace of the device by exerting precise control of the following parameters: • Temperature • CO content • O content • Relative humidity Heracell Vios 160i / 250i CR Thermo Scientific...

  • Page 18: Incorrect Use

    • IEC 60529:2.1:COR 2018 (IP54) • FCC 47, Part 15, § 15.107, EMC regulation FCC • FCC 47. Part 15, § 15.109, EMC regulation FCC For other countries, the applicable national regulations are binding. Heracell Vios 160i / 250i CR Thermo Scientific...

  • Page 19: Safety Notes On Gases

    ) released into the room atmosphere may cause suffocation. If CO is released, initiate safety measures immediately! • Leave the room immediately and do not allow others to enter the room! • Inform security service or fire department! Heracell Vios 160i / 250i CR Thermo Scientific...

  • Page 20: Safety Notes On Oxygen (O2)

    ) released into the room atmosphere may cause suffocation. If N released, initiate safety measures immediately! • Leave the room immediately and do not allow others to enter the room! • Inform security service or fire department! Heracell Vios 160i / 250i CR Thermo Scientific...

  • Page 21: Chapter 1 Delivery Of The Device

    If components are missing or damage is found on the device or the packaging, esp. damages caused by humidity and water, please contact the carrier as well as the customer service immediately. Heracell Vios 160i / 250i CR Thermo Scientific...

  • Page 22: Standard Equipment Components Heracell Vios 160I / 250I Cr

    Operating instructions Quick-release connector with hose for water draining Optional Equipment Components Heracell Vios 160i / 250i CR Components delivered with oxygen supply control option Pieces sensor head connecting hose set Heracell Vios 160i / 250i CR Thermo Scientific...

  • Page 23: Optional Equipment Components Heracell Vios 160I / 250I Cr

    Sixfold gas-tight screen Cell Locker Refill Funnel Optional Equipment Components Heracell Vios 160i / 250i CR Components delivered with 250 liter units with split inserts Pieces Support brackets Split inserts Sixfold gas-tight screen Heracell Vios 160i / 250i CR Thermo Scientific...
  • Page 24 Delivery of the Device Optional Equipment Components Heracell Vios 160i / 250i CR Heracell Vios 160i / 250i CR Thermo Scientific...

  • Page 25: Chapter 2 Installation Of The Device

    • Relative humidity up to 80% (max.). • No direct exposure to sunlight. • Devices that produce excessive amounts of heat must not be placed near the Heracell Vios 160i / 250i CR Heracell Vios 160i / 250i CR Thermo Scientific...

  • Page 26: Site Ventilation

    To ensure the accessibility for care and maintenance works, keep larger side and rear distances. The swivel-out for units with sixfold gas tight screen with Cell Locker must have a clearance to the wall directly at the hinge side of >= 31 cm / 12.2”. Heracell Vios 160i / 250i CR Thermo Scientific...

  • Page 27: Transport

    Lift the device only using the lift points. CAUTION Heavy loads! Lift with care! The weights without accessories are for Heracell Vios 160i CR - 95 kg and for Heracell Vios 250i CR - 119 kg. To avoid injury through physical strain, such as strain trauma and slipped discs, do not attempt to lift the incubator alone! Use the required number of persons or lifting aids according to the weight....

  • Page 28: Stacking

    Before moving stacked devices, be sure to remove the support brackets! Stacked devices provided with a mobile base rack must only be moved inside rooms on even floors without any slopes. Figure 2-3. Stacking Heracell Vios 160i / 250i CR Thermo Scientific...

  • Page 29: Figure 2-4. Stacking Adapter And Base Rack With Stacking Elements

    2. Place the lower device on the mobile base rack on the attachment points so that the rear side rests against the connecting straps. 3. Using the outer rear panel screws on the left and right side, attach the device to the connecting straps, only tightening the screws to hand-tight. Heracell Vios 160i / 250i CR Thermo Scientific...
  • Page 30 Condensate formation during the operation of stacked devices:  As a rule when operating stacked devices of type Heracell Vios 160i CR and Heracell Vios 250i CR, an adapter plate should be placed between the two devices for thermal separation....

  • Page 31: Stacking Variants

    Installation of the Device Stacking Stacking Variants Possible stacking combinations Bottom stacking adapter Heracell Vios 160i CR Heracell Vios 250i CR Steri-Cycle i160 CR Steri-Cycle i250 CR Heracell Vios 160i CR 50148171 Steri-Cycle i160 CR Heracell Vios 250i CR 50154522 Steri-Cycle i250 CR For details see assembly instruction of stacking adapter kit.

  • Page 32: Support Stand With Castors And Supporting Feet (160I And 250I Unit)

    Please screw in the supporting feet with a pipe wrench before using the support stand to move the unit. • Rotate out until the load is supported by the supporting feet and not by the castors. Heracell Vios 160i / 250i CR Thermo Scientific...
  • Page 33 Material Number Description 50161859 Support stand H = 76 mm with castors for Heracell Vios 160i CR and Steri-Cycle i160 CR 50162731 Support stand H = 76 mm with castors for Heracell Vios 250i CR and...

  • Page 34: Installing The Floor Stand, 780 Mm

    • Place the equipment with door handle side (blue line) upon the counter weight (50), the floor stand for 250L equipment has no counter weight. • Level the sub-frame with an open-end wrench (60): Heracell Vios 160i / 250i CR 2-10 Thermo Scientific...

  • Page 35: Retrofitting/Modifications

    Retrofitting/Modifications The following components can be retrofitted to the standard version: Heracell Vios 160i CR • threefold gas-tight screen (the gas-tight screen is installed in place of the glass door), • sixfold gas tight screen with or without Cell Locker, •...
  • Page 36 Installation of the Device Retrofitting/Modifications Heracell Vios 160i / 250i CR 2-12 Thermo Scientific...

  • Page 37: Chapter 3 Description Of The Device

    “Safety Devices Heracell Vios 160i / 250i CR” page 3-4 • “Workspace Atmosphere” page 3-5 • “Door Switch” page 3-10 • “Sensor System” page 3-10 • “Supply Interface” page 3-13 • “Workspace Components” page 3-16 Heracell Vios 160i / 250i CR Thermo Scientific...

  • Page 38: Heracell Vios 160I / 250I Cr Front View

    4. O sensor (optional) 5. Door handle for the glass door or the gas-tight screen 6. Glass door or gas-tight screen (optional) 7. Device door 8. Magnetic door seal, replaceable 9. Prefilter Heracell Vios 160i / 250i CR Thermo Scientific...
  • Page 39 20. iCan™ Touchscreen (Control panel) 21. Power switch 22. a = Electromechanical door lock / b - Closing hook on outer door 23. Alarm indicator in the rear wall filter enclosure for the HEPA filter Heracell Vios 160i / 250i CR Thermo Scientific...

  • Page 40: Heracell Vios 160I / 250I Cr Back View

    • The alarm relay circuit uses audible and visual alarms to indicate errors during operation. • An externally mounted light provides a visual warning of any malfunction of the internal particle extraction system. Heracell Vios 160i / 250i CR Thermo Scientific...

  • Page 41: Workspace Atmosphere

    For continuous operation, a sufficient quantity of processed water with the recommended water quality must be provided: • Max. fill volume for Heracell Vios 160i CR and Heracell Vios 250i CR: 3 l. Water Quality Recommendations For trouble-free operation, fill the water reservoir with sterilized distilled water or water treated to an equivalent quality level.

  • Page 42: Prefilter

    Heracell Vios 160i / 250i CR the rear wall filter enclosure which must be installed by the customer before starting up the device. Heracell Vios 160i / 250i CR Thermo Scientific...

  • Page 43: Figure 3-3. Hepa Filter And Airbox

    3-4) to the workspace ceiling, thus ensuring an optimum temperature distribution. At the same time, it directs the incoming stream of process gases into the workspace and ensures that the gases are optimally mixed and distributed uniformly. Heracell Vios 160i / 250i CR Thermo Scientific...

  • Page 44: Co2 Supply

    21 % to 90 %. Operation with higher oxygen concentrations requires that the fire protection caution notes in Preface in the section Safety Notes on Gases on page be heeded. Heracell Vios 160i / 250i CR Thermo Scientific...

  • Page 45: N2 Supply

    A HEPA filter with 12 hexagonal bolts must be installed in the rear wall filter enclosure before the device is commissioned. Note  Do not touch the surface of the HEPA filter. Back View Front View HEPA filter holder Exhaust filter Heracell Vios 160i / 250i CR Thermo Scientific...

  • Page 46: Door Switch

    Mounted in the rear wall of the workspace are the fan wheel and the sensor modules: • Sensor for the acquisition of the workspace temperature and the overtemperature protection (1/Figure 3-6). Heracell Vios 160i / 250i CR 3-10 Thermo Scientific...

  • Page 47: Figure 3-7. Sensors For Water Level

    6-43). Figure 3-7. Sensors for Water Level • Additionally, a mechanical/visual indicator for the maximum water level is available to assist the user in filling the water reservoir (see/Figure 3-7). Heracell Vios 160i / 250i CR Thermo Scientific 3-11...
  • Page 48 • the alarm relay responds. If the error message is accepted, the display shows the Overtemperature icon to indicate the response of the thermal protection, and the temperature display is highlighted in red. Heracell Vios 160i / 250i CR 3-12 Thermo Scientific...

  • Page 49: Supply Interface

    Description of the Device Supply Interface Supply Interface Standard Interfaces Figure 3-8. Interfaces (Right Side of Control Box) Heracell Vios 160i / 250i CR Thermo Scientific 3-13...

  • Page 50: Gas Connection

    16 bit per channel, equivalent to 65536 incremental values. The interface port accepts various customer-supplied external measurement devices with 4-20 mA inputs - for example, the Thermo Scientific Smart-Vue Wireless Monitoring System (not supplied). Heracell Vios 160i / 250i CR...
  • Page 51 Pin No. Channel Description Channel 1: Temperature Channel 1 Ground Channel 2: CO Channel 2 Ground Channel 3: O Channel 3 Ground Channel 4: Not used Channel 4 Ground Heracell Vios 160i / 250i CR Thermo Scientific 3-15...

  • Page 52: Alarm Contact

    Steri-Run sterilization routine is running. WARNING The HEPA filter cartridge is only resistant to temperatures up to 60°C, not autoclavable and must be removed prior to starting Steri-Run sterilization. Heracell Vios 160i / 250i CR 3-16 Thermo Scientific...

  • Page 53: Glass Door And Optional Gas-Tight Screen

    Glass Door and Optional Gas-Tight Screen The standard version of the Heracell Vios 160i CR and Heracell Vios 250i CR units is equipped with a single-piece door made of tempered safety glass.

  • Page 54: Water Reservoir

    Description of the Device Workspace Components Figure 3-10. Heracell Vios 160i CR with Threefold Gas-Tight Screen Each door of the gas-tight screen (2/Figure 3-10) is separately closed (1/Figure 3-10). Water Reservoir The water reservoir is recessed into the interior floor and separated from the workspace by means of a cover panel.

  • Page 55: Figure 3-11. Water Reservoir

    Plugging the drain tube supplied into the front-side quick-drain valve starts draining immediately. Figure 3-12. Fill and Drain Valve of Water Reservoir Heracell Vios 160i / 250i CR Thermo Scientific 3-19...

  • Page 56: Water Filling

    The outer door of the unit and the perimeter of the door opening are heated as well. The heat radiated onto the interior glass door/gas-tight screen prevents condensate formation. The workspace of the device always remains visible, despite high humidity. Heracell Vios 160i / 250i CR 3-20 Thermo Scientific...

  • Page 57: Rear Panel Openings

    Before starting the sterilization run, the two silicon plugs inside and outside the access port must be fitted on the access port. The silicon plug on the outside should cover the free space inside the access port. Heracell Vios 160i / 250i CR Thermo Scientific 3-21...

  • Page 58: Shelf System

    3-16) have an integrated tilt protection and withdrawal stop. Heracell Vios 250i CR models come equipped with a single-piece bottom insert, as shown on the left side in Figure 3-16, Heracell Vios 160i CR with a cutout for refilling (4/Figure 3-16). For details on using the shelf system, “Start-up”...

  • Page 59: Electromechanical Door Lock

    To reach it, remove the round cover inserted in the floor and replace it after unlocking. Detail C of Figure 3-17 presents a breakout view through the side panel to illustrate the lever location. Heracell Vios 160i / 250i CR Thermo Scientific 3-23...
  • Page 60 Description of the Device Electromechanical Door Lock Heracell Vios 160i / 250i CR 3-24 Thermo Scientific...

  • Page 61: Chapter 4 Start-Up

    Before the initial start-up, the device must be cleaned and disinfected.  Before the disinfection is performed, the following workspace components must be cleaned: • Support rail Heracell Vios 160i / 250i CR Thermo Scientific...

  • Page 62: Installing The "Max" Fill Level Indicator And The Prefilter

    (3/Figure 4-2) on the front side of the unit. 3. Suspend the “MAX” fill level indicator (1/Figure 4-1) in the slot provided in the water reservoir cover panel (5/Figure 4-1). Heracell Vios 160i / 250i CR Thermo Scientific...

  • Page 63: Figure 4-1. "Max" Fill Level Indicator

    Figure 4-1. “MAX” Fill Level Indicator 4. Check prefilter for damage and replace if necessary 5. Insert the prefilter (4/Figure 4-2) into the water reservoir cover panel. Figure 4-2. “MAX” Fill Level Indicator and Prefilter Heracell Vios 160i / 250i CR Thermo Scientific...

  • Page 64: Installing The Hepa Filter In The Rear Wall Filter Enclosure

    4-3) on the two rear wall studs and tilt the air duct backwards. 3. Lock the lateral keyholes of the top part (step G/Figure 4-3) into the retaining screws in the workspace ceiling. Heracell Vios 160i / 250i CR Thermo Scientific...

  • Page 65: Figure 4-3. Assembling The Air Duct

    Start-up Install the Air Duct Figure 4-3. Assembling the Air Duct Heracell Vios 160i / 250i CR Thermo Scientific...

  • Page 66: Installing The Hepa Filter And The Water Reservoir Cover Panel

    Figure 4-4. Air Duct Parts Heracell Vios 160i CR Note Do not remove the air duct seals on the Heracell Vios 160i CR versions. The rear wall air ducts are important for proper air flow on VIOS160 models with a stainless steel inner chamber.

  • Page 67: Figure 4-5. Assembling The Hepa Filter And The Airbox

    4-5) on the right side of the airbox into the HEPA filter catches (6/Figure 4-5). 4. Place the seal (8/Figure 4-5) into the groove (7/Figure 4-5) provided at the airbox tubing and press it down evenly. Heracell Vios 160i / 250i CR Thermo Scientific...

  • Page 68: Figure 4-6. Place The Airbox Onto The Water Reservoir Cover Panel

    9. Let the front edge of the water reservoir cover slide into the water reservoir (2/Figure 4-7). The airbox tubing slides into the fan outlet. Heracell Vios 160i / 250i CR Thermo Scientific...

  • Page 69: Installing The Hepa Filter In The Rear Panel

    The locksprings of the support rails must point upwards (see Figure 4-8 page 4-9). 1. Position the support rail onto the lower embossing and tilt it toward the workspace side wall so that the rail is positioned over the two embossings. Heracell Vios 160i / 250i CR Thermo Scientific...

  • Page 70: Shelf Support Installation

    4-9) ahead onto the shelf support. The tilt protection is also used as a guide for the shelf. 2. Slightly raise the shelf so that the withdrawal stop (6/Figure 4-9) can be routed over the shelf supports. Heracell Vios 160i / 250i CR 4-10 Thermo Scientific...

  • Page 71: Levelling The Device

    Note With the Heracell Vios 160i CR incubator be sure to install the shelf that has the cut-out for water refilling at the bottom position only. For the sixfold gas tight screen, the shelf supports are installed in the rectangular holes only.

  • Page 72: Reinforced Shelves

    With heavy loads applied (e.g. cell factories) the shelf may slightly deflect, but lesser than a standard shelf. Heracell Vios 160i / 250i CR 4-12 Thermo Scientific...

  • Page 73: Installing The Shelf Supports

    • Slightly bend the mounting outwards to enable the bar to snap with its tongues into the corresponding mounting slots. • Repeat this also for 2 or 3 other reinforcement bars Heracell Vios 160i / 250i CR Thermo Scientific 4-13...

  • Page 74: Installing The Reinforced Shelf

    Set the gas supply to a range between 0.8 bar (11.6 psi) min. and 1.0 bar (14.5 psi) max. and make sure that this pressure setting cannot be changed! Heracell Vios 160i / 250i CR 4-14 Thermo Scientific...

  • Page 75: Installing Gas Pressure Hoses

    To ensure permanent pressure compensation, the pressure compensation opening must not be connected to an exhaust air system. The pipe of the pressure compensation opening must not be extended or redirected. Heracell Vios 160i / 250i CR Thermo Scientific 4-15...

  • Page 76: Gas Connection

    Start-up Gas Connection Gas Connection Figure 4-12. Gas Connection The gas supply from the gas supply system to the device is established using the supplied flexible gas pressure hoses: Heracell Vios 160i / 250i CR 4-16 Thermo Scientific...

  • Page 77: Mains Connection

    5. Mains connection: The mains socket must be easily identifiable by the user and freely accessible at all times. The power cord plug is the all-pole disconnecting device from the mains supply. Heracell Vios 160i / 250i CR Thermo Scientific 4-17...

  • Page 78: Connecting The Usb Interface

    Connecting the Alarm Contact Note Skilled work: Thermo Scientific warrants the operational safety and the proper performance of the device only if installation and repairs are performed properly. The connection of the device to an external alarm system must only be carried out by adequately trained and authorized expert electrical/telecommunication personnel! Function:...

  • Page 79: Figure 4-14. Connection Example Alarm Contact

    Specifications for the operating voltage and the fusing of external alarm circuitry are given in the table above. 1. Wire the individual conductors as shown in the wiring diagram. Heracell Vios 160i / 250i CR Thermo Scientific 4-19...

  • Page 80: Connecting The 4-20 Ma Interface

    Figure 4-15. Pin-out of the 4-20 mA Interface Note Skilled work: Thermo Scientific warrants the operational safety and the proper performance of the device only if installation and repairs are performed properly. The connection of the device to an external alarm system must only be carried out by adequately...

  • Page 81: Chapter 5 Operation

    Steri-Run sterilization cycle. • Run the Steri-Run sterilization routine (see “Steri-Run Sterilization Routine” page 8-7) or disinfect the workspace according to the hygiene regulations set forth by the operator. Heracell Vios 160i / 250i CR Thermo Scientific...

  • Page 82: Starting Operation

    3-5. When the water fill level drops below the minimum value, the water must be refilled. Filling max. for the Heracell Vios 160i CR and Heracell Vios 250i CR: 3 l Starting Operation 1. Open glass door or gas-tight screen.

  • Page 83: Water Filling

    • Fill the funnel to the top to facilitate gravity to fill with sterile distilled water. This may take up to several times. • Keep adding until the maximum level indicator is reached. Heracell Vios 160i / 250i CR Thermo Scientific...

  • Page 84: Figure 5-4. "Max" Fill Level Indicator

    16. The CO control supplies the set amount of CO 17. If the auto-start routine has been completed, the progress indicator is hidden, and the main menu is displayed. The device is now operational. Heracell Vios 160i / 250i CR Thermo Scientific...
  • Page 85 70% max. Bulky objects in the workspace that dissipate heat may impair heat distribution. Large objects in the work space that dissipate heat may impair heat distribution. Heracell Vios 160i / 250i CR Thermo Scientific...
  • Page 86 Operation Water Filling Heracell Vios 160i / 250i CR Thermo Scientific...

  • Page 87: Chapter 6 Handling And Control

    “Auto-Start Function” page 6-12 • “Launching Steri-Run” page 6-16 • “User Configuration” page 6-17 • “Scaling the Trend Display” page 6-41 • “Error Messages” page 6-43 • “Measures After Power Outage” page 6-45 Heracell Vios 160i / 250i CR Thermo Scientific...

  • Page 88: Power Switch

    • The control loop sensors pass the heat-up phase (“Heat-up Phase of the Control Loop Sensors” page 6-7). • To turn the device off: Press the power switch; the switch illumination goes off. Heracell Vios 160i / 250i CR Thermo Scientific...

  • Page 89: Operating Panel And Operating Structure

    “Icon Description” page 6-39), • Steri-Run key, • Menu key, • Auto-start key. Note Extended pressure-sensitive area: To accept a failure message, the entire touchscreen can be used as a pressure-sensitive area. Heracell Vios 160i / 250i CR Thermo Scientific...

  • Page 90: Versions Without O2/N2 Control

    “Icon Description” page 6-39) 4. Key for starting the Steri-Run sterilization routine 5. Key for opening the menu navigation 6. Key for starting the auto-start routine 7. Display field water level RH Heracell Vios 160i / 250i CR Thermo Scientific...

  • Page 91: Structure Of The Operating Levels

    • A: Direct access to the control loop settings: Temperature, CO setpoint, • B: Starting the Steri-Run or auto-start routine of the unit, • C: Navigation through the submenus for device configuration. Heracell Vios 160i / 250i CR Thermo Scientific...

  • Page 92: Figure 6-5. Menu Structure Overview

    Handling and Control Operating Panel and Operating Structure Figure 6-5. Menu Structure Overview Heracell Vios 160i / 250i CR Thermo Scientific...

  • Page 93: Factory Presettings Of The Ican™ Touchscreen Controls

    After the heat-up phase has been completed, the control loop actual values are indicated. Note CO gas supply: During the 5-minute heat-up phase of the O control loop, the work space is not exposed to CO gas and the CO control and is disabled. Heracell Vios 160i / 250i CR Thermo Scientific...

  • Page 94: Behavior Of Keys During Adjustments

    Figure 6-7. Temperature Display Field and Temperature Selection Menu 2. To exit the Temperature menu: • Press the End key. 3. To set the temperature set value: • Press the Set value key. Heracell Vios 160i / 250i CR Thermo Scientific...

  • Page 95: Setting The Co2 Set Value

    Set Value 1. Press the CO display key. • The CO menu is displayed. Figure 6-9. CO Display and CO Menu 2. To exit the CO menu: • Press the End key. Heracell Vios 160i / 250i CR Thermo Scientific...

  • Page 96: Setting The O2 Set Value

    Setting the O Set Value This setting is possible only on versions with the optional O control. 1. Press the O display key. • The O menu is displayed. Heracell Vios 160i / 250i CR 6-10 Thermo Scientific...

  • Page 97: Figure 6-11. O2 Display And O2 Menu

    • Press the Enter key. • The system returns to the main menu. The O display shows the actual value currently measured in the workspace. Figure 6-12. Setting the O Set Value Heracell Vios 160i / 250i CR Thermo Scientific 6-11...

  • Page 98: Auto-Start Function

    When temperature and relative humidity have reached constant values, the measuring system is automatically adjusted to these values, and the workspace is supplied with the preset quantity of CO Heracell Vios 160i / 250i CR 6-12 Thermo Scientific...
  • Page 99 • Calibration values too high or too low, • No communication with sensor. Gas supply CO control loop: • No communication with sensor. In this case, the auto-start key is dimmed and its function is not available. Heracell Vios 160i / 250i CR Thermo Scientific 6-13...

  • Page 100: Activating The Auto-Start Routine

    3. Refill water, if necessary. 4. Activate the auto-start routine: • Press the Start key. 5. To air the workspace, open both device doors. When the audible alarm sounds after 30 seconds. Heracell Vios 160i / 250i CR 6-14 Thermo Scientific...

  • Page 101: Interrupting The Auto-Start Routine

    • The system returns to the status display, and the auto-start routine is resumed. 2. To cancel the auto-start routine: • Press the End key. • The warning triangle is displayed as a failure message together with the audible signal. Heracell Vios 160i / 250i CR Thermo Scientific 6-15...

  • Page 102: Launching Steri-Run

    An in-depth description of the function is given in the chapter Cleaning and disinfection (Chapter 8‚ page Heracell Vios 160i / 250i CR 6-16 Thermo Scientific...

  • Page 103: User Configuration

    • Changing the keypad lock code, • Date/time setting, • Adjusting display brightness, • Key tone setting, • Interface configuration, • User display screen language setting, • Reminder interval setting. Heracell Vios 160i / 250i CR Thermo Scientific 6-17...

  • Page 104: Figure 6-18. Settings/Setup Menu

    Figure 6-19. Changing the Keypad Lock Code 3. To enter the default 0000: • Press the corresponding numeric keys. • The number combination is displayed as encrypted text in the input box. Heracell Vios 160i / 250i CR 6-18 Thermo Scientific...

  • Page 105: Figure 6-20. Changing The Keypad Lock Code

    The input dialog allows date and time to be set to the required time zone. 1. Press the Menu key. 2. Select the menu command Date / Time. • The selection dialog shown in Figure 6-21 is displayed. Heracell Vios 160i / 250i CR Thermo Scientific 6-19...

  • Page 106: Figure 6-21. Date/Time Menu

    • Press the Enter key. 7. The system returns to the Date/Time menu. 8. For changing the time, choose the Time option. • The input dialog shown in Figure 6-23 is displayed. Heracell Vios 160i / 250i CR 6-20 Thermo Scientific...

  • Page 107: Figure 6-23. Setting The Time

    Setting the Contrast 1. Press the Menu key. 2. Select the menu command Settings / Setup. 3. Select the option Display contrast. • The input dialog shown in Figure 6-24 is displayed. Heracell Vios 160i / 250i CR Thermo Scientific 6-21...

  • Page 108: Figure 6-24. Adjusting Display Brightness

    Adjusting Key Tone Volume 1. Press the Menu key. 2. Select the menu command Settings / Setup. 3. Select the option Key tones. • The input dialog shown in Figure 6-25 is displayed. Heracell Vios 160i / 250i CR 6-22 Thermo Scientific...

  • Page 109: Figure 6-25. Adjusting Key Tone Volume

    The baud rate can be changed within the defined baud rates (9,600, 19,200, 38,400, 57,600 baud). Setting the Baud Rate 1. Press the Menu key. 2. Select the menu command Settings / Setup. • The selection dialog shown in Figure 6-26 is displayed. Heracell Vios 160i / 250i CR Thermo Scientific 6-23...

  • Page 110: Figure 6-26. Interface Usb Menu

    7. To activate the new settings: • Browse back to the main menu. • Wait for approx. 10 seconds and perform a reset by switching the device off and on using the power switch. Heracell Vios 160i / 250i CR 6-24 Thermo Scientific...

  • Page 111: Figure 6-28. Language Setting

    5. The new language appears in the display. The New message indicates that the selection has been changed, but not saved yet. 6. To accept and save the selection: • Press the Enter key. • The system returns to the Settings/Setup menu. Heracell Vios 160i / 250i CR Thermo Scientific 6-25...

  • Page 112: Figure 6-29. Choose The Desired Reminder Interval Function

    Figure 6-29 is displayed. Figure 6-29. Choose the Desired Reminder Interval Function. 3. Select the appropriate menu option, for example Steri-Run. • The input dialog shown in Figure 6-29 is displayed. Heracell Vios 160i / 250i CR 6-26 Thermo Scientific...

  • Page 113: Data Logging

    The Event logging selection menu (Figure 6-31) allows for accessing dialog screens for logging and displaying events that occur during ongoing operation of the unit: • Event display • Recording interval, • Error table. Heracell Vios 160i / 250i CR Thermo Scientific 6-27...

  • Page 114: Figure 6-31. Event Logging Menu

    3. Select the menu command Event display. • The list shown in Figure 6-32 appears on the display. Figure 6-32. Event Display 1. To browse forward in the list: Heracell Vios 160i / 250i CR 6-28 Thermo Scientific...

  • Page 115: Figure 6-33. Logging Cycle Setting

    The setting controls the logging cycle in sections of seconds during which the control loop measured values are logged during the operation of the device and displayed by the trend display (“Scaling the Trend Display” page 6-41). Heracell Vios 160i / 250i CR Thermo Scientific 6-29...

  • Page 116: Figure 6-34. Displaying The Error Table

    • The selection dialog shown in Figure 6-31 is displayed. 3. Select the menu command Event display. • The list shown in Figure 6-34 appears on the display. Figure 6-34. Displaying the Error Table Heracell Vios 160i / 250i CR 6-30 Thermo Scientific...

  • Page 117: Options

    Wiring details for the alarm relay are described in the section“Connecting the Alarm Contact” page 4-18. 1. Press the Menu key. 2. Select the menu command Options. Heracell Vios 160i / 250i CR Thermo Scientific 6-31...

  • Page 118: Figure 6-36. Alarm Menu

    1. To toggle between two states: • Press the + key. • Press the - key. 2. To accept and save the change: • Press the Enter key. • The system returns to the Options menu. Heracell Vios 160i / 250i CR 6-32 Thermo Scientific...

  • Page 119: Figure 6-38. Low Humidity Setting

    The enabling/disabling of the Low humidity function is entered into the event list. Setting the Gas Tight Screen As the aperture cross-sections are smaller when accessing the samples, devices equipped with the optional gas-tight screen achieve shorter recovery times for the incubation parameters: Heracell Vios 160i / 250i CR Thermo Scientific 6-33...

  • Page 120: Figure 6-39. Setting The Gas Tight Screen

    1. To toggle between two options: • Press the + key. • Press the - key. 2. To accept and save the change: • Press the Enter key. • The system returns to the Options menu. Heracell Vios 160i / 250i CR 6-34 Thermo Scientific...

  • Page 121: Figure 6-40. Setting The Water Level Sensor

    • in addition to the visual error message and the switching of the alarm relay. The audible alarm can be permanently disabled. 1. Press the Menu key. 2. Select the menu command Options. Heracell Vios 160i / 250i CR Thermo Scientific 6-35...

  • Page 122: Figure 6-41. Setting The Alarm Relay

    2. Select the menu command Options. • The selection dialog shown in Figure 6-35 is displayed. 3. Select the menu command O • The input dialog shown in Figure 6-42 is displayed. Heracell Vios 160i / 250i CR 6-36 Thermo Scientific...

  • Page 123: Figure 6-42. Switching The O2 Control On And Off

    In this case, the O display shows the actual value. Airing the Workspace If the unit is operated with an O or N supply, the workspace must be vented after de-activating O control. Heracell Vios 160i / 250i CR Thermo Scientific 6-37...

  • Page 124: Figure 6-43. Hepa Configuration

    • The input dialog shown in Figure 6-44 is displayed. Figure 6-44. Activating / De-Activating the HEPA Filter 1. To toggle between two states: • Press the + key. • Press the - key. Heracell Vios 160i / 250i CR 6-38 Thermo Scientific...

  • Page 125: Icon Description

    • The system returns to the User configuration menu. Function of the Individual Icons: Gas cylinder empty: Inactive. Overtemperature: This error indicator shows that the device control has activated the overtemperature protection and has switched to backup control. Heracell Vios 160i / 250i CR Thermo Scientific 6-39...

  • Page 126: Enabling/Disabling The Keypad Lock

    6-18). Resetting the code: If the keypad lock code is no longer available, the code must be reset to the standard code by the Technical Support of Thermo Fisher Scientific. Heracell Vios 160i / 250i CR 6-40 Thermo Scientific...

  • Page 127: Software Versions

    The trend display of the three control loops: • temperature, • 0...20% CO • 0...100% O (optional), can be scaled to two different versions. 1. Full screen display • Press the CO display field in the main menu. Heracell Vios 160i / 250i CR Thermo Scientific 6-41...

  • Page 128: Figure 6-48. Viewing The Co2 Concentration Graph Display

    (max. 30 data logger elements, equivalent to 30 minutes of logging operation at a storage rate of 60 seconds) is attained. • During zoom mode the trend display can be scrolled forward and back. Heracell Vios 160i / 250i CR 6-42 Thermo Scientific...

  • Page 129: Error Messages

    • The Error dialog box appears and the detected error is displayed. • The audible alarm is switched off. 2. To exit the error display: • Press the End key. • The error message disappears. Heracell Vios 160i / 250i CR Thermo Scientific 6-43...

  • Page 130: Resetting Overtemperature Protection

    1. Displaying the cause of an error: • Press anywhere on the touchscreen. • The Error dialog box appears and the detected error is displayed. • The audible alarm is switched off. Heracell Vios 160i / 250i CR 6-44 Thermo Scientific...

  • Page 131: Measures After Power Outage

    Alternatively, or when the above mentioned measures were not effective, start a sterilization run at 180 °C. Refer to Chapter “Steri-Run Sterilization Routine” page 8-7. The sterilization routine can be interrupted after approx. 1 hour. The sensors should then have dried. Heracell Vios 160i / 250i CR Thermo Scientific 6-45...

  • Page 132: Troubleshooting

    The error tables indicate source of error, cause of error, and possible corrections. For any communication with the Technical Service of Thermo Fisher Scientific, please have the device model number and serial number ready. Heracell Vios 160i / 250i CR 6-46 Thermo Scientific...
  • Page 133 The fan does not provide full or any power IR sensor changed New serial number Acknowledge the detected alarm Heracell Vios 160i / 250i CR Thermo Scientific 6-47...
  • Page 134 Reset unit. If the error plausible temperature signal occurs repeatedly, call field service. Calibration values too Max. adjust value for Acknowledge alarm, high/low temperature exceeded / enter different tar- not attained get value. Heracell Vios 160i / 250i CR 6-48 Thermo Scientific...
  • Page 135 After Power Outage” page 6-45. Gas cylinder A empty Gas cylinder A is empty Replace gas cylinder A Gas cylinder B empty Gas cylinder B is empty Replace gas cylinder B Heracell Vios 160i / 250i CR Thermo Scientific 6-49...
  • Page 136 The LED on the Fan in the rear wall outside lights up red enclosure doesn‘t work properly This error appears on the display only; it is not entered in the error log. Heracell Vios 160i / 250i CR 6-50 Thermo Scientific...
  • Page 137  - 45 min. after a door was opened,  - 159 min. after a setpoint change. This setting can be changed by field service. Heracell Vios 160i / 250i CR Thermo Scientific 6-51...
  • Page 138 Handling and Control Error Messages Heracell Vios 160i / 250i CR 6-52 Thermo Scientific...

  • Page 139: Chapter 7 Shut-Down

    4. The water reservoir starts draining. Figure 7-1. Fill and Drain Valve of Water Reservoir 5. Allow the water reservoir to drain completely into the collecting vessel. 6. Disconnect the hose from the quick connect valve. Heracell Vios 160i / 250i CR Thermo Scientific...
  • Page 140 11. Disconnect the gas pressure hoses from the sleeve at the rear of the device. 12. Until the device is completely cooled, the workspace must be continuously ventilated. Leave the glass door and the outer door open and secure them in this state. Heracell Vios 160i / 250i CR Thermo Scientific...

  • Page 141: Chapter 8 Cleaning And Disinfection

    1. Thoroughly remove dirt residues and deposits using a solution of tepid water and dishwashing soap. 2. Wipe the surfaces clean using a clean cloth and clear water. 3. Then, wipe the surfaces dry using a clean cloth. Heracell Vios 160i / 250i CR Thermo Scientific...

  • Page 142: Disinfection Procedures

    8-2) out of its seat on the water reservoir cover panel and remove it. 8. Remove the HEPA filter (2/Figure 8-2) out of the airbox (1/Figure 8-2) and reinstall the empty airbox. Heracell Vios 160i / 250i CR Thermo Scientific...

  • Page 143: Wipe/Spray Disinfection Of The Workspace

    Prior to cleaning and disinfection work, disconnect the device from the power supply! • Turn the device off using the power switch. • Unplug the power connector and protect it against accidental reconnection. • Make sure the device is de-energized. Heracell Vios 160i / 250i CR Thermo Scientific...
  • Page 144 2. Remove the airbox and the HEPA filter. The installation and removal of the airbox and the HEPA filter are described in section “Replacing the HEPA Filter in the Workspace” page 9-7. Heracell Vios 160i / 250i CR Thermo Scientific...

  • Page 145: Figure 8-2. Hepa Filter And Airbox

    4. Separate the top part from the rear part of the air duct (2/Figure 8-3) and remove it from the workspace. Heracell Vios 160i / 250i CR Thermo Scientific...

  • Page 146: Figure 8-3. Air Duct

    1. Spray the surfaces of the workspace, the shelving system and parts removed in previous steps with 70% ethanol one more time. 2. Allow the 70% ethanol to air dry. 3. Reinstall the shelf system and the removed components. Heracell Vios 160i / 250i CR Thermo Scientific...

  • Page 147: Steri-Run Sterilization Routine

    The germ reduction efficiency achieved is equal to 10 (12-log reduction) to the ISO 11138 standard. On request, Thermo Scientific will supply information on the pertinent tests. The electromechanical door lock provides Steri-Run with an extra degree of protection against the risk of burns.

  • Page 148: Progress Of A Steri-Run Sterilization Routine

    During the Steri-Run sterilization routine, the workspace is heated up to 180 °C. Make sure that: • all cultures are removed from the workspace, • any added accessories are removed from the workspace. Heracell Vios 160i / 250i CR Thermo Scientific...

  • Page 149: Figure 8-4. Steri-Run Sterilization Routine Phases

    The device cools down until the originally set temperature set value is reached. The door lock is released automatically when the temperature in the workspace drops below 65°C. Temp. Warming up Sterilization Cooling down Time Start Figure 8-4. Steri-Run Sterilization Routine Phases Heracell Vios 160i / 250i CR Thermo Scientific...

  • Page 150: Activate Steri-Run

    65°C. • While the Steri-Run sterilization routine is running, the display shows the current status and outputs the following information: • temperature, • start time, • phase, Heracell Vios 160i / 250i CR 8-10 Thermo Scientific...

  • Page 151: Canceling Steri-Run

    • The system returns to the status display, and the sterilization routine is resumed. 4. To interrupt Steri-Run from the status display: • Press the Stop key. • As a safety scan, the Steri-Run - Stop dialog window appears. Proceed with work step 2. Heracell Vios 160i / 250i CR Thermo Scientific 8-11...

  • Page 152: Steri-Run Interruption Due To Error

    • Press the End key. • The error message is displayed. • If the error message is confirmed, the system returns to the main menu. Figure 8-7. Interrupting / Canceling Steri-Run Heracell Vios 160i / 250i CR 8-12 Thermo Scientific...

  • Page 153: Completing Steri-Run

    If the door will be opened and closed again whilst Steri-Run sterilization is running, the routine returns to a phase which will ensure a continuation without faults. Note  Risk of burns! Avoid opening the door unless in case of an emergency. Heracell Vios 160i / 250i CR Thermo Scientific 8-13...

  • Page 154: Opening The Door After Canceling Steri-Run

    Steri-Run sterilization routine. During the routine run or immediately after completion of the run, always wear safety gloves when touching these surfaces! Heracell Vios 160i / 250i CR 8-14 Thermo Scientific...

  • Page 155: Chapter 9 Maintenance

    61010-1, IEC 61010-2-010) Note Functional check: If safety devices were removed or disabled for inspections, the device must not be operated before the safety devices have been reinstalled and checked for their correct function. Heracell Vios 160i / 250i CR Thermo Scientific...

  • Page 156: Service Intervals

    • Perform Technical Service service inspection. Note Service contract: Thermo Scientific offer a device-specific service contract that comprises all test and service works required. Preparing Temperature Calibration To determine the exact measured value of the incubator’s integral temperature sensor, a temperature comparison measurement must be performed every three months.

  • Page 157: Temperature Calibration Procedure

    6. Temperature Calibration Procedure Temperature Calibration Procedure Measurement example: • Temperature set value: 37 °C Reference temperature: 36.4 °C 1. Press the Temperature Display key. • The Temperature menu (Figure 9-2) is displayed. Heracell Vios 160i / 250i CR Thermo Scientific...

  • Page 158: Figure 9-2. Temperature Display Field And Temperature Selection Menu

    • Press the + key. To reduce the set value: • Press the - key. 5. Accept and save the target value: • Press the Enter key. • Press the Save key. Heracell Vios 160i / 250i CR Thermo Scientific...

  • Page 159: Preparing The Co2 Calibration

    The measuring sample is withdrawn through the measurement port [1/Figure 9-4] of the glass door. The comparison measurement must be performed when the device is completely heated to the set temperature. Figure 9-4. Gas-Tight Screen Measurement Port Heracell Vios 160i / 250i CR Thermo Scientific...

  • Page 160: Co2 Calibration Procedure

    1. Press the CO display key. • The CO menu is displayed. 2. To exit the CO menu: • Press the End key. 3. To enter the Calibration submenu: • Press the Calibration key. Heracell Vios 160i / 250i CR Thermo Scientific...

  • Page 161: Replacing The Hepa Filter In The Workspace

    Steps for replacing the HEPA filter: 1. Power down the unit, shut off the gas supply and allow the workspace to vent. 2. Remove the center and bottom shelves out of the workspace. Heracell Vios 160i / 250i CR Thermo Scientific...

  • Page 162: Figure 9-6. Removing The Airbox

    9-7)on the left-hand side from the HEPA filter tabs (6/Figure 9-7). 7. Unlatch the tabs on the right-hand side (3/Figure 9-7) of the airbox (1/Figure 9-7) from the corresponding slots provided in the HEPA filter (4/Figure 9-7). Heracell Vios 160i / 250i CR Thermo Scientific...

  • Page 163: Figure 9-7. Installing The Hepa Filter

    “Reminder Interval Setting” page 6-26. The reminder interval for HEPA filter replacement may be set between 1 and 12 months. We recommend setting this parameter to the factory preset value of 12 months. Heracell Vios 160i / 250i CR Thermo Scientific...

  • Page 164: Replacing The Hepa Filter In The Rear Wall Filter Enclosure

    1. Make sure that the gas supply is shut off. 2. Loosen the hose clamp (3/Figure 9-8). 3. Remove the gas hose (4/Figure 9-8) from the gas inlet filter sleeve. Procedure for all gas inlet filters: Heracell Vios 160i / 250i CR 9-10 Thermo Scientific...

  • Page 165: Device Fuse Replacement

    When changing the door seal of a device, make sure that a door seal is used for the electromagnetic door lock. Note  The glass door seal cannot be replaced by the customer. Contact service. Heracell Vios 160i / 250i CR Thermo Scientific 9-11...

  • Page 166: Spare Parts And Accessories

    CAUTION  Observe the instructions or technical data of the manuals delivered with the spare parts or accessories. The instructions or technical data can differ from those stated in this manual. Heracell Vios 160i / 250i CR 9-12 Thermo Scientific...

  • Page 167: Chapter 10 Waste Disposal

    Components mounted on circuit boards containing epoxy resin bonder. Plastic components, general ABS and PPS GF40, note material identification. Outer Housing Stainless steel 1.4307, brushed. Device backpanel Stainless steel 1.4307, brushed. Heracell Vios 160i / 250i CR Thermo Scientific 10-1...
  • Page 168 GF/PTFE membrane, Prefilter, stainless steel 1.4401 wire mesh. Air inlet filter Packaging Corrugated cardboard, polyethylene foil, styrofoam shaped elements and polypropylene Door seal magnet Permanent magnet Cell Locker Chamber Polycarbonate Makrolon 2528 Heracell Vios 160i / 250i CR 10-2 Thermo Scientific...

  • Page 169: Chapter 11 Specifications

    Specifications Table of Contents • “Heracell Vios 160i CR” page 11-2 • “Heracell Vios 250i CR” page 11-6 Heracell Vios 160i / 250i CR Thermo Scientific 11-1...

  • Page 170: Heracell Vios 160I Cr

    Specifications Heracell Vios 160i CR Heracell Vios 160i CR Name Unit Value Mechanical Outer dimensions (W x H x D) 637 x 901 x 881 Inner dimensions (W x H x D) 470 x 607 x 576 Inner space volume,...
  • Page 171 Specifications Heracell Vios 160i CR Name Unit Value Constant humidity at 37 °C (high humidity mode) % rH approx. 93 Constant humidity at 37 °C (low humidity mode) % rH approx. 90 Others Sound pressure level (DIN 45 635, part 1) dB(A) <...
  • Page 172 Specifications Heracell Vios 160i CR Heracell Vios 160i CR Name Unit Value gas supply system Gas purity min. 99.5 or med. quality Primary pressure min. 0.8 - max. 1 (11.6-14.5 psi) Measurement and control range Vol -% 0...20 Control deviation over time Vol -% ±...
  • Page 173 Specifications Heracell Vios 160i CR Name Unit Value Circuit breaker 16 A 230 V:  Rated power Sterilization: 1.10  Incubation: 0.56 220 V:  Sterilization: 0.97 Incubation: 0.51 120 V:  Sterilization: 1.01 Incubation: 0.55 100 V:  Sterilization: 0.72...

  • Page 174: Heracell Vios 250I Cr

    0.07 during Steri-Run sterilization kWh/h 0.75 Humidity Water quality (sterile distilled) Electrical resistance: 50 K-Ohm-cm to 1 M-Ohm-cm Conductivity: 1 to 20 S/cm Capacities: max. 3 / min 0.5 Incubation operation Heracell Vios 160i / 250i CR 11-6 Thermo Scientific...
  • Page 175 % rH max. 80 Location elevation m above sea max. 2000 level determined on the basis of DIN 12880 for devices with standard feature set. See calibration instructions for detailed information. Heracell Vios 160i / 250i CR Thermo Scientific 11-7...
  • Page 176 Pollution severity (EN 61010) 230 V:  Rated current Sterilization: 5.5 Incubation: 3.3 220 V:  Sterilization: 5.3 Incubation: 3.2 120 V:  Sterilization: 10.4 Incubation: 6.3 100 V:  Sterilization: 8.9 Incubation: 5.3 Heracell Vios 160i / 250i CR 11-8 Thermo Scientific...
  • Page 177 230 V:  Rated power Sterilization: 1.26 Incubation: 0.76 220 V:  Sterilization: 1.16 Incubation: 0.69 120 V:  Sterilization: 1.25 Incubation: 0.75 100 V:  Sterilization: 0.89  Incubation: 0.53 EMV class Heracell Vios 160i / 250i CR Thermo Scientific 11-9...
  • Page 178 Specifications Heracell Vios 250i CR Heracell Vios 160i / 250i CR 11-10 Thermo Scientific...

  • Page 179: Chapter 12 Data Communication

    The assigned COM port can be located in the Windows Device Manager/Ports dialog box, e.g. USB Serial Port (COM5), and is then defined as communication port in the Heracell Vios 160i / program (see “Program Heracell Vios 160i / 250i CR” page 12-21). 250i CR Heracell Vios 160i / 250i CR Thermo Scientific 12-1...

  • Page 180: Installing The Usb Port Driver

    As soon as the Windows Hardware Detector has identified the USB port, the Find New Hardware Wizard dialog box opens. 1. Choose the INSTALL FROM A LIST OR SPECIFIC LOCATION (ADVANCED) option. Heracell Vios 160i / 250i CR 12-2 Thermo Scientific...
  • Page 181 4. On the data CD, select the DRIVER sub-directory. 5. The installation routine installs the driver: EVAL22 Board USB. After the installation has been completed successfully, the routine is completed with FINISH. Heracell Vios 160i / 250i CR Thermo Scientific 12-3...

  • Page 182: Structure Of The Command Sequences For Data Communication

    =amount of payload in this telegram (00 – ff ) cc = checksum: CRC8-CCITT: x8 + x2 + x1 + 1 = 0x07  without cc and <CR> XXXX = bb-bytes user data Response with an error message: Response: !:aaaa:bb:XX:cc<CR> Heracell Vios 160i / 250i CR 12-4 Thermo Scientific...

  • Page 183: Overview Of General Parameters (Addresses 0Xxx)

    Overview of Incubator Parameters (Addresses 2xxx) Incubator parameters are divided into: • the basic parameters of the three control loops temperature, CO and O • the internal function parameters of operating functions and data logging. Heracell Vios 160i / 250i CR Thermo Scientific 12-5...

  • Page 184: Reading Basic Parameters

    Low humidity indicator (1 active, 0 2 bytes / hexadecimal inactive) with the format xx Example of unit status and (error) status control loops (for details see error messages) All values have two decimal places. Heracell Vios 160i / 250i CR 12-6 Thermo Scientific...

  • Page 185: Reading Internal Function Parameters

    *6) Send the next 7 entries. Set reading pointer automatically to next new entry, read up to 7 entries max. *7) Re-send entries of last telegram. Can be used after communication error has occurred. Heracell Vios 160i / 250i CR Thermo Scientific 12-7...

  • Page 186: Error Memory Structure

    Therefore, a response consists of 1+(10 x 2) = 21 data bytes plus separator. A data set always delivers the date, the time, the faulty control loop, the device status, and the error message. Heracell Vios 160i / 250i CR 12-8 Thermo Scientific...

  • Page 187: Error Memory Data Set Structure Scheme

    Therefore, the following information is transmitted in this data set: • Created on 11 January 2006 at 15:55:34 hrs. • A device error has occurred and the set temperature is too high. Heracell Vios 160i / 250i CR Thermo Scientific 12-9...

  • Page 188: Overview Of The Possible Error Messages In Hex Coding

    Sensor breakage 0x0002 Actual value high 0x0004 Actual value low 0x0008 Actual value not plausible 0x0010 Calibration values too high/low 2 control loop error status 0x0001 Sensor breakage 0x0002 Actual value high Heracell Vios 160i / 250i CR 12-10 Thermo Scientific...

  • Page 189: O2 Control Loop And Water Level

    Set the data logger pointer to the oldest entry and output the first data sets. Query: ?:2401:00::cc<CR> Output of the following data sets, the reading pointer automatically moves  gradually from the older entries to the current entries. Heracell Vios 160i / 250i CR Thermo Scientific 12-11...
  • Page 190 !:2400:e0:010b01060f3700000177002800d40000110b01060f3800000172003200d20352..:80 First data set !:2400:e0:010b01060f3700000177002800d4000011 (consisting of 32 byte ASCII characters) Second data set 0b01060f3800000172003200d20352..:80 (beginning of the second data set after 32 bytes of the first data set) Heracell Vios 160i / 250i CR 12-12 Thermo Scientific...

  • Page 191: Data Logger Data Set Structure Scheme

    • the temperature is 37.5°C, • gas concentration 4.0% CO , 21.2% O Note Example of a code: For an example of a code, please refer to the end of this chapter. Heracell Vios 160i / 250i CR Thermo Scientific 12-13...

  • Page 192: Overview Of Possible Event Entries In Bit Coding Overview Of Event Entries, Part I

     value, new O (2 bytes each) 0x50 Start auto-start Status / error registration for temperature, CO and rH 0x51 auto-start successfully completed Current actual values for temperature, CO and rH Heracell Vios 160i / 250i CR 12-14 Thermo Scientific...

  • Page 193: Examples Of Data Logger Codes

    Byte 2: day of entry  Byte 3: month Byte 4: year Byte 5: hour Byte 6: minutes Byte 7 and 8: device status  Byte 9 to 16: various data on the event Heracell Vios 160i / 250i CR Thermo Scientific 12-15...

  • Page 194: Functions For Data Logger Queries

    // calculate checksum: inverted XOR of all bytes // without checksum and <CR> for (i = 0; i < 11; i ++) bcc = (bcc^string[i]); // copy checksum string[11] = hexa(bcc/16); string[12] = hexa(bcc%16); Heracell Vios 160i / 250i CR 12-16 Thermo Scientific...
  • Page 195 = 0; // Negative number?  if (z < 0) { // set algebraic sign b[0] = ‘-’;l = 1; // convert value z = 0xffffffff-z+1; } // store decimal place Heracell Vios 160i / 250i CR Thermo Scientific 12-17...
  • Page 196 // set data logger to beginning and read if (!GetTele) { send_telegramm („2400“); } else{ // read additional data sets send_telegramm („2401“); } len = get_telegramm (buffer); // no telegram received Heracell Vios 160i / 250i CR 12-18 Thermo Scientific...
  • Page 197 (status & INFO_ERROR){ str_cpy (&excelstring[len], „Error active;“, 13);  len += 13; } else{ // query all device errors (see “Overview of Possible Event Entries in Bit Coding Overview of Event Heracell Vios 160i / 250i CR Thermo Scientific 12-19...
  • Page 198 += num_2_str (SollO2, &excelstring[len]); excelstring[len ++] = ‘;’; len += num_2_str (SollrH, &excelstring[len]); excelstring[len ++] = ‘;’; excelstring[len] = ‘\n’;  len += 1;  WriteFile (FileHandle, excelstring, len); break; Heracell Vios 160i / 250i CR 12-20 Thermo Scientific...

  • Page 199: Program Heracell Vios 160I / 250I Cr

    0; Program Heracell Vios 160i / 250i CR The program provides a user surface (only with English menu designation) for handling the data communication between a device and a connected computer. Heracell Vios 160i / 250i CR Thermo Scientific 12-21...

  • Page 200: Installing The Heracell Vios 160I Cr & Heracell Vios 250I Cr

    • Creating a service file (servicefile) to be sent to the Technical Service of Thermo Fisher Scientific. The information of the service file is very useful for systematic troubleshooting. The data sets are stored in the proprietary format *.SRF. Installing the Heracell Vios 160i CR Heracell Vios 250i CR & 1. Starting the installation routine: •...
  • Page 201 Data Communication Program Heracell Vios 160i / 250i CR Operating the Heracell Vios 160i CR Heracell Vios 250i CR & User menu structure: The user surface is divided into two main menus: • MAIN with two functional elements: • Program version output: FIRMWARE VERSION •...
  • Page 202 4. Save the settings (in an INI file): • Press the SAVE TO FILE key. Note Transmission speeds: The transmission speed settings in the user menu PRESETTING and of the device must be identical! Heracell Vios 160i / 250i CR 12-24 Thermo Scientific...
  • Page 203 • If the incubator returns a response string, the communication connection to the incubator has been established. • If a connection cannot be established, an error dialog is displayed: • 3. Exit the error dialog: • Press the OK key. Heracell Vios 160i / 250i CR Thermo Scientific 12-25...
  • Page 204 • Saving data sets as files: • Press the SAVE TO FILE key. DATA LOGGER The submenu DATA LOGGER is used for reading the event entries into the text box of the user surface. Heracell Vios 160i / 250i CR 12-26 Thermo Scientific...
  • Page 205 • Define file name and saving directory in the Windows dialog box. 2. Start saving process: • Press the OK key. Note Duration of the procedure: The compilation of device informations and the creation of the service file may take some time. Heracell Vios 160i / 250i CR Thermo Scientific 12-27...
  • Page 206 Data Communication Program Heracell Vios 160i / 250i CR PASSWORD The submenu PASSWORD can only be accessed by the service personnel of Thermo Fisher Scientific. Heracell Vios 160i / 250i CR 12-28 Thermo Scientific...

  • Page 207: Chapter 13 Device Log

    Device Log Please list carried out works here: Device type: Part No.: Serial number: Service number: Location: Operator’s note: Work carried out: Comments: Date Signature Heracell Vios 160i / 250i CR Thermo Scientific 13-1...
  • Page 208 Device Log Heracell Vios 160i / 250i CR 13-2 Thermo Scientific...

  • Page 209: Chapter 14 Contact Information Thermo Scientific

    Contact Information Thermo Scientific Overview of the Thermo Fisher Scientific international sales organisations  postal address Germany: Thermo Electron LED GmbH Robert-Bosch-Straße 1 D - 63505 Langenselbold Enquiries from Germany: Phone Sales 0800 1 536376 Phone Service 0800 1 112110...
  • Page 210 Contact Information Thermo Scientific Enquiries at address USA: Thermo Fisher Scientific 275 Aiken Road Asheville, NC 28804 Enquiries from USA/Canada Sales: +1 866 984 3766 Service+1 800 438 4851 Enquiries from Latin America Sales: +1 866 984 3766 Service:+1 866 984 3766 Enquiries from Asia:...
  • Page 211 Contact Information Thermo Scientific Germany: Postal Address Germany: Thermo Electron LED GmbH Robert-Bosch-Straße 1 D - 63505 Langenselbold Phone Sales Toll free 0800 1 536 376 or +49 6184 90 6940 Service Toll free 0800 1 112110 or +49 6184 90 6940...
  • Page 212 Contact Information Thermo Scientific Heracell Vios 160i / 250i CR 14-4 Thermo Scientific...

  • Page 213: Appendix A Sixfold Gas Tight Screen For Cell Locker System

    Sixfold gas tight screen for C ELL LOCKER ELL LOCKER Note Performance Change Due to the design of the sixfold gas tight screen the performance of the incubator changes, see Technical Data). Heracell Vios 160i / 250i CR Thermo Scientific...

  • Page 214: Installation Of The Shelving

    Before the perforated shelf with attached guiding rails can be installed, the guiding rails (2) must be attached to the shelf. The guiding rails can be removed, if necessary for cleaning. Place the perforated shelf with the attached guiding rails (1) and middle openings in the lower position. Heracell Vios 160i / 250i CR Thermo Scientific...
  • Page 215 Place the perforated shelf so it snaps in behind the rail. The metal tab underneath the shelf needs to be fixed behind the rail for door closure. Close the sixfold gas tight screen. Heracell Vios 160i / 250i CR Thermo Scientific...
  • Page 216 Sixfold Gas Tight Screen for Cell Locker System Installation of the Shelving Heracell Vios 160i / 250i CR Thermo Scientific...

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