Setting The Sterilizing Temperature - Thermo Scientific VARIOKLAV 25T Operating Manual

The thermolock is activated. After sterilization, pressure is reduced step by step in
very small increments. There is no danger of overboiling. The vessels will cool in a
controlled manner. The sterilizing chamber is pre-aerated between 100 °C and 90
°C. However, no vacuum is created, so that the culture media will not boil in this
temperature range. The liquid loss by evaporation is thus reduced considerably.
After cooling to a reference temperature (if the media temperature control is
present: media temperature) of 90 °C, the program cycle is completed.
With Type RM fast recooling system expansion option:
Liquids RM
For minimizing batch times when sterilizing liquids or culture media in open or
slightly closed vessels.
Cooling water flows through the sterilizing chamber in a separate circuit. The
sterilized items are quickly and carefully cooled to a reference temperature (if
media temperature control is present: media temperature) of 90 °C.
6.2

Setting the sterilizing temperature

The selected set temperature (in °C) is shown on the cycle display (16).
The total available temperature range will depend on the operating data of your
sterilizer. The following range is valid for Germany
Type 25T:
We recommend the following set temperatures for sterilization and disinfection:
98 °C
103 °C
121 °C
134 °C
You can set the set temperature on the control panel (5):
♦ Press the selected program key (18) repeatedly until the sterilizing
♦ Increase or decrease the sterilizing temperature using the ▲ key (14) or
The set temperature will increase or decrease in one-degree increments
with each press of the ▲ key (14) or the ▼ key (15).
6) *
In other countries other temperature ranges for setting may be available.
03/2007
for disinfection; for steaming and dissolving agar-agar
for thermally instable media
for culture media, solutions and other liquids, as well as solids
such as glassware or instruments
for destructive sterilization
temperature appears in the cycle display (Figure 7).
Steril. Temp.
Figure 7: Sterilizing temperature display
the ▼ key (15).
6 Programs
6))
:
98 °C to 135 °C.
Cycle
121 C
°
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