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Thermo Scientific HERACELL VIOS 160i LK User Manual

Thermo Scientific HERACELL VIOS 160i LK User Manual

Co2 incubator
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Thermo Scientific
HERA
CELL VIOS
HERA
CELL VIOS
CO
User Manual
50162755 A
July 2022
160i LK/
250i LK
Incubator
2

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Summary of Contents for Thermo Scientific HERACELL VIOS 160i LK

  • Page 1 Thermo Scientific  HERA 160i LK/ CELL VIOS HERA 250i LK CELL VIOS Incubator  User Manual 50162755 A July 2022...
  • Page 2 HERACELL VIOS™, Steri-run™, Steri-cycle™, iCan™, THRIVE™ and Cell locker™ are registered trademarks of  Thermo Scientific. Thermo Scientific is a brand of Thermo Fisher Scientific Inc. All other trademarks mentioned in the operating instructions are the exclusive property of the respective ...

  • Page 3: Table Of Contents

    Chapter 1 Delivery of the Incubator ....................1-1 Packaging ............................1-1 Acceptance Inspection ........................1-1 Standard Equipment Components HERACELL VIOS 160i LK / 250i LK........1-2 Optional Equipment Components HERACELL VIOS 160i LK / 250i LK........1-2 Optional Equipment Components HERACELL VIOS 160i LK............1-3 Optional Equipment Components HERACELL VIOS 250i LK............
  • Page 4 Retrofitting/Modifications........................ 2-13 Chapter 3 Description of the Device ....................3-1 HERACELL VIOS 160i LK / VIOS 250i LK Front View ..............3-2 HERACELL VIOS 160i LK / VIOS 250i LK Rear View ..............3-4 Looped-through Gas Supply at HERACELL VIOS 160i LK / ...
  • Page 5 Software Versions ........................6-43 Trend Display Scaling........................6-43 Error Messages..........................6-45 Response to an Error Message Event .................... 6-45 Resetting Overtemperature Protection ..................6-46 Measures After Power Outage......................6-47 Troubleshooting .......................... 6-48 HERA 160i LK / 250i LK CELL VIOS Thermo Scientific...
  • Page 6 Replacing the Door Seal ........................9-10 Chapter 10 Waste Disposal....................... 10-1 Overview of the Materials Used: ....................... 10-1 Chapter 11 Specifications......................... 11-1 HERACELL VIOS 160i LK......................11-2 HERACELL VIOS 160i LK......................11-4 HERACELL VIOS 250i LK......................11-6 HERACELL VIOS 250i LK......................11-8 Chapter 12 Data Communication .....................
  • Page 7 Example of a Code for a a Data Logger Query ................12-16 Program HERACELL VIOS 160i LK / HERACELL VIOS 250i LK..........12-21 Installing the HERACELL VIOS 160i LK & HERACELL VIOS 250i LK ....... 12-22 Operating the HERACELL VIOS 160i LK & HERACELL VIOS 250i LK ......12-23 Chapter 13 Device Log ........................
  • Page 8 Table of Contents HERA 160i LK / 250i LK CELL VIOS Thermo Scientific...
  • Page 9 Figure 3-1. HERA CELL VIOS 160i LK / VIOS 250i LK Front View...........3-2 Figure 3-2. HERACELL VIOS 160i LK / VIOS 250i LK Rear View............3-4 Figure 3-3. Looped-through Gas Supply HERACELL VIOS 160i LK / HERACELL VIOS 250i LK ..3-5 Figure 3-4. HEPA-Filter and Airbox .......................3-8 Figure 3-5.
  • Page 10 Figure 6-20. Changing the Keypad Lock Code ..................6-19 Figure 6-21. Date/Time Menu......................6-20 Figure 6-22. Setting the Date ........................6-20 Figure 6-23. Setting the Time .......................6-21 Figure 6-24. Adjusting Display Brightness ....................6-22 Figure 6-25. Adjusting Key Tone Volume.....................6-23 HERA 160i LK / 250i LK CELL VIOS Thermo Scientific...
  • Page 11 Figure 8-9. Door Lock Latch and Emergency Release on Underside of Incubator ........ 8-13 Figure 9-1. Preparing Temperature Calibration..................9-3 Figure 9-2. Temperature Display Field and Temperature Selection Menu..........9-4 HERA 160i LK / 250i LK CELL VIOS Thermo Scientific...
  • Page 12 Figure 9-4. Gas-Tight Screen Measurement Opening ................9-6 Figure 9-5. CO2 Calibration Procedure ....................9-7 Figure 9-6. Removing the Airbox ......................9-8 Figure 9-7. Installing the HEPA-Filter ....................9-9 Figure 9-8. Gas Inlet Filter Installation ....................9-10 HERA 160i LK / 250i LK CELL VIOS Thermo Scientific...

  • Page 13: Chapter 0 Preface

    • which protective measures apply when specific agents are used, • which measures are to be taken in the case of an accident. • Repairs to the device must be carried out only by trained and authorized expert personnel. HERA 160i LK / 250i LK CELL VIOS Thermo Scientific...

  • Page 14: Applicability Of The Instructions

    Warranty Requirements for Warranty Thermo Scientific warrants the operational safety and functions of the CO2 incubator for 2 years, only under the condition that: • the device is operated and serviced exclusively in accordance with its intended purpose and as described in these operating instructions, •...

  • Page 15: Explanation Of Safety Information And Symbols

    CAUTION Indicates a hazardous situation which, if not avoided, could result in minor or moderate injuries. CAUTION Indicates a situation which, if not avoided, could result in property damage. Note Is used for applicational hints and useful information. HERA 160i LK / 250i LK CELL VIOS Thermo Scientific...

  • Page 16: Additional Symbols For Safety Information

    Preface Explanation of Safety Information and Symbols Additional Symbols for Safety Information: Wear safety gloves! Wear safety goggles! Harmful liquids! Electric shock! Hot surfaces! Fire hazard! Explosion hazard! Suffocation hazard! HERA 160i LK / 250i LK CELL VIOS Thermo Scientific...

  • Page 17: Symbols On The Device

    To that end, a controlled set of physiological ambient conditions is produced in the workspace of the device by exerting precise control of the following parameters: • Temperature • CO content • O content • Relative humidity HERA 160i LK / 250i LK CELL VIOS Thermo Scientific...

  • Page 18: Incorrect Use

    Part 1: General requirements • FCC 47, Part 15, § 15.107, EMC regulation FCC • FCC 47. Part 15, § 15.109, EMC regulation FCC For other countries, the applicable national regulations are binding. HERA 160i LK / 250i LK CELL VIOS Thermo Scientific...

  • Page 19: Safety Notes On Gases

    • measures to be taken in case of accidents or failures. These instructions must be repeated at appropriate intervals and must comprise the particular operating instructions of the gas supplier. HERA 160i LK / 250i LK CELL VIOS Thermo Scientific...

  • Page 20: Safety Notes On Carbon Dioxide (Co2)

    ) released into the room atmosphere may cause suffocation. If N released, initiate safety measures immediately! • Leave the room immediately and do not allow others to enter the room! • Inform security service or fire department! HERA 160i LK / 250i LK CELL VIOS Thermo Scientific...

  • Page 21: Chapter 1 Delivery Of The Incubator

    “Packaging“ page 1-1 • “Acceptance Inspection“ page 1-1 • “Standard Equipment Components HERACELL VIOS 160i LK / 250i LK“ page 1-2 • “Optional Equipment Components HERACELL VIOS 160i LK / 250i LK“ page 1-2 • “Optional Equipment Components HERACELL VIOS 250i LK“...
  • Page 22 Delivery of the Incubator Standard Equipment Components HERACELL VIOS 160i LK / 250i LK Standard Equipment Components HERA 160i LK / 250i LK CELL VIOS Quantity of components supplied Pieces Water reservoir cover panel Max. water level sensor Rear wall air duct...

  • Page 23: Optional Equipment Components Heracell Vios 160I Lk

    Delivery of the Incubator Optional Equipment Components HERACELL VIOS 160i LK Optional Equipment Components HERA 160i LK CELL VIOS Components Pieces Threefold gas-tight screen 6-fold gas tight screen Cell locker Water funnel Optional Equipment Components HERA 250i LK CELL VIOS...

  • Page 24: Optional Equipment Components Heracell Vios 250I Lk

    Delivery of the Incubator Optional Equipment Components HERACELL VIOS 250i LK HERA 160i LK / 250i LK CELL VIOS Thermo Scientific...

  • Page 25: Chapter 2 Installation Of The Device

    • No direct exposure to sunlight. • Devices that produce excessive heat must not be placed near the location of HERA 160i LK CELL VIOS / HERA 250i LK CELL VIOS HERA 160I LK / 250i LK CELL VIOS Thermo Scientific...

  • Page 26: Room Ventilation

    To ensure the accessibility for care and maintenance works, keep larger side and rear distances. The swivel-out for units with 6-fold gas tight screen with Cell locker must have a clearance to the wall directly at the hinge side of >= 31 cm / 12.2". HERA 160i LK / 250i LK CELL VIOS Thermo Scientific...

  • Page 27: Transport

    Before moving stacked devices, be sure to remove the support brackets! Stacked devices provided with a mobile base rack must only be moved inside rooms on even floors without any slopes. HERA 160i LK / 250i LK CELL VIOS Thermo Scientific...

  • Page 28: Figure 2-3. Stacking

    1. Place the lower unit with the device stands (3/Figure 2-3) on the stacking elements (1/Figure 2-4) on top of the mobile base rack (2/Figure 2-4) or the support frame without castors (4/Figure 2-3). HERA 160i LK / 250i LK CELL VIOS Thermo Scientific...

  • Page 29: Figure 2-4. Stacking Adapter And Base Rack With Stacking Elements

    2-4) at the top of adapter plate. The devices are secured to one another by their own weight, only for the adapter plate (7/Figure 2-5) to the upper unit (10/Figure 2-5). HERA 160i LK / 250i LK CELL VIOS Thermo Scientific...

  • Page 30: Figure 2-6. Removing The Screws For The Wall Brackets

    CAUTION Transporting stacked devices! Stacking elements are no connection elements. Stacked devices provided with a mobile base rack must only be moved inside rooms on even floors without any slopes. HERA 160i LK / 250i LK CELL VIOS Thermo Scientific...

  • Page 31: Stacking Variants

    Steri-Cycle i250 LK HERAcell Vios  50148171 50154522 50148172 160i LK Steri-Cycle i160 LK HERAcell Vios  50154522 50148175 250i LK Steri-Cycle i250 LK Details see assembly instruction of stacking adapter kit. HERA 160i LK / 250i LK CELL VIOS Thermo Scientific...

  • Page 32: Installing The Support Stand With Castors

    Scope of Delivery 1 support stand, 6 screws M4x16 • Remove the 6 screws at the lower rear side of the unit. They will be replaced with the six new supplied screws. HERA 160i LK / 250i LK CELL VIOS Thermo Scientific...
  • Page 33 To avoid crushing your fingers or hands (particularly in a closing door) or damaging the incubator, do not use any other lift points than those indicated in the illustration above. HERA 160i LK / 250i LK CELL VIOS Thermo Scientific...
  • Page 34 Material Support stand Description Number Options 50145394 Support stand with castors for HERAcell VIOS 160i LK and Steri-Cycle i160 LK 50154407 Support stand with castors for HERAcell VIOS 160i LK and Steri-Cycle i160 LK 50154551 Support stand with castors for HERAcell VIOS 250i LK and...

  • Page 35: Retrofitting/Modifications

    (replaces the standard TCD sensor), • 4-20 mA data interface. • gas cylinder monitor Note Modifications:  Retrofittings and modifications must only be performed by the Technical Service of Thermo Electron LED GmbH. HERA 160i LK / 250i LK CELL VIOS Thermo Scientific 2-11...
  • Page 36 Installation of the Device Retrofitting/Modifications HERA 160i LK / 250i LK CELL VIOS 2-12 Thermo Scientific...
  • Page 37 Description of the Device Table of Contents • “HERACELL VIOS 160i LK / VIOS 250i LK Front View“ page 3-2 • “HERACELL VIOS 160i LK / VIOS 250i LK Rear View“ page 3-4 • “Safety Devices HERACELL VIOS 160i LK / VIOS 250i LK“...

  • Page 38: Chapter 3 Description Of The Device

    Description of the Device HERACELL VIOS 160i LK / VIOS 250i LK Front View HERA 160i LK / 250i LK Front View CELL VIOS VIOS Figure 3-1. HERA 160i LK 250i LK Front View CELL VIOS / VIOS HERA 160i LK / 250i LK...
  • Page 39 Description of the Device HERACELL VIOS 160i LK / VIOS 250i LK Front View 1. Pressure compensation opening /Access port with plug 2. Door switch for glass door 3. Temperature Sensor 4. O sensor (optional) 5. Door handle for the glass door or the gas-tight screen 6.

  • Page 40: Heracell Vios 160I Lk / Vios 250I Lk Rear View

    Description of the Device HERACELL VIOS 160i LK / VIOS 250i LK Rear View HERA 160i LK 250i LK Rear View CELL VIOS / VIOS Figure 3-2. HERA 160i LK 250i LK Rear View CELL VIOS / VIOS 1. Control box with supply interface for combined gas connection (optional) CO...

  • Page 41: Heracell Vios 250I Lk

    Description of the Device HERACELL VIOS 160i LK / VIOS 250i LK Rear View 11. Distributor connection for looping through the CO gas supply for up to three additional devices (independent of device type) Hidden: Distributor connection for looping through the O...

  • Page 42: Safety Devices Heracell Vios 160I Lk / Vios 250I Lk

    Description of the Device Safety Devices HERACELL VIOS 160i LK / VIOS 250i LK 5. Gas pressure hose for looping-through the gas supply 6. Diagram: CO and O gas connection with optional CO and O gas monitoring 7. HERA 160i LK / HERA 250i LK with looped-through gas supply;...

  • Page 43: Water Quality Recommendations

    The pre-filter must remain installed in the device during the Steri-run decontamination routine, but needs to be removed for refilling the water reservoir. HERA 160i LK / 250i LK CELL VIOS Thermo Scientific...

  • Page 44: Hepa-Filter And Air Duct

    3-5) to the workspace ceiling, thus ensuring an optimum temperature distribution. At the same time, it directs the incoming stream of process gases into workspace and ensures that the gases are optimally intermixed. HERA 160i LK / 250i LK CELL VIOS Thermo Scientific...

  • Page 45: Co2 Supply

    21 % to 90 %. Operation with higher oxygen concentrations requires that the fire protection caution notes in Preface in the section Safety Notes on Gases on page be heeded. HERA 160i LK / 250i LK CELL VIOS Thermo Scientific...

  • Page 46: N2 Supply

    The outer door can only be closed after the glass door has been latched properly. Note Versions with gas-tight screen:  For devices with the optional gas-tight screen, the door switch function described above is activated when the outer door is opened. HERA 160i LK / 250i LK CELL VIOS 3-10 Thermo Scientific...

  • Page 47: Sensor System

    When the water reservoir level falls to 0,5 l, the display field rH shows Error - rH - No water (see also “Error Messages“ page 6-45). HERA 160i LK / 250i LK CELL VIOS Thermo Scientific 3-11...

  • Page 48: Figure 3-8. Sensors For Water Level

    • the alarm relay responds. If the error message is accepted, the display shows the Overtemperature icon to indicate the response of the thermal protection, and the temperature display is highlighted in red. HERA 160i LK / 250i LK CELL VIOS 3-12 Thermo Scientific...

  • Page 49: Supply Interface

    Description of the Device Supply Interface Supply Interface Standard Interfaces Figure 3-9. Interfaces (Right Side of Control Box) HERA 160i LK / 250i LK CELL VIOS Thermo Scientific 3-13...

  • Page 50: Optional Interfaces

    2. 4...20 mA interface (option) 3. O connector (option, only available with CO - and O - gas supply sources and simultaneous use of gas monitoring option) HERA 160i LK / 250i LK CELL VIOS 3-14 Thermo Scientific...

  • Page 51: Gas Connection

    D/A converter. The D/A converter provides a resolution of 16 bit per channel, equivalent to 65536 incremental values. The interface port accepts various customer-supplied external measurement devices with 4-20 mA inputs - for example, the Thermo Scientific Smart-Vue Wireless Monitoring System (not supplied). The table below lists the output signals: Channel...

  • Page 52: Alarm Contact

    Mains power is connected to the unit using a cable with an IEC connector plugged into the IEC socket in the control box (7/Figure 3-9). The mains socket must be easily identifiable by the user and freely accessible at all times. HERA 160i LK / 250i LK CELL VIOS 3-16 Thermo Scientific...

  • Page 53: Workspace Components

    Glass Door and Optional Gas-Tight Screen The standard version of the HERA 160i LK and HERA 250i LK units is equipped with a CELL VIOS CELL VIOS single-locked door made of tempered safety glass. HERA 160i LK / 250i LK CELL VIOS Thermo Scientific 3-17...

  • Page 54: Figure 3-11. Heracell Vios 160I Lk With Glass Door

    160i LK: threefold gas-tight screen (see CELL VIOS Figure 3-12), • HERA 250i LK: sixfold gas-tight screen. CELL VIOS • Optional 6-fold gas tight screen with Cell locker (see Appendix). HERA 160i LK / 250i LK CELL VIOS 3-18 Thermo Scientific...

  • Page 55: Water Reservoir

    The pre-filter must remain installed in the device during the Steri-run decontamination routine, but needs to be removed for refilling the water reservoir. Additional instruction when filling with 6-door gas tight screen, see Appendix. HERA 160i LK / 250i LK CELL VIOS Thermo Scientific 3-19...

  • Page 56: Figure 3-13. Water Reservoir

    Plugging the drain tube supplied into the front-side quick-drain valve starts draining immediately. Figure 3-14. Fill and Drain Valve of Water Reservoir HERA 160i LK / 250i LK CELL VIOS 3-20 Thermo Scientific...

  • Page 57: Water Filling

    The outer door of the unit and the perimeter of the door opening are heated as well. The heat radiated onto the interior glass door/gas-tight screen prevents condensate formation. The workspace of the device always remains visible, despite high humidity. HERA 160i LK / 250i LK CELL VIOS Thermo Scientific 3-21...

  • Page 58: Rear Panel Openings

    To avoid damage to the silicone, sure to remove the silicone plug from the inner chamber and plug it into the outer side of the access port before starting the high-temperature decontamination routine. HERA 160i LK / 250i LK CELL VIOS 3-22 Thermo Scientific...

  • Page 59: Shelf System

    160i LK with a cutout for refilling CELL VIOS (4/Figure 3-18). For details on using the shelf system, “Start-up“ page 4-1. Additional instruction when using shelf with 6-fold gas tight screen, see Appendix. HERA 160i LK / 250i LK CELL VIOS Thermo Scientific 3-23...

  • Page 60: Electromechanical Door Lock Assembly

    Detail C of Figure 3-19 presents a breakout view through the side panel to illustrate the lever location. HERA 160i LK / 250i LK CELL VIOS 3-24 Thermo Scientific...

  • Page 61: Chapter 4 Start-Up

    “Mount the Split Inserts (Optional) into the HERACELL VIOS 250i LK“ page 4-11 • “Gas Connection“ page 4-11 • “Power Supply Connection“ page 4-16 • “Connecting the USB Interface:“ page 4-17 • “Connecting the Alarm Contact:“ page 4-18 HERA 160i LK / 250i LK CELL VIOS Thermo Scientific...

  • Page 62: Adapting The Unit To The Ambient Atmosphere

    CAUTION Observe the instructions or technical data of the manuals delivered with the spare parts or accessories. The instructions or technical data can differ from them stated in this manual. HERA 160i LK / 250i LK CELL VIOS Thermo Scientific...

  • Page 63: Installing The "Max" Fill Level Indicator And The Pre-Filter

    3. Suspend the “MAX” fill level indicator (1/Figure 4-1) in the slot provided in the water reservoir cover panel (5/Figure 4-1). Figure 4-1. “MAX” Fill Level Indicator HERA 160i LK / 250i LK CELL VIOS Thermo Scientific...

  • Page 64: Figure 4-2. "Max" Fill Level Indicator And Pre-Filter

    Installing the “MAX” Fill Level Indicator and the Pre-Filter 4. Insert the pre-filter (4/Figure 4-2) into the water reservoir cover panel. 1, 2 Figure 4-2. “MAX” Fill Level Indicator and Pre-Filter HERA 160i LK / 250i LK CELL VIOS Thermo Scientific...

  • Page 65: Install The Air Duct

    4-3) on the two rear wall studs and tilt the air duct backwards. 3. Lock the lateral keyholes of the top part (step G/Figure 4-3) into the retaining screws in the workspace ceiling. Figure 4-3. Assembling the Air Duct HERA 160i LK / 250i LK CELL VIOS Thermo Scientific...
  • Page 66 160i LK models, do not remove the sealings of the air ducts before installation CELL VIOS in the rear wall. The rear wall air ducts are important for a proper air flow on VIOS160 models with a stainless steel inner chamber. HERA 160i LK / 250i LK CELL VIOS Thermo Scientific...

  • Page 67: Installing The Hepa-Filter And The Water Reservoir Cover Panel

    4-5) on the right side of the airbox into the HEPA-filter catches (6/Figure 4-5). 4. Place the seal (8/Figure 4-5) into the groove (7/Figure 4-5) provided at the airbox tubing and press it down evenly. HERA 160i LK / 250i LK CELL VIOS Thermo Scientific...

  • Page 68: Figure 4-6. Place The Airbox Onto The Water Reservoir Cover Panel

    9. Let the front edge of the water reservoir cover slide into the water reservoir (2/Figure 4-7). The airbox tubing slides into the fan outlet. HERA 160i LK / 250i LK CELL VIOS Thermo Scientific...

  • Page 69: Installing The Shelf System

    2. Clamp the lockspring behind the upper embossing. 3. To remove the support rails, pull the lockspring tab down out of the embossing and remove the rail. HERA 160i LK / 250i LK CELL VIOS Thermo Scientific...

  • Page 70: Shelf Support Installation

    For 6-fold gas tight screen the shelf supports are installed in the rectangular holes only, installing the shelves, see Appendix. Levelling the Device 1. Position a bubble level onto the center shelf or onto the roller holder. HERA 160i LK / 250i LK CELL VIOS 4-10 Thermo Scientific...

  • Page 71: Mount The Split Inserts (Optional) Into The Heracell Vios 250I Lk

    4-10) in the shelf supports (3/Figure 4-10) (step B). Gas Connection Note Gas quality: The gases must have one of the following qualities: • Purity 99.5 % min • medical gas quality. HERA 160i LK / 250i LK CELL VIOS Thermo Scientific 4-11...

  • Page 72: Installing Gas Pressure Hoses

    To ensure permanent pressure compensation, the pressure compensation opening must not be connected to an exhaust air system. The pipe of the pressure compensation opening must not be extended or redirected. HERA 160i LK / 250i LK CELL VIOS 4-12 Thermo Scientific...

  • Page 73: Gas Connection Without Gas Monitoring System

    4-12). Combined CO and O Connection (Optional): On units with a combined CO connection, the gas supply tubes must be routed according to the following connection diagram (3/Figure 4-12): HERA 160i LK / 250i LK CELL VIOS Thermo Scientific 4-13...

  • Page 74: Co2 Connection With Gas Monitoring System (Optional)

    For looping the gas supply through, the devices must be interconnected with a gas pressure hose. • Item 2/Figure 4-13: Connect the gas container B to the upper connection of the gas monitoring system. HERA 160i LK / 250i LK CELL VIOS 4-14 Thermo Scientific...

  • Page 75: Combined Co2 And O2/N2 Connection With Gas Monitoring System (Optional)

    • Item 1/Figure 4-14: The outlet of the gas monitoring system is connected at the factory to the gas inlet filter (item 8/Figure 4-14) by a short gas pressure hose. HERA 160i LK / 250i LK CELL VIOS Thermo Scientific 4-15...

  • Page 76: Power Supply Connection

    By looping the gas supply through, up to 4 devices (independent of device type) can be supplied by a CO gas supply or a O gas supply. Power Supply Connection Figure 4-15. Power Supply Connection HERA 160i LK / 250i LK CELL VIOS 4-16 Thermo Scientific...

  • Page 77: Connecting The Usb Interface

    The bit rate of the interface can be changed within the pre-set Baud rates limits (9.600, 19.200, 38.400, 57.600 Baud). Baud rate adjustment is described in section “USB Interface Baud Rate Setting“ page 6-23. HERA 160i LK / 250i LK CELL VIOS Thermo Scientific 4-17...

  • Page 78: Connecting The Alarm Contact

    Connecting the Alarm Contact: Note Skilled work: Thermo Scientific warrants the operational safety and the operativeness of the device only if installation and repairs are performed properly. The connection of the device to an external alarm system must only be carried out by adequately trained and authorized expert electrical/telecommunication personnel! Function:...

  • Page 79: Figure 4-16. Connection Example Alarm Contact

    1. Wire the individual conductors as shown in the wiring diagram. 2. Connect the connector of the alarm system connecting cable to the interface at the control box at the rear panel of the device. HERA 160i LK / 250i LK CELL VIOS Thermo Scientific 4-19...

  • Page 80: Connecting The 4-20 Ma Interface

    Figure 4-17. Pin-out of the 4-20 mA Interface Note Skilled work: Thermo Scientific warrants the operational safety and the operativeness of the device only if installation and repairs are performed properly. The connection of the device to an external alarm system must only be carried out by adequately...

  • Page 81: Chapter 5 Operation

    121°C/250 °F. • Run the steri-run decontamination routine (see “Steri-Run Decontamination Routine“ page 8-7) or decontaminate the workspace according to the hygiene regulations set forth by the operator. HERA 160i LK / 250i LK CELL VIOS Thermo Scientific...

  • Page 82: Starting Operation

    CELL VIOS Single-piece insert (HERA the container used for filling. 4. Fill reservoir with sufficiently purified water (beneath cover item 1 in Figure 5-1) of the workspace. Figure 5-1. Water Reservoir HERA 160i LK / 250i LK CELL VIOS Thermo Scientific...

  • Page 83: Water Filling

    • Fill the funnel to the top to facilitate gravity to fill with sterile distilled water. This may take up to several times. • Keep adding until the maximum level indicator is reached. HERA 160i LK / 250i LK CELL VIOS Thermo Scientific...

  • Page 84: Figure 5-4. "Max" Fill Level Indicator

    CO 17. If the auto-start routine has been completed, the progress indicator is hidden, and the main menu is displayed. The device is operational. HERA 160i LK / 250i LK CELL VIOS Thermo Scientific...
  • Page 85 70% max. Bulky objects in the workspace that dissipate heat may impair heat distribution. Bulky objects in the use able space that dissipate heat may impair heat distribution. HERA 160i LK / 250i LK CELL VIOS Thermo Scientific...
  • Page 86 Operation Water Filling HERA 160i LK / 250i LK CELL VIOS Thermo Scientific...

  • Page 87: Chapter 6 Handling And Control

    6-12 • “Launching Steri-Run“ page 6-16 • “User Configuration“ page 6-17 • “Trend Display Scaling“ page 6-43 • “Error Messages“ page 6-45 • “Measures After Power Outage“ page 6-47 HERA 160i LK / 250i LK Thermo Scientific CELL VIOS...

  • Page 88: Power Switch

    • The control loop sensors pass the heat-up phase (“Heat-up Phase of the Control Loop Sensors“ page 6-7). • Powering down: Press the power switch; the switch illumination goes off. HERA 160i LK / 250i LK CELL VIOS Thermo Scientific...

  • Page 89: Operating Panel And Operating Structure

    6-40), • steri-run key, • Menu key, • auto-start key. Note Extended pressure-sensitive area: To accept a failure message, the entire touchscreen can be used as a pressure-sensitive area. HERA 160i LK / 250i LK Thermo Scientific CELL VIOS...

  • Page 90: Versions Without O2/N2 Control

    6-40) 4. Key for starting the steri-run decontamination routine 5. Key for opening the menu navigation 6. Key for starting the auto-start routine 7. Display field water level RH HERA 160i LK / 250i LK CELL VIOS Thermo Scientific...

  • Page 91: Structure Of The Operating Levels

    • A: Direct access to the control loop settings: Temperature, CO -setpoint, • B: Starting the Steri-Run or auto-start routine of the unit, • C: Navigation through the submenus for device configuration. HERA 160i LK / 250i LK Thermo Scientific CELL VIOS...

  • Page 92: Figure 6-5. Menu Structure Overview

    Handling and Control Operating Panel and Operating Structure Figure 6-5. Menu Structure Overview HERA 160i LK / 250i LK CELL VIOS Thermo Scientific...

  • Page 93: Factory Presettings Of The Ican™ Touchscreen Controls

    The start process is indicated by an audible signal. During the heat-up phase, the displays show dots (...) instead of values: • Temperature display, • CO display and • O display field HERA 160i LK / 250i LK Thermo Scientific CELL VIOS...

  • Page 94: Behavior Of Keys During Adjustments

    Figure 6-7. Temperature Display Field and Temperature Selection Menu 2. To exit the Temperature menu: • Press the End key [3]. 3. To set the temperature set value: • Press the Set value key. HERA 160i LK / 250i LK CELL VIOS Thermo Scientific...

  • Page 95: Setting The Co2 Set Value

    1. Press the CO display key. • The CO menu is displayed. Figure 6-9. CO Display and CO Menu 2. To exit the CO menu: • Press the End key [3]. HERA 160i LK / 250i LK Thermo Scientific CELL VIOS...

  • Page 96: Setting The O2 Set Value

    Setting the O Set Value This setting is possible only on versions with the optional O control. 1. Press the O display key. • The O menu is displayed. HERA 160i LK / 250i LK 6-10 CELL VIOS Thermo Scientific...

  • Page 97: Figure 6-11. O2 Display And O2 Menu

    • Press the Enter key. • The system returns to the main menu. The O display shows the actual value currently measured in the workspace. Figure 6-12. Setting the O Set Value HERA 160i LK / 250i LK Thermo Scientific CELL VIOS 6-11...

  • Page 98: Auto-Start Function

    When temperature and relative humidity have reached constant values, the measuring system is automatically adjusted to these values, and the workspace is supplied with the preset quantity of CO HERA 160i LK / 250i LK 6-12 CELL VIOS Thermo Scientific...
  • Page 99 • No communication with sensor. Gas supply CO control loop: • No communication with sensor. In this case, the auto-start key is dimmed and its function is not available. HERA 160i LK / 250i LK Thermo Scientific CELL VIOS 6-13...

  • Page 100: Activating The Auto-Start Routine

    3. Refill water, if necessary. 4. Activate the auto-start routine: • Press the Start key. 5. To air the workspace, open both device doors. When the audible alarm sounds after 30 seconds, HERA 160i LK / 250i LK 6-14 CELL VIOS Thermo Scientific...

  • Page 101: Interrupting The Auto-Start Routine

    • The system returns to the status display, and the auto-start routine is resumed. 2. To cancel the auto-start routine: • Press the End key [3]. • The warning triangle is displayed as a failure message together with the audible signal. HERA 160i LK / 250i LK Thermo Scientific CELL VIOS 6-15...

  • Page 102: Launching Steri-Run

    An in-depth description of the function is given in the chapter Cleaning and disinfection (Chapter 8‚ page HERA 160i LK / 250i LK 6-16 CELL VIOS Thermo Scientific...

  • Page 103: User Configuration

    • Changing the keypad lock code, • Date/time setting, • Adjusting display brightness, • Key tone setting, • Interface configuration, • User display screen language setting, • Reminder interval setting. HERA 160i LK / 250i LK Thermo Scientific CELL VIOS 6-17...

  • Page 104: Figure 6-18. Settings/Setup Menu

    Figure 6-19. Changing the Keypad Lock Code 3. To enter the default 0000: • Press the corresponding numeric keys. • The number combination is displayed as encrypted text in the input box. HERA 160i LK / 250i LK 6-18 CELL VIOS Thermo Scientific...

  • Page 105: Figure 6-20. Changing The Keypad Lock Code

    The input dialog allows date and time to be set to the required time zone. 1. Press the Menu key. 2. Select the menu command Date / Time. • The selection dialog shown in Figure 6-21 is displayed. HERA 160i LK / 250i LK Thermo Scientific CELL VIOS 6-19...

  • Page 106: Figure 6-21. Date/Time Menu

    • Press the Enter key. 7. The system returns to the Date/Time menu. 8. For changing the time, choose the Time option. • The input dialog shown in Figure 6-23 is displayed. HERA 160i LK / 250i LK 6-20 CELL VIOS Thermo Scientific...

  • Page 107: Figure 6-23. Setting The Time

    1. Press the Menu key. 2. Select the menu command Settings / Setup. 3. Select the option Display contrast. • The input dialog shown in Figure 6-24 is displayed. HERA 160i LK / 250i LK Thermo Scientific CELL VIOS 6-21...

  • Page 108: Figure 6-24. Adjusting Display Brightness

    1. Press the Menu key. 2. Select the menu command Settings / Setup. 3. Select the option Key tones. • The input dialog shown in Figure 6-25 is displayed. HERA 160i LK / 250i LK 6-22 CELL VIOS Thermo Scientific...

  • Page 109: Figure 6-25. Adjusting Key Tone Volume

    The baud rate can be changed within the defined baud rates (9,600, 19,200, 38,400, 57,600 baud). Setting the Baud Rate 1. Press the Menu key. 2. Select the menu command Settings / Setup. • The selection dialog shown in Figure 6-26 is displayed. HERA 160i LK / 250i LK Thermo Scientific CELL VIOS 6-23...

  • Page 110: Figure 6-26. Interface Usb Menu

    7. To activate the new settings: • Browse back to the main menu. • Wait for approx. 10 seconds and perform a reset by switching the device off and on using the power switch. HERA 160i LK / 250i LK 6-24 CELL VIOS Thermo Scientific...

  • Page 111: Figure 6-28. Language Setting

    5. The new language appears in the display. The New message indicates that the selection has been changed, but not saved yet. 6. To accept and save the selection: • Press the Enter key. • The system returns to the Settings/Setup menu. HERA 160i LK / 250i LK Thermo Scientific CELL VIOS 6-25...

  • Page 112: Figure 6-29. Choose The Desired Reminder Interval Function

    Figure 6-29. Choose the Desired Reminder Interval Function. 3. Select the appropriate menu option, for example Steri-run. • The input dialog shown in Figure 6-29 is displayed. HERA 160i LK / 250i LK 6-26 CELL VIOS Thermo Scientific...

  • Page 113: Data Logging

    (Figure 6-31) allows for accessing dialog screens for logging and displaying events that occur during ongoing operation of the unit: • Event display, • Recording interval, • Error table. HERA 160i LK / 250i LK Thermo Scientific CELL VIOS 6-27...

  • Page 114: Figure 6-31. Event Logging Menu

    • The selection dialog shown in Figure 6-31 is displayed. 3. Select the menu command Event display. • The list shown in Figure 6-32 appears on the display. Figure 6-32. Event Display HERA 160i LK / 250i LK 6-28 CELL VIOS Thermo Scientific...

  • Page 115: Figure 6-33. Logging Cycle Setting

    • The selection dialog shown in Figure 6-31 is displayed. 3. Select the menu command Recording interval. • The input dialog shown in Figure 6-33 is displayed. Figure 6-33. Logging Cycle Setting HERA 160i LK / 250i LK Thermo Scientific CELL VIOS 6-29...
  • Page 116 2. Select the menu command Event logging. • The selection dialog shown in Figure 6-31 is displayed. 3. Select the menu command Event display. • The list shown in Figure 6-34 appears on the display. HERA 160i LK / 250i LK 6-30 CELL VIOS Thermo Scientific...

  • Page 117: Options

    6-35) allows for accessing all selection dialogs for the functional options of the unit: • Alarm, • Low humidity, • Gas-tight screen (optional), • Humidity sensor, • Audible alarm, • O gas supply (optional), • HEPA-filter. HERA 160i LK / 250i LK Thermo Scientific CELL VIOS 6-31...

  • Page 118: Figure 6-35. Options Menu

    • The selection dialog shown in Figure 6-36 is displayed. Figure 6-36. Alarm Menu 4. Select the menu command Alarm relay. • The input dialog shown in Figure 6-37 is displayed. HERA 160i LK / 250i LK 6-32 CELL VIOS Thermo Scientific...

  • Page 119: Figure 6-37. Alarm Relay Setting

    2. Select the menu command Options. • The selection dialog shown in Figure 6-35 is displayed. 3. Select the menu command Low humidity. • The input dialog shown in Figure 6-38 is displayed. HERA 160i LK / 250i LK Thermo Scientific CELL VIOS 6-33...

  • Page 120: Figure 6-38. Low Humidity Setting

    Gas Tight Screen Setting 1. Press the Menu key. 2. Select the menu command Options. • The selection dialog shown in Figure 6-35 is displayed. HERA 160i LK / 250i LK 6-34 CELL VIOS Thermo Scientific...

  • Page 121: Figure 6-39. Gas Tight Screen Setting

    2. Select the menu command Options. • The selection dialog shown in Figure 6-35 is displayed. 3. Select the menu command Water level sensor. • The input dialog shown in Figure 6-40 is displayed. HERA 160i LK / 250i LK Thermo Scientific CELL VIOS 6-35...

  • Page 122: Figure 6-40. Setting The Water Level Sensor

    2. Select the menu command Options. • The selection dialog shown in Figure 6-35 is displayed. 3. Select the menu command Buzzer. • The input dialog shown in Figure 6-41 is displayed. HERA 160i LK / 250i LK 6-36 CELL VIOS Thermo Scientific...

  • Page 123: Figure 6-41. Alarm Relay Setting

    2. Select the menu command Options. • The selection dialog shown in Figure 6-35 is displayed. 3. Select the menu command O • The input dialog shown in Figure 6-42 is displayed. HERA 160i LK / 250i LK Thermo Scientific CELL VIOS 6-37...
  • Page 124 The gas monitoring system remains active even when the O control is switched off or the -sensor is disabled. HERA 160i LK / 250i LK 6-38 CELL VIOS Thermo Scientific...

  • Page 125: Figure 6-43. Hepa Configuration

    Figure 6-44. Activating / De-Activating the HEPA-Filter 1. To toggle between two states: • Press the + key. • Press the - key. 2. To accept and save the change: HERA 160i LK / 250i LK Thermo Scientific CELL VIOS 6-39...

  • Page 126: Icon Description

    The monitoring function is available only for device versions with the optional gas monitoring system. Overtemperature: This error indicator shows that the device control has activated the overtemperature protection and has switched to backup control. HERA 160i LK / 250i LK 6-40 CELL VIOS Thermo Scientific...

  • Page 127: Figure 6-46. Gas Monitoring Icons

    • Manual changeover to a full gas cylinder is possible. • Press the cylinder icon with the blue frame.  • changeover can occur automatically, if: • the cylinder pressure drops below 0.6 bar (8.7 psi). HERA 160i LK / 250i LK Thermo Scientific CELL VIOS 6-41...

  • Page 128: Enabling/Disabling The Keypad Lock

    Resetting the code: If the keypad lock code is no longer available, the code must be reset to the standard code by the Technical Support of Thermo Fisher Scientific. HERA 160i LK / 250i LK 6-42 CELL VIOS Thermo Scientific...

  • Page 129: Software Versions

    • temperature, • 0...20% CO • 0...100% O can be scaled to two different versions. 1. Full screen display • Press the CO display field in the main menu. HERA 160i LK / 250i LK Thermo Scientific CELL VIOS 6-43...
  • Page 130 • During zoom mode the trend display can be scrolled forward and back. 4. To return to the total trend: • Open rectangle over a small diagram section and press any position outside the marked area. HERA 160i LK / 250i LK 6-44 CELL VIOS Thermo Scientific...

  • Page 131: Error Messages

    • The Error dialog box appears and the detected error is displayed, • the audible alarm is switched off. 2. To exit the error display: • Press the End key [3]. • The error message disappears. HERA 160i LK / 250i LK Thermo Scientific CELL VIOS 6-45...

  • Page 132: Resetting Overtemperature Protection

    1. Displaying the cause of an error: • Press anywhere on the touchscreen. • The Error dialog box appears and the detected error is displayed. • The audible alarm is switched off. HERA 160i LK / 250i LK 6-46 CELL VIOS Thermo Scientific...

  • Page 133: Measures After Power Outage

    Alternatively, or when the above mentioned measures were not effective, start a disinfection run at 180 °C. Refer to Chapter “Steri-Run Decontamination Routine“ page 8-7. The disinfection routine can be interrupted after approx. 1 hour. The sensors should then have dried. HERA 160i LK / 250i LK Thermo Scientific CELL VIOS 6-47...
  • Page 134 Error: Fan Actual value of the fan Reset unit. If the error is out of tolerance. occurs repeatedly, call service. IR sensor changed New serial number Acknowledge the detected alarm HERA 160i LK / 250i LK 6-48 CELL VIOS Thermo Scientific...
  • Page 135 Reset unit. If the error plausible temperature signal occurs repeatedly, call service. Calibration values too Max. adjust value for Acknowledge alarm, high/low temperature exceeded / enter different target not attained value. HERA 160i LK / 250i LK Thermo Scientific CELL VIOS 6-49...
  • Page 136 “Measures After Power Outage“ page 6-47. Cylinder A empty Gas cylinder A is empty Replace gas cylinder A Cylinder B empty Gas cylinder B is empty Replace gas cylinder B HERA 160i LK / 250i LK 6-50 CELL VIOS Thermo Scientific...
  • Page 137  - 45 min. after a door was opened,  - 159 min. after a setpoint change. This setting can be changed by field service. HERA 160i LK / 250i LK Thermo Scientific CELL VIOS 6-51...
  • Page 138 Handling and Control Measures After Power Outage HERA 160i LK / 250i LK 6-52 CELL VIOS Thermo Scientific...

  • Page 139: Chapter 7 Shut-Down

    Figure 7-1. Fill and Drain Valve of Water Reservoir 5. Allow the water reservoir to drain completely into the collecting vessel. 6. Remove drain hose valve again. 7. Start the Steri-Run decontamination routine („Launching Steri-Run“ page 6-16). HERA 160i LK / 250i LK CELL VIOS Thermo Scientific...
  • Page 140 11. Disconnect the gas pressure hoses from the sleeve at the rear of the device. 12. Until the device is shut down, the workspace must be continuously ventilated. Leave the glass door and the outer door open and secure them in this state. HERA 160i LK / 250i LK CELL VIOS Thermo Scientific...

  • Page 141: Chapter 8 Cleaning And Disinfection

    3. Then, wipe the surfaces dry using a clean cloth. Cleaning Display: CAUTION Moisture-sensitive display! Do not spray or wipe the display with cleaner. • Clean display using a dry cloth of 100% micro fiber! HERA 160i LK / 250i LK CELL VIOS Thermo Scientific...

  • Page 142: Decontamination Procedures

    7. Pull airbox (1/Figure 8-2) out of its seat on the water reservoir cover panel and remove it. 8. Take HEPA-filter (2/Figure 8-2) out of airbox (1/Figure 8-2) and reinstall the airbox. HERA 160i LK / 250i LK CELL VIOS Thermo Scientific...

  • Page 143: Wipe/Spray Disinfection

    Prior to cleaning and disinfection work, disconnect the device from the power supply! • Turn the device off using the power switch. • Unplug the power connector and protect it against accidental reconnection. • Make sure the device is de-energized. HERA 160i LK / 250i LK CELL VIOS Thermo Scientific...
  • Page 144 “Installing the Shelf System“ page 4-9. 2. Remove the airbox and the HEPA-filter. The installation and removal of the airbox and the HEPA-filter are described in section “Replacing the HEPA-Filter“ page 9-7. HERA 160i LK / 250i LK CELL VIOS Thermo Scientific...

  • Page 145: Figure 8-2. Hepa-Filter And Airbox

    4. Separate the top part from the rear part of the air duct (2/Figure 8-3) and remove it from the workspace. HERA 160i LK / 250i LK CELL VIOS Thermo Scientific...

  • Page 146: Figure 8-3. Air Duct

    1. Spray the surfaces of the workspace, the shelving system and parts removed in previous steps with disinfectant one more time and wipe dry. 2. Allow the disinfectant to react as specified by the manufacturer. 3. Reinstall the shelf system and the removed components. HERA 160i LK / 250i LK CELL VIOS Thermo Scientific...

  • Page 147: Steri-Run Decontamination Routine

    90 minutes. The effectiveness of the steri-run decontamination routine has been certified by independent institutes. The germ reduction efficiency achieved is equal to 10 (6-log reduction) to the ISO 11138 standard. On request, Thermo Scientific will supply information on the pertinent tests.

  • Page 148: Progress Of A Steri-Run Decontamination Routine

    Heating phase: approx. 2 h. The workspace is heated up to 180 °C.  Incubators equipped with the electromagnetic door lock assembly lock the door when the workspace temperature surpasses 65°C. HERA 160i LK / 250i LK CELL VIOS Thermo Scientific...

  • Page 149: Activating Steri-Run

    Steri-Run decontamination routine. • The steri-run menu is displayed. Figure 8-5. Steri-Run Menu - Progress 2. steri-run menu - Exit and stop steri-run: HERA 160i LK / 250i LK CELL VIOS Thermo Scientific...

  • Page 150: Canceling Steri-Run

    • If the Stop key in the status display is depressed, the steri-run stop dialog box is displayed for a safety scan. The routine can now be permanently canceled or resumed. 2. To stop steri-run: HERA 160i LK / 250i LK CELL VIOS 8-10 Thermo Scientific...

  • Page 151: Steri-Run Interruption Due To Error

    • Press the End key. • The error message is displayed. • If the error message is confirmed, the system returns to the main menu. Figure 8-7. Interrupting / Canceling Steri-Run HERA 160i LK / 250i LK CELL VIOS Thermo Scientific 8-11...

  • Page 152: Completing Steri-Run

    If the door will be opened and closed again whilst steri-run decontamination is running, the routine returns to a phase which will ensure a continuation without faults. Note  Risk of burns! Avoid opening the door unless in case of an emergency. HERA 160i LK / 250i LK CELL VIOS 8-12 Thermo Scientific...

  • Page 153: Opening The Door After Canceling Steri-Run

    During the routine run or immediately after completion of the run, always wear safety gloves when touching these surfaces! HERA 160i LK / 250i LK CELL VIOS Thermo Scientific 8-13...
  • Page 154 Cleaning and Disinfection Steri-Run Decontamination Routine HERA 160i LK / 250i LK CELL VIOS 8-14 Thermo Scientific...
  • Page 155 Note Functional check: If safety devices were removed or disabled for inspections, the device must not be operated before the safety devices have been reinstalled and checked for their correct function. HERA 160i LK / 250i LK CELL VIOS Thermo Scientific...

  • Page 156: Chapter 9 Maintenance

    • Perform Technical Service service inspection. Note Service contract: Thermo Scientific offer a device-specific service contact that comprises all test and service works required. Preparing Temperature Calibration To determine the exact measured value of the incubator’s integral temperature sensor, a temperature comparison measurement must be performed every three months.

  • Page 157: Temperature Calibration Procedure

    6. Temperature calibration procedure Temperature Calibration Procedure Measurement example: • Temperature set value: 37 °C Reference temperature: 36.4 °C 1. Press the Temperature Display key. • The Temperature menu (Figure 9-2) is displayed. HERA 160i LK / 250i LK CELL VIOS Thermo Scientific...

  • Page 158: Figure 9-2. Temperature Display Field And Temperature Selection Menu

    • Press the + key. To reduce the set value: • Press the - key. 5. Accept and save the target value: • Press the Enter key. • Press the Save key. HERA 160i LK / 250i LK CELL VIOS Thermo Scientific...

  • Page 159: Preparing The Co2 Calibration

     concentration has been set to 4.0% CO Calibration should be performed with the CO set value designated for the work process (prospective work process value). HERA 160i LK / 250i LK CELL VIOS Thermo Scientific...

  • Page 160: Co2 Calibration Procedure

    • The CO menu is displayed. 2. To exit the CO menu: • Press the End key [3]. 3. To enter the Calibration submenu: • Press the Calibration key. HERA 160i LK / 250i LK CELL VIOS Thermo Scientific...

  • Page 161: Replacing The Hepa-Filter

    Steps for replacing the HEPA-filter: 1. Power down the unit, shut off the gas supply and allow the workspace to vent. 2. Take the center and bottom insert out of the workspace. HERA 160i LK / 250i LK CELL VIOS Thermo Scientific...

  • Page 162: Figure 9-6. Removing The Airbox

    9-7)on the left-hand side from the HEPA filter tabs (6/Figure 9-7). 7. Unlatch the tabs on the right-hand side (3/Figure 9-7) of the airbox (1/Figure 9-7) from the corresponding slots provided in the HEPA-Filter (4/Figure 9-7). HERA 160i LK / 250i LK CELL VIOS Thermo Scientific...

  • Page 163: Replacing The Gas Inlet Filter

    Procedure for gas supply gas inlet filter: 1. Make sure that the gas supply is shut off. 2. Loosen the hose clamp (4/Figure 9-8). HERA 160i LK / 250i LK CELL VIOS Thermo Scientific...

  • Page 164: Device Fusing Replacement

    The device’s fuses are not user-replaceable. If the unit fails due to an electrical fault, call Technical Field Service. Replacing the Door Seal Note  We recommend having the door seal replaced by a service technician or by expert staff. HERA 160i LK / 250i LK CELL VIOS 9-10 Thermo Scientific...

  • Page 165: Chapter 10 Waste Disposal

    Plastic components, general ABS and PPS GF40, note material identification Exterior housing Galvanized and painted steel sheet, Stainless steel 1.4016 Incubator rear panel Galvanized steel sheet HERA 160i LK / 250i LK CELL VIOS Thermo Scientific 10-1...
  • Page 166 Gas filter with polypropylene casing and GF/PTFE membrane, Pre-filter, stainless steel 1.4401 wire mesh Packaging Corrugated cardboard, polyethylene foil, styrofoam shaped elements and polypropylene Door seal magnet Permanent magnet Cell locker Polycarbonate Makrolon 2528 HERA 160i LK / 250i LK CELL VIOS 10-2 Thermo Scientific...

  • Page 167: Chapter 11 Specifications

    Specifications Content • “HERACELL VIOS 160i LK“ page 11-2 • “HERACELL VIOS 250i LK“ page 11-6 HERA 160i LK / 250i LK CELL VIOS Thermo Scientific 11-1...
  • Page 168 Specifications HERACELL VIOS 160i LK HERA 160i LK CELL VIOS Name Unit Value Mechanical Outer dimensions (W x H x D) 637 x 905 x 790 (25.08 x 35.63 x 31.10 inch) Inner dimensions (W x H x D) 470 x 607 x 576...
  • Page 169 Specifications HERACELL VIOS 160i LK Name Unit Value Constant humidity at 37 °C (high humidity mode) %rH approx. 93 Constant humidity at 37 °C (low humidity mode) approx. 90 Others Sound pressure level (DIN 45 635, part 1) dB(A) < 50 Relative ambient humidity max.
  • Page 170 Specifications HERACELL VIOS 160i LK HERA 160i LK CELL VIOS Name Unit Value gas supply system Gas purity min. 99.5 or med. quality Primary pressure min. 0.8 - max. 1 (11.6-14.5 psi) Measurement and control range Vol -% 0...20 Control deviation over time Vol -% ±...
  • Page 171 Specifications HERACELL VIOS 160i LK Name Unit Value 230 V:  Rated power Decontamination: 1.10 Incubation: 0.56 220 V:  Decontamination: 0.97 Incubation: 0.51 120 V:  Decontamination: 1.00 Incubation: 0.55 100 V:  Decontamination: 0.72 Incubation: 0.39 EMV class...

  • Page 172: Heracell Vios 250I Lk

    0.07 during steri-run decontamination kWh/h 0.75 Humidity Water qualities Electrical resistance: 50 kOhmcm to 1 MOhmcm Conductivity: 1 to 20 S/cm Capacities: max. 3 / min 0.5 Incubation operation HERA 160i LK / 250i LK CELL VIOS 11-6 Thermo Scientific...
  • Page 173 80 Altitude of site m above sea max. 2000 level determined on the basis of DIN 12880 for devices with standard feature set. See calibration instructions for detailed information. HERA 160i LK / 250i LK CELL VIOS Thermo Scientific 11-7...
  • Page 174 Decontamination: 5.5 Incubation: 3.3 220 V:  Decontamination: 5.3 Incubation: 3.2 120 V:  Decontamination: 10.4 Incubation: 6.3 100 V:  Decontamination: 8.9 Incubation: 5.3 Circuit breaker 16 A HERA 160i LK / 250i LK CELL VIOS 11-8 Thermo Scientific...
  • Page 175 Rated power Decontamination: 1.26 Incubation: 0.76 220 V:  Decontamination: 1.17 Incubation: 0.69 120 V:  Decontamination: 1.25 Incubation: 0.75 100 V:  Decontamination: 0.89 Incubation: 0.53 EMV class HERA 160i LK / 250i LK CELL VIOS Thermo Scientific 11-9...
  • Page 176 Specifications HERACELL VIOS 250i LK HERA 160i LK / 250i LK CELL VIOS 11-10 Thermo Scientific...

  • Page 177: Chapter 12 Data Communication

    “Examples of Data Logger Codes“ on page 12-15 • “Program HERACELL VIOS 160i LK / HERACELL VIOS 250i LK“ on page 12-21 USB Interface The units come equipped with a USB interface. The USB interface complies with Standard USB 1.1 / USB 2.0 / USB 3.0 (full speed).

  • Page 178: Installing The Usb Port Driver

    As soon as the Windows Hardware Detector has identified the USB port, the Find New Hardware Wizard dialog box opens. 1. Choose the INSTALL FROM A LIST OR SPECIFIC LOCATION (ADVANCED) option. HERA 160i LK / 250i LK CELL VIOS 12-2 Thermo Scientific...
  • Page 179 4. On the data CD, select the DRIVER sub-directory. 5. The installation routine installs the driver: EVAL22 Board USB. After the installation has been completed successfully, the routine is completed with FINISH. HERA 160i LK / 250i LK CELL VIOS Thermo Scientific 12-3...

  • Page 180: Structure Of The Command Sequences For Data Communication

    = checksum: CRC8-CCITT: x8 + x2 + x1 + 1 = 0x07  without cc and <CR> XXXX = bb-bytes user data Response with an error message: Response: !:aaaa:bb:XX:cc<CR> HERA 160i LK / 250i LK CELL VIOS 12-4 Thermo Scientific...

  • Page 181: Overview Of General Parameters (Addresses 0Xxx)

    Incubator parameters are divided into: • the basic parameters of the three control loops temperature, CO and O • the internal function parameters of operating functions and data logging. HERA 160i LK / 250i LK CELL VIOS Thermo Scientific 12-5...

  • Page 182: Reading Basic Parameters

    2 bytes / hexadecimal inactive) with the format xx Example of unit status and (error) status control loops (for details see error messages) All values have two decimal places. HERA 160i LK / 250i LK CELL VIOS 12-6 Thermo Scientific...

  • Page 183: Reading Internal Function Parameters

    *6) Send the next 7 entries. Set reading pointer automatically to next new entry, read up to 7 entries max. *7) Re-send entries of last telegram. Can be used after communication error has occurred. HERA 160i LK / 250i LK CELL VIOS Thermo Scientific 12-7...

  • Page 184: Error Memory Structure

    Therefore, a response consists of 1+(10 x 2) = 21 data bytes plus separator. A data set always delivers the date, the time, the faulty control loop, the device status, and the error message. HERA 160i LK / 250i LK CELL VIOS 12-8 Thermo Scientific...

  • Page 185: Error Memory Data Set Structure Scheme

    Therefore, the following information is transmitted in this data set: • Created on 1 January 2006 at 15:55:34 hrs. • A device error has occurred and the set temperature is too high. HERA 160i LK / 250i LK CELL VIOS Thermo Scientific 12-9...

  • Page 186: Overview Of The Possible Error Messages In Hex Coding

    0x0002 Actual value high 0x0004 Actual value low 0x0008 Actual value not plausible 0x0010 Calibration values too high/low control loop error status 0x0001 Sensor breakage 0x0002 Actual value high HERA 160i LK / 250i LK CELL VIOS 12-10 Thermo Scientific...

  • Page 187: O2 Control Loop And Water Level

    Set the data logger pointer to the oldest entry and output the first data sets. Query: ?:2401:00::cc<CR> Output of the following data sets, the reading pointer automatically moves  gradually from the older entries to the current entries. Query: ?:2402:00::cc<CR> HERA 160i LK / 250i LK CELL VIOS Thermo Scientific 12-11...
  • Page 188 First data set !:2400:e0:010b01060f3700000177002800d4000011 (consisting of 32 byte ASCII characters) Second data set 0b01060f3800000172003200d20352..:80 (beginning of the second data set after 32 bytes of the first data set) HERA 160i LK / 250i LK CELL VIOS 12-12 Thermo Scientific...

  • Page 189: Data Logger Data Set Structure Scheme

    • the temperature is 37.5°C, • gas concentration 4.0% CO , 21.2% O Note Example of a code: For an example of a code, please refer to the end of this chapter. HERA 160i LK / 250i LK CELL VIOS Thermo Scientific 12-13...

  • Page 190: Overview Of Possible Event Entries In Bit Coding Overview Of Event Entries, Part I

    O (2 bytes each) 0x50 Start auto-start Status / error registration for temperature, CO and rH 0x51 auto-start successfully completed Current actual values for temperature, CO and rH HERA 160i LK / 250i LK CELL VIOS 12-14 Thermo Scientific...

  • Page 191: Overview Of Event Entries, Part Ii

    2nd byte: day of entry  3rd byte: month 4th byte: year 5th byte: hours 6th byte: minutes 7th and 8th byte: device status 9th to 16th byte: various data on the event HERA 160i LK / 250i LK CELL VIOS Thermo Scientific 12-15...

  • Page 192: Functions For Data Logger Queries

    // calculate checksum: inverted XOR of all bytes // without checksum and <CR> for (i = 0; i < 11; i ++) bcc = (bcc^string[i]); // copy checksum string[11] = hexa(bcc/16); string[12] = hexa(bcc%16); HERA 160i LK / 250i LK CELL VIOS 12-16 Thermo Scientific...
  • Page 193 // Negative number?  if (z < 0) { // set algebraic sign b[0] = ‘-’;l = 1; // convert value z = 0xffffffff-z+1; } // store decimal place HERA 160i LK / 250i LK CELL VIOS Thermo Scientific 12-17...
  • Page 194 // set data logger to beginning and read if (!GetTele) { send_telegramm („2400“); } else{ // read additional data sets send_telegramm („2401“); } len = get_telegramm (buffer); // no telegram received HERA 160i LK / 250i LK CELL VIOS 12-18 Thermo Scientific...
  • Page 195 (status & INFO_ERROR){ str_cpy (&excelstring[len], „Error active;“, 13);  len += 13; } else{ // query all device errors (see “Overview of Possible Event Entries in Bit Coding Overview of Event HERA 160i LK / 250i LK CELL VIOS Thermo Scientific 12-19...
  • Page 196 += num_2_str (SollO2, &excelstring[len]); excelstring[len ++] = ‘;’; len += num_2_str (SollrH, &excelstring[len]); excelstring[len ++] = ‘;’; excelstring[len] = ‘\n’;  len += 1;  WriteFile (FileHandle, excelstring, len); break; HERA 160i LK / 250i LK CELL VIOS 12-20 Thermo Scientific...

  • Page 197: Program Heracell Vios 160I Lk / Heracell Vios 250I Lk

    Data Communication Program HERACELL VIOS 160i LK / HERACELL VIOS 250i LK // query the remaining events from here case EVENT_FORMAT_DATALOG: WriteFile (FileHandle, excelstring, len);  WriteFile (FileHandle, „Data logger erased;\n“,20);  break; case EVENT_POWER_ON: // update of set values...

  • Page 198: Installing The Heracell Vios 160I Lk & Heracell Vios 250I Lk

    Data Communication Program HERACELL VIOS 160i LK / HERACELL VIOS 250i LK • Reading and archiving error messages (error logger). The data sets are stored in the meta format *.CSV. • Reading and archiving event entries (data logger). The data sets are stored in the meta format *.CSV.
  • Page 199 Data Communication Program HERACELL VIOS 160i LK / HERACELL VIOS 250i LK HERA 160i LK HERA 250i LK CELL VIOS CELL VIOS Operating the & User menu structure: The user surface is divided into two main menus: • MAIN with two functional elements: •...
  • Page 200 Data Communication Program HERACELL VIOS 160i LK / HERACELL VIOS 250i LK Function of the user menu: PRESETTING The submenu PRESETTING is used to set the transmission speed and to select the serial port. 1. Select a transmission speed within the range of 9,600 to 115,200 baud.
  • Page 201 Data Communication Program HERACELL VIOS 160i LK / HERACELL VIOS 250i LK TEST COM The submenu TEST COM is used for testing the communication connection with the settings defined in the submenu PRESETTING. 1. Example of a query for the currently measurable temperature values of the incubator: •...
  • Page 202 Data Communication Program HERACELL VIOS 160i LK / HERACELL VIOS 250i LK DATE & TIME The submenu DATE & TIME is used for adjusting date and time to the desired time zone. 1. Data in the two text boxes must be entered in the format DD.MM.YY (day, month, year).
  • Page 203 Data Communication Program HERACELL VIOS 160i LK / HERACELL VIOS 250i LK The data sets can be stored in the metaformat *.CSV. 1. Read the data sets: • Press the READ key. 2. The progress of the data transmission is indicated in the three text boxes: •...
  • Page 204 Data Communication Program HERACELL VIOS 160i LK / HERACELL VIOS 250i LK PASSWORD The submenu PASSWORD can only be accessed by the service personnel of Thermo Fisher Scientific. HERA 160i LK / 250i LK CELL VIOS 12-28 Thermo Scientific...

  • Page 205: Chapter 13 Device Log

    Device Log Please list carried out works here: Device type: Part No.: Serial number: Service number: Location: Operator’s note: Work carried out: Comments: Date Signature HERA 160i LK / 250i LK CELL VIOS Thermo Scientific 13-1...
  • Page 206 Device Log HERA 160i LK / 250i LK CELL VIOS 13-2 Thermo Scientific...

  • Page 207: Chapter 14 Contact Information Thermo Scientific

    Contact Information Thermo Scientific Overview of the Thermo Fisher international sales organisations  postal address Germany: Thermo Electron LED GmbH Robert-Bosch-Straße 1 D - 63505 Langenselbold Enquiries from Germany: Phone Sales 0800 1 536376 Phone Service 0800 1 112110 Fax Sales/Service 0800 1 112114...
  • Page 208 Contact Information Thermo Scientific Enquiries al address USA: Thermo Scientific 275 Aiken Road Asheville, NC 28804 Enquiries from USA/Canada Sales: +1 866 984 3766 Service+1 800 438 4851 Enquiries from Latin America Sales: +1 866 984 3766 Service:+1 866 984 3766 Enquiries from Asia:...
  • Page 209 Contact Information Thermo Scientific Germany: Postal Address Germany: Thermo Electron LED GmbH Robert-Bosch-Straße 1 D - 63505 Langenselbold Phone Sales Toll free 0800 1 536 376 or +49 6184 90 6940 Service Toll free 0800 1 112110 or +49 6184 90 6940...
  • Page 210 Contact Information Thermo Scientific HERA 160i LK / 250i LK CELL VIOS 14-4 Thermo Scientific...

  • Page 211: Chapter 14 6-Door Gas Tight Screen For Cell Locker

    6-door gas tight screen for C ELL LOCKER ELL LOCKER Note Performance Change Due to the design of the 6-door gas tight screen the performance of the incubator changes, see Technical Data. HERA 160i LK / 250i LK CELL VIOS Thermo Scientific...

  • Page 212: Installation Of The Shelving

    Before the perforated shelf with unseparated guiding rails can be placed, the guiding rails (2) must be slotted in. The guiding rails can be removed, if necessary. Place the perforated shelf with the separated guide rails (1) and middle openings in the lower position. HERA 160i LK / 250i LK CELL VIOS Thermo Scientific...
  • Page 213 Place the perforated shelves, so it snaps in behind the rail. The metal tab underneath the shelf needs to be fixed behind the rail for door closure. Close the 6-door gas tight screen. HERA 160i LK / 250i LK CELL VIOS Thermo Scientific...
  • Page 214 6-door Gas Tight Screen for Cell Locker Installation of the Shelving HERA 160i LK / 250i LK CELL VIOS Thermo Scientific...
  • Page 215 Operating Instructions Cell Locker 50155007 Revision C May 2021 Cell Locker 50155007_C 27.05.21 Thermo Fisher Scientific...
  • Page 216 Trademarks ELL LOCKER ® is a registered trademark of Thermo Scientific. Thermo Scientific is a brand owned by Thermo Fisher Scientific, Inc. All other trademarks mentioned in the operating instructions are the exclusive property of the respective manufacturers. Thermo Electron LED GmbH Robert-Bosch-Straße 1...
  • Page 217 Table of Contents Table of Contents Chapter 1 General notes ........... . . 2 1.1.

  • Page 218: Chapter 1 General Notes

    Chapter 1 General notes 1. General notes 1.1 Explanation of safety information and symbols Warning Indicates a hazardous situation which, if not avoided, could result in death or serious injuries. Caution Indicates a hazardous situation which, if not avoided, could result in minor or moderate injuries. Notice Indicates a situation which, if not avoided, could result in damage to equipment or property.

  • Page 219: 1.2 Safety Notes

    Chapter 1 General notes 1.2 Safety notes The C ELL LOCKER may only be used by instructed personnel. The present operating instructions, applicable safety data sheets, hygiene guidelines and the corresponding technical rules issued by the operator shall be used to create written procedures targeted at personnel working with the subject matter device, detailing: •...

  • Page 220: 1.5 Intended Purpose

    Chapter 1 General notes 1.5 Intended Purpose 1.5.1. Correct Use The C ELL LOCKER is intended for use in laboratory equipment, which among others is used for the preparation and cultivation of cell and tissue cultures for research applications. The maximum Autoclave temperature is 15 minutes at 121°C (250 °F) with fast exhaust. 1.5.2.

  • Page 221: Chapter 2 Delivery Of The Device

    Chapter 2 Delivery of the device 2. Delivery of the device 2.1 Scope of delivery Description Pcs. ELL LOCKER Membrane filter Work tray with cover Consumables kit Operating instructions Summarized safety instructions 2.2 Acceptance inspection After the C ELL LOCKER has been delivered, check the delivery immediately for: •...

  • Page 222: Chapter 3 Description Of The Device

    Chapter 3 Description of the device 3. Description of the device 3.1 Description The C ELL LOCKER consists of a polycarbonate chamber with two membrane filters, which are permeable to water vapor and gas, but are impermeable to microorganisms. ELL LOCKER Fig.

  • Page 223: 3.2 First Use

    Chapter 3 Description of the device 3.2 First use When using the first time, clean and disinfect the C ELL LOCKER Note Clean the C ELL LOCKER before inserting the membrane frame with filter. Warning The C ELL LOCKER must be removed before the incubator is sterilized. 3.3 Operation The C ELL LOCKER...
  • Page 224 Chapter 3 Description of the device Cover can be attached to work tray with the Allen key, allowing protected transport. with attached transport cover without transport cover with cover to remove C without cover to remove cultures with single ELL LOCKER with cultures door access Note:...

  • Page 225: Chapter 4 Cleaning And Disinfection

    Chapter 4 Cleaning and disinfection 4. Cleaning and disinfection Before starting any work, the following safety hints should be followed: Warning Health hazard! The surfaces of the work space may be contaminated. Contact with contaminated cleaning liquids may cause infections. Disinfectants may contain harmful substances. When cleaning and disinfecting, always observe the safety instructions and hygiene guidelines.

  • Page 226: 4.2 Wipe/Spray Disinfection

    Chapter 4 Cleaning and disinfection 4.2 Wipe/spray disinfection Caution Alcoholic disinfectants! Disinfectants having a alcohol content of more than 10% may form, in combination with air, easily combustible and explosive gas mixtures. When using such disinfectants, avoid open flames or exposure to excessive heat during the entire disinfection process.

  • Page 227: 4.3 Autoclaving

    Chapter 4 Cleaning and disinfection 4.3 Autoclaving The C ELL LOCKER can be autoclaved up to 15 minutes at 121°C with fast exhaust. Autoclaving at higher temperature is not recommended since the material cannot withstand higher temperature and its composition would be negatively affected. Note Remove the membrane frame with filter before autoclaving the C ELL LOCKER...

  • Page 228: Chapter 5 Service

    Chapter 5 Service 5. Service Any service work should be recorded. 5.1 Membrane filter replacement Periodically check the membrane frame filters and at least once a year replace them. Warning Health hazard! Wear safety gloves! Wear breathing protection! 1. Pull out the work tray and take it out. 2.
  • Page 229 Chapter 5 Service 4. Unpack the new membranes. 5. Insert the new membrane frame and press firmly in the radial direction. 6. Visually inspect from the inside, whether the gasket lip is correctly seated, if necessary press from the outside. 7.

  • Page 230: 5.2 Cover Gasket Replacement

    Chapter 5 Service 5.2 Cover gasket replacement Inspect the cover gasket periodically, e.g. monthly, for integrity, no damage and replace as needed. 1. Pull out the work tray and take it out. 2. Remove cover gasket from the notch. 3. Insert a new gasket in the notch and take care that the gasket lip is outside. Warning Contamination hazard! Damaged or improperly mounted membranes can contaminate the user or the environment! 4.

  • Page 231: Chapter 6 Spare And Wear Parts

    Chapter 6 Spare and wear parts 6. Spare and wear parts Description Material-No. Membrane frame‘s 50153148 Cover seal (6 pieces) 50156433 Work tray, stainless steel 50155808 Work tray, copper 50155809 Consumables kit 50161834 ELL LOCKER 50156434 Cover Only original spare parts and accessories that have been approved by Thermo Fisher Scientific used, third-party spares without Thermo Fisher Scientific approval void the limited warranty.

  • Page 232: Chapter 7 Disposal

    Chapter 7 Disposal 7. Disposal Warning Contamination hazard! The device may have been used for treating and processing infectious substances. Therefore, the device and device components may have been contaminated. Prior to disposal, all device components must be decontaminated! Clean the unit components thoroughly, then disinfect ,decontaminate or autoclave them (depending on application).

  • Page 233: Chapter 8 Technical Data

    Chapter 8 Technical Data 8. Technical Data Description Unit Value Outer dimensions (W x H x D) 185 x 156 x 380 Inner space volume approx. 5.4 Weight Loading capacity per work tray Operating temperature °C / °F 4 - 55 / 39 - 131 Autoclave cycles max.

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