• Keep these operating instructions close to the device so that safety instructions and important information are always accessible. Should you encounter problems that are not detailed adequately in these operating instructions, please contact Thermo Scientific immediately for your own safety. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
Chapter 1 General notes 1.5 Warranty Thermo Scientific warrants the operational safety and functions of the CO incubator only under the condition that: • the device is operated and serviced exclusively in accordance with its intended purpose and as described in these operating instructions, •...
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Electric shock! Hot surfaces! Fire hazard! Explosion hazard! Suffocation hazard! Symbols on the device CE conformity mark: confirms conformity according to EU Guidelines VDE test mark Mark of conformity USA/Canada Observe operating instructions! Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
• are pyrotechnical substances. This product is intended for General Laboratory Use. It is the customer’s responsibility to ensure that the performance of the product is suitable for its specific use or application. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
• Safety data sheets of the gas suppliers with regard to the particular characteristics of CO and N • Principles of good microbiological proceedings, notice of the trade association of the German chemical industry. For other countries, the applicable national regulations are binding. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
• Refer to the Appendix titled “BASIC RULES OF SOUND MICROBIOLOGICAL ENGINEERINGPRACTICE”. • If the equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may be impaired. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
• For cleaning these device components, use only oil- and grease-free lubricants. • Keep all connections and components of the oxygen system free from substances that contain oil, grease, or lubricant! Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
CO -incubator. • The tempering of defined substances or materials with higher moisture content can result in increased condensation in the chamber. Measures on must be observed. Page 69 Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
• possible damage If components are missing or damage is found on the device or the packaging, esp. damages caused by humidity and water, please contact the line-hauler as well as the Technical Support of Thermo Scientific immediately. Caution Risk of injury Should sharp edges have formed in damaged areas or elsewhere on the device, take all necessary precautions to protect personnel handling the incubator.
(optional) connecting hose set, gas monitoring (optional) *1) O sensor with gas humidification set is packed in a separate carton and placed into the work space during transport. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
24 mm Allen wrench 2 mm for blower wheel Allen wrench 3 mm for blower wheel cover Operating instructions (CD) Summarized safety instructions Water pump Support bracket (Only for stacked units needed) Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
*2) For bottle turning devices, the version with split shelves is not available. *3) Each level of a bottle turning device is packed in a separate box. The boxes (1 to 4) are delivered with the device. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
• Transient surges must lie within the range of levels that normally occur in the power supply system. The impulse withstand voltage based on surge category II of IEC 60364-4-444 shall be applied at the nominal voltage level. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
To protect the CO incubator against contamination, use a floor stand even if the device is installed near the floor. The height of the floor stand should not fall below 200 mm. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
Chapter 3 Installation of the device Figure 1. Device dimensions Note Accessibility of the devices: To ensure the accessibility for care and maintenance works, keep larger side and rear distances. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
After transport or storing under humid conditions a drying-out process must be performed. During the drying-out process the equipment cannot be assumed to meet all the safety requirements of the IEC 61010-2-010 standard. The drying-out period is 2 hours. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
240i only: For thermal separation, an adapter plate [3] can be placed between the two CELL devices. Heracell 150i-240i with Heracell 150i -150i without Heracell 240i-240i with stacking adapter stacking adapter stacking adapter Figure 3. Stacking Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
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• Place the device to be stacked with the device stands [6] onto the stacking elements [7] at the top of the adapter plate. The devices are secured to one another by their own weight. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
• 3-segment gas-tight screen (the gas-tight screen is installed in place of the glass door) • door hinge replacement on outer door and glass door (RH / LH door opening) • lockable outer door • split inserts • infrared (IR) sensor • PCB for USB interface Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
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• lockable outer door • split inserts • infrared (IR) sensor • PCB for USB interface Note Modifications: Retrofitting and modifications must only be performed by the Technical Service of Thermo Electron LED GmbH. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
20. Access port with plug 21. Glass door seal, replaceable 22. Operating panel (touchscreen) 23. Door handle ® 4.2. Rear view HERACELL 150i GP ® Figure 5. Rear view HERACELL 150i GP Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
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(independent of device type) 14. Distributor connection for looping through the O gas supply for up to 3 additional devices (independent of device type) 15. Diagram: Gas connection CO and O with optional gas monitoring Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
6. Diagram: Gas connection CO and O with optional gas monitoring. ® 7. HERA 150i GP with looped-through gas supply; therefore device must only be equipped CELL with combined gas connection. (optional) CO and O Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
4. Measuring cell with blower wheel and sensors 5. Door switch 6. Oxygen sensor (optional) 7. Receptacle for bottle turning device (optional) 8. Measuring opening 9. Plug for bottle turning device (optional) Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
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22. Latch, glass door 23. Support hook for shelf 24. Pressure compensation opening with insert 25. Access port with plug 26. Glass door seal, replaceable 27. Operating panel (touchscreen) 28. Door handle Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
O without gas monitoring 5. Diagram: Gas connection CO and O without gas monitoring 6. O gas cylinder 7. CO gas cylinder 8. Secondary CO gas supply B with optional gas monitoring Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
(independent of device type) 15. Diagram: Gas connection CO and O with optional gas monitoring ® Looped-through gas supply for HERA 240i GP: CELL ® Figure 9. Looped-through gas supply HERACELL 240i GP Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
55 °C. The principle of air jacket heating and the additional, separate heating of the outer door and glass door/gas-tight screen minimize the risk of condensate formation at the side walls, at the ceiling of the work space, and at the glass door / gas-tight screen. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
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Caution Risk of an electric shock Fill the water reservoir just up to the max. level. Overfilling could cause a hazard of the user or a malfunction of the device (short circuit). Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
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The O -content of the work space atmosphere can be controlled depending on the configuration of the sensor within a range of 1 % to 21 % and 5 % to 90 %. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
The outer door can only be closed after the glass door has been latched properly. Note Versions with gas-tight screen: For devices with the optional gas-tight screen, the door switch function described above is activated when the outer door is opened. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
Their measured values are compared to the selected set values. Based on this data, the control system controls heating and CO supply. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
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• the alarm relay responds If the error message is accepted, the display shows the Over temperature icon to indicate the response of the thermal protection, and the temperature display is highlighted in red. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
Chapter 4 Description of the Device 4.9. Supply Interface Figure 12. Supply interface All supply connections are installed in the supply interface (control box [1]) in the rear of the device. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
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The connection to the power supply system is established by connecting a cable with plug for IEC connector [9] to the socket [8]. The receptacle for the two device fuses is integral to the socket. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
4.10. Work Space Components Figure 13. Work space components The work space of the incubator has only a minimum of surface, thereby supporting both the prevention of contamination and easy, effective decontamination. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
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The components of the shelf system can be removed easily so that only the easily treatable, surface-reduced interior container [1] remains in the work space for cleaning and manual disinfection works. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
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• work space temperature • CO concentration • O concentration • relative humidity Advantage of the gas-tight screen: The cross sections of the openings are smaller when samples are accessed. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
[2] that issues an alarm message at the display and an audible signal when the water falls below the minimal level. The embossings [3] in the water tray are used as indicators for the maximal level. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
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The outer door of the device is also heated. The heat radiated onto the interior glass door/gas-tight screen prevents condensate formation. The work space of the device always remains visible, despite high humidity. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
A sealable access port allows cables, hoses or additional sensors to be routed into the work space of the device. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
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The support rails [1] of the shelf system are perforated every 42 mm so that the support hooks [8] can be inserted variably for any culture container size required. The shelves [2] have an integrated tilt protection and withdrawal stop. For details of the shelf system, see “Start-up” page Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
240i GP may be equipped with up to four separate bottle turning devices. Each unit CELL consists of a roller insert [6] with a drive roller [4] and four idle rollers [7] that are also rotated due to the transmission function of the inserted cylinders [5]. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
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The rotating speed of the bottle turning devices is set depending on two parameters: • Diameter (of the cylinders used), • drive roller set value. The value is determined using a diagram (see “Installing the Optional Bottle Turning Devices HERACELL® 240i GP” page 54). Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
Chapter 4 Description of the Device 4.10.9. Water pump Figure 17. Water pump Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
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5. Let the water run into the bucket [2] through gravity. 6. Wipe out the rests of the water at the floor plate of the water reservoir. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
• work space surfaces, • work space seals and gaskets, • glass door/gas-tight screen. Note Decontamination: “Cleaning and Disinfection” For details about the cleaning and disinfection of the device (see page 124 Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
Tools are not required for the installation of the shelf system. The support rails are secured using spring pressure. After the support hooks have been inserted into the rail, the shelves are pushed onto the support hooks. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
[5] and [2]. 2. Clamp the lockspring [3] behind the upper embossing [1]. 3. To remove the support rails, pull the lockspring tab down out of the embossing and remove the rail. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
2. Rotate the adjustable device stands using the supplied 24 mm wrench until the shelf is positioned horizontally in all directions. Perform the adjustment of the device stands from left to right and from rear to front. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
1. Install hose [2] to the sleeve of the gas humidification and then to the sleeve [3] of the device-integral oxygen or nitrogen supply line. 2. Place gas humidification immediately to the rear panel of the device and align it parallel to the rear panel. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
• Set the desired speed at the Y axis. Read the corresponding set value (in %) from the X axis at the point of intersection of the Y value with the specific graph. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
• Bottle diameter: 56 mm • Desired speed: 3.5 rpm • Determined set value: 48 % In the dialog box SPEED BOTTLE TURN. DEVICE, the value 48 % is set (see “Options” page 102). Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
2. Then, position the lower rod guide [5] of the center strut into the hole [6] in the work space floor and remove spring tension. 3. The center strut is stabilized by the spring pressure. Note Configuration with split inserts: For versions with split inserts, bottle turning devices cannot be installed. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
Set the gas supply to a range between 0.8 bar min. and 1.0 bar max. and make sure that this pressure setting cannot be changed! 5.7.1. Installing gas pressure hoses Figure 24. Installing gas pressure hoses Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
The pipe of the pressure compensation opening must not be extended or redirected. 5.7.2. Installing devices without the optional gas monitoring system Figure 25. Gas connection without gas monitoring system Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
The gas supply from the gas supply system to the device is established by the supplied flexible gas pressure hoses. Devices with CO connections that are equipped with the optional gas monitoring system are connected according to the connection diagram [7]. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
(optional) Figure 27. Combined connection with gas monitoring system (optional) The gas supply from the gas supply system to the device is established by the supplied flexible gas pressure hoses. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
Do not use damaged components when connecting the device to the power supply! The device must be connected only to a correctly installed and grounded power supply source: • Fusing T 10 A • Circuit breaker G 16 Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
Make sure that power sockets remain freely accessible at all times! To allow a rapid disconnection of power in case of an emergency, make sure that power outlets remain freely accessible at all times! Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
3. Connect the second connector to an unassigned serial port COM 1/COM 2 etc. at the PC. 4. Turn the PC on. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
Note Skilled work: Thermo Scientific warrants the operational safety and the operativeness of the device only if installation and repairs are performed properly. The connection of the device to an external alarm system must only be carried out by adequately...
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To avoid overloading and damaging the alarm contact, check the electrical interfacing parameters of the alarm-receiving system for compatibility with the alarm-relay specifications given above. Note Blackout: If the alarm relay reports a blackout, no display appears and the switches do not blink. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
CO -incubator in the stack. 3. Connect the connector [5] of the alarm system connecting cable to the interface [6] at the control box at the rear panel of the device. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
• Disinfecting and cleaning the incubators work space with fuzz-free materials. • Disinfect the work space according to the operator-specified hygiene guidelines. • Don‘t use any explosive disinfectants. When using alcohol as a disinfectant, attend the national regulations. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
8. The temperature control adjusts the temperature to the selected set value, humidity rises. 9. When temperature and relative humidity are constant, the automatic adjustment of the measuring system is performed. 10. The CO control supplies the set amount of CO Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
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-incubator to tilt. When the shelves are being drawn out, ultimately destroying the samples. To avoid overloading the incubator or its shelving be sure to observe the sample weight limits specified in chapter 12. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
Make sure to prevent Contamination when operation is interrupted. 1. Remove all samples an brought in tools from the device, then clean and disinfect them. 2. Clean and disinfect the internal space. 3. Eliminate any debris. 4. Decontaminate the device. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
− The control loop sensors pass the heat-up phase (see “Heat-up Phase of the Control Loop Sensors” page 76). • To turn the device off: Press the power switch; the switch illumination goes off. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
[5] (optional) • Contra-con key [2] • Menu key [4] • Auto-start key [6] Note Extended pressure-sensitive area: To accept a failure message, the entire touchscreen can be used as a pressure-sensitive area. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
5. Key for opening the menu navigation 6. Key for starting the auto-start routine 7. Actual value CO 8. Set value CO 9. Temperature actual value 10. Temperature set value 11. Current date and time Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
7. Key for opening the menu navigation 8. Key for starting the auto-start routine 9. O display 10. O actual value 11. O set value 12. Temperature actual value 13. Temperature set value 14. Current date and time Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
Direct access to the control loop settings: Temperature, CO set value and bottle turning device (optional), • Start of the device routines contra-con or auto-start, • Navigation through the submenus for device configuration. Figure 34. Menu structure overview Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
When the device has been switched on, the control loop sensors pass through a heat-up phase of varying duration during the start process: Temperature control loop 10 s control loop 10 s control loop with IR measuring cell 3 min control loop 5 min Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
To save changed values, press the Enter key. Resetting the settings: Unless a user action (contact with the pressure-sensitive areas and keys) occurs within 30 seconds, the system automatically exits the menu and the most recently confirmed setting defaults. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
TEMPERATURE DISPLAY − The Temperature menu is displayed. 2. To exit the Temperature menu: − Press the key [2]. 3. To set the temperature set value: − Press the key [3]. SET VALUE Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
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4. To accept and save the set value: − Press the key [5]. ENTER − The system returns to the main menu. The temperature displays shows the actual value currently measured in the work space. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
2. To exit the CO menu: − Press the key [2]. 3. To set the CO set value: − Press the key [3]. SET VALUE To increase the set value: Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
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For set values of 21 % O , the O control is switched off and error monitoring is disabled as well. The control loop error monitoring system (optional) is enabled. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
2 DISPLAY − The O menu is displayed. 2. To exit the O menu: − Press the key [2]. 3. To set the O set value: − Press the key [3]. SET VALUE Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
When temperature and relative humidity have reached constant values, the CO measuring system is automatically adjusted to these values, and the work space is supplied with the preset quantity of CO Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
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Actual value below set value (excessive deviation) • Set value not plausible • Calibration values too high or too low • Sensor communication failure • Sensor parameter not plausible • No communication with LM 75 Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
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If the auto-start routine is to be run dry (without water in the floorpan of the work space), the water “Options” page 102 level sensor must be disabled prior to its start (see Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
2. Fill the floorpan of the work space with a sufficient quantity of water. The filling level must not exceed the upper filling level mark. 3. Set the set values for temperature, CO and O on the touchscreen. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
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The auto-start routine is restarted automatically if the routine is canceled due to one of the following events: • The glass door is opened, • the outer door is opened on a device with optional gas-tight screen, • the power supply is interrupted. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
1. To resume the auto-start routine: − Press the key [1]. BACK − The system returns to the status display, and the auto-start routine is resumed. 2. To cancel the auto-start routine: − Press the key [2]. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
1. To enter the default 0000: − Press the corresponding numeric keys. − The number combination is displayed hidden in the input box [1]. 2. To delete the input numbers: − Press the key [2]. DELETE Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
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The user-defined code can be changed as often as required using the same procedure: • Activate the recoding function by entering the valid code. • Enter the new code and confirm it. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
4. The system returns to the Date/Time menu. 5. Enter the time accordingly. 6. To accept and save the input value: − Press the key [3]. ENTER − The system returns to the Date/Time menu. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
3. The value change appears in the display [1]. − To accept and save the change: − Press the key [3]. ENTER − The system returns to the Settings / Setup menu. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
[2]. 3. The value change appears in the display [1]. 4. To accept and save the change: − Press the key [3]. ENTER − The system returns to the Settings/Setup menu. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
5. To activate the new settings: − Browse back to the main menu. − Wait for approx. 10 seconds and perform a reset by switching the device off and on using the power switch. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
[2]. 3. The new language appears in the display [1]. 4. To accept and save the selection: − Press the key [3]. ENTER − The system returns to the Settings menu. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
2. To reduce the number of days: − Press the key [2]. 3. The value change appears in the display [1]. 4. To deactivate the reminder interval: − Set the value to − Press the key [2]. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
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Service interval: Request Service icon is displayed. After the routines have been run successfully, the reminder messages are hidden. Factory settings: Contra-con decontamination routine 90 days Auto-start routine Service interval 365 days Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
2. To browse backward in the list: − Press the key [3]. PREVIOUS 3. To exit the display: − Press the key [4]. − The system returns to the Event Logger menu. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
[2]. 3. The value change appears in the display [1]. 4. To accept and save the change: − Press the key [3]. ENTER − The system returns to the Event Logger menu Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
[3]. − The system returns to the Event Logger menu. Note Troubleshooting: For a detailed overview of causes for errors and their correction, please refer to the end of this chapter! Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
1. To toggle between two states [1]: − Press the key [4]. − Press the key [2]. 2. To accept and save the change: − Press the key [3]. ENTER − The system returns to the Options menu. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
1. To toggle between two states [1]: − Press the key [4]. − Press the key [2]. 2. To accept and save the change: − Press the key [3]. ENTER − The system returns to the Options menu. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
The conversion to gas-tight screen causes a change of the control parameters. Failure to set the gas-tight screen function in accordance with the actually installed door version may impair the incubation performance. Figure 54. Gas tight screen Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
1. To toggle between two states [1]: − Press the key [4]. − Press the key [2]. 2. To accept and save the change: − Press the key [3]. ENTER − The system returns to the Options menu. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
“Bottle turning device for HERACELL® 240i GP (optional)” page 45). The drive roller of a bottle turning insert is activated as soon as a value >0 for the speed has been entered and saved. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
− The system returns to the Bottle Turning Device menu. Note Quick access to bottle turning device levels: To directly access the Bottle turning device submenu, touch the Bottle turning device icon in the main menu. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
[2]. 2. The value change appears in the display [1]. 3. To accept and save the setting: − Press the key [3]. ENTER − The system returns to the Options menu. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
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The gas monitoring system remains active even when the O control is switched off or the O sensor is disabled. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
The Icon Description dialog box [1] explains the meaning of the individual icons. Figure 59. Icon description overview • To exit the display: − Press the key [2]. − The system returns to the Configuration menu. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
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This indicator shows that the routine service is due. The display of the icon is controlled by the time entry in the dialog box and it appears after the reminder message has been REMINDER INTERVAL confirmed. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
• an audible alarm sounds and the alarm relay switches, • an error message is displayed, • an entry is written into the error table, • an entry is written into the event list. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
3. To break off the input: − Press the key[4]. BACK The system returns to the Configuration menu. 4. To confirm the input: − Press the key [3]. ENTER − The system returns to the Configuration menu. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
This menu shows the unit software versions in the display [1]. Figure 62. Software versions • To exit the display: − Press the key [2]. − The system returns to the Configuration menu. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
(press onto screen at left of upper diagram frame) to the end (release at right of lower diagram frame). − Press any position the marked rectangle area [2]. The section is now enlarged. within Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
• Aflashing warning triangle [1] is displayed in the main menu; the value indicators are no longer updated, • The detected error is listed in the error table, • The event is entered into the event display. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
• The Error dialog box [2] appears and the detected error is displayed, • The audible alarm is switched off. 2. To exit the error display: • Press the key [3]. • The error message disappears. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
If the overtemperature protection mechanism is re-activated although the potential fault causes (see “Error message overview:” ) have been eliminated, take the device out of service and contact the technical service center. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
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System - Safety Temperature signal Reset device. circuit active When failure is plausibility doubtful listed several times, contact Service. Failure bottle Communication Contact Service turning device between bottle turning device and main board faulty Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
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Failure communi- Measuring cell can- Contact Service cation not communicate with mainboard Failure LM75 LM75 sensor cannot Contact Service communicate with mainboard Failure EEPROM NV-Ram sensor faulty Contact Service sensor Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
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Gas cylinder A Gas cylinder A is Replace gas empty empty cylinder A Gas cylinder B Gas cylinder B is Replace gas empty empty cylinder B Failure EEPROM NV-RAM sensor error Contact Service sensor Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
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2 If failure is detectec, a special sample protection controlling will be enabled. To indicate the start of this routine, the icon overtemperature is displayed. The routine will be disabled after a power reset. 3 After changing the set value, error time will be set on 159min, after door opening on 45 min.(O on 159 min.). Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
8. Clean the work space and wipe the device dry. 9. Until the device is shut down, the work space must be continuously ventilated. Leave the glass door and the outer door open and secure them in this state. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
The following disinfection procedures are compatible with the device: Wipe/spray disinfection: The wipe/spray disinfection is used as the standardized manual disinfection procedure for the device and for all accessories. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
Prior to cleaning and disinfection work, disconnect the device from the power supply! • Turn the device off using the power switch. • Unplug the power connector and protect it against accidental reconnection. • Check to see if the device is deenergized. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
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Notice Moisture-sensitive components! Do not spray disinfectant onto the CO sensor in the measuring cell baseplate or onto the sensor. 4. Allow the disinfectant to react as specified by the manufacturer. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
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3. Reinstall the shelf system and the removed components. Note Functional check: After the installation, check to see if the blower wheel is securely attached to the axle and if it can rotate freely, then secure cover using the screws. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
During this routine, a hot and humid atmosphere at 90 °C with highly decontaminating effect is created in the work space. The effectiveness of the contra-con decontamination routine has been certified by independent institutes. Information on these tests is available from Thermo Scientific upon request.
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Make sure that: • all samples are removed from the work space, • all accessories are removed from the work space. Notice The contra-con decontamination routine must be performed every 3 months. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
When the remaining run time has elapsed to 0 hours, the device has reached the originally set working temperature again (e.g. 37 °C). The contra-con decontamination routine must then be stopped by pressing the appropriate key. Figure 66. Decontamination routine phases Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
− The contra-con-instruction menu [2] is displayed. 2. To exit the contra-con-instruction menu and contra-con: − Press the key [4]. − The system returns to the main menu. 3. To activate contra-con: Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
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− The run of the contra-con decontamination routine starts. − While the contra-con decontamination routine is running, the display shows the current status [5] and outputs the following information: • Temperature, • start time, • phase, • remaining run time. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
Stop dialog box is opened STOP as a security request. The routine can now be ultimately canceled or resumed. 2. To cancel contra-con: − Press the key [2]. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
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− If the decontamination routine is not canceled after this, cool-down to the set temperature occurs. 2. To cancel contra-con: − Press the key [2]. − The error message is displayed. − If the error message is confirmed, the system returns to the main menu. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
Opening door during contra-con decontamination: If the door will be opened and closed again whilst contra-con decontamination is running, the routine returns to a phase which will ensure a continuation without faults. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
Annual service: • Replace gas inlet filter. • Perform Technical Service inspection. Note Service contract: Thermo Scientific offer a device-specific service contact that comprises all test and service works required. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
• Clean the Incubator components thoroughly, then disinfect or decontaminate them (depending on application). • Fill in and attach a safety declaration with details on decontamination activities performed to the items that are to be repaired. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
Note Isothermal container: Thermo Scientific offer a device-specific service contact that comprises all test and service works required. Do not use a container filled with water as isothermal container as the evaporation of water will result in a lower temperature reading.
[1] in the rear panel of the device. 5. Close the doors. 6. Wait until the temperature value displayed on the measuring instrument has stabilized. 7. Calibrate the temperature control as described in “Temperature Calibration Procedure” page 140. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
Temperature calibration procedure Measurement example: • Temperature set value: 37 °C Reference temperature: 36.4 °C 1. Press TEMPERATURE DISPLAY [1]. − The temperature menu is displayed. 2. To exit the temperature menu: Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
• Portable IR readout instrument. The measuring sample is withdrawn through the sealable measurement opening of the glass door. The comparison measurement must be performed when the device is completely heated up. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
4. For devices equipped with the optional gas-tight screen, the measurement opening is located: ® − on HERA 150i GP [1] in the center gas-tight screen, CELL ® − on HERA 240i GP [2] in the left center gas-tight screen. CELL Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
4.0 % or more. Calibration should be performed with the CO set value designated for the work process (prospective work process value). 10.8. CO Calibration Procedure Figure 74. calibration procedure Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
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30 seconds. Value reset: If the value isn’t changed within the next 30 seconds, the system automatically exits the menu, and the most recently confirmed value is preserved. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
Procedure for gas supply gas inlet filter: 8. Connect the gas hose to the gas inlet filter sleeve and secure it using the hose clamp. Check to see if the gas hose is securely seated on the sleeve. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
3. Remove the faulty fuse from the fuse holder and install the new fuse. 4. Slide the fuse holder into the fuse compartment and press the holder on until the locking tabs are fully engaged. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
After installing the door seal, there may be a gap between the seal and the device. The gap may only appear on the hinge side and have a maximum size of 1mm. The described gap up to 1mm has no influence on the performance of the device. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
93 Constant humidity at 37 °C (low-humidity mode) % rH approx 90 Others Sound pressure level (DIN 45 635, Part 1) dB(A) < 50 Relative humidity of environment % rH max. 80 Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
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G 16 0.58 (230 VAC) Rated input 0.62 (120 VAC) 0.62 (100 VAC) EMC class 1 Determined on the basis of DIN 12880 for devices with standard equipment. See detailed information in calibration. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
Constant humidity at 37 °C (low-humidity mode) % rH approx 90 Others Sound pressure level (DIN 45 635, Part 1) dB(A) < 50 Relative humidity of environment % rH max. 80 Location elevation m ASL max. 2000 Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
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G 16 0.64 (230 VAC) Rated input 0.65 (120 VAC) 0.65 (100 VAC) EMC class 1 Determined on the basis of DIN 12880 for devices with standard equipment. See detailed information in calibration. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
The assigned COM port can be located in the Windows Device Manager/Ports dialog box, e.g. USB Serial Port (COM5), and is then defined as communication port in the “Program HERACELL® 150i/240i GP” page 176 HERACELL® 150i/240i GP program (see Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
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As soon as the Windows Hardware Detector has identified the USB port, the FIND NEW HARDWARE dialog box opens. WIZARD 1. Select the option. INSTALL FROM A LIST OR SPECIFIC LOCATION (ADVANCED) Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
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FINISH The interface transmission speed can be selected within the defined baud rates ® (9,600, 19,200, 38,400, 57,600 Baud) at the touchscreen of the HERA 150i/240i GP CELL (see “Settings” page 89). Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
= parameter address bb = amount of payload in this telegram (00 – ff) cc = checksum: Inverted XOR of all bytes without checksum and <CR> XXXX = bb payload bytes Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
8 digits 0010 Output date and time 17 bytes/decimal value in format [hours:minutes:seconds], xx:xx:xx;xx:xx:xx [day:month:year] 0011 Date 8 bytes/decimal value in format xx:xx:xx [day:month:year] 0012 Time 8 bytes/decimal value in format xx:xx:xx [hours:minutes:seconds] Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
O 2300 Up to 241 bytes/hexadecimal value, Read error memory (current errors) format see corresponding chapter 2301 Up to 241 bytes/hexadecimal value, Read error memory (prior errors) format see corresponding chapter Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
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Waiting period 1 0x08 Cool-Down Set tolerance band 0x09 Establish stable humidity 0x0A Await release Perform counter-voltage adjustment 0x0B Stop Waiting period 2 0x0C Determine offset 0x0D Read offset, check 0x0E Release 0x0F Stop Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
Example of a response: First data set: !:2300:fb:10b01060f372280000002: (with 21 bytes) Second data set:20b01060f38100001..:80 (beginning of the second data set after 21 bytes of the first data set and of the separator [1 byte]) Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
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• A device error has occurred and the set temperature is too high. 13.5.2. Overview of the possible error messages in hex coding Hex Code Description/Type 0x00 Temperature control loop 0x01 control loop 0x02 control loop 0x07 Water level 0x08 General device status Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
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Communication error (to sensor) 0x0040 Communication error (to cylinder changeover switch) 0x0080 No gas, cylinders A and B empty 0x0100 Measuring cell parameters implausible (EEPROM faulty) 0x0200 Cylinder A empty 0x0400 Cylinder B empty Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
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Communication error (to sensor) 0x0040 Gas cylinder changeover switch fails to communicate 0x0080 No gas, cylinders A and B empty 0x0200 Cylinder A empty 0x0400 Cylinder B empty Water level error status 0x0001 No water Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
Example of a response: First data set !:2400:e0:010b01060f3700000177002800d4000011 (consisting of 32 byte ASCII characters) Second data set0b01060f3800000172003200d20352..:80 (beginning of the second data set after 32 bytes of the first data set) Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
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• The temperature is 37.5 °C, • Gas concentration 4.0% CO , 21.2% O Note Example of a code: For an example of a code, please refer to the end of this chapter. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
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0x61 contra-con successfully completed Current actual values for temperature, and rh 0x62 contra-con completed with error Status/error registration for temperature, and rh 0x63 contra-con stopped manually Status/error registration for temperature, and rh Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
2. byte: day of entry 3. byte: month 4. byte: year 5. byte: hour 6. byte: minute 7. byte: device status 8. byte: device status 9. to 16. byte: various data on the event Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
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(&string[2], p, 4); // calculate checksum: inverted XOR of all bytes // without checksum and <CR> for (i = 0; i < 11; i ++) bcc = (bcc^string[i]); // copy checksum Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
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= 0; l = 0; // Negative number? if (z < 0) { // set algebraic sign b[0] = '-'; l = 1; // convert value z = 0xffffffff-z+1; Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
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Act.;rH Act.;Temp Set;CO Set;O Set;rH Set;\n", 85); // infinite loop while (1) // set data logger to beginning and read if (!GetTele){ send_telegramm ("2400"); else{ // read additional data sets send_telegramm ("2401"); Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
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++] = ':'; len += time_2_str (0, &excelstring[len]); excelstring[len ++] = ';'; switch (zahlenstring[i*SIZE_DATA2]){ case EVENT_STATUS: // check cyclic entries for device errors status = zahlenstring[6+i*SIZE_DATA2]*0x100+ zahlenstring[7+i*SIZE_DATA2]; if (status & INFO_ERROR){ Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
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// query all events here (see “Overview of possible event entries in bit coding” page 168) // cancel 0xFF indicates the end of the data logger case 0xFF: WriteFile (FileHandle, "End;\n",5); return 0; Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
*.SRF. ® 13.8.1. Installing the HERACELL 150i/240i GP 1. Starting the installation routine: − On the data CD in the subdirectory , double-click on the file to open PROGRAMS SETUP.EXE this file. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
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150i/240i GP User menu structure: The user surface is divided into two main menus: • MAIN with two functional elements: − Program version output FIRMWARE VERSION − Switch for exiting the program: QUIT Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
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− PASSWORD to prevent access to the device parameters of the incubator. Function of the user menu: PRESETTING The submenu PRESETTING is used to set the transmission speed and to select the serial port. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
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1. Example of a query for the currently measurable temperature values of the incubator: − Query: ? (default, not changeable) − Address: 2010 (temperature value address: Set value, actual value, reference value) 2. Send the query to the incubator: Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
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The submenu DATE & TIME is used for adjusting date and time to the desired time zone. 1. Data in the two text boxes must be entered in the format DD.MM.YY (day, month, year). 2. Accept the input data: − Press the button. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
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• Saving data sets as files: − Press the button. SAVE TO FILE DATA LOGGER The submenu DATA LOGGER is used for reading the event entries into the text box of the user surface. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
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Service of Thermo Fisher Scientific for fault analysis. 1. Create the service file: − Press the button. CREATE SERVICEFILE − Define file name and saving directory in the Windows dialog box. 2. Start saving process: − Press button. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
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The compilation of device informations and the creation of the service file may take some time. PASSWORD The submenu PASSWORD can only be accessed by the service personnel of Thermo Fisher Scientific. Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
Chapter 14 Device Log 14. Device Log Please list carried out works here: Device type: Part number: Serial number: Service number: Location: Operator's note: Work carried out Notes Date Signature Heracell 150i / 240i GP 50159351_B_06-2020 Thermo Scientific...
Further information on our compliance with these Directives, the recyclers in your country, and information on Thermo Scientific products which may assist the detection of substances subject to the RoHS Directive are available at www.thermofisher.com/WEEERoHS.
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