1. Manuals
  2. Brands
  3. Bioneer Manuals
  4. Laboratory Equipment
  5. ExiPrep 16 Dx
  6. User manual

Bioneer ExiPrep 16 Dx User Manual

Fully automated nucleic acid extraction system
Hide thumbs Also See for ExiPrep 16 Dx:
Table of Contents

Advertisement

Quick Links

Download this manual
User Manual(Ver 1.0)
ExiPrep™16 Dx
Fully Automated Nucleic Acid Extraction System

Advertisement

Table of Contents
loading

Related Manuals for Bioneer ExiPrep 16 Dx

Summary of Contents for Bioneer ExiPrep 16 Dx

  • Page 1 User Manual(Ver 1.0) ExiPrep™16 Dx Fully Automated Nucleic Acid Extraction System...

  • Page 2: Table Of Contents

    Configuring the System ..............................32 Administrator Management ............................33 16 Dx ..................35 VIII. Updating the ExiPrep IX. DNA/ RNA Extraction Program Number List ..............38 Troubleshooting ......................42 XI. Warranty ........................46 Copyright © 2011 by Bioneer Corporation. All rights reserved.

  • Page 3: Getting Started

    Bioneer Corporation. ▪ Bioneer Corporation reserves the right to alter, modify and otherwise make changes to the instrument and manuals without prior notice. ▪ You must be used carefully UV Lamp. Detail can be found in Safety warning and Precautions.

  • Page 4: Safety Warnings And Precautions

    II. Safety Warnings and Precautions The warnings and precautions stated below are for the correct and safe operation of the instrument. Please heed all information for your safety. Bioneer Corporation does not assume responsibility for non-compliance with the safety warnings and precautions stated below.

  • Page 5: Precautions And Warnings Regarding Instrument Installation

    ExiPrep 16 Dx Fully Automated Nucleic Acid Extraction System completely dry. Wet or moist hands may cause electric shock. Avoid placing objects in the front and rear of the instrument. Avoid installing the instrument in a dusty environment. Excessive dust may cause malfunction or damage to the instrument.

  • Page 6: Precautions And Warnings Regarding Product Usage And Maintenance

    ExiPrep 16 Dx Fully Automated Nucleic Acid Extraction System the mesh is damaged and the Cooling Fan does not work, the Cooling Fan motor and cooling element may overheat and cause fire. Set your PC's Power Options for 'Turn off the display', 'Turn off the hard disk', 'Standby mode' and 'Sleep mode' to 'Never'.

  • Page 7: Uv Lamp

    ExiPrep 16 Dx Fully Automated Nucleic Acid Extraction System 13) The instrument automatically turns off the UV lamp and its operation when the instrument’s door is open. However, just in case it does NOT turns off when the door is open, please make sure the UV light does not directly expose to your eyes and skin.

  • Page 8: System Components And Specifications

    ExiPrep 16 Dx Fully Automated Nucleic Acid Extraction System III. System Components and Specifications 1. System Components Part Name Cat. No. Qty. Check ExiPrep™16 Dx A-5050 1 ea □ User’s Manual 1 ea □ Waste Tray 1 ea □ Sample Tube Rack 1 ea □...

  • Page 9: Specifications

    ExiPrep 16 Dx Fully Automated Nucleic Acid Extraction System 2. Specifications Dimensions (mm) 320 (W) x 500 (H) x 535 (D) Weight 27 kg Operating temperature 15 – 30°C Operating humidity 20 - 80%, no condensation Operating system Standalone or PC Electrical (Voltage / Frequency) 100- 240 VAC, 50/60 Hz Network support...

  • Page 10: Installing The Exiprep™16 Dx

    ExiPrep 16 Dx Fully Automated Nucleic Acid Extraction System IV. Installing the ExiPrep™16 Dx Clear and clean the area where the instrument will be installed. Open the instrument door and remove the holding accessories. Make sure that all components are included. Refer to the list of components on page 1.
  • Page 11 ExiPrep 16 Dx Fully Automated Nucleic Acid Extraction System IV. Installing the ExiPrep™16 Dx (continued) Connect the power cable to the rear of the instrument. 6. Turn on the instrument. A power button will display on the LCD touch screen to indicate normal power on. 7.
  • Page 12 ExiPrep 16 Dx Fully Automated Nucleic Acid Extraction System IV. Installing the ExiPrep™16 Dx (continued) 10. Connect the instrument to the network hub using a LAN cable. 11. Connect a LAN cable between the network hub and the PC which has the ExiStation™ Manager Software installed. 12.

  • Page 13: Dna/ Rna Extraction

    ExiPrep 16 Dx Fully Automated Nucleic Acid Extraction System V. DNA/ RNA Extraction 1. Contamination Protection Accessory Installation From the ‘Menu’ screen, click ‘MISC SET’. Pull out the Syringe block all the way front (outside) Contamination Shield and Tip Protector. NOTE “Load”...

  • Page 14: Sample Preparation

    ExiPrep 16 Dx Fully Automated Nucleic Acid Extraction System 2. Sample Preparation 1. Place ‘Setup tray (A)’ on a flat surfaced desk. 2. On the Setup tray, put on the ‘Buffer Cartridge ① ② (B)’, ‘Disposable tip rack (C)’. ‘Setup Tray’ and ‘Tip Rack’ are supplied with ExiPrep 16 Dx.
  • Page 15 ExiPrep 16 Dx Fully Automated Nucleic Acid Extraction System 2. Sample Preparation (Continued) 7. Punch holes in the sealing film of Buffer Cartridge ① corresponding to the relative location and number of tubes and tips, using the punch tool. 8. Punch holes in sealing the film of Buffer Cartridge ② using the punch tool in the same pattern as Buffer Cartridge ①.

  • Page 16: Run (Not Used Pc)

    ExiPrep 16 Dx Fully Automated Nucleic Acid Extraction System 3. RUN (Not used PC) Connect the power cable to the rear of the instrument and separate the LAN cable of the instrument. Press the ‘Store Off’ button to turn the Cooling Block and cooling fan on.
  • Page 17 ExiPrep 16 Dx Fully Automated Nucleic Acid Extraction System RUN ( Not used PC) (Continued) Select the ‘elution volume’ from the LCD touchscreen. After selecting the desired ‘elution volume’ press ‘ok’ to complete PREP SETUP. Open the instrument door and pull out the Base Plate. 10.
  • Page 18 ExiPrep 16 Dx Fully Automated Nucleic Acid Extraction System 3. RUN( Not used PC) (Continued) 13. After the extraction run is complete, pull out the Base Plate and remove the Elution tubes, Buffer Cartridges and all racks from the Base Plate. After removing all accessories, push the Base Plate back in completely and close the door.
  • Page 19 ExiPrep 16 Dx Fully Automated Nucleic Acid Extraction System ※ Setup process according to the CHECK LIST Place the Buffer Cartridge ② on the heating block of the base plate. If Buffer Cartridge ② is not properly placed on the heating block, it may cause an experiment failure or an instrument malfunction.

  • Page 20: Existation™ Manager Software

    ExiPrep 16 Dx Fully Automated Nucleic Acid Extraction System VI. ExiStation™ Manager Software Press the instrument power button located on the upper-right side to turn the instrument on. Initialize the instrument by pressing the power icon initially displayed on the LCD touch screen. Execute the ExiStation™...
  • Page 21 ExiPrep 16 Dx Fully Automated Nucleic Acid Extraction System VI. ExiStation™ Manager Software (continued) Click the pull-down arrow for ‘Diagnosis Kit.’ A popup will appear. Select the diagnostic kit to be used for the experiment from the pull-down menus. Select and verify the ‘Sample Source Type’ selection. A popup prompting user input on the usage of the Buffer Cartridges will appear.
  • Page 22 ExiPrep 16 Dx Fully Automated Nucleic Acid Extraction System VI. ExiStation™ Manager Software (continued) The program will automatically allocate the NTC and PC wells. The default setting is one (1) each of NTC and PC. The number of NTC and PC wells can be changed through the ExiStation™ Manager Software.
  • Page 23 ExiPrep 16 Dx Fully Automated Nucleic Acid Extraction System VI. ExiStation™ Manager Software (continued) 10) Click the 'Sample ID' box and use a (optional) barcode reader or keyboard to enter sample information. 11) If you need to change sample ID information, click on the well number to summon a popup window. You may rename or delete the sample information and move the well to a different location.
  • Page 24 ExiPrep 16 Dx Fully Automated Nucleic Acid Extraction System VI. ExiStation™ Manager Software (continued) 13) Click the ‘RUN(▶)' button to initiate nucleic acid extraction. 14) After the extraction is complete, remove the samples (Diagnostic Kit tubes containing eluted material) and immediately run real-time PCR. 15) Pull out the Base Plate and remove all accessories including Elution tubes, Buffer Cartridges and Tips.
  • Page 25 ExiPrep 16 Dx Fully Automated Nucleic Acid Extraction System VI. ExiStation™ Manager Software (continued) 17) The UV sterilization process will take 5 minutes. Progress can be verified through the progress bar displayed on the instrument LCD touchscreen panel. 18) After click on the “Load” button, removes the tip protector and the contamination shield. A4(210X297) -23-...

  • Page 26: Exiprep Tm 16 Dx Setup

    Once the initialization has completed successfully, the LCD touchscreen will display the MENU as shown below. Please contact Bioneer Customer Service or your local sales representative if the initialization progress bar does not change for over 5 minutes during initialization or if the MENU screen does not appear after initialization.
  • Page 27 ExiPrep 16 Dx Fully Automated Nucleic Acid Extraction System VII. ExiPrep 16 Dx Setup (Continued) 1. Main Menu (continued) 1) PREP SETUP MENU Screen PREP SETUP Screen Selecting ‘Start’ from the Main Menu will bring up the ‘PREP SETUP’ screen where you can enter the three-digit code for the extraction and sample source type.
  • Page 28 ExiPrep 16 Dx Fully Automated Nucleic Acid Extraction System VII. ExiPrep 16 Dx Setup (Continued) 1. Main Menu (continued) 2) UV sterilization (UV lamp) MENU Screen UV Sterilization Screen Use the built-in UV-lamp to sterilize the internal cavity of the instrument. Press the ‘UV lamp’...
  • Page 29 ExiPrep 16 Dx Fully Automated Nucleic Acid Extraction System VII. ExiPrep 16 Dx Setup (Continued) 1. Main Menu (continued) 3) System setup menu (SETUP) Main Menu Screen Setup Screen Icon Description User registration menu You may create new accounts through this menu. Details on account creation can be found in page System configuration menu...

  • Page 30: Registering A New User

    ExiPrep 16 Dx Fully Automated Nucleic Acid Extraction System VII. ExiPrep 16 Dx Setup (Continued) 2. Registering a New User 16 Dx provides a user login option restricting the use of the instrument to registered users ExiPrep only. Enabling the user login option will limit non-user access to the instrument. Do not forget your user ID if you have enabled the user login option Press the ‘Setup’...

  • Page 31: Viewing Run History

    ExiPrep 16 Dx Fully Automated Nucleic Acid Extraction System VII. ExiPrep 16 Dx Setup (continued) 3. Viewing Run History If the login option is enabled, the user ID, process type and run status of each run is saved. Up to 99 most recent runs are saved in memory.

  • Page 32: Managing The Login Mode

    ExiPrep 16 Dx Fully Automated Nucleic Acid Extraction System VII. ExiPrep 16 Dx Setup (continued) 4. Managing the Login Mode The instrument provides a login mode for restricting non-registered use. Without a user ID, you would have limited access to instrument functions. Do not forget your user ID. Press the ‘Setup’...

  • Page 33: Managing The Automatic Uv-Sterilization Mode

    ExiPrep 16 Dx Fully Automated Nucleic Acid Extraction System VII. ExiPrep 16 Dx Setup (continued) 5. Managing the Automatic UV-Sterilization Mode The instrument provides an automatic UV-Sterilization mode to sterilize the instrument after every DNA/ RNA extraction run. Press the ‘Setup’ button to access the SETUP menu. Press the ‘Config’...

  • Page 34: Configuring The System

    ExiPrep 16 Dx Fully Automated Nucleic Acid Extraction System VII. ExiPrep 16 Dx Setup (continued) 6. Configuring the System Only the single user with a registered administrator ID is able to configure the system. Do not forget the administrator ID. Press the ‘Config’...

  • Page 35: Administrator Management

    ExiPrep 16 Dx Fully Automated Nucleic Acid Extraction System VII. ExiPrep 16 Dx Setup (continued) 7. Administrator Management Press the ‘Admin’ button from the System Config menu to access the Admin Access menu. Enter the 6-digit administrator ID using the keypad on the LCD touchscreen.
  • Page 36 ExiPrep 16 Dx Fully Automated Nucleic Acid Extraction System VII. ExiPrep 16 Dx Setup (continued) 7. Administrator (continued) Administrator ID change menu Enter a new 6-digit administrator ID using the keypad in the middle of LCD touchscreen and press ‘Enter’ button to save.

  • Page 37: Updating The Exiprep

    Updating software may improve instrument functionality and install up-to-date protocols for nucleic acid extraction. Please refer to the FAQ in our homepage or contact Bioneer Service Center if updating does not progress as described below or you have questions. NOTE: This program NOT included with the instrument. If you want to the program, you have to request to us.
  • Page 38 ExiPrep 16 Dx Fully Automated Nucleic Acid Extraction System VIII. Updating the ExiPrep 16 Dx (Continued) If the connection fails, the ‘connect fail’ prompt will be displayed. In this case, reboot the instrument and network hub, and verify that the network cable is connected correctly.
  • Page 39 ExiPrep 16 Dx Fully Automated Nucleic Acid Extraction System the PC fails to connect to the instrument, try the following steps. ※ Select ‘Local Area Connection’ from the control panel. Right-click the connection and select ‘Properties’. Select ‘Internet Protocol (TCP/IP)’. Select ‘Use the following IP address’.

  • Page 40: Dna/ Rna Extraction Program Number List

    ExiPrep 16 Dx Fully Automated Nucleic Acid Extraction System IX. DNA/ RNA Extraction Program Number List Target Sample source Target Sample source Genomic DNA Whole blood Total RNA Whole blood 1 01 2 01 Genomic DNA Animal tissue Total RNA Animal tissue 1 02 2 02...
  • Page 41 ExiPrep 16 Dx Fully Automated Nucleic Acid Extraction System Target Sample source Target Sample source mRNA Whole blood viral DNA Whole blood 3 01 4 01 mRNA Animal tissue viral DNA Animal tissue 3 02 4 02 mRNA FFPE tissue viral DNA FFPE tissue 3 03...
  • Page 42 ExiPrep 16 Dx Fully Automated Nucleic Acid Extraction System Target Sample source Target Sample source viral RNA Whole blood viral DNA/ RNA Whole blood 5 01 6 01 viral RNA Animal tissue viral DNA/ RNA Animal tissue 5 02 6 02 viral RNA FFPE tissue viral DNA/ RNA...
  • Page 43 ExiPrep 16 Dx Fully Automated Nucleic Acid Extraction System Target Sample source Target Sample source Plasmid DNA endA(+) strain Protein His-Tag 7 01 9 01 Plasmid DNA endA(-) strain 7 02 Target Sample source Fragment DNA Gel slice 8 21 Fragment DNA PCR product 8 22...

  • Page 44: Troubleshooting

    ExiPrep 16 Dx Fully Automated Nucleic Acid Extraction System X. Troubleshooting Error Solution The Power is not on. 1. Make sure the power connector is connected. 2. Check if the system is connected to the adaptor. 3. Check if the system power is pushed. 4.
  • Page 45 ExiPrep 16 Dx Fully Automated Nucleic Acid Extraction System Error Solution The base plate does not slide in Check if there are any obstacles or residues that may interrupt completely. moving the base plate. Check if all the accessories are inserted correctly. Request for After Service.
  • Page 46 ExiPrep 16 Dx Fully Automated Nucleic Acid Extraction System Error Solution It stops while operating. Check if the power supplied into the system. Check if the Power switch has been pressed. In the lower part of LCD screen, check if there is a delay in the blue progress bar.
  • Page 47 ExiPrep 16 Dx Fully Automated Nucleic Acid Extraction System Error Solution The Sample Block does not 1. Inspect the power supply. maintain a cold temperature. 2. Verify the Sample Block status through the LCD screen or PC software. 3. Request service from your dealer. The cooling fan does not function.

  • Page 48: Warranty

    This instrument is warranted by Bioneer against manufacturing defects in materials and workmanship for a limited warranty period of one (1) year from the date you received your product. Bioneer will either (1) repair the product at no charge if a hardware defect is found or (2) exchange the product if the same hardware defect arises more than three times during the limited warranty period.

Table of Contents