Related Manuals for Bioneer Exicycler 384
Summary of Contents for Bioneer Exicycler 384
- Page 1 Exicycler™ 384 Real-Time Quantitative Thermal Block 35℃ 15℃ A-2061 MT Promedt Consulting GmbH Altenhofstr. 80 D-66386 St. Ingbert. Germany. Tel +49 6894-58 10 20...
- Page 2 Exicycler™ 384 Exicycler™ 384 Real-Time Quantitative Thermal Block User’s Guide Bioneer Corporation 8-11, Munpyeongseo-ro, Daedeok-gu, Daejeon 34302, Republic of Korea Tel: +82-42-930-8777 Fax: +82-42-930-8688 Email: sales@bioneer.co.kr www.bioneer.com...
- Page 3 It is the responsibility of the purchaser to determine the suitability of the product for its particular use. Liability is conditional upon the customer providing full details of the problem to Bioneer within 30 days. Trademark Exicycler ™...
- Page 4 Exicycler™ 384...
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Page 5: Table Of Contents
™ 384 Software ............32 Performance Maintenance ......................47 Performing System Diagnosis using ExiCfg ............... 48 ® Guide to AccuPower Fluorescence test kit for calibration ............50 Calibration using ExiCfg ....................52 Cleaning the main body ....................62 BQ-042-101-12 Revision : 0 (2018-07-05 www.bioneer.com... - Page 6 Exicycler™ 384 Replacing Fuses ......................63 Exicycler Returning ™ 384 for Service ................64 Troubleshooting........................65 Ordering Information and Warranty .....................70 BQ-042-101-12 Revision : 0 (2018-07-05 www.bioneer.com...
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Page 7: Before Starting
Exicycler™ 384 Before Starting BQ-042-101-12 Revision : 0 (2018-07-05 www.bioneer.com... -
Page 8: How To Use This Guide
Exicycler ™ system is designed and sold for in vitro diagnostic (IVD) applications in combination with the diagnostic kits manufactured by Bioneer and labeled for diagnostic purposes. Exicycler ™ system can also be used for life science research and other laboratory purposes. - Page 9 Stay clear of this area when the door is in operation. Unawareness can cause injury. Francais Risque d’écrasement Ne pas rester proche de la porte durant son fonctionnement. Risque d’écrasement de doigts en cas de contact avec la porte. BQ-042-101-12 Revision : 0 (2018-07-05 www.bioneer.com...
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Page 10: Symbols On Exicycler ™ 384
Follow local municipal waste ordinances for proper disposal provisions to reduce environmental impact of waste from electrical and electronic instrument. European Union customers: Call your local European office for Bioneer instruments pick-up and recycling. BQ-042-101-12 Revision : 0 (2018-07-05 www.bioneer.com... -
Page 11: Safety Instructions
Exicycler™ 384 Safety Instructions BQ-042-101-12 Revision : 0 (2018-07-05 www.bioneer.com... -
Page 12: Safety Warnings And Cautions
Wet or moist hands may cause electric shock. A convenient and safe power cord should be available. The power cord provided by our company should be used. Socket outlets should be at least 1.5 m from a sink or wash basin. BQ-042-101-12 Revision : 0 (2018-07-05 www.bioneer.com... - Page 13 ™ 384 may get damaged when the instrument falls or is exposed to excessive physical shock. 6) Unplug the power cable from Exicycler ™ 384 when not in operation for a long period of time to prevent the possibility of fire by overheating. BQ-042-101-12 Revision : 0 (2018-07-05 www.bioneer.com...
- Page 14 ™ 384 should be operated in a clean condition. Contaminants such as dust can cause problems and reduce the life span of Exicycler ™ 384. Please prevent dust from entering into Exicycler ™ Exicycler 384. In order to extend life span of ™ 384, thoroughly remove dust periodically. BQ-042-101-12 Revision : 0 (2018-07-05 www.bioneer.com...
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Page 15: Introduction
Exicycler™ 384 Introduction BQ-042-101-12 Revision : 0 (2018-07-05 www.bioneer.com... -
Page 16: Overview
Five filter sets are provided for various applications. Therefore, no additional band path filters is required. Also, Bioneer’s imaging technique based on polarization of light enhances detection sensitivity for robust and reliable results. Patented (KR10-1089045, US8427643) polarizing optical apparatus mitigates the common problem of a reflection light, allowing precise quantification and target discrimination. -
Page 17: System Components And Specifications
Exicycler™ 384 System Components and Specifications BQ-042-101-12 Revision : 0 (2018-07-05 www.bioneer.com... -
Page 18: Overview
Overview Exicycler ™ 384 Real-time Quantitative Thermal Block is warranted by Bioneer against manufacturing defects in materials and workmanship for a limited warranty period of one year. Bioneer will charge for repairing products for the following conditions: Fault caused by the customer. - Page 19 Position Excitation (㎚) Emission (㎚) Fluorescent dye Blank Blank FAM, SYBR Green I JOE, TET TAMRA, CY3 Texas Red, ROX Chemical hazard: Exposure to following florescent dyes may cause eye and skin and respiratory tract BQ-042-101-12 Revision : 0 (2018-07-05 www.bioneer.com...
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Page 20: System Views
Exicycler™ 384 irritation. Read the MSDS before use and follow the instructions if swallowed or inhaled. Wear appropriate protective eyewear, clothing, and gloves. System Views BQ-042-101-12 Revision : 0 (2018-07-05 www.bioneer.com... -
Page 21: Installation And System Operation
Exicycler™ 384 Installation and System Operation BQ-042-101-12 Revision : 0 (2018-07-05 www.bioneer.com... -
Page 22: Overview
Scissors, pocket knife, or box cutter Exicycler Unpacking ™ 384 Save the packing materials and box in case you need to ship the instrument to Bioneer for service. Exicycler 1) To unpack ™ 384: Cut the straps securing the instrument box. -
Page 23: Setting Up The Computer
™ 384 Real-Time Quantitative Thermal Block operates through a computer. Therefore, to install Exicycler ™ 384, a computer or a laptop is necessary. Computer must meet the following requirements listed below. Please contact Bioneer Customer Service Center if you have any questions regarding installation. Computer Requirements –... -
Page 24: Installing The Operation And Analysis Program
Remaining program folders may cause errors during installation. This section is for the first time installation or re-installation of the software. The Bioneer Package has to be installed prior to installing Exicycler384 software. The procedures of Bioneer Package installation is described below. - Page 25 Back. Click Install to start installation. 6) This process takes several minutes. Please do not cancel until installation is complete. 7) When installation is complete, please click Finish to finish installation Shield Wizard. BQ-042-101-12 Revision : 0 (2018-07-05 www.bioneer.com...
- Page 26 10) Click OK for installing R(D)COM interface. 11) R icon will be shown on the Desktop when installation is successful. 12) The folders shown here are created after installation has finished. Please confirm ‘Bioneer_Package’ and sub folders. BQ-042-101-12 Revision : 0 (2018-07-05 www.bioneer.com...
- Page 27 3) A setup window asking about the license agreement is displayed. 4) Select I agree to activate install button, Click Install to begin installation. 5) All components related to .Net Framework are being installed. BQ-042-101-12 Revision : 0 (2018-07-05 www.bioneer.com...
- Page 28 Exicycler™ 384 6) Installation of .NET Framework 1.1 is complete. Click OK to finish installation. BQ-042-101-12 Revision : 0 (2018-07-05 www.bioneer.com...
- Page 29 2) Click Next and go to the next step. 3) Click Install to begin installation. If you want to change any settings or cancel installing, click Back or Cancel. 4) Please wait while Install-Shield Wizard installs Exicycler384. BQ-042-101-12 Revision : 0 (2018-07-05 www.bioneer.com...
- Page 30 Copy ‘Calibration’ folder from ™ 384 CD into C:\Exicycler384\Run folder. Exicycler CD 8) Run ‘NcBulkDriverSetup.exe’ to install the camera driver of Exicycler ™ 384. 9) When you run the file, the driver will be installed automatically. BQ-042-101-12 Revision : 0 (2018-07-05 www.bioneer.com...
- Page 31 Exicycler 14) Click Run Exicycler384 icon to start ™ 384 software. 15) You can see this screen when installation is completed successfully. The software version is also indicated. BQ-042-101-12 Revision : 0 (2018-07-05 www.bioneer.com...
- Page 32 Exicycler™ 384 Exicycler 16) Now you are ready to operate ™ 384 system. BQ-042-101-12 Revision : 0 (2018-07-05 www.bioneer.com...
- Page 33 If the serial Exicycler number matches with the connected ™ 384 system, click Copy button on the right hand side of window. 6) Please wait for a few minutes to complete. BQ-042-101-12 Revision : 0 (2018-07-05 www.bioneer.com...
- Page 34 3. An Error message is displayed when lunching ‘Run Exicycler384’ software. This message indicates that ‘.NET Framework 1.1’ has not yet been installed. Go to ‘Installing.NET Framework 1.1 Package’ section and re-install as described. BQ-042-101-12 Revision : 0 (2018-07-05 www.bioneer.com...
- Page 35 Exicycler™ 384 4. The Bioneer Package is not installed correctly. If an incorrect installation was performed previously, ‘Bioneer Package’ may not be installed correctly. Go to Control Panel-Programs Add/Remove and remove 4 programs from the computer - ‘Exicycler 384 V1 1.0’, ‘Bioneer package’, ‘R for windows 2.11.1’ and ‘R/Scilab(D)COM Server3.01-B5’. After removing them from the computer, repeat the entire installation process.
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Page 36: Turning On And Self-Diagnosis
Do not place any obstacle in front of the system and do not load a plate or take it out when the door is moving. It can cause serious damage and lead to severe injury. BQ-042-101-12 Revision : 0 (2018-07-05 www.bioneer.com... -
Page 37: Running Real-Time Pcr Using Exicycler ™ 384 Software
Protocol Information displays a cycling protocol in detail. d. Well information displays sample and probe specification for each well. e. Temperature Profile displays a temperature curve of the cycling protocol. BQ-042-101-12 Revision : 0 (2018-07-05 www.bioneer.com... - Page 38 In the Dye drop-down list of the Add Probe window, select SYBR_Green. Select None in the Quencher drop-down list. Select your desirable color for display and then click OK. 6) Check if your new probe is shown under the list, and then click Save. BQ-042-101-12 Revision : 0 (2018-07-05 www.bioneer.com...
- Page 39 Protocol Information: Displays a cycling protocol in detail. d. Temperature Profile: Displays a temperate curve of the cycling protocol. e. Protocol Setting: Specifies protocol specifications such as temperature, time, and a number of cycles. BQ-042-101-12 Revision : 0 (2018-07-05 www.bioneer.com...
- Page 40 Scan is used to detect fluorescence signals from samples. The fluorescence signals are measured for about 25 seconds at set temperature of the previous incubation step. If the Scan step is not included in the protocol file, a conventional PCR will be carried out without scanning fluorescence signals. BQ-042-101-12 Revision : 0 (2018-07-05 www.bioneer.com...
- Page 41 12) Repeat steps 10 and 11 if needed (i.e. 94°C 30 sec / 55°C 30 sec / 72°C 30 sec). NOTE: To edit Incubate steps, click one of the steps in the Protocol Information window and edit. Click Update to change the protocol. BQ-042-101-12 Revision : 0 (2018-07-05 www.bioneer.com...
- Page 42 To set the temperature on the Hot top Make sure that the Hottop check box is selected. The default value is set to 105 ℃. If you turn off the Check box hot top is not heated. BQ-042-101-12 Revision : 0 (2018-07-05 www.bioneer.com...
- Page 43 It will show predicted temperatures on each well. ③ Click Append. 18) Enter a protocol file name in the Protocol field at the top left (i.e. “Test”) and then click button to save the protocol. BQ-042-101-12 Revision : 0 (2018-07-05 www.bioneer.com...
- Page 44 Add Probe adds the probe to be used in experiment. NOTE: Ensure that you specify appropriate information (i.e. probe set up and sample type) for each well before running experiment in order to generate accurate data. BQ-042-101-12 Revision : 0 (2018-07-05 www.bioneer.com...
- Page 45 Fluorescent Dye: displays name of a fluorescence dye assigned by the user. c. Quencher: displays name of a quencher assigned by the user. 22) The probe is loaded and shown as follows: 23) Select wells from the 384-well plate diagram. BQ-042-101-12 Revision : 0 (2018-07-05 www.bioneer.com...
- Page 46 IPC- is a sample that does not contain a template of IPC. 25) For example, Click well D3 and enter ‘1000000’ for a concentration of 10 -copy and then click Assign. Repeat this for wells D4 through D8 with concentrations of serial diluted standards. BQ-042-101-12 Revision : 0 (2018-07-05 www.bioneer.com...
- Page 47 30) The protocol file and plate file are saved. Enter a master file name in the Master field (i.e. Test) and then click button to save the master file. BQ-042-101-12 Revision : 0 (2018-07-05 www.bioneer.com...
- Page 48 Load the 384-well plate with A1 positioned at top-left corner of the block. 34) Verify the master file and go to Run > Run from the top menu bar or click the Run button. BQ-042-101-12 Revision : 0 (2018-07-05 www.bioneer.com...
- Page 49 This message will appear during lamp ignition. This message will appear when lamp is turned on and Exicycler initialization of ™ 384. 37) The main window will appear when the progress bar finishes without any problems. BQ-042-101-12 Revision : 0 (2018-07-05 www.bioneer.com...
- Page 50 *.ex8 file is created under a folder designated by the user after the experiment is complete. The user Exicycler can analyze the *.ex8 file using ™ 384 Analysis Program to generate analysis data. Please refer to Analyzing Data using Analysis Program for data analysis. BQ-042-101-12 Revision : 0 (2018-07-05 www.bioneer.com...
- Page 51 ™ Greenstar™ qPCR Mater Mix may cause eye and skin irritation, and respiratory tract irritation. Read MSDS before use and follow the instructions if swallowed or inhaled. Wear appropriate protective eyewear, clothing, and gloves. BQ-042-101-12 Revision : 0 (2018-07-05 www.bioneer.com...
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Page 52: Performance Maintenance
Exicycler™ 384 Performance Maintenance BQ-042-101-12 Revision : 0 (2018-07-05 www.bioneer.com... -
Page 53: Performing System Diagnosis Using Exicfg
‘USB Communication is NOT initialized’ message box will pop up if ™ 384 is turned off or if the Exicycler cable connection between ™ 384 and the computer is not firmly connected. 4) Click Start in the Self Test window. BQ-042-101-12 Revision : 0 (2018-07-05 www.bioneer.com... - Page 54 Any problems caused during self-diagonsis are recorded in ‘Error log’. 7) Check the Error Log screen at the bottom right of the program to see the problems. Click the ‘refresh’ button to display the error that occurred. BQ-042-101-12 Revision : 0 (2018-07-05 www.bioneer.com...
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Page 55: Guide To Accupower ® Fluorescence Test Kit For Calibration
2) Take out a sealed packs in the Fluorescence test kit. 3) Allow the pack to thaw in room temperature for at least 10 minutes. 4) Take out a Fluorescence test plate from its packaging. BQ-042-101-12 Revision : 0 (2018-07-05 www.bioneer.com... - Page 56 7) Put the Fluorescence test kit back into its packaging and return it to the freezer when the calibration is done. IMPORTANT: The Fluorescence test kit contains photosensitive components. Keep the Fluorescence test kit away from light during calibration. Put the Fluorescence test plate back into its packaging right away for reuse. BQ-042-101-12 Revision : 0 (2018-07-05 www.bioneer.com...
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Page 57: Calibration Using Exicfg
Block. You can either transfer proper calibration data associated with a serial number from the installation CD to the computer or perform the calibration by an authorized engineer of Bioneer. Install the Operation and Analysis Software for the calibration data transfer to the computer. For a reliable... - Page 58 You must perform the Mask Calbration test prior to any Fluorescence tests. IMPORTANT: It is important to load the Fluorescence test plate in the right position. A misplaced plate may cause damage or technical problems. BQ-042-101-12 Revision : 0 (2018-07-05 www.bioneer.com...
- Page 59 8) Click Start button in the ‘Mask Calibration’ window. NOTE: Exicycler The Calibration time is approximately 10 minutes depending on the condition of ™ 384 and the computer. 9) When the Mask Calibration is complete, click Close. BQ-042-101-12 Revision : 0 (2018-07-05 www.bioneer.com...
- Page 60 Wait at least 5 seconds before taking out the Fluorescence test plate from the 384-well block. The Fluorescence test plate is heated to a temperature of 100°C and may cause a burn. 10) The ‘Mask Calibration’ part will turn green(completed) when the Mask Calibration is completed. BQ-042-101-12 Revision : 0 (2018-07-05 www.bioneer.com...
- Page 61 It is important to load the Fluorescence test plate in the right position. A misplaced plate may cause damage or technical problems. 3) Click Background Calibration button. 4) Click Start in the ‘Background Calibration’ window. NOTE: The Background Calibration takes approximately 10 minutes depending on the condition of Exicycler ™ 384. BQ-042-101-12 Revision : 0 (2018-07-05 www.bioneer.com...
- Page 62 Wait at least 5 seconds before taking out the Fluorescence test plate from the 384-well block. The Fluorescence test plate is heated to a temperature of 100°C and may cause a burn. 6) The ‘Background Calibration’ part will turn green(completed) when the Background calibration is completed. BQ-042-101-12 Revision : 0 (2018-07-05 www.bioneer.com...
- Page 63 2) Click Multi-Channel Calibration button. 3) Select fluorescence dyes ‘Multi-Channel Calibration’ window for the Calibration. NOTE: 9 dyes are available for calibration. NOTE: For a selective multi-channel calibration of your desirable dyes, select check boxes of the dyes. BQ-042-101-12 Revision : 0 (2018-07-05 www.bioneer.com...
- Page 64 It is important to load the Fluorescence Test plate in the right position. A misplaced plate may cause damage or technical problems. 7) Click Yes in the ‘Confirm’ window. 8) The door will open when the Calibration is successfully complete. BQ-042-101-12 Revision : 0 (2018-07-05 www.bioneer.com...
- Page 65 “SYBR Green”) on the bath’ message appears. 10) Place the Multi-Channel Fluorescence Test plate on the block. 11) Click Continue and repeat step 9 through 10. 12) When the Multi-channel Calibration is complete, click Close in the ‘Multi-Channel Calibration’ window. BQ-042-101-12 Revision : 0 (2018-07-05 www.bioneer.com...
- Page 66 16) Turn off ™ 384 by pressing the POWER button. Exicycler 17) Turn off ™ 384 using the main power switch POWER button Exicycler located at the back of ™ 384. Main POWER switch BQ-042-101-12 Revision : 0 (2018-07-05 www.bioneer.com...
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Page 67: Cleaning The Main Body
2) If the hot-top cover become contaminated with amplified DNA, raise the hot-top cover to a cleaning position. Wipe the cover with a cloth swab soaked in isopropyl alcohol or DNA zap, then wipe the cover with a damp cloth. BQ-042-101-12 Revision : 0 (2018-07-05 www.bioneer.com... -
Page 68: Replacing Fuses
250V, F10AL, 5×20mm fuses. 5) Place the fuse compartment back into the power entry module and close the fuse door. 6) Press the fuse door until it locks in place. 7) Connect the instrument power cord. BQ-042-101-12 Revision : 0 (2018-07-05 www.bioneer.com... -
Page 69: Returning Exicycler ™ 384 For Service
Bioneer for service. 2) Complete certification for instrument decontamination. 3) Fax or e-mail(exicycler-support@bioneer.com) the service request form to the customer center. 4) Pack the instrument in the provided packaging, without any accessories or power cords. Include service request form in the box. -
Page 70: Troubleshooting
Exicycler™ 384 Troubleshooting BQ-042-101-12 Revision : 0 (2018-07-05 www.bioneer.com... - Page 71 Exicycler™ 384 Please take recommend action for each observation. Contact Bioneer Customer Service Center if problem continues after the recommended action. Problems Recommended Action Exicycler ™ does 1. Make sure the power cable is firmly connected to the wall circuit, start.
- Page 72 Check Box must be selected and correct temperature must be set up. 3. A small amount of evaporation is not critical. 4. It is recommended that you use Bioneer kits and reagents to prevent PCR sample evaporation. products 1. Perform gel electrophoresis to determine the presence of amplified.
- Page 73 Communication between machine and S/W was not initialized will Exicycler™ display if you click Run button when 384 is not ready. Do you want to stop PCR? will display if you click Stop button while PCR is still running. BQ-042-101-12 Revision : 0 (2018-07-05 www.bioneer.com...
- Page 74 20. UserName was already in User List will appear when entering an existing name for User set-up. 21. You must select Dye and Quencher will display if the dye and quencher set up has not been selected in the Add Probe window. BQ-042-101-12 Revision : 0 (2018-07-05 www.bioneer.com...
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Page 75: Ordering Information And Warranty
Exicycler™ 384 Ordering Information and Warranty BQ-042-101-12 Revision : 0 (2018-07-05 www.bioneer.com... - Page 76 Greenstar K-6253 qPCR Master Mix / 100Rxn, 50 μL reaction ® AccuPower Greenstar K-6254 qPCR Master Mix / 200Rxn, 50 μL reaction ® K-6603 AccuPower Plus DualStar ™ qPCR Master Mix(2X), 2.5ml, 100 rxn BQ-042-101-12 Revision : 0 (2018-07-05 www.bioneer.com...
- Page 77 This instrument is warranted by Bioneer against manufacturing defect in materials and workmanship for a limited warranty period of one (1) year from the date you received your product. Bioneer will either (1) repair the product at no charge if a hardware defect is found or (2) exchange the product if the same hardware defect arises more than three times during the limited warranty period.
- Page 78 8-11 Munpyeongseo-ro, Daedeok-gu, Daejeon, 34302, Republic of Korea +82-42-930-8777 (Korea: 1588-9788) +82-42-930-8688 sales@bioneer.com www.bioneer.com 155 Filbert St. Suite 216 Oakland, CA 94607, USA +1-877-264-4300 (Toll-free) +1-510-865-0350 order.usa@bioneer.com us.bioneer.com Korea Bio Park BLDG #B-702, 700 Daewangpangyo-ro, Bundang-gu, Seongnam-si Gyeonggi-do, 13488, Republic of Korea...