Bioneer ExiPrep 16 Pro User Manual
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Bioneer ExiPrep 16 Pro User Manual

Fully automated nucleic extraction system
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User Manual(Ver 1.1)
ExiPrep™16 Pro
Fully Automated Nucleic Acid Extraction System

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Summary of Contents for Bioneer ExiPrep 16 Pro

  • Page 1 User Manual(Ver 1.1) ExiPrep™16 Pro Fully Automated Nucleic Acid Extraction System...

  • Page 2: Table Of Contents

    Configuring the System ..............................31 Administrator Management ............................32 16 Pro ..................34 VIII. Updating the ExiPrep IX. DNA/ RNA Extraction Program Number List ..............37 Troubleshooting ......................41 XI. Warranty ........................45 Copyright © 2011 by Bioneer Corporation. All rights reserved.

  • Page 3: Getting Started

    Bioneer Corporation. ▪ Bioneer Corporation reserves the right to alter, modify and otherwise make changes to the instrument and manuals without prior notice. ▪ You must be used carefully UV Lamp. Detail can be found in Safety warning and Precautions.

  • Page 4: Safety Warnings And Precautions

    II. Safety Warnings and Precautions The warnings and precautions stated below are for the correct and safe operation of the instrument. Please heed all information for your safety. Bioneer Corporation does not assume responsibility for non-compliance with the safety warnings and precautions stated below.

  • Page 5: Precautions And Warnings Regarding Instrument Installation

    ExiPrep 16 Pro Fully Automated Nucleic Acid Extraction System completely dry. Wet or moist hands may cause electric shock. Avoid placing objects in the front and rear of the instrument. Avoid installing the instrument in a dusty environment. Excessive dust may cause malfunction or damage to the instrument.

  • Page 6: Precautions And Warnings Regarding Product Usage And Maintenance

    ExiPrep 16 Pro Fully Automated Nucleic Acid Extraction System the mesh is damaged and the Cooling Fan does not work, the Cooling Fan motor and cooling element may overheat and cause fire. Set your PC's Power Options for 'Turn off the display', 'Turn off the hard disk', 'Standby mode' and 'Sleep mode' to 'Never'.

  • Page 7: Uv Lamp

    ExiPrep 16 Pro Fully Automated Nucleic Acid Extraction System 13) The instrument automatically turns off the UV lamp and its operation when the instrument’s door is open. However, just in case it does NOT turns off when the door is open, please make sure the UV light does not directly expose to your eyes and skin.

  • Page 8: System Components And Specifications

    ExiPrep 16 Pro Fully Automated Nucleic Acid Extraction System III. System Components and Specifications 1. System Components Part Name Cat. No. Qty. Check ExiPrep™16 Pro A-5040 1 ea □ User’s Manual 1 ea □ Waste Tray 1 ea □ Elution Tube Rack 1 ea □...

  • Page 9: Specifications

    ExiPrep 16 Pro Fully Automated Nucleic Acid Extraction System 2. Specifications Dimensions (mm) 320 (W) x 500 (H) x 535 (D) Weight 27 kg Operating temperature 15 – 30°C Operating humidity 20 - 80%, no condensation Operating system Standalone or PC Electrical (Voltage / Frequency) 100- 240 VAC, 50/60 Hz Network support...

  • Page 10: Installing The Exiprep™16 Pro

    ExiPrep 16 Pro Fully Automated Nucleic Acid Extraction System IV. Installing the ExiPrep™16 Pro - Clear and clean the area where the instrument will be installed. - Open the instrument door and remove the belt tie and rail bolt. - Make sure that all components are included. Refer to the list of components on page 1.
  • Page 11 ExiPrep 16 Pro Fully Automated Nucleic Acid Extraction System IV. Installing the ExiPrep™16 Pro (continued) Connect the power cable to the rear of the instrument. 6. Turn on the instrument. A power button will display on the LCD touch screen to indicate normal power on. 7.
  • Page 12 ExiPrep 16 Pro Fully Automated Nucleic Acid Extraction System IV. Installing the ExiPrep™16 Pro (continued) 10. Connect the instrument to the network hub using a LAN cable. 11. Connect a LAN cable between the network hub and the PC which has the ExiStation™ Manager Software installed. 12.

  • Page 13: Dna/ Rna Extraction

    ExiPrep 16 Pro Fully Automated Nucleic Acid Extraction System V. DNA/ RNA Extraction 1. Contamination Shield Installation From the ‘Menu’ screen, click ‘MISC SET’. Pull out the Syringe block all the way front (outside) Contamination Shield. CAUTION! In order to use contamination shield, this must be installed on the Base plate accessory.

  • Page 14: Sample Preparation

    ExiPrep 16 Pro Fully Automated Nucleic Acid Extraction System V. DNA/ RNA Extraction (continued) 2. Sample Preparation 1. Place ‘Setup tray (A)’ on a flat surfaced desk. 2. On the Setup tray, put on the ‘Buffer Cartridge ① (D)’, ‘Elution tube rack (B), ‘Disposable tip rack (C)’. ‘Setup Tray’...
  • Page 15 ExiPrep 16 Pro Fully Automated Nucleic Acid Extraction System V. DNA/ RNA Extraction (continued) 2. Sample Preparation (Continued) 7. Punch holes in the sealing film of Buffer Cartridge ① corresponding to the relative location and number of tubes and tips, using the 6 Hole punch tool. 8.

  • Page 16: Run (Not Used Pc)

    ExiPrep 16 Pro Fully Automated Nucleic Acid Extraction System V. DNA/ RNA Extraction (continued) 3. RUN (Not used PC) Connect the power cable to the rear of the instrument and separate the LAN cable of the instrument. Press the ‘Store Off’ button to turn the Cooling Block and cooling fan on.
  • Page 17 ExiPrep 16 Pro Fully Automated Nucleic Acid Extraction System V. DNA/ RNA Extraction (continued) 3. RUN( Not used PC) (Continued) Select the ‘elution volume’ from the LCD touchscreen. After selecting the desired ‘elution volume’ press ‘ok’ to complete PREP SETUP. Open the instrument door and pull out the Base Plate.
  • Page 18 ExiPrep 16 Pro Fully Automated Nucleic Acid Extraction System V. DNA/ RNA Extraction (continued) 3. RUN( Not used PC) (Continued) 13. After the extraction run is complete, pull out the Base Plate and remove the Elution tubes, Buffer Cartridges and all racks from the Base Plate.
  • Page 19 ExiPrep 16 Pro Fully Automated Nucleic Acid Extraction System ※ Setup process according to the CHECK LIST 10a. Insert Buffer Cartridge ② on the Base plate. Make sure the Buffer Cartridge fits snugly and exactly into its place. Misplacement of the Buffer Cartridge may result in instrument break down and malfunction.

  • Page 20: Existation™ Manager Software

    ExiPrep 16 Pro Fully Automated Nucleic Acid Extraction System VI. ExiStation™ Manager Software Press the instrument power button located on the upper-right side to turn the instrument on. Initialize the instrument by pressing the power icon initially displayed on the LCD touchscreen. Execute the ExiStation™...
  • Page 21 ExiPrep 16 Pro Fully Automated Nucleic Acid Extraction System VI. ExiStation™ Manager Software (continued) Once the software module is loaded, select the diagnostic kit name to be used for the examination. Select the appropriate kit name by clicking on the small down arrow to summon a drop-down menu where you can select the desired kit by catalog number.
  • Page 22 ExiPrep 16 Pro Fully Automated Nucleic Acid Extraction System VI. ExiStation™ Manager Software (continued) If there were any Re-Prep orders on the queue, a popup will display all Re-Prep orders relevant to the Diagnostic Kit. Select the Re-Prep orders you wish to incorporate into the workflow at this point. The locations of the non-template control (NTC) and positive control (PC) will be automatically allocated.
  • Page 23 ExiPrep 16 Pro Fully Automated Nucleic Acid Extraction System VI. ExiStation™ Manager Software (continued) If you need to change sample ID information, click on the well number to summon a popup window. You may rename or delete the sample information and move the well to a different location. For example, to move the sample to a different well, select ‘Move’...
  • Page 24 ExiPrep 16 Pro Fully Automated Nucleic Acid Extraction System VI. ExiStation™ Manager Software (continued) 11) After well allocation is complete, press the ‘Store On’ icon (located at the bottom portion of the window) to turn the Cooling Block and cooling fan on. The Cooling Block maintains the Elution Tube Rack at a low temperature to keep the eluted nucleic acids and nucleic acid-diagnostic kit mix refrigerated.

  • Page 25: Exiprep Tm 16 Pro Setup

    Once the initialization has completed successfully, the LCD touchscreen will display the MENU as shown below. Please contact Bioneer Customer Service or your local sales representative if the initialization progress bar does not change for over 5 minutes during initialization or if the MENU screen does not appear after initialization.
  • Page 26 ExiPrep 16 Pro Fully Automated Nucleic Acid Extraction System VII. ExiPrep 16 Pro Setup (continued) 1. Main Menu (continued) 1) PREP SETUP MENU Screen PREP SETUP Screen Selecting ‘Start’ from the Main Menu will bring up the ‘PREP SETUP’ screen where you can enter the three-digit code for the extraction and sample source type.
  • Page 27 ExiPrep 16 Pro Fully Automated Nucleic Acid Extraction System VII. ExiPrep 16 Pro Setup (continued) 1. Main Menu (continued) 2) UV sterilization (UV lamp) MENU Screen UV Sterilization Screen Use the built-in UV-lamp to sterilize the internal cavity of the instrument. Press the ‘UV lamp’...
  • Page 28 ExiPrep 16 Pro Fully Automated Nucleic Acid Extraction System VII. ExiPrep 16 Pro Setup (continued) 1. Main Menu (continued) 3) System setup menu (SETUP) Main Menu Screen Setup Screen Icon Description User registration menu You may create new accounts through this menu. Details on account creation can be found in page System configuration menu...

  • Page 29: Registering A New User

    ExiPrep 16 Pro Fully Automated Nucleic Acid Extraction System VII. ExiPrep 16 Pro Setup (Continued) 2. Registering a New User 16 Pro provides a user login option restricting the use of the instrument to registered users ExiPrep only. Enabling the user login option will limit non-user access to the instrument. Do not forget your user ID if you have enabled the user login option Press the ‘Setup’...

  • Page 30: Viewing Run History

    ExiPrep 16 Pro Fully Automated Nucleic Acid Extraction System VII. ExiPrep 16 Pro Setup (continued) 3. Viewing Run History If the login option is enabled, the user ID, process type and run status of each run is saved. Up to 99 most recent runs are saved in memory.

  • Page 31: Managing The Login Mode

    ExiPrep 16 Pro Fully Automated Nucleic Acid Extraction System VII. ExiPrep 16 Pro Setup (continued) 4. Managing the Login Mode The instrument provides a login mode for restricting non-registered use. Without a user ID, you would have limited access to instrument functions. Do not forget your user ID. Press the ‘Setup’...

  • Page 32: Managing The Automatic Uv-Sterilization Mode

    ExiPrep 16 Pro Fully Automated Nucleic Acid Extraction System VII. ExiPrep 16 Pro Setup (continued) 5. Managing the Automatic UV-Sterilization Mode The instrument provides an automatic UV-Sterilization mode to sterilize the instrument after every DNA/ RNA extraction run. Press the ‘Setup’ button to access the SETUP menu. Press the ‘Config’...

  • Page 33: Configuring The System

    ExiPrep 16 Pro Fully Automated Nucleic Acid Extraction System VII. ExiPrep 16 Pro Setup (continued) 6. Configuring the System Only the single user with a registered administrator ID is able to configure the system. Do not forget the administrator ID. Press the ‘Config’...

  • Page 34: Administrator Management

    ExiPrep 16 Pro Fully Automated Nucleic Acid Extraction System VII. ExiPrep 16 Pro Setup (continued) 7. Administrator Management Press the ‘Admin’ button from the System Config menu to access the Admin Access menu. Enter the 6-digit administrator ID using the keypad on the LCD touchscreen.
  • Page 35 ExiPrep 16 Pro Fully Automated Nucleic Acid Extraction System VII. ExiPrep 16 Pro Setup (continued) 7. Administrator (continued) Administrator ID change menu Enter a new 6-digit administrator ID using the keypad in the middle of LCD touchscreen and press ‘Enter’ button to save.

  • Page 36: Updating The Exiprep

    Updating software may improve instrument functionality and install up-to-date protocols for nucleic acid extraction. Please refer to the FAQ in our homepage or contact Bioneer Service Center if updating does not progress as described below or you have questions. Connect ExiPrep™16 Pro to your computer using LAN cable.
  • Page 37 ExiPrep 16 Pro Fully Automated Nucleic Acid Extraction System VIII. Updating the ExiPrep 16 Pro (Continued) If the connection fails, the ‘connect fail’ prompt will be displayed. In this case, reboot the instrument and network hub, and verify that the network cable is connected correctly.
  • Page 38 ExiPrep 16 Pro Fully Automated Nucleic Acid Extraction System the PC fails to connect to the instrument, try the following steps. ※ Select ‘Local Area Connection’ from the control panel. Right-click the connection and select ‘Properties’. Select ‘Internet Protocol (TCP/IP)’. Select ‘Use the following IP address’.

  • Page 39: Dna/ Rna Extraction Program Number List

    ExiPrep 16 Pro Fully Automated Nucleic Acid Extraction System IX. DNA/ RNA Extraction Program Number List Target Sample source Target Sample source Genomic DNA Whole blood Total RNA Whole blood 1 01 2 01 Genomic DNA Animal tissue Total RNA Animal tissue 1 02 2 02...
  • Page 40 ExiPrep 16 Pro Fully Automated Nucleic Acid Extraction System Target Sample source Target Sample source mRNA Whole blood viral DNA Whole blood 3 01 4 01 mRNA Animal tissue viral DNA Animal tissue 3 02 4 02 mRNA FFPE tissue viral DNA FFPE tissue 3 03...
  • Page 41 ExiPrep 16 Pro Fully Automated Nucleic Acid Extraction System Target Sample source Target Sample source viral RNA Whole blood viral DNA/ RNA Whole blood 5 01 6 01 viral RNA Animal tissue viral DNA/ RNA Animal tissue 5 02 6 02 viral RNA FFPE tissue viral DNA/ RNA...
  • Page 42 ExiPrep 16 Pro Fully Automated Nucleic Acid Extraction System Target Sample source Target Sample source Plasmid DNA endA(+) strain Protein His-Tag 7 01 9 01 Plasmid DNA endA(-) strain 7 02 Target Sample source Fragment DNA Gel slice 8 21 Fragment DNA PCR product 8 22...

  • Page 43: Troubleshooting

    ExiPrep 16 Pro Fully Automated Nucleic Acid Extraction System X. Troubleshooting Error Solution The Power is not on. 1. Make sure the power connector is connected. 2. Check if the system is connected to the adaptor. 3. Check if the system power is pushed. 4.
  • Page 44 ExiPrep 16 Pro Fully Automated Nucleic Acid Extraction System Check if all the accessories are inserted correctly. Request for After Service. The system is not working even if Check if base plate is located in the correct. the door is closed. Check if the pair of magnet attached to the door is correctly attached.
  • Page 45 ExiPrep 16 Pro Fully Automated Nucleic Acid Extraction System Request for After Service. The System is running but there Check if wrong protocol has been used. is an error. Check if there is an error in the motor while operating during the movement of syringe block due to residues or accessories being stuck.
  • Page 46 ExiPrep 16 Pro Fully Automated Nucleic Acid Extraction System cable. 2. Restart the computer and instrument. 3. Update protocol 4. Re-update protocol software does 1. Inspect the network cables connecting the PC and network hub. operate the instrument. 2. Inspect the network cables connecting the instruments and network hub.

  • Page 47: Warranty

    This instrument is warranted by Bioneer against manufacturing defects in materials and workmanship for a limited warranty period of one (1) year from the date you received your product. Bioneer will either (1) repair the product at no charge if a hardware defect is found or (2) exchange the product if the same hardware defect arises more than three times during the limited warranty period.

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