1. Manuals
  2. Brands
  3. Bioneer Manuals
  4. Laboratory Equipment
  5. ExiPrep 16 Dx
  6. User manual

Bioneer ExiPrep 16 Dx User Manual

Fully automated nucleic acid extraction system
Hide thumbs Also See for ExiPrep 16 Dx:
Table of Contents

Advertisement

Quick Links

User's Guide
TM
ExiPrep
16 Dx
Fully Automated Nucleic Acid Extraction System
A-5050
Fully Automated Nucleic Acid Extraction System

Advertisement

Table of Contents
loading

Related Manuals for Bioneer ExiPrep 16 Dx

Summary of Contents for Bioneer ExiPrep 16 Dx

  • Page 1 User’s Guide ExiPrep 16 Dx Fully Automated Nucleic Acid Extraction System A-5050 Fully Automated Nucleic Acid Extraction System...
  • Page 2 Fully Automated Nucleic Acid Extraction System ExiPrep 16 Dx Fully Automated Nucleic Acid Extraction System User’s Guide Version No.: 1.01(2015-09) Bioneer 8-11, Munpyeongseo-ro, Daedeok-gu, Daejeon, 34302, Republic of Korea Tel: +82-42-930-8777 Fax: +82-42-930-8688 Email: sales@bioneer.com www.bioneer.com BQ-042-101-01 Revision : 0(2012-12-07) www.bioneer.com...
  • Page 3 ExiPrep 16 Dx / Fully Automated Nucleic Acid Extraction System BQ-042-101-01 Revision : 0(2012-12-07) www.bioneer.com...

  • Page 4: Table Of Contents

    Managing the Automatic UV-Sterilization Mode ....................30 Configuring the System ..............................31 Administrator Management ............................. 32 ExiPrep VIII. Updating the 16 Dx ................... 34 IX. DNA/RNA Extraction Program Number List ................ 37 Troubleshooting ......................41 XI. Warranty ........................45 BQ-042-101-01 Revision : 0(2012-12-07) www.bioneer.com...

  • Page 5: Getting Started

    Bioneer Corporation.  Bioneer Corporation reserves the right to alter, modify and otherwise make changes to the instrument and manuals without prior notice. ...
  • Page 6 ™16 Dx as unsorted municipal waste. Follow local municipal waste ordinances for proper disposal provisions to reduce the environmental impact of waste electrical and electronic Instrument. European Union customers: Call your local Europe office for Bioneer instruments pick-up and recycling. BQ-042-101-01 Revision : 0(2012-12-07) www.bioneer.com...
  • Page 7 40℃~90℃. Highly pure DNA or RNA can be purified with Bioneer’s proprietary silica magnetic bead technology. The magnetic beads feature extremely high binding capacity for DNA and RNA due to a wide surface area on the round-shape bead.

  • Page 8: Safety Warnings And Precautions

    II. Safety Warnings and Precautions The warnings and precautions stated below are for the correct and safe operation of the instrument. Please heed all information for your safety. Bioneer Corporation does not assume responsibility for non-compliance with the safety warnings and precautions stated below.
  • Page 9 1) Dust off the power plug and insert the plug so that the connection is firm and does not wiggle. Incomplete electrical contacts may cause fire. 2) Operate the instrument in an ambient temperature range of 15℃~30℃. Excessive exposure BQ-042-101-01 Revision : 0(2012-12-07) www.bioneer.com...
  • Page 10 Since installed Buffer Cartridges obscure the view, re-initialize the instrument or take out the Buffer Cartridges and visually inspect the position of the Heating Block before pulling out the Base Plate. BQ-042-101-01 Revision : 0(2012-12-07) www.bioneer.com...
  • Page 11 4) Read and understand the Material Safety Data Sheets(MSDS) provided by the chemical manufacturer before you store, handle, or work with any chemicals or hazardous materials. 5) Minimize contact with chemicals. Wear appropriate personal protective equipment when handling chemicals (for example, safety glasses, gloves, or protective clothing). BQ-042-101-01 Revision : 0(2012-12-07) www.bioneer.com...

  • Page 12: Waste Safety Warnings & User Maintenance

    Also, do not spray the cleaning solution directly onto the equipment. 3. Case of contamination Note When a contamination occurs, take appropriate action immediately to prevent accumulative pollution and damage possibly being happened. 1) For cleaning the accessory, refer to the Accessory cleaning equipment. BQ-042-101-01 Revision : 0(2012-12-07) www.bioneer.com...
  • Page 13 Bioneer for service. 2) Complete of instrument decontamination. 3) Fax or e-mail(sales@bioneer.com) the service request form to the customer center. 4) Pack the instrument in the provided packaging, without any accessories or adaptor. Include service request form in the box.

  • Page 14: System Components And Specifications

    Setup Tray 1 ea □ Adaptor 1 ea □ Power cord 1 ea □ (Optional) Multi Puncher (Optional) PCR Preparation Plate for ExiStation ExiStation PCR preparation plate: It is used for system instead of Setup tray. BQ-042-101-01 Revision : 0(2012-12-07) www.bioneer.com...

  • Page 15: Specifications

    Adaptor: AC Input: 100-240VAC, 2.3-0.8A, Electrical (Voltage / Frequency) 50/60Hz, DC Output: 24VDC, 7.5A Instrument: DC Input: 24VDC, 7.5A Network support TCP/IP protocol User interface 320 x 240 touch screen TFT LCD, 18 bit color 3. System Views BQ-042-101-01 Revision : 0(2012-12-07) www.bioneer.com...

  • Page 16: Installing The Exiprep ™16 Dx

    Scissors, pocket knife, or box cutter ExiPrep ™16 Dx 3. Unpacking the Save the packing materials and box in case you need to ship the instrument to Bioneer for service. ExiPrep To unpack the ™16 Dx: a) Cut the straps securing the instrument box.

  • Page 17: Remove Locking Screw

    2. Remove the Holding plate (B) from the rail and the Syringe Block. 3. Remove the Screw (C) from the locking block which immobilizes the belt. 4. Separate the holding block (D, E) from the belt and remove it. BQ-042-101-01 Revision : 0(2012-12-07) www.bioneer.com...

  • Page 18: Power On

    3. Press the power button on the LCD screen to initialize the instrument. A progress bar on the lower portion of the LCD touch-screen will indicate initialization progress. 4. Open the door and remove the Sponge block (F) from the Base Plate after initialization. BQ-042-101-01 Revision : 0(2012-12-07) www.bioneer.com...

  • Page 19: Pc Connect For Existation Tm

    Connect the instrument to the network hub using a LAN cable. 3. Connect a LAN cable between the network hub and the ExiStation PC which has the ™ Manager Software installed. Installation of the instrument is complete. BQ-042-101-01 Revision : 0(2012-12-07) www.bioneer.com...

  • Page 20: Dna/Rna Extraction

    From the ‘Menu’ screen, click ‘MISC SET’.  The base plate moves back to the initialization position (inside the instrument). NOTE ExiStation “Load” button of S/W perform the same function with “MISC SET” button. BQ-042-101-01 Revision : 0(2012-12-07) www.bioneer.com...

  • Page 21: Sample Preparation

    6. Check the position of the diagnosis kit tubes in the rack. Place the Protection Cover (F) on to the rack until it clicks into place.  DO NOT press too hard on the Protection Cover. BQ-042-101-01 Revision : 0(2012-12-07) www.bioneer.com...
  • Page 22 11. Insert the sample into the sample tube. 12. Place the sample tube rack from step 11 onto the Setup Tray. 13. Place the Waste Tray on the Setup Tray (between the tube rack and the Buffer Cartridge ②. BQ-042-101-01 Revision : 0(2012-12-07) www.bioneer.com...

  • Page 23: Run (Not Used Pc)

    5. Verify the ‘Prep Type’ and ‘Sample SRC’ of the three-digit code you have entered. 6. Press the ‘Enter’ button to access the ‘elution volume’ selection menu. BQ-042-101-01 Revision : 0(2012-12-07) www.bioneer.com...
  • Page 24 Select ‘Yes’ to terminate the run, or ‘No’ to cancel the stop and proceeds with the extraction run.  You may select ‘PAUSE’ to temporarily stop the run and ‘RUN’ to resume. BQ-042-101-01 Revision : 0(2012-12-07) www.bioneer.com...
  • Page 25 18. Remove the contamination protection accessory (Tip protector, Contamination shield). Detail can be found in page 19. Press the ‘Store On’ button to turn the Cooling Block and cooling fan off. BQ-042-101-01 Revision : 0(2012-12-07) www.bioneer.com...
  • Page 26 Be careful with the Aluminum sheet of the Protection Cover. 6. Place the Disposable tip rack on the base plate. 7. Push the Base plate all the way inside the instrument and close the door. BQ-042-101-01 Revision : 0(2012-12-07) www.bioneer.com...

  • Page 27: Exiprep Tm 16 Dx Setup

    Once the initialization has completed successfully, the LCD touch-screen will display the MENU as shown below.  Please contact Bioneer Customer Service or your local sales representative if the initialization progress bar does not change for over 5 minutes during initialization or if the MENU screen does not appear after initialization.
  • Page 28 Refer to the code list within this Manual to select the three-digit code(page applicable to your desired nucleic acid and sample source type.  ExiStation Do not use this menu when operating the instrument through the ™ Manager Software. BQ-042-101-01 Revision : 0(2012-12-07) www.bioneer.com...
  • Page 29 LCD touch-screen. To cancel the sterilization process, press the ‘UV lamp’ button again.  ExiStation You may also use the ™ Manager Software to toggle the UV sterilization function. BQ-042-101-01 Revision : 0(2012-12-07) www.bioneer.com...
  • Page 30 This icon is for testing each motor initialization and heater block Temperature.  Tip Out  This icon is for removing the Disposable Tips from the instrument Syringe Block. Pressing this icon will release the tips immediately. BQ-042-101-01 Revision : 0(2012-12-07) www.bioneer.com...

  • Page 31: Registering A New User

    If the login option is enabled, non-registered use will have limited access to the instrument.  Do not forget your user ID. ※ Up to 50 users can be registered. You can manage non-used user IDs using the administrator menu (page 32). BQ-042-101-01 Revision : 0(2012-12-07) www.bioneer.com...

  • Page 32: Viewing Run History

    Work: An abbreviation of sample source and protocol type selected for that run.  Status: Instrument report on whether nucleic acid extraction was successfully completed(OK), stopped during the run (Abort), or cancelled by the user (Canceled). BQ-042-101-01 Revision : 0(2012-12-07) www.bioneer.com...

  • Page 33: Managing The Login Mode

    ※ Entering an invalid user ID three consecutive times with user login mode enabled will shut down the system. Press the ‘Power’ icon on the LCD touch-screen to restart. BQ-042-101-01 Revision : 0(2012-12-07) www.bioneer.com...

  • Page 34: Managing The Automatic Uv-Sterilization Mode

    (UV Mode ON) appears and the UV lamp icon will turn yellow.  Press the ‘UV lamp’ button again to disable the mode. A popup prompt (UV Mode OFF) appears and the UV Lamp icon will turn white. BQ-042-101-01 Revision : 0(2012-12-07) www.bioneer.com...

  • Page 35: Configuring The System

    Press the ‘ok’ button to save the adjusted brightness level. The previous menu will be displayed when the new brightness setting is successfully applied.  To return to the previous menu without saving the adjustments, press the ‘up’ button. BQ-042-101-01 Revision : 0(2012-12-07) www.bioneer.com...

  • Page 36: Administrator Management

     Registered User: Displays the number of registered users.  Select the user ID you wish to delete and press ‘ok’ to confirm deletion.  Use the ‘back’ or ‘next’ buttons to navigate the pages. BQ-042-101-01 Revision : 0(2012-12-07) www.bioneer.com...
  • Page 37 Press the ‘ok’ button to save new administrator ID after verifying the new administrator ID.  You may now use the new administrator ID to delete user IDs or setup and configure the system.  Do not forget new administrator ID. BQ-042-101-01 Revision : 0(2012-12-07) www.bioneer.com...

  • Page 38: Updating The Exiprep Tm 16 Dx

     Please refer to the FAQ in our homepage or contact Bioneer Service Center if updating does not progress as described below or you have questions. NOTE: This program NOT included with the instrument. If you want to the program, you have to request to us.
  • Page 39 15. Disconnect the LAN cable from the computer and the instrument. 16. Reboot the instrument and reconnect the LAN cable. ※ The IP address may change depending on the installation site and PC. BQ-042-101-01 Revision : 0(2012-12-07) www.bioneer.com...
  • Page 40 4. Click the ‘Use the following IP address’. 5. Enter the IP address, subnet mask as follows: IP address: 10. 10. 104. 38 Subnet mask: 255. 255. 192. 0 6. Click ‘OK’ to complete the network connection settings. BQ-042-101-01 Revision : 0(2012-12-07) www.bioneer.com...

  • Page 41: Dna/Rna Extraction Program Number List

    Genomic DNA Bone Total RNA Bone Genomic DNA Dried blood spot Total RNA Dried blood spot Genomic DNA Soil Total RNA Soil Genomic DNA Hair Total RNA Hair Genomic DNA Cell supernatant Total RNA Cell supernatant BQ-042-101-01 Revision : 0(2012-12-07) www.bioneer.com...
  • Page 42 DNA Bone marrow mRNA Bone viral DNA Bone mRNA Dried blood spot viral DNA Dried blood spot mRNA Soil viral DNA Soil mRNA Hair viral DNA Hair mRNA Cell supernatant viral DNA Cell supernatant BQ-042-101-01 Revision : 0(2012-12-07) www.bioneer.com...
  • Page 43 Bone viral RNA Dried blood spot viral DNA/ RNA Dried blood spot viral RNA Soil viral DNA/ RNA Soil viral RNA Hair viral DNA/ RNA Hair viral RNA Cell supernatant viral DNA/ RNA Cell supernatant BQ-042-101-01 Revision : 0(2012-12-07) www.bioneer.com...
  • Page 44 Fully Automated Nucleic Acid Extraction System Target Sample source Target Sample source endA Plasmid DNA (+) strain Protein His-Tag endA Plasmid DNA (-) strain Target Sample source Fragment DNA Gel slice Fragment DNA PCR product Fragment DNA Enzymatic reaction BQ-042-101-01 Revision : 0(2012-12-07) www.bioneer.com...

  • Page 45: Troubleshooting

    Check if the base plate is placed correctly. After holding the door to open and when releasing the door to be closed, check if the door is closed automatically by the spring. Request for After Service. BQ-042-101-01 Revision : 0(2012-12-07) www.bioneer.com...
  • Page 46 When the block is being moved, check if any residues or doesn’t move. accessories are stuck. Check if the cartridge is inserted correctly. Check if ‘Stop’ or ‘Pause’ button is pressed. Request for After Service. BQ-042-101-01 Revision : 0(2012-12-07) www.bioneer.com...
  • Page 47 There is a burning smell in the Disconnect the power and unplug the power connector system. immediately. Request for After Service. UV Lamp does not work. Check if the door is closed completely. Request for After Service. BQ-042-101-01 Revision : 0(2012-12-07) www.bioneer.com...
  • Page 48 2. Check to see if the software is executed twice. 3. Restart the instrument and software. 4. Request service from your dealer. buttons pictures 1. Erase and re-install the software. displayed within the software. BQ-042-101-01 Revision : 0(2012-12-07) www.bioneer.com...

  • Page 49: Warranty

    This instrument is warranted by Bioneer against manufacturing defects in materials and workmanship for a limited warranty period of one (1) year from the date you received your product. Bioneer will either (1) repair the product at no charge if a hardware defect is found or (2) exchange the product if the same hardware defect arises more than three times during the limited warranty period.
  • Page 50 Date of request Date of purchase * Please list one service issue or concern per line Issue 1 Issue 2 Service Issue Issue 3 Issue 4 Name Company Customer Name Information Contact Phone: Fax: E - mail BQ-042-101-01 Revision : 0(2012-12-07) www.bioneer.com...

Table of Contents