Related Manuals for Bioneer ExiPrep K-3200-CB
Summary of Contents for Bioneer ExiPrep K-3200-CB
- Page 1 ExiPrep™ Beef Genomic DNA Kit Cat. No. K-3200-CB © Copyright 2022 BIONEER Corporation. All rights reserved.
- Page 2 Kit for the extraction of beef genomic DNA User Guide K-3200-CB Version No.: 4 (2022-06-10) Please read all the information in booklet before using the unit BIONEER Corporation Bioneer Global Center, 71, Techno-2-ro, Yuseong-gu, Daejeon, 34013, Republic of Korea Tel: 1588-9788 Email: sales@bioneer.co.kr www.bioneer.com...
- Page 3 Material Safety Data Sheet (MSDS). Warranty and Liability All BIONEER products undergo extensive Quality Control testing and validation. BIONEER guarantees quality during the warranty period as specified, when following the appropriate protocol as supplied with the product. It is the responsibility of the purchaser to determine the suitability of the product for its particular use.
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Page 4: Table Of Contents
ExiPrep™ Beef Genomic DNA Kit Contents Product Information ……………………………………………………………………………. Components ………………………………………………………………………………….. Storage ……………………………………………………………………………………… Specifications………………………………………………………………………………… Precautions ………………………………………………………………………………….. Introduction ……………………………………………………………………………………… Product Description …………………………………………………………………………. Principle ………………………………………………………………………………………. Features & Benefits …………………………………………………………………………. Components of Buffer Cartridges ………………………………………………………….. Experimental Procedures ……………………………………………………………………… Preparing Sample from Animal Tissues …………………………………………………… Loading the Kit to the Instrument ………………………………………………………….. -
Page 5: Product Information
Buffer Cartridges (RNase A) and 2 ml screw cap tubes (Proteinase K). They can be stored at room temperature up to 2 years without any reduction in activity, provided they remain unopened. Once dissolved, enzymes should be stored at -20°C for up to 6 months. BQ-042-101-01 Revision : 4 (2022-02-14) www.bioneer.com... -
Page 6: Specifications
All of the plastic products and buffers in this kit are provided under nuclease-free condition, hence, please be careful not to contaminate any part of the kit with nuclease. BQ-042-101-01 Revision : 4 (2022-02-14) www.bioneer.com... -
Page 7: Introduction
16 extracted samples automatically. The process does not require phenol/chloroform extraction and ethanol precipitation. DNA extracted through this kit can be used for a variety of applications, including: gene cloning, PCR, Real-time PCR, genetics, and SNP genotyping. BQ-042-101-01 Revision : 4 (2022-02-14) www.bioneer.com... -
Page 8: Principle
Any salts and precipitates are eliminated by washing buffer, and captured genomic DNA is eluted in an elution buffer or nuclease-free water. BQ-042-101-01 Revision : 4 (2022-02-14) www.bioneer.com... -
Page 9: Features & Benefits
Reproducible: Uses fully automatic nucleic acid extraction equipment, and reproducible results can be obtained. Efficient: Contains all required consumables such as Disposable Filter Tips and Elution Tubes. Ready-to-use: Extracted DNA is ready-to-use for various application. BQ-042-101-01 Revision : 4 (2022-02-14) www.bioneer.com... -
Page 10: Components Of Buffer Cartridges
ExiPrep™ Beef Genomic DNA Kit Components of Buffer Cartridges Figure 1. Position of reagents, tubes, and starting material in Cartridges/ Elution Tube Rack of ExiPrep™ Beef Genomic DNA Kit (K-3200-CB). BQ-042-101-01 Revision : 4 (2022-02-14) www.bioneer.com... -
Page 11: Experimental Procedures
4. Centrifuge at 13,000 rpm for 5 minutes to remove any precipitates. 5. Transfer the cleared lysate to a new 1.5 ml tube (not provided). 6. Proceed immediately to “Loading the Kit to the Instrument” on page 8. BQ-042-101-01 Revision : 4 (2022-02-14) www.bioneer.com... -
Page 12: Loading The Kit To The Instrument
2. Load 200 µl of samples prepared in page 7 into ‘Sample loading well’ of Cartridge ①. * Note: Be careful not to contaminate any other wells. 3. Open the door of ExiPrep™ 16 and pull out the baseplate completely. BQ-042-101-01 Revision : 4 (2022-02-14) www.bioneer.com... - Page 13 Rack is slotted so it can only be placed in the correct orientation. * Note: Make sure the direction of the Elution Tube caps laid out as on the left when inserting into the Elution Tube Rack. BQ-042-101-01 Revision : 4 (2022-02-14) www.bioneer.com...
- Page 14 ‘Enter’ button. Prep Type: Genomic DNA Sample SRC: Animal tissue 14. Select the desired elution volume from the touch screen. 15. Press the ‘ok’ button to move to the next step. BQ-042-101-01 Revision : 4 (2022-02-14) www.bioneer.com...
- Page 15 20. Remove all components used in the experiment, and choose 1, 2, or ok. * Note: If you want to quit and press the ‘ok’ button, the UV lamp will be turned on automatically. BQ-042-101-01 Revision : 4 (2022-02-14) www.bioneer.com...
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Page 16: Troubleshooting
Furthermore, magnetic nanobeads cannot bind genomic DNA in elution buffer, though it may affect readings on a spectrophotometer. Magnetic nanobeads that are carried over can be easily separated by centrifugation at 13,000 rpm for 1 minute. BQ-042-101-01 Revision : 4 (2022-02-14) www.bioneer.com... -
Page 17: Ordering Information
Ordering Information Description Cat. No ExiPrep™ Beef Genomic DNA Kit K-3200-CB Related Products Description Cat. No A-5010 ExiPrep™16 ExiProgen™ A-5041 Proteinase K Powder KB-0111 BQ-042-101-01 Revision : 4 (2022-02-14) www.bioneer.com... -
Page 18: Explanation Of Symbols
ExiPrep™ Beef Genomic DNA Kit Explanation of Symbols Batch Biological Catalog Caution Code Risks Number Consult Contains Do not Instructions Sufficient for Manufacturer Re-use For Use <n> tests Research Temperature Use-by Limitation Date Only BQ-042-101-01 Revision : 4 (2022-02-14) www.bioneer.com... - Page 19 © Copyright 2022 BIONEER Corporation. All rights reserved.