Related Manuals for Bioneer ExiPrep 96 Lite
Summary of Contents for Bioneer ExiPrep 96 Lite
- Page 1 ExiPrep ™96 Lite ExiPrep ™96 Lite Automated Nucleic Acid Extraction System USER GUIDE A-5250 35℃ 15℃ Version 7.0 (2021-06-18) © Copyright 2021 BIONEER Corporation. All rights reserved. REVISION 0 (2018-07-05)
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Page 3: Table Of Contents
Downloading the Operation Logs ................44 MAINTENANCE ......................46 Changing the Magnetic Rods ..................46 Updating the Software ..................... 47 Self-Test ......................48 Fuse Replacement ....................50 Instrument Transport ....................51 Storage Requirements ..................... 51 Backup Power ....................... 52 Instrument Disposal ....................52 www.bioneer.com... - Page 4 WARRANTY ......................61 Responsibility ......................61 User Guide Amendments ..................61 KEY TO SYMBOLS ....................62 APPENDIX A: Certificate of Decontamination ..............65 APPENDIX B: Service Request Form ................67 ExiPrep APPENDIX C: ™96 Lite Maintenance Log ..............69 www.bioneer.com...
- Page 5 Figure 14. Standard Protocol Screen ................16 Figure 15. UV Setup Screen ..................17 Figure 16. Setup Screen – General Tab ................18 Figure 17. Setup Screen – Log Tab ................19 Figure 18. Setup Screen - Device Tab ................20 www.bioneer.com...
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Page 7: Intended Use
During the binding step, the free nucleic acids stick to the silica surface of the magnetic beads. A wash removes the impurities, and the final elution step isolates the nucleic acids from the beads providing pure nucleic acids in the storage buffer. Figure 1. Nucleic acid extraction process www.bioneer.com... -
Page 8: Product Information
ExiPrep interchangeable magnetic rods. ™96 Lite can be used with 24- or 96-well plates depending ExiPrep on the type of kit. Only ™96 kits manufactured by BIONEER are compatible with the ExiPrep ™96 Lite instrument. Product Advantages • High throughput that extracts nucleic acids from up to 96 specimens at once. -
Page 9: Product Specifications
0 - 40°C (32 – 104°F), in shipping container Storage temperature range 5 - 40°C (41 – 104°F) Storage humidity range 20 - 80%, no condensation Software Specifications Contact Customer Support for questions Software version relating to software version. www.bioneer.com... - Page 10 30 – 90℃ (86 - 194℉) Cooling block temperature 4 - 90℃ (39.2 - 19℉) U.S. and Canadian Safety Standards Standard for Electrical Equipment for Measurement, Control and Laboratory Use Part 1: General Requirements, UL 61010-1:2012, 3 Ed. 5/11/12, CSA C22.2 No. 61010-1-12 www.bioneer.com...
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Page 11: Product Components
™96 Lite package are shown below. The 8-well magnetic rods may be purchased separately. ExiPrep Figure 3. ™96 Lite Components ExiPrep Table 1. ™96 Lite Components Components Cat. No. Qty. ExiPrep ™96 Lite A-5250 32-well Magnetic Rod A-5250-A2 Power Cord User Guide 8-well Magnetic Rod (Optional) A-5250-A4 www.bioneer.com... -
Page 12: Functional Description - Hardware
Connection for loading software and protocols into the Rear USB Port instrument and transferring operation logs from the instrument On (|): Allows power to the instrument and places it in Main Power Switch Standby mode Off (0): Turns off power to the instrument www.bioneer.com... -
Page 13: Table 3. Description Of Exiprep ™96 Lite External Components - Sensors
Table 3. Description of ™96 Lite External Components – Sensors Component Description Deactivates the UV light if the instrument door is UV Lamp Safety Sensor opened during sterilization Stops the operation if the instrument door is opened Door Sensor during processing www.bioneer.com... -
Page 14: Table 4. Description Of Exiprep ™96 Lite Internal Components - View A
Prevents contamination of Plate 3 Contamination Shield 2 Prevents contamination of Plate 2 Contamination Shield 1 Prevents contamination of Plate 1 Incubation Heating Block Facilitates the isolation of nucleic acids Heats or cools cartridge 7 to stabilize the samples Elution Block during elution www.bioneer.com... -
Page 15: Table 5. Description Of Exiprep ™96 Lite Internal Components - View B
Isolates nucleic acids using magnetic force Plate 3 Carries buffer cartridges 5 and 6 back and forth Plate 2 Carries buffer cartridges 3 and 4 back and forth Plate 1 Carries buffer cartridges 1 and 2 back and forth www.bioneer.com... -
Page 16: Table 6. Description Of Exiprep ™96 Lite Internal Components - Plate 1
™96 Lite Internal Components – Plate 1 Component Description Plate 1 Carries cartridges 1 and 2 back and forth Cartridge Zone 1 Location for placement of cartridge 1 containing samples Cartridge Zone 2 Location for placement of cartridge 2 www.bioneer.com... -
Page 17: Table 7. Description Of Exiprep ™96 Lite Internal Components - Plate 2
Table 7. Description of ™96 Lite Internal Components - Plate 2 Component Description Plate 2 Carries cartridges 3 and 4 back and forth Cartridge Zone 3 Location for placement of cartridge 3 Cartridge Zone 4 Location for placement of cartridge 4 www.bioneer.com... -
Page 18: Table 8. Description Of Exiprep ™96 Lite Internal Components - Plate 3
Table 8. Description of ™96 Lite Internal Components – Plate 3 Component Description Plate 3 Carries buffer cartridges 5 and 6 back and forth Cartridge Zone 5 Location for placement of cartridge 5 Cartridge Zone 6 Location for placement of cartridge 6 www.bioneer.com... -
Page 19: Table 9. Description Of Exiprep ™96 Lite Internal Components - Elution Block
Controls the temperature of buffer cartridge 7 to stabilize the samples during the elution step Buffer Cartridge Zone 7 Location for placement of cartridge Loads buffer cartridge 7 in zone 7 where extracted nucleic acids are deposited at the end of the process www.bioneer.com... -
Page 20: Functional Description - Software
Setup Opens Setup screen Remaining Time Each tap toggles the display to show Remaining Time, Running Time, and Finish Time Step Displays the current step of the operation Starts the operation Pauses the operation Stops the operation www.bioneer.com... -
Page 21: Table 11. Description Of The Assign Sample Screen Components
Allows input of sample IDs manually or by barcode scanner INIT Clears (initializes) the sample ID list LOAD Loads the previously saved sample IDs Saves sample IDs and returns the display to the Home screen Returns the display to the Home screen without saving the sample IDs www.bioneer.com... -
Page 22: Table 12. Description Of The Standard Protocol Screen Components
Allows the selection of the available standard protocols available Washing Times & Elution Disabled from making changes during use of standard Volume protocols. Loads the selected protocol and returns the display to the Home screen Returns the display to the Home screen without saving www.bioneer.com... -
Page 23: Table 13. Description Of The Uv Setup Screen Components
Allows the user to turn the UV lamp on/off manually 15 Minutes Turns on the UV lamp for the default 15-minute the sterilization process Starts the selected UV process Returns the display to the Home screen without performing the sterilization process www.bioneer.com... -
Page 24: Table 14. Description Of The Setup Screen - General Tab Components
Table 14. Description of the Setup Screen - General Tab Components Component Description SW Update Allows the software to be updated when a USB drive with the updated program is connected Instrument Information Displays the instrument name, software version, and firmware version Contact Information Displays BIONEER’s contact information www.bioneer.com... -
Page 25: Table 15. Description Of The Setup Screen - Log Tab Components
Double tap the log name to display the operation time, protocol information, and the sample IDs. A maximum of 100 logs can be stored. Saves the selected log file to a connected USB drive Accessible only to BIONEER technicians www.bioneer.com... -
Page 26: Table 16. Description Of The Setup Screen - Device Tab Components
Turns the dry heater off and on Peltier Off/On Turns the elution heater off and on TopHeat Turns the incubation heater off and on Date Time Displays the date and time and allows the date and time to be adjusted www.bioneer.com... -
Page 27: Instrument Status Indicators
The status of the instrument is indicated by the color of the LED status indicator surrounding the Power button. Table 17. Instrument Status Indicator Color Definitions Color Description Error Yellow Initializing Green Ready Cyan Operating Blue Standby White Paused www.bioneer.com... -
Page 28: Warnings And Precautions
Do not touch power cords with wet hands. This may cause electric shock. • Do not place objects that may interfere with instrument operation on or near the instrument. • Do not disassemble or modify the instrument. It may cause fire, electric shock, or equipment failure, and may void the warranty. www.bioneer.com... -
Page 29: Installation Precautions
96 Lite for any purpose other than its intended use as a medical device to for extracting nucleic acid as stated in this User Guide. • Use only the accessories and cartridges provided by BIONEER when using this instrument. • Cartridges must contact the heating and cooling blocks completely during the cartridge installation. -
Page 30: Uv Lamp Precautions
• If liquid waste is spilled inside the instrument, the instrument must be disassembled and cleaned to eliminate contamination and ensure normal instrument operation. Contact BIONEER Technical Support for service. Cleaning Precautions •... -
Page 31: Storage And Maintenance Precautions
Do not store the instrument in a humid place. Instrument breakdown due to humidity classifies as “break down due to immersion” and is not covered by the warranty. • Do not modify or disassemble the instrument other than as directed in this User Guide as this may void the warranty. www.bioneer.com... -
Page 32: Installation
Product label • Package conditions If there are any abnormalities with the items mentioned above, contact BIONEER Customer Service with a detailed description of the issue. Installation Requirements Review the materials list and location requirements listed below and prepare the site before installing the instrument. -
Page 33: Unpacking
Figure 19. Unpacking the ™96 Lite 1. Use scissors to cut the straps on the packaging. 2. Remove the lid and extract the box of accessories. 3. Remove the protection packaging. 4. Carefully lift the instrument onto the bench. www.bioneer.com... -
Page 34: Removing The Transportation Lock
To loosen the bolts, turn counterclockwise. 3. Remove the transportation lock. 4. Do not put the bolts back into the instrument. They interfere with the operation and may damage the instrument. Front View Rear View www.bioneer.com... -
Page 35: Connecting The Power
Press the Power button for 1 second to turn on the instrument and ensure the instrument Initializes without any problems. • Ensure the LCD screen is illuminated. • Ensure the touchscreen functions properly. If there are any abnormalities with the items mentioned above, contact BIONEER Customer Support with a detailed description of the issue. www.bioneer.com... -
Page 36: Operation
Wear powder-free disposable gloves when handling cartridges and internal instrument components. 1. Ensure the pawer cord is connected to an electrical outlet and to the instrument. Do NOT turn on the main power switch as the back of the instrument. 2. Open the door of the instrument www.bioneer.com... - Page 37 4. Pull out Plate 1 until it the belt bracket comes in contact with the pully on the left side. If the pulley and bracket do not make contact, the instrument may not function correctly. www.bioneer.com...
- Page 38 10. Load cartridge 7 on the elution heating block. 11. Push down on the magnetic rod cover plate until it is below the magnetic rods. 12. Insert 3 magnetic rod covers into the slot of the magnetic rod cover plate. www.bioneer.com...
- Page 39 13. Ensure the cartridges and magnetic rod covers are in place, then close the instrument door. 14. Turn on the Main Power Switch on the back of the instrument. 15. Turn on the Power button on the front of the instrument. www.bioneer.com...
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Page 40: Starting The Extraction Process
Standard Protocol screen will be displayed. 2. Select the desired protocol and tap . The selected protocol will load and the Home screen will be displayed. 3. Tap at the bottom of the screen. A Check List window will be displayed. www.bioneer.com... - Page 41 The operation time, protocol information and sample IDs are recorded in the operation log and may be accessed by selecting Setup on the Home screen and then selecting Log. www.bioneer.com...
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Page 42: Removing The Cartridges
4. Unload the magnetic rod covers. 5. Cover cartridges 1 and 2 and unload them from Plate 1. Make sure each cartridge is securely covered before unloading it from the instrument. Buffers may spill from unclosed or partially closed cartridges. www.bioneer.com... - Page 43 10. Tap the upper part of the Plate button to retract Plate 3. 11. Dispose of cartridges 1 through 6 according to all applicable local and national safety procedures and regulatory guidelines. 12. Close the door of the instrument. www.bioneer.com...
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Page 44: Cleaning And Sterilizing The Instrument
3. Tap Cover Down to lower the magnetic rod cover plate. 4. Remove the rod covers from the magnetic cover plate and set them aside. 5. Remove the knobbed bolts on the top of the magnetic rod plate by turning the knobs counterclockwise. www.bioneer.com... - Page 45 Do not place magnetic rods near any electronic media as the magnets may erase the data. 9. Clean the magnetic rod cover plate with cleaning solution. 10. Tap Cover Up. 11. Tap Cap3 Out to extend contamination shield 3. www.bioneer.com...
- Page 46 Do not use excessive pressure when cleaning. 13. Tap Cap3 In to retract contamination shield 3. 14. Repeat steps 11-14 tapping Cap2 Out and Cap2 In to access contamination shield 2 for cleaning. www.bioneer.com...
- Page 47 20. Tap the upper part of the Plate button to extend Plate 2. 21. Clean Plate 2 with all three solutions. 22. Tap the upper part of the Plate button again to retract Plate 2 and extend Plate 1. 23. Clean Plate 1 with all three solutions. www.bioneer.com...
- Page 48 Do not over-tighten the knobs as this will strip the threading on the bolts. 28. Slide the clean the rod covers into the magnetic rod cover plate. 29. Tap Cover Up to raise the magnetic rod cover plate. 30. Tap to close the Setup screen. www.bioneer.com...
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Page 49: Uv Sterilization
. The UV Progress window with a count-down timer will be displayed. To select a different amount of time for sterilization, select Manual, enter the amount of time, in minutes, and tap If started manually, the UV sterilization process must be stopped manually as well. www.bioneer.com... -
Page 50: Downloading The Operation Logs
3. Select the Log tab. 4. Select the log you wish to download. To view the contents of a log, double-click the log name. 5. With the log name highlighted, tap to copy the selected file to the USB drive. www.bioneer.com... - Page 51 ExiPrep ™96 Lite 6. Press the Power button for 1 second to place the instrument on standby. The status indicator will turn blue. 7. Turn off the instrument using the main power switch on the back of the instrument. www.bioneer.com...
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Page 52: Maintenance
7. Reinsert the knobbed bolts into the magnetic rod plate regardless of whether rods are in that section of the plate and turn clockwise to tighten. Do not over-tighten the knobs as this will strip the threading on the bolts. www.bioneer.com... -
Page 53: Updating The Software
1. From the Home screen tap Setup. The Setup screen will be displayed. 2. Select the General tab. The instrument name, software version, firmware version and BIONEER contact information will be displayed. 3. Insert a USB drive containing the updated software in the USB port on either the front or the back of the instrument. -
Page 54: Self-Test
If there is a problem with instrument operation or abnormal conditions are observed, perform the self- test process below. 1. From the Home screen tap Setup. The Setup screen will be displayed. 2. Select the Device tab. 3. Tap Self-test. 4. The instrument will run the self-test sequence. www.bioneer.com... - Page 55 ™96 Lite 5. The areas with problems will display Fail as the result. The areas without problems will display Pass. If Fail is displayed, contact BIONEER Technical Support. 6. If all areas show Pass the instrument is ready for use.
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Page 56: Fuse Replacement
<그림 1. 퓨즈 교체 2> 5. Connect the power cord and turn on (|) the Main Power Switch 6. Ensure the LED status indicator around the Power button on the front of the instrument turns blue. www.bioneer.com... -
Page 57: Instrument Transport
™96 Lite Instrument Transport Follow the steps below to move the instrument to a different location. For technical assistance please contact BIONEER Technical Support. 1. Remove all cartridges and magnetic rod covers. 2. Disconnect the power cable. 3. Disconnect the power cable. -
Page 58: Backup Power
Sine wave Operation Temperature 0 - 40°C Operation Humidity 0 - 95% Instrument Disposal BIONEER only accepts decontaminated instruments for safety reasons. After decontaminating the instrument, complete the “Certificate of Decontamination” and contact BIONEER Technical Support for return information. www.bioneer.com... -
Page 59: Troubleshooting
This section describes the problems that may occur when the instrument is in use and the corresponding actions that should be taken. If the action listed does not solve the problem, or if the problem is not listed below, contact BIONEER Technical Support. Problem... - Page 60 1. Make sure the progress bar is moving on the LCD touchscreen. middle of the process. 2. If the progress bar is not moving, check to see if tapped, and if so, resume, operation by tapping 3. If the instrument will not resume, contact Technical Support. www.bioneer.com...
- Page 61 2. Check if there is any problem with the protocol file. 3. Try importing the protocol again. 4. If the protocol still cannot be imported, contact Technical Support. Burning smell coming 1. Unplug the power cord immediately and contact Technical from the instrument Support. www.bioneer.com...
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Page 62: Frequently Asked Questions
™96 Lite can extract nucleic acids from blood, swabs, sputum, urine, etc. Before loading viscous samples, check their viscosity using a pipette. If the sample cannot be pipetted, further pre-treatment is required. BIONEER recommends using our EZ solution. Contact Customer Support for purchase. - Page 63 How much sample should be dispensed into each cartridge? As a general rule, 96-well extraction kits use 200 – 400 µL, but BIONEER recommends using 200 µL for diagnostic purposes, and 24-well extraction kits (cfDNA kits) use 4 ㎖. However, follow the...
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Page 64: Technical Support
7. Contact BIONEER Technical support before shipping to obtain specific shipping information. Once your package is received by BIONEER, service may take up to three weeks depending on the instrument. Failure to include the signed Certificate of Decontamination or the completed Service Request Form will result in delays. -
Page 65: Ordering Information
Automated nucleic acid extractor (16 samples/test) Kits Cat No. Product Description ExiPrep K-4614 ™ 96 Viral Viral DNA or RNA extraction from various specimens DNA/RNA kit ExiPrep K-4611 ™ 96 Genomic Genomic DNA extraction from various specimens DNA kit www.bioneer.com... -
Page 66: Legal Information
Disclaimer BIONEER Co., Ltd reserves the right to change product specifications and services at any time for technical improvement of products. For technical problems related to product quality and test results, if necessary, contact the customer service center (ds@bioneer.com) with analysis information or relevant test data. -
Page 67: Warranty
BIONEER is excluded. Responsibility BIONEER does not assure liability for use of the product apart from its intended purpose, as described in the appropriate and applicable User Guide. User Guide Amendments This user guide is subject to change without prior notice due to continuous revision. -
Page 68: Key To Symbols
To minimize the effect of electric or electronic instruments on the environment, follow the regulations on proper disposal of such items. European Union customers Call your local Europe office for Bioneer instruments pick-up and recycling. Manufacturer Date of Manufacture Authorized representative in the European Community... - Page 69 Indicates the on/off position of the main power switch. Indicates ground terminal for the main protective ground of the device. Indicates a terminal that receives or supplies alternating current or voltage. Indicates a terminal that receives or supplies direct current or voltage. www.bioneer.com...
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Page 71: Appendix A: Certificate Of Decontamination
□ Biological materials □ Radioactive materials Additional Information (if any) Procedure taken for decontamination Date Name/signature If any radioactive material was used, a signature of the radiation safety manager is needed to certify the removal of radioactive contamination. Date Name/signature www.bioneer.com... - Page 72 www.bioneer.com...
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Page 73: Appendix B: Service Request Form
APPENDIX B: Service Request Form Service Request Form Product ExiPrep ™96 Lite Catalog No. A-5251 Serial No. Date of Request Date of Purchase Date Part Description Problem Additional Information (if needed) Name of Institution Address Department Customer Name Contact Phone Information E-mail www.bioneer.com... - Page 74 www.bioneer.com...
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Page 75: Appendix C: Exiprep ™96 Lite Maintenance Log
Check temperature of the incubation heating block Check temperature of the elution block Check home position * Required Materials: Distilled Water, 70% Ethanol, 1% Bleach (or 2% Acetic Acid), Nucleic Acid Degradation Solution, Swab, Lint-Free Cloth/Paper Towel, Powder-Free Disposable Gloves www.bioneer.com Revision... - Page 76 www.bioneer.com...
- Page 77 BIONEER Inc. Address 155 Filbert St. Suite 216 Oakland, CA 94607, USA Telephone +1-877-264-4300 (Toll-free) +1-510-865-0350 E-mail order.usa@bioneer.com us.bioneer.com BIONEER R&D Center Address Korea Bio Park BLDG #B-702, 700 Daewangpangyo-ro, Bundang-gu, Seongnam-si Gyeonggi-do, 13488, Republic of Korea Telephone +82-31-628-0500 +82-31-628-0555 E-mail sales@bioneer.co.kr...
- Page 78 BIONEER CORPORATION 8-11, Munpyeongseo-ro, Daedeok-gu, Daejeon, 34302, Republic of Korea MT Promedt Consulting GmbH Altenhofstr. 80 D-66386 St. Ingbert, Germany Phone: +49 6894 581020 A-5250...