Thermo Scientific ETD Getting Started Manual page 72

B
Angiotensin I Solutions
56 MS/ETD System Getting Started Guide
Other potentially hazardous chemicals used in the following procedures in this section
include:
• Glacial Acetic acid
• Methanol
Handle these chemicals according to their MSDS guidelines.

To prepare an Angiotensin I stock solution
1. Remove the 1 mg vial of Angiotensin I from the accessory kit.
2. Add the following to the 1 mg of Angiotensin I:
382 μL of water
382 μL of methanol
8 μL of glacial acetic acid
3. Mix the solution thoroughly.
 To prepare an Angiotensin I sample solution
1. Pipet 100 μL of the stock solution (1 nmol/μL) of Angiotensin I into a clean,
polypropylene microcentrifuge tube.
2. Add 900 μL of 50:50 methanol/water (0.1% acetic acid) to the tube.
3. Mix the solution (100 pmol/μL) thoroughly.
4. Pipet 19.8 mL of 0.1% acetic acid 50:50 methanol/water into a clean, 20 mL glass
scintillation vial.
5. Add 200 μL of the 100 pmol/μL solution into the scintillation vial to bring the final
volume to 20 ml.
6. Mix the solution (1 pmol/μL) thoroughly.
7. Store this sample solution (1 pmol/μL) in a refrigerator until it is needed.
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