Data Dependent NL MS
Thermo Scientific
13. In the Tune Plus window, click the Save
14. Browse to the
:\Xcalibur\methods folder, and specify a file name.
15. Click Save.
3
Experiment
To run a Data Dependent NL MS
1. Choose Start > Programs > Xcalibur > Xcalibur
2. Click the Instrument Setup icon
3. In the Available Devices pane, click the LTQ XL MS or LTQ Velos MS icon to open the
New Method page
(Figure 30
4. Under Select ETD Experiment Type, click Data Dependent NL MS3 (ETD) to open
this dialog box
(Figure
The Initialize Method to Repeat the MS2 Event Using ETD check box is selected as the
default setting.
Figure 35. Data Dependent NL MS3 dialog box
5. In the Analyze Top N Peaks box, enter the number of top peaks.
6
(
) button.
3
(ETD) experiment
(Figure 29
on
on
page
44).
35).
Running an Xcalibur Experiment by Using ETD
Data Dependent NL MS
(Figure 28
on
page
42).
page
43).
MS/ETD System Getting Started Guide
3
Experiment
47