Figure 33. Data Dependent Settings Dialog Box; Figure 34. Activation Parameters - Thermo Scientific ETD Getting Started Manual

6
Running an Xcalibur Experiment by Using ETD
Nth Order Double Play (ETD) Experiment
46 MS/ETD System Getting Started Guide
8. (For Scan Event 2 and Scan Event 3 only) Click Settings to open the Data Dependent
Settings dialog box (Figure

Figure 33. Data Dependent Settings dialog box

9. In the left pane, under the Scan Event list, click Activation to see the activation
parameters (Figure 34).
The activation type is automatically set to ETD.

Figure 34. Activation parameters

10. In the Default Charge State box, enter a value of 5 or more.
11. In the Isolation Width (m/z) box, enter a value between 2 and 3.
The Normalized Collision Energy and Activation Q parameters are unavailable because
they are not applicable to ETD.
12. In the Activation Time (ms) box, leave the default value or enter a different value as
described in "Optimizing the Reagent Ion Reaction Time"
33).
on page 38.
Thermo Scientific