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Thermo Scientific VSTAR-ISE Reference Manual

Ph/ise/temperature module
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Thermo Scientific Orion VERSA STAR™
pH/ISE/Temperature Module
Reference Guide

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Summary of Contents for Thermo Scientific VSTAR-ISE

  • Page 1 Thermo Scientific Orion VERSA STAR™ pH/ISE/Temperature Module Reference Guide...
  • Page 2 Ross and the COIL trade dress are trademarks of Thermo Fisher Scientific, Inc. and its subsidiaries. AQUAfast, AQUASensors, BOD AutoEZ, ionplus, KNIpHE, LogR, No Cal, ORION, perpHect, PerpHecT, pHISA, pHuture, Pure Water, Sage, ROSS, ROSS Ultra, Sure-Flow, Titrator PLUS, and TURBO2 are registered trademarks of Thermo Fisher Scientific, Inc.

  • Page 3: Table Of Contents

    Thermo Scientific Orion VERSA STAR™ pH/ISE/Temperature Module Table of Contents Chapter 1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 Chapter 2 Module Overview .
  • Page 4 Chapter 10 Data Storage and Review . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23 Data Storage Settings .

  • Page 5: Introduction

    Chapter 1 Introduction Thank you for your purchase of the Thermo Scientific Orion VERSA STAR™ pH/ISE/Temperature Module. This module is for use with the Thermo Scientific Orion VERSA STAR Advanced Electrochemistry Benchtop Meter. Some key features of this module include: • pH range from -2 to 20 with resolution options of 0.001, 0.01 and 0.1...
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  • Page 7: Module Overview

    The pin tip reference connector is on the lowest panel of the pH/ISE module. 2. Place the pH or ISE, ATC probe and Orion Star stirrer probe (cat. no. 096019, optional) into the electrode arm. 3. Attach the pH or ISE BNC and ATC probe to the pH/ISE meter module. Lower ISE and ATC into solution for conditioning if required. If using the stirrer probe, attach probe to the meter, place into the electrode arm and assign the probe to the channel in the setup menu for instrument settings. 4. Prepare fresh standards daily for calibration. For ISE testing, standards should be at least one decade (10 times) apart. The standards should exceed the expected minimum and maximum sample range. Serial dilution is recommended using calibrated pipettes to accurately prepare calibration standards from a stock solution. 5. Gather reagents, rinse water, and other supplies. For ISE testing, if ISA will be added to the sample, it needs to be added to all the standards prior to calibration to ensure a consistent dilution factor. pH/ISE Module 8 pin mini-DIN for ATC BNC connection with shorting cap Reference pin socket Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module...

  • Page 8: Module Maintenance

    Corresponding mV value Calibration information The information on the display can be customized. Enter the setup menu to make changes. 1. Press Setup. 2. Use the arrow keys to highlight Inst. Settings, press (f3/select) key. 3. Use the arrow key to highlight Display View. Press (f3/select) key. 4. Follow the onscreen prompts to choose what you would like to see on the measurement screen. Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module...

  • Page 9: Setup Menus

    (Default values are in bold in the table below) Electrode ID Input Sample ID Off, Manual, Auto Increment Resolution 0.1, 0.01, 0 .001 Stability Smart, Fast, Medium, Slow Averaging Off, Automatic Smart Read Type AutoRead, Timed, Continuous Buffer Group USA, DIN, Custom Isopotential Value Off, On 7 .000 Linear Regression Off, On Alarm** Limit, CalDue, Set Point ** Limit and set point alarm default set to ‘off’. Cal due alarm set to 12 hours. Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module...

  • Page 10: Read Type Information

    • Auto-Read – The meter will display the measurement as it stabilizes and lock and hold the measurement when it is stable (The AR icon will lock on the screen.) Once the measurement is locked, the meter will automatically export the measurement to the data log, if the data log is enabled in the setup menu, and to a printer or computer, if a printer or computer is connected to the meter and enabled in the setup menu. To take a new measurement, press measure (esc)/1. • Timed – The meter will read and display a measurement at the set time interval that is programmed by the operator. There are two options: By checking/selecting the "Interval" box, at the set time interval, the meter will automatically export the measurement to the data log, if the data log is enabled in the setup menu, and to a printer or computer, if a printer or computer is connected to the meter and enabled in the setup menu. If selected, enter the time interval value in a hours:minutes:seconds format using the numeric keypad and press f3 (select) key. 2. If the "Interval" box is unchecked/not selected, a single timed measurement will be taken. The meter will apply the same saving and exporting conditions as with the set time interval to record and export data, but lock after one timed interval has expired. Press measure (esc)/1 to reset the countdown clock and take a new reading. Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module...

  • Page 11: Isopotential Value

    The default is off which uses the traditional, segmented approach. The segmented approach is the most accurate and precise. More information can be found in the appendix section of this reference guide under the Multipoint Calibration Curve Feature. Selecting “on” for this option results in the use of a single best fit line for the calibration curve, and can be beneficial if pH measurements vary greatly. Low Level Stability This feature adjusts the timing for the calibration points for lower level ISE standards. Setup Menu for Temperature All of the temperature measurement settings are found in Temperature Setup menu for each measurement. Temperature Unit ºC or ºF selection Temperature Input and Type Ch-1 ATC, Ch-2 ATC, Ch-3 ATC, Ch-4 ATC, MTC Ch-1* ATC only or All ATC allows all ATCs to be Temperature Cal calibrated at once * This number will change to match the setup menu channel. Temperature probes can be set up in the measurement mode of the sample you are measuring. Ch-1 ATC allows the temperature probe to be used in more than one channel for temperature-compensated measurements, such as when a pH electrode and a 2-cell conductivity cell are in the same measurement sample. Temperature is a critical pH and conductivity measurement as the measurement values require a temperature correction to determine the reference value, often 25 ºC in the America and 20 ºC in Europe. Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module...
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  • Page 13: Ph, Rmv And Orp Calibration

    2. Follow screen prompts for calibration. Use fresh buffers, rinse electrode with DI water before each reading, blot with a lint free tissue. 3. USA and DIN buffer sets are automatically recognized based off the selection in the setup menu, and the meter allows custom buffers to be used for up to 6 calibration points. Custom buffer option prompts for the nominal values and stores data. 4. During calibration, points can be edited or retested prior to acceptance. 5. Information for the last calibration can be displayed on the main measurement screen. Refer to this reference guide, Chapter 2 Module Overview, Display Information section for more information. RmV Calibration One standard can be used for relative mV calibration. Always use fresh standard. Prepare the electrode according to the instructions in the electrode user guide. 1. Press f1 (Cal) to enter calibration mode. 2. Follow the screen prompts for calibration. 3. The calibration can be saved or printed when complete. 4. Information for the last calibration can be displayed on the main measurement screen. Refer to this reference guide, Chapter 2 Module Overview, Display Information section for more information. Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module...

  • Page 14: Orp Calibration

    ORP Calibration The mV reading of an ORP standard is dependent on the ORP electrode reference system, reference material, filling solution and ORP standard temperature. An ORP electrode can be automatically calibrated to read 420 mV relative to the Standard Hydrogen Electrode (E ) at 25 °C when the Orion ORP standard (cat. no. 967901 or 96791), Orion ORP electrode (cat. no. 9678BNWP, 9778BNWP, 9180BNMD or 9179BNMD) and Orion 4M KCl filling solution (cat. no. 900011) or 4M KCl gel (in electrode with cat. no. 9179BNMD) are used. The raw mV reading of an electrode with the same conditions is 220 mV at 25 °C. One standard can be used for ORP calibration. Always use fresh standard. Prepare the electrode according to the instructions in the electrode user guide. 1. Press f1 (Cal) to enter calibration mode. 2. Follow the screen prompts for calibration. 3. The calibration can be saved or printed when complete. 4. Information for the last calibration can be displayed on the main measurement screen. Refer to this reference guide, Chapter 2 Module Overview, Display Information section for more information. Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module...

  • Page 15: Ise Calibration

    4. The options after Cal Done is pressed are Print (f1), Cal Edit (f2) or Slope Edit (f3). 5. The Calibration report table is displayed with the concentration, mV reading, and Temperature and average slope which is updated with each successive point measured. 6. From this screen the navigation options are Print (f1), Cal Edit (f2) or Slope Edit (f3). Print exports data to printer or computer, Cal edit allows concentration to be corrected prior to the completion of the calibration by Delete (f1) or ReMeasure (f3). 7. Pressing the Slope Edit changes the screen title to Single Point Cal – Slope Edit. This allows the current slope line to be updated with the present electrode performance. 8. Press Cal Done (f1) when completed, press view graph, save the calibration in CalLog. Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module...
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  • Page 17: Temperature Calibration

    Chapter 6 Temperature Calibration The ATC temperature display has a relative accuracy of ±0.1 °C. ATC probes (both separate and those built into electrodes) have varying temperature accuracies, usually ±0.5 °C to ±2 °C. Use this function only if necessary. Since the temperature offset calculated during the calibration is applied to all future temperature measurements, recalibrate if a different ATC probe is used. For temperature calibration, the probe needs to be connected to the meter and the calibration solution should have a known, stable temperature. It is recommended that two NIST-traceable thermometers be used to measure and verify solution temperature. 1. Press f3 (setup) key. 2. Use the arrow keys to highlight the channel that the temperature probe is connected to. Press f3 (select). 3. Use the arrow keys to highlight Temperature and press f3 (select). 4. Use the arrow keys to highlight the appropriate Temperature Cal option and press f3 (select). 5. Follow the onscreen prompts. Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module...
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  • Page 19: Measurement

    3. If the meter is in AutoRead mode (meter default) press measure (esc) (1) key. If the meter is in continuous or timed read mode, the meter will immediately start taking readings. 4. If in AutoRead mode and datalog is enabled (default), the reading will automatically be stored when the “AR” appears. If in continuous read mode and datalog is enabled, press log/print (0) key to store into the meter’s memory. Note: To enable or disable datalogging, press setup and select Inst. Settings. ISE Measurement 1. Prepare the sample as instructed in the electrode user guide. 2. Follow directions carefully on ISA and other sample additions. 3. Rinse the probe(s) and place into the sample. If the meter is in AutoRead mode (meter default) press measure (esc) (1) key. If the meter is in continuous or timed read mode, the meter will immediately start taking readings. 4. If in AutoRead mode and datalog is enabled (default), the reading will automatically be stored when the “AR” appears. If in continuous read mode and datalog is enabled, press log/print (0) key to store into the meter’s memory. Note: To enable or disable datalogging, press setup and select Inst. Settings. Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module...
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  • Page 21: Methods

    Chapter 8 Methods The meter stores up to ten methods per channel with the last calibration data stored for each method. Use the channel setup to access and modify methods. 1. Press f3 (setup) key. 2. Use the arrow keys to highlight the appropriate channel. Press f3 (select). 3. Use the arrow keys to highlight Method and press f3 (select). 4. The methods can be loaded, copied, saved and edited by following the onscreen prompts. Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module...
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  • Page 23: Incremental Techniques

    Known Addition and Subtraction The Orion VERSA STAR meter has preset methods for single known addition, single known subtraction, double known addition and double known subtraction. • When performing incremental techniques, the sample concentration must be known within an order of magnitude so the increment may be correctly chosen and the sample and standard must be volumetrically determined. • It is very important that any complexing agent is present in excess (at least 50 to 100 times) or not at all. The ratio of free to complexed ions must remain constant over the addition. An indication that a complexing agent is present, but not in great enough quantity to maintain a constant free ion to complexed ion ratio, is an abnormally high or low slope. If this is a suspected problem, add an excess amount of complexing or decomplexing agent to the original solution in the beaker. • Electrode interferences should be at a minimum, since the effect of the interference might change as the concentration of the ion of interest changes. This situation may also result in high or low electrode slopes. Consult the electrode user guide for information on reducing or eliminating some electrode interferences. • Known addition is a useful method for measuring dilute samples, occasional samples or samples that contain an excess of complexing agent. Known addition can also be used to verify the results of a direct measurement. • Known subtraction is useful when measuring ions for which stable standards do not exist. Known subtraction can also be used as a quick version of a titration. When performing a known subtraction, it is necessary to know the stoichiometric ratio between the standard and sample. • Double incremental techniques calculate the electrode slope and sample concentration simultaneously. The electrode slope is determined directly in the sample, which results in greater accuracy for samples with complex matrices and greater analysis speed. Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module...

  • Page 24: Single Known Addition

    • Ensure that the electrode, ATC probe and reference electrode (if applicable) are connected to the meter inputs that correspond with the selected channel. Double Known Addition For most double known addition analysis, it is recommended that: • The sample volume should be 100 mL. • The concentration of the standard added should be 100 times the expected sample concentration. • Calculate the volume of standard required to halve the initial sample concentration and divide by six. Five sixths of the volume will be added in the first addition and the remainder in the second. If available, consult a printout from a previous analysis of a similar sample. Note: the proposed values for constant mV will be smaller than those recommended for known addition. This is an effort to minimize me chance of over "subtracting" the sample (overshooting the endpoint). Refer to the appropriate electrode user guide for instructions for preparing the electrode, required solutions (ionic strength adjusters, standards, etc) and any special requirements. Ensure that the electrode, ATC probe and reference electrode (if applicable) are connected to the meter inputs that correspond with the selected channel. Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module...

  • Page 25: Single Known Subtraction

    Ensure that the electrode, ATC probe and reference electrode (if applicable) are connected to the meter inputs that correspond with the selected channel. Double Known Subtraction Ensure that the subtraction ability of the chosen standard concentration and volume added does not exceed the concentration of sample. For most double known subtraction analysis, it is recommended that: • The sample volume should be 100 mL. • The concentration of the standard added should be 50 times the expected sample concentration. • Calculate the volume of standard required to halve the initial sample concentration and divide by six. Five sixths of the volume will be added in the first addition and the remainder in the second. If available, consult a printout from a previous analysis of a similar sample. Note: the proposed values for constant mV will be smaller than those recommended for known addition. This is an effort to minimize me chance of over "subtracting" the sample (overshooting the endpoint). Refer to the appropriate electrode user guide for instructions for preparing the electrode, required solutions (ionic strength adjusters, standards, etc) and any special requirements. Ensure that the electrode, ATC probe and reference electrode (if applicable) are connected to the meter inputs that correspond with the selected channel. Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module...

  • Page 26: Analyte Addition And Subtraction

    The concentration of the analyte in interest in the unknown is then calculated. • Isopotential value needs to be known and entered. • Blank correction is not applied. • The slope value at 25°C needs to be known and entered. To Access Incremental Techniques 1. Set the measurement mode to ISE. 2. Press f3 (setup) key. 3. Use the arrow keys to highlight the ISE channel. Press f3 (select). 4. Use the arrow keys to highlight Incremental Techniques and press f3 (select). 5. Follow the onscreen prompts. Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module...

  • Page 27: Data Storage And Review

    For more information on these settings, refer to the Orion VERSA STAR Reference Guide, Chapter 3 Instrument Settings to turn on Data Log to enable data storage. Datalog Review This option allows for stored data to be: • reviewed • printed individually, in a group or all • deleted • analyzed with general statistical information The datalog will reflect a screen capture of all the information at the time of datalogging. To review datalog information • Press log view (9) key • Press f3 (select) key • Follow prompts to review information Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module...

  • Page 28: Calibration Review

    Calibration Review This feature allows for review and printing of each of the last 10 calibrations for each parameter of the meter. The information will match the information recorded for that calibration. To review calibration information 1. Press log view (9) key. 2. Press right arrow (6) key to select CalLog information and down arrow (8) to highlight the desired parameter. 3. Press f3 (select) key and follow the prompts. Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module...

  • Page 29: Customer Services

    Recommended Actions Measurement is outside the allowable measurement range. Check electrode Measurement is connection and settings in the setup menu. Clean the electrode according to the flashing 9999 and over electrode user guide and recalibrate the electrode with new buffers or standards. range or under range. If the error continues, perform the meter self test in the diagnostics menu. The measurement The meter is in AutoRead mode. (AR icon appears solid on the display.) Press freezes and will measure (esc)/1 key to take a new reading or go to setup to change the read type. not change. Assistance After troubleshooting all components of your measurement system, contact Technical Support. Within the United States call 1.800.225.1480 and outside the United States call 978.232.6000 or fax 978.232.6031. In Europe, the Middle East and Africa, contact your local authorized dealer. For the most current contact information, or the latest application and technical resources for Thermo Scientific Orion products, visit www.thermoscientific.com/water. Warranty and Registration To register your new meter and for the most current warranty information, visit www.thermoscientific.com/water. WEEE Compliance This product is required to comply with the European Union’s Waste Electrical & Electronic Equipment (WEEE) Directive 2002/96/EC. It is marked with the symbol above. Thermo Fisher Scientific has contracted with one or more recycling/disposal companies in each EU Member State and this product should be disposed of or recycled through them. Further information on compliance with these directives, the recyclers in your country, and information on Thermo Scientific Orion products that may assist the detection of substances subject to the RoHS Directive are available at www.thermoscientific.com. Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module...

  • Page 30: Declaration Of Conformity

    Measurement, control and laboratory Orion Star A-series meters are EMC Class A Conforms with the following directives and standards: EN61326-1:2006 Electromagnetic Compatibility (EMC Directive) Electrical equipment for measurement, control and laboratory use - EMC requirements EN61010-1:2001 Safety Standards UL61010-1:2004 Safety requirements for electrical equipment for measurement, CAN/CSA C22 .2 No . 61010-1-04 control and laboratory use - general requirements Cheow Kwang Chan Place and Date of Issue: QA/Regulatory Manager 12 December, 2011 Singapore Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module...

  • Page 31: Ph/Ise/Temperature Module Specifications

    Up to 4 significant digits Relative Accuracy ±0.2 mV or ±0.05 % of reading whichever is greater Units ppm, M, mg/L, %, ppb, none Calibration Points Up to 6 Calibration Editing Adjustable ISO Point Timed end point, linear point to point, non-linear Calibration Features selectable auto-blank, low concentration range stability Single known addition & subtraction, Incremental Techniques double known addition & subtraction, single analyte addition & subtraction Advanced Features Displays electrode type, temperature compensation Temperature Range -5 to 105 °C, 23 to 221 ºF Resolution Relative Accuracy ±0.1 Offset Calibration 1 point Inputs pH/ISE: BNC, reference pin ATC: 8-pin mini-DIN NOTE: We reserve the right to make improvements. Specifications subject to change without notice. Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module...

  • Page 32: Ordering Information

    - Electrode arm with redesigned holder - Universal power adapter VSTAR30 Orion VERSA STAR RDO/Dissolved Oxygen Benchtop Meter Set - Orion VERSA STAR meter - Orion VERSA STAR dissolved oxygen/temperature module - Electrode arm with redesigned holder - Universal power adapter VSTAR32 Orion VERSA STAR RDO/Dissolved Oxygen Benchtop Meter Kit - Orion VERSA STAR meter - Orion VERSA STAR dissolved oxygen/temperature module - 083005MD Orion polarographic DO probe - 080017 calibration sleeve for DO probe - 080513 DO probe maintenance kit - 970802 BOD funnel/stirrer - 080360 BOD adapter - Electrode arm with redesigned holder - Universal power adapter Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module...
  • Page 33 - Two electrode arms with redesigned holders - Universal power adapter VSTAR50 Orion VERSA STAR pH/Conductivity Benchtop Meter Set - Orion VERSA STAR meter - Orion VERSA STAR pH/temperature module - Orion VERSA STAR conductivity/temperature module - Electrode arm with redesigned holder - Universal power adapter VSTAR52 Orion VERSA STAR pH/Conductivity Benchtop Meter Kit - Orion VERSA STAR meter with pH/temperature module - Orion VERSA STAR conductivity/temperature module - 8157BNUMD Orion ROSS Ultra Triode pH/ATC probe - 013005MD Orion DuraProbe conductivity cell, K=0.475 - 810199 ROSS solution kit (475 mL each of pH 4, 7 and 10 buffers; storage solution; cleaning solution; and pH electrode storage bottle) - 011007 conductivity standard, 1413 µS, 5x60 mL - Electrode arm with redesigned holder - Universal power adapter Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module...
  • Page 34 VSTAR90 Orion VERSA STAR pH/ISE/Conductivity/RDO/Dissolved Oxygen Benchtop Multiparameter Meter Set - Orion VERSA STAR meter - Orion VERSA STAR pH/ISE/temperature module - Orion VERSA STAR conductivity/temperature module - Orion VERSA STAR dissolved oxygen/temperature module - Electrode arm with redesigned holder - Universal power adapter VSTAR91 Orion VERSA STAR pH/ISE/Conductivity/RDO/Dissolved Oxygen Benchtop Multiparameter Meter Set - Orion VERSA STAR meter - Two Orion VERSA STAR pH/ISE/temperature modules - Orion VERSA STAR conductivity/temperature module - Orion VERSA STAR dissolved oxygen/temperature module - Two 096019 Orion Star stirrer probes - Two electrode arms with redesigned holders - Universal power adapter Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module...
  • Page 35 - 810199 ROSS solution kit (475 mL each of pH 4, 7 and 10 buffers; storage solution; cleaning solution; and pH electrode storage bottle) - 011007 conductivity standard, 1413 µS, 5x60 mL - Electrode arm with redesigned holder - Universal power adapter VSTAR93 Orion VERSA STAR pH/ISE/Conductivity/RDO/Dissolved Oxygen Ultimate Benchtop Multiparameter Meter Set - Orion VERSA STAR meter - Orion VERSA STAR pH/ISE/temperature module - Orion VERSA STAR conductivity/temperature module - Orion VERSA STAR dissolved oxygen/temperature module - 8157BNUMD Orion ROSS Ultra Triode pH/ATC probe - 013005MD Orion DuraProbe conductivity cell, K=0.475 - 083005MD Orion polarographic DO probe - Two 096019 Orion Star stirrer probes - 080017 calibration sleeve for DO probe - 080513 DO probe maintenance kit - 970802 BOD funnel/stirrer - 080360 BOD adapter - 810199 ROSS solution kit (475 mL each of pH 4, 7 and 10 buffers; storage solution; cleaning solution; and pH electrode storage bottle) - 011007 conductivity standard, 1413 µS, 5x60 mL - Two electrode arms with redesigned holders - Universal power adapter Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module...
  • Page 36 1010001 Orion conductivity calibration resistor kit 9512HPBNWP Orion high-performance ammonia ISE 9512BNWP Orion standard ammonia ISE 951007 Ammonia standard, 1000 ppm, 475 mL 951210 Low-level ammonia ISA 951211 Ammonia ISA, 475 mL 951209 Fill solution for Orion high-performance ammonia ISE, 60 mL 951202 Fill solution for standard ammonia ISE , 60 mL 951213 Ammonia ISE storage solution 9609BNWP Orion fluoride combination ISE 040906 Fluoride standard, 1 ppm with TISAB II, 475 mL 040907 Fluoride standard, 2 ppm with TISAB II, 475 mL 040908 Fluoride standard, 10 ppm with TISAB II, 475 mL 940909 TISAB II, 1 gallon 8611BNWP Orion ROSS sodium combination ISE Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module...

  • Page 37: Advanced Features

    Testing a pH Electrode for Automatic Buffer Recognition To verify that the raw mV reading of the pH electrode in use is ± 30 mV from the theoretical mV reading of the buffer, and therefore verify that the pH electrode in use is capable of performing automatic buffer recognition, perform the following procedure. 1. Set the measurement mode to mV. Prepare the pH electrode according to the instructions in the pH electrode user guide. 2. Rinse the pH electrode with distilled water, blot it dry with a lint-free tissue and place the pH electrode into a pH 4.01 buffer at approximately 25 °C. 3. Wait for the measurement to stabilize and record the mV value of the pH 4.01 buffer when the meter indicates that the measurement is stable. The read type selected in the setup menu will determine how the mV measurements are displayed by the meter. 4. Remove the pH electrode from the pH 4.01 buffer. 5. Rinse the pH electrode with distilled water, blot it dry with a lint-free tissue and place the pH electrode into a pH 7.00 buffer at approximately 25 °C. 6. Wait for the measurement to stabilize and record the mV value of the pH 7.00 buffer when the meter indicates that the measurement is stable. 7. The mV reading of the pH electrode in pH 4.01 buffer should be in the range of +207 to +147 mV and the mV reading of the pH electrode in pH 7.00 buffer should be in the range of -30 to +30 mV. If the mV readings of the pH electrode are in the correct ranges, the pH electrode is capable of performing automatic buffer recognition. If the mV readings of the pH electrode are not in the correct ranges, the values of the pH buffers will need to be manually entered during a pH calibration. Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module...

  • Page 38: Ph Electrode Condition Icon

    Electrode Condition Icon The electrode condition icon indicates the performance of the pH electrode, based on the last saved calibration and electrode measurement stability. Icon pH Electrode Definition of Icon Electrode condition is good and the electrode slope is 90 to 110 %. Electrode condition is fair and the electrode slope is 80 to 120 %. Electrode condition is bad and the electrode slope is less than 80 % or greater than 120 %. Consult the electrode user guide for instructions on how to clean, condition and troubleshoot the electrode. Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module...

  • Page 39: Multipoint Calibration Curve Feature

    Multipoint Calibration Curve Feature The Orion VERSA STAR Meter with pH/ISE/Temperature module systems utilize a point-to-point scheme for multipoint calibrations in the pH and ISE modes. In this technique, straight line segments are drawn between each successive pair of calibration points. Separate slope and E values are calculated using the adjacent pair of data points. For example, in a five point calibration, four slopes and five E values are calculated to correspond to the four line segments that connect the data. pH or concentration values for samples are calculated according to the segment that their potential values fall into. Those above or below the range of calibration are calculated by extrapolation from the top or bottom most segment. The displayed slope is an average slope of the separate segments. The slope may be outside the range normally expected for an electrode. For example, if the slope of one segment is low, then the average will be lower than theoretical, even though the response may be Nernstian over most of the range. The figure below is an example of a typical calibration curve using this method. Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module...

  • Page 40: Automatic Blank (Non-Linear) Correction Feature

    E1 = Eo + S * log (C1 + b) E2 = Eo + S x log (C2 + b) E3 = Eo + S x log (C3 + b) The meter evaluates the relationships between the three potentials E1, E2 and E3 and the three concentrations C1, C2 and C3. If the relationships indicate that blank correction is desired, a blank will automatically be calculated and the non-linearity will be corrected for in a Nernstian manner. If the appropriate conditions are not met, the blank is set at zero and each segment of the multipoint calibration is treated independently. Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module...

  • Page 41: Determining An Isopotential Value

    2. Potential differences between points are significant. For example, E3 - E1 > 10 mV 3. The blank correction algorithm converges at reasonable blank and slope values. Conditions in steps 1 and 2 prevent failure to converge in most situations. However, the slope will attain any value necessary for convergence to a calculated blank value of 3 x C3. When blank correction is implemented, the slope value for the electrode may be outside the range of values normally considered acceptable during an ordinary calibration. For best results, calibration standards should be close in range to the expected sample concentrations and should bracket the expected sample concentration. When conditions 1, 2 and 3 are not met, the calibration data is handled by the multipoint calibration method described earlier. In calibrations with more than three points, a combination of methods is utilized. Automatic blank correction is used if the lowest three points satisfy the criteria and multipoint calibration is used for the other points. The slope displayed after calibration is an average of the slope calculated in the automatic blank correction algorithm and the slope(s) for each additional segment. Determining an Isopotential Value 1. Prepare several standards with concentration ranges over the measuring range of the electrode. 2. Measure the millivolt value of the standards at room temperature, about 20 °C to 25 °C. 3. Measure the millivolt value of each standard at 75 °C. 4. Measure the millivolt value of each standard at 10 °C. 5. On semi-logarithmic graph paper, plot the concentration values on the log axis versus the millivolt values on the linear axis. 6. The lines will intersect at the isopotential value. Read the concentration from the graph for this point from the log axis. This is the isopotential value. Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module...
  • Page 42 Notes Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module...
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  • Page 44 Water Analysis Instruments North America 9 0 0 1 : 2 0 0 8 166 Cummings Center Beverly, MA 01915 USA Toll Free: 1-800-225-1480 Tel: 1-978-232-6000 info.water@thermo.com Netherlands Tel: (31) 033-2463887 info.water.uk@thermo.com India Tel: (91) 22-4157-8800 wai.asia@thermofisher.com Japan Tel: (81) 045-453-9175 wai.asia@thermofisher.com China Tel: (86) 21-68654588...

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