Figure 23. Define Scan Dialog Box; Figure 24. Activation Type List In The Define Scan Dialog Box - Thermo Scientific ETD Getting Started Manual

5
Performing an ETD Infusion Experiment
Obtaining an ETD Spectrum for Angiotensin I
36 MS/ETD System Getting Started Guide
4. In the Tune Plus window, click the Define Scan (
dialog box (Figure 23).

Figure 23. Define Scan dialog box

5. Under MSn Settings, in the n = 2 row, enter the Parent Mass (m/z) of the 3+ charge state
of Angiotensin I.
The molecular weight of Angiotensin I (acetate hydrate) is 1296 Da. The mass-to-charge
ratio of the parent ion [M + 3H]
6. In the Act. Type list, select ETD

Figure 24. Activation Type list in the Define Scan dialog box

3+
is 433.0.
(Figure 24).
Activation Type = ETD
) button to open this
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