Optimizing The Reagent Ion Reaction Time; Figure 26. Tune Dialog Box For Etd - Thermo Scientific ETD Getting Started Manual

5
Performing an ETD Infusion Experiment

Optimizing the Reagent Ion Reaction Time

Optimizing the Reagent Ion Reaction Time
38 MS/ETD System Getting Started Guide
The default reagent ion reaction time of 100 ms is appropriate for most analyses. In some
cases obtaining an optimized reagent ion reaction time is helpful for a specific analyte. The
procedures in this section assume that the system generates the reagent ions as described in
"Turning On the Reagent Ion Source"
 To optimize the reagent ion reaction time
1. Turn on ETD activation for the analyte (Angiotensin I, in this case).
2. Open the Tune Plus window; see the instructions on
3. Click the Define Scan (
page 36).
4. Under MSn Settings, in the Parent Mass (m/z) box, enter the mass for the analyte.
5. Under MSn Settings, in the Act. Type list, select ETD.
6. In the Tune Plus window, click the Tune (
The Tune dialog box has two additional tabs for tuning ETD

Figure 26. Tune dialog box for ETD

on page 28.
page 13.
) button to open this dialog box
) button to open this dialog box.
(Figure 23 on
(Figure 26).
Thermo Scientific