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3
Tuning
Tuning the Reagent Ion Optics
Semi-Automatically Tuning the Reagent Ion Optics
22
MS/ETD System Getting Started Guide
Use the semi-automatic tuning method to fine-tune the lens parameters to a range of settings
and in step increments.
To semi-automatically tune the reagent ion optics
1. Open the Tune Plus window; see the instructions on
2. Click the Display Graph View (
3. Turn on the Reagent Ion Source as follows:
a. Choose Setup > Reagent Ion Source
b. Select the Reagent Ion Source On check box.
c. Select the Filament On check box.
Note
If the reagent vials are not at their target temperature, a message appears:
Reagent Vial NOT At Temperature! Please wait ...
The System LED on the ETD module flashes green to indicate that the reagent
vial heaters are turned on but not at their target temperatures (the other heaters
are at their target temperatures).
When the reagent vial heaters reach their target temperatures, the System LED
illuminates solid green.
When the reagent vials reach their target temperature, voltage is applied to the
ETD module ion optics, and when the check box is selected, the filament turns
on and the Filament LED illuminates solid green.
4. In the Reagent Ion Source dialog box, select the View Reagent Ion Spectra check box.
5. In the Tune Plus window, click the Tune (
on
page
18).
page
13.
) button.
(Figure 8
on
page
16).
) button to open this dialog box
(Figure 10
Thermo Scientific
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Troubleshooting
