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1
Thermo Scientific ETD manual available for free PDF download: Getting Started Manual
Thermo Scientific ETD Getting Started Manual (74 pages)
For the LTQ XL and LTQ Velos MS Detectors (compatible with LTQ Orbitrap XL and LTQ Orbitrap Velos)
Brand:
Thermo Scientific
| Category:
Control Unit
| Size: 4 MB
Table of Contents
Fcc Compliance Statement
4
Table of Contents
9
Preface
13
Related Documentation
13
Safety and Special Notices
14
Contacting Us
15
Chapter 1 Introduction
17
Electron Transfer Dissociation
18
Types of Experiments
18
Figure 1. LTQ XL/ETD System New Method Page
19
ETD Experiment Types
20
LTQ XL and LTQ Velos Experiment Types
20
Tuning and Calibration
21
Diagnostics
21
Chapter 2 Setting up the Xcalibur Instrument Configuration
23
Adding the MS Detector to the Xcalibur Instrument Configuration
23
Figure 2. Thermo Foundation Instrument Configuration Window
24
Devices
25
Figure 3. Thermo Foundation Instrument Configuration Window with Configured
25
Specifying the Reagent Ion Source for the MS Detector
26
Figure 4. LTQ XL Configuration Dialog Box
26
Configuration
27
Figure 5. LTQ Velos Configuration Dialog Box with the Reagent Ion Source
27
Figure 6. LTQ Velos Configuration Dialog Box with the Low Vial Temperature Option
27
Tuning
29
Opening the Tune Plus Window
29
Chapter 3 Tuning
29
Tuning the Reagent Ion Optics
30
Figure 7. Tune Plus Window
30
Automatic Tuning of the Reagent Ion Optics
31
Figure 8. Reagent Ion Source Dialog Box
32
Figure 9. Spectrum Displayed in Tune Plus
33
Figure 10. Automatic Page in the Tune Dialog Box
34
Manually Tuning the Reagent Ion Source
35
Figure 11. Reagent Ion Optics Dialog Box
36
Figure 12. Manual Page in the Tune Dialog Box
36
Figure 13. Graph View of the Reagent Ion Source Tuning in the Tune Plus Window
37
Semi-Automatically Tuning the Reagent Ion Optics
38
Figure 14. Semi-Automatic Page in the Tune Dialog Box
39
Viewing the Reagent Ion Optics Settings
40
Saving the ETD Tune Method
40
Figure 15. Reagent Ion Optics Dialog Box
40
Chapter 4 Daily Operation
41
Before Operating the LTQ XL/ETD or LTQ Velos/Etd System
41
Checking the System Mode
41
Checking the Vacuum Pressure
42
Figure 16. Ion Trap Page
42
Checking the Gas Supplies
43
Figure 17. Reagent Page
43
Turning on the Reagent Ion Source
44
Figure 18. Reagent Ion Source Dialog Box
44
Figure 19. Fluoranthene Radical Anion Mass Spectrum
46
After Operating the System
47
Placing the System in Standby Mode
47
Figure 20. Reagent Ion Source Dialog Box
47
Purging the Oil in the Fore Pump
48
Chapter 5 Performing an ETD Infusion Experiment
49
Viewing the Injection Reagent Settings
49
Figure 21. Ion Trap Page in the Injection Control Dialog Box
49
Figure 22. Reagent Page in the Injection Control Dialog Box
50
Troubleshooting an AGC Target Error
51
Obtaining an ETD Spectrum for Angiotensin I
51
Figure 23. Define Scan Dialog Box
52
Figure 24. Activation Type List in the Define Scan Dialog Box
52
Figure 25. ETD MS/MS Spectrum of Angiotensin I
53
Optimizing the Reagent Ion Reaction Time
54
Figure 26. Tune Dialog Box for ETD
54
Figure 27. Reagent Ion Reaction Time Page in the Tune Dialog Box
55
Chapter 6 Running an Xcalibur Experiment by Using ETD
57
Nth Order Double Play (ETD) Experiment
58
Figure 28. Xcalibur Roadmap View of the Home Page
58
Figure 29. Instrument Setup Window for Thermo EASY-Nlc
59
Figure 30. New Method Page for the LTQ XL/ETD System
60
Figure 31. Nth Order Double Play Dialog Box
60
Figure 32. Nth Order Double Play with ETD Template
61
Figure 33. Data Dependent Settings Dialog Box
62
Figure 34. Activation Parameters
62
Data Dependent NL MS Experiment
63
Figure 35. Data Dependent NL MS3 Dialog Box
63
Figure 36. Neutral Loss Masses Table Example
64
Figure 37. Data Dependent NL MS3 Template
65
Chapter 7 Troubleshooting
67
Appendix A Fluoranthene
69
Figure 38. ETD Reagent (Fluoranthene Radical Anion) Generation from Fluoranthene
69
Appendix B Angiotensin I Solutions
71
Index
73
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