Thermo Scientific ETD Getting Started Manual page 66

6
Running an Xcalibur Experiment by Using ETD
3
Data Dependent NL MS Experiment
50 MS/ETD System Getting Started Guide
12. In the Isolation Width (m/z) box, enter a value between 2 and 3.
The Normalized Collision Energy and Activation Q parameters are unavailable because
they are not applicable to ETD.
13. In the Activation Time (ms) box, leave the default value or enter a different value as
described in "Optimizing the Reagent Ion Reaction Time"
14. In the Tune Plus window, click the Save
15. Browse to the :\Xcalibur\methods folder, and specify a file name.
16. Click Save.
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