Figure 10. Automatic Page In The Tune Dialog Box - Thermo Scientific ETD Getting Started Manual

For the ltq xl and ltq velos ms detectors (compatible with ltq orbitrap xl and ltq orbitrap velos)

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Tuning

Tuning the Reagent Ion Optics

MS/ETD System Getting Started Guide

6. In the Tune Plus window, click the Tune (

(Figure

10).

Figure 10. Automatic page in the Tune dialog box

7. Click Start.

The system begins automatically tuning the ion optics of the reagent ion source. The

Status area displays the message "Optimization Complete" after completing automatic

tuning. This message also states the percentage change in the reagent ion signal

(m/z = 202) intensity relative to the prior value. A typical reagent signal intensity is about

5E6 to 1E7 in profile mode when the system has been cleaned and the ion volume is new.

8. Repeat Automatic Tune if the percentage change is greater than 20%.

Note This is an iterative process. At some point there will be no more improvements in

signal intensity.

) button to open this dialog box

Nee

Thermo Scientific

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