Figure 27. Reagent Ion Reaction Time Page In The Tune Dialog Box - Thermo Scientific ETD Getting Started Manual

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7. Click the Reagent Ion Reaction Time tab

Figure 27. Reagent Ion Reaction Time page in the Tune dialog box

8. Click Start.
Tune Plus generates a graph showing the intensity of the m/z of interest versus reaction
time. The Status area of the window shows a reagent ion reaction time after completing
the tune process.
A message prompts you to accept the optimized value. If you accept the optimized value,
the reagent ion reaction time parameter sets to this optimized value in the Define Scan
dialog box. Otherwise, it returns to its previous value.
The reagent ion reaction time is now optimized based on total ion current (TIC).
To optimize on a particular product ion, in the Tune dialog box under What to Optimize
On (Figure 27), select the Product Ion Mass (m/z) option, enter the m/z of the selected
product ion, and then repeat step 7.
5
Performing an ETD Infusion Experiment
Optimizing the Reagent Ion Reaction Time
(Figure 27).
MS/ETD System Getting Started Guide 39