Procedure; Quality Control; Results; Limitations Of The Procedure - Siemens ADVIA 2120 Operator's Manual

Procedure

To run samples on your ADVIA 2120/2120i Hematology system, refer to the
Daily Routine.

Quality Control

It is recommended that the system be controlled using ADVIA TESTpoint
Hematology Controls. Please refer to page 9-5 for product descriptions. These
controls are intended to be integrated into a clinical laboratory's own quality
control program and procedures.
Control materials should be assayed:
•
At the beginning of each shift or at some other interval chosen by the
laboratory.
•
After a reagent lot number change.
•
After replacement of any part or component of the analytical module that
may affect analytical performance.
As an option, laboratories may run a retained patient sample periodically to
monitor performance trends.

Results

The system automatically performs all calculations necessary for obtaining final
results.
Through the use of complex flagging algorithms, laboratory personnel are alerted
to suspected abnormal conditions. These conditions are indicated by the
appropriate flag (such as * , +, and/or color highlighting). Whenever flags occur,
the user should review the results and take appropriate action.

Limitations of the Procedure

The limitations of the procedure are as listed:
1. Certain abnormal specimens such as those from hemodialysis patients,
patient specimens with nucleated RBCs (particularly neonates), as well as
specimens exhibiting platelet clumping or incomplete lysis of RBCs, may
interfere with white cell differential.
2. Circulating micromegakaryocytes may be counted as white blood cells.
3. Incomplete RBC lysis in the Peroxidase channel may be observed in
specimens with elevated serum urea nitrogen (BUN > 75 mg/dL).
4. Interference with the performance of the Peroxidase channel may be noted in
specimens obtained from patients with inherited or acquired deficiency of the
myeloperoxidase enzyme. Such deficiency has been observed in
approximately 0.5% of laboratory specimens.
Regulatory Information 9-51