Sample Handling; Materials Required But Not Provided - Siemens ADVIA 2120 Operator's Manual

Sample Handling

Sample Collection
Collect whole blood in an evacuated blood collection tube containing EDTA as
an anticoagulant. Blood samples should be refrigerated at a temperature from 2°C
to 8°C, if they will not be analyzed within eight (8) hours of phlebotomy. If a
specimen has been refrigerated, allow it to equilibrate slowly to room
temperature (15°C to 30°C) before analysis.
Sample Dilution
Whole-blood specimens with values exceeding the analytical range may be
diluted with autologous plasma or isotonic saline and reassayed.
Sample Stability
The effect of the aging of blood was studied over a 72 hour period on the ADVIA
120 Hematology System.
Two whole-blood specimens drawn from 15 normal, apparently healthy donors
were assayed shortly after phlebotomy and then again at intervals of 8, 24, 36,
48, 56, and 72 hours.
One of the whole-blood specimens from each pair was stored at room
temperature while the corresponding specimen was stored at 2°C to 8°C in
capped blood collection tubes that contained EDTA as the anticoagulant.
The results indicate that WBC DIFF parameters are stable within 2 standard
deviations (within run precision) of the initial recovery for the specified time
interval.
Parameter
%NEUT
%LYMPH
%MONO
%EOS
%BASO
%LUC

Materials Required but not Provided

For the ADVIA 2120/2120i Hematology System, the other materials required to
perform the ADVIA 120 WBC DIFF method are the various control materials,
calibrators, ADVIA OPTIpoint, and other reagents or equipment specified in the
"Methods Introduction" section.
9-50
Room Temperature
Stability (hours)
36
36
72
8
72
72
Refrigerated Temperature
Stability (hours)
72
72
72
72
56
72
Regulatory Information