Rbc Volume / Hemoglobin (V / Hc) Cytogram - Siemens ADVIA 2120 Operator's Manual

1 Low-angle light scatter (2° to 3°)
2 High-angle light scatter (5° to 15°)
3 Mie map containing RBCs
4 Platelets detected in RBC method
Using the Mie theory of light scattering for homogeneous spheres, the low-angle
and high-angle light scatter signals for each cell are transformed into volume and
hemoglobin concentration values.
The RBC map shows the relationship between the light-scatter measurements and
the cell-by-cell characteristics of volume and hemoglobin concentration. The
map grid encompasses RBC volumes between 30 fL and 180 fL and hemoglobin
concentrations between 19 g/dL and 49 g/dL.
For a normal specimen, the RBC population appears in the center of the map.
However, since this map is nonlinear, visually evaluating red cell morphology
can be difficult. Use the V/HC cytogram, which provides a linear presentation of
cell volume and hemoglobin concentration.

RBC Volume / Hemoglobin (V / HC) Cytogram

The Volume/Hemoglobin Concentration (V/HC) cytogram is a linear version of
the RBC map that appears on the RBC cytogram. On the V/HC cytogram,
hemoglobin concentration is plotted along the x axis and cell volume is plotted
along the y axis. Only red blood cells appear on this cytogram.
8-44 Methods