Reticulocyte Method; Cytochemical Reactions; Advia 2120/2120I Autoretic; Measurement - Siemens ADVIA 2120 Operator's Manual

Reticulocyte Method

Cytochemical Reactions

The reticulocyte cytochemical reactions consist of two steps:
Step 1
Step 2

ADVIA 2120/2120i autoRETIC

NOTE:
autoRETIC reagent, please see Chapter 8.
The ADVIA 2120/2120i autoRETIC reagent contains a zwitterionic detergent
(surfactant) that isovolumetrically spheres the red cells. It also contains a cationic
dye, Oxazine 750, that stains cells according to their RNA content.

Measurement

A constant volume of the cell suspension from the retic reaction chamber passes
through the flowcell where the low-angle light scatter (2° to 3°), the high-angle
light scatter (5° to 15°), and the absorption signatures for each cell are measured.
ADVIA 2120/2120i SHEATH/RINSE encases the sample stream as the two
fluids pass through the flowcell.
The low-angle and high-angle light scatter signatures are proportional to cell size
and hemoglobin concentration. Light absorption is proportional to RNA content.
The stained reticulocytes will absorb more light than the mature RBCs.
The two light-scatter signals and the absorption signal are detected, electronically
amplified, and split into six signals.
One of the low-angle light scatter signals is amplified 28 times.
One of the high-angle light scatter signals is amplified 12 times.
One of the absorption signals is amplified 33 times.
After processing, this information is available:
RETIC Rate
RETIC Abs Flatness
intervals.
RETIC Abs
reticulocytes by their absorption values.
RETIC Volume
reticulocytes by their cell volume independent of hemoglobin concentration.
RETIC HC
reticulocytes by hemoglobin concentration independent of cell volume.
8-58
RBCs and platelets are isovolumetrically sphered using ADVIA
2120/2120i autoRETIC reagent.
Reticulocytes are differentially stained based on their RNA content.
For more detailed information on the contents of ADVIA 2120/2120i
histogram displays the arrival rate of cells in the retic channel.
histogram displays the mean absorption at 200-millisecond
histogram displays the overlaid distribution of mature RBCs and
histogram shows the overlaid distribution of mature RBCs and
histogram shows the overlaid distribution of mature RBCs and
Methods