Measurement; Measurement: Rbc Method; Measurement: Platelet Method - Siemens ADVIA 2120 Operator's Manual

Measurement

A constant volume of the cell suspension from the RBC reaction chamber passes
through the flowcell where the low-angle light scatter (2° to 3°) and high-angle
light scatter (5° to 15°) signatures of each cell are measured.
ADVIA 2120/2120i SHEATH/RINSE encases the sample stream as the two
fluids pass through the flowcell.
The two light-scatter signals are detected, electronically amplified, and split into
four signals:
•
A pair of low-angle light scatter (2° to 3°) and high-angle light scatter (5° to
15°) signals are used to analyze red blood cells.
•
A low-angle light scatter (2° to 3°) signal is amplified 30 times, and a high-
angle light scatter (5° to 15°) signal is amplified 12 times. These signals are
used to analyze platelets.

Measurement: RBC Method

A pair of low-angle light scatter (2° to 3°) and high-angle light scatter (5° to 15°)
signals are used to analyze red blood cells.
Using the Mie theory of light scattering for homogeneous spheres, the low-angle
light scatter measurement is converted into cell volume and the high-angle light
scatter measurement is converted into hemoglobin concentration.
The following histograms and cytograms are used for red blood cell analysis:
RBC Rate
RBC Volume
RBC HC histogram
concentration independent of cell volume.
RBC CH histogram
RBC Scatter
15°) along the x axis and the low-angle light scatter (2° to 3°) on the y axis.
RBC V/HC
data intended for evaluating red cell morphology.
RBC Matrix
regions on the RBC V/HC cytogram.

Measurement: Platelet Method

A low-angle light scatter (2° to 3°) signal is amplified 30 times, and a high-angle
light scatter (5° to 15°) signal is amplified 12 times. These "high-gain" signals
are used to analyze platelets.
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histogram shows the arrival rate of cells in the RBC/Platelet channel.
histogram shows the distribution of red cells by cell volume.
shows the distribution of red cells by hemoglobin
shows the distribution of red cells by hemoglobin content.
cytogram is formed by plotting the high-angle light scatter (5° to
cytogram is a presentation of the RBC volume and Hgb concentration
provides the cell counts and percentages for the corresponding nine
Methods