Materials Required But Not Provided; Procedure For Running Csf Samples - Siemens ADVIA 2120 Operator's Manual

Sample Stability
CSF patient samples are not stable and should be assayed as soon as possible
after collection. Siemens recommends that CSF patient samples be prepared with
CSF reagent within one hour after collection. Once prepared with CSF reagent,
the prepared sample is stable between 4 minutes and 4 hours when stored at
18ºC – 30ºC.
Prepared CSF patient samples and prepared ADVIA 120 TESTpoint CSF
controls contain 1% and 1.5% formaldehyde, respectively. These should be
disposed of according to local environmental regulations.
Sample and Control Preparation
Use the following procedure to prepare CSF samples or control products for
analysis on the ADVIA 2120/2120i Hematology System.
NOTE:
300 µL. Reserve sufficient sample volume for slide preparation or additional
analysis if necessary. Samples with high RBC counts may require dilution prior
to preparation with CSF Reagent. Phosphate buffered saline (PBS) is the
preferred diluent for these samples, although normal saline may be also used.
Samples diluted in normal saline should be prepared with CSF reagent
immediately after dilution and will require a longer incubation period than
undiluted samples or samples diluted in PBS.
1. Add 300 µL of thoroughly mixed CSF sample (patient or control) to 300 µL
of CSF Reagent in a labeled glass or plastic test tube.
2. Cap and mix the prepared sample by gently inverting it 5–10 times or
vortexing for 5 seconds. Care should be taken to avoid foaming.
3. Incubate it at room temperature for at least 4 minutes. Samples should be
assayed within 4 hours of preparation.
4. After preparing the sample, replace the reagent cap and return any quality
control materials to refrigerated storage.
5. After appropriate incubation, mix the sample again by inverting it 5–10 times
or vortexing it for 5 seconds immediately before aspirating on the ADVIA
2120/2120i Hematology System. Cells in prepared CSF samples will settle
quickly due to low viscosity.

Materials Required but not Provided

For the ADVIA 2120/2120i Hematology System, the other materials required to
perform the ADVIA 120 CSF method are the various control materials,
calibrators, ADVIA OPTIpoint, and other reagents or equipment specified in the
"Methods Introduction" section.

Procedure for Running CSF Samples

To run CSF samples on your ADVIA 120 Hematology System, perform the
following procedure:
9-36
The CSF Assay requires a CSF sample volume of
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