Reticulocyte Method Nomenclature; Retic Rate Histogram - Siemens ADVIA 2120 Operator's Manual

RETIC CH
reticulocytes by hemoglobin content (cellular hemoglobin).
RETIC Scatter Abs
light scatter and the high-gain absorption data. Sample-specific thresholds
separate the cell populations.
RETIC Scatter
scatter along the x axis, and the low-angle, low-gain light scatter on the y axis.
RETIC V / HC
hemoglobin concentration.

Reticulocyte Method Nomenclature

Mature (m), Reticulocytes (r), Gated (g)
The lowercase letters m, r, and g are commonly used in the reticulocyte
parameters to identify a specific population.
For example: MCVm, MCVr, and MCVg refer to the mean cell volume for the
mature RBC, reticulocyte, and gated populations, respectively.
"Gated" refers to the total population of RBCs containing both mature RBCs and
reticulocytes.
Negative versus Positive Cell Populations
Since reticulocytes have RNA and stain with ADVIA 2120/2120i autoRETIC
reagent, they are the positive cell population. Mature RBCs do not stain, and they
are the negative cell population.
Immature Reticulocyte Fraction
Immature Reticulocyte Fraction (IRF) is a descriptive term recommended in
NCCLS Document H44-A "Methods for Reticulocyte Counting: Flow Cytometry
and Supravital Dyes; Approved Guideline" to replace a previously-used term,
Reticulocyte Maturation Index (RMI).
Two IRF parameters are calculated: IRF-H and IRF-H+M.

RETIC Rate Histogram

The rate histogram data consists of 50 points, one taken every 200 milliseconds.
Each point represents the number of valid cells counted during the last 200
milliseconds.
Methods
histogram shows the overlaid distribution of mature RBCs and
cytogram is formed from the paired high-angle, low-gain
cytogram is formed by plotting the high-angle, low-gain light
cytogram provides a representation of the cell volume and
The RETIC Rate histogram
shows the uniformity of the
cell-counting rate.
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