Baso Cytogram: Histogram Analysis - Siemens ADVIA 2120 Operator's Manual

Polymorphonuclear Cluster
In normal samples, the PMN population contains eosinophil and neutrophil
nuclei.

BASO Cytogram: Histogram Analysis

If the cluster analysis does not agree with a normal archetype due to a low cell
count, or if there is only one identifiable cell population, the following cell
populations are identified using histogram analysis: Basophils, MN
(mononuclear cells), PMN (polymorphonuclear cells), Blasts, Noise, and Baso
Saturation area.
Methods
A Baso Noise threshold
B Blast threshold
C MN/PMN valley threshold
D Basophil threshold
E Baso Saturation threshold
1 Noise nuclei
2 Blast cell
3 Mononuclear WBCs (Monocyte
and Lymphocyte nuclei)
4 Basophils
5 not used
6 Saturation
7 Polymorphonuclear WBCs
(Neutrophil and Eosinophil nuclei)
BASO X histogram
8-7