Baso No Valley (B-Nv, Vb) - Siemens ADVIA 2120 Operator's Manual

System Related
1. Check baso reagents.
2. Check baso hydraulics.
3. Check the pressure and vacuum readings.
4. Check sheath delivery.
5. Check the baso reaction chamber
temperature.
6. Check RBC/Baso/Retic flowcell alignment.

Baso No Valley (B-NV, VB)

Definition
The Baso No Valley flag is triggered if there is no valid separation between the
mononuclear (MN) and polymorphonuclear (PMN) populations in the Baso
cytogram. The B-NV (VB) flag is not triggered if agreement between the baso
and perox results is indicated by both the following conditions:
•
WBCB and WBCP agree within specified limits (No WBC-CE flag)
•
[(%NEUT + %EOS) - %PMN] is between 0 and 7.5
Separation of the MN and PMN populations along the x axis of the Baso
cytogram is evaluated by the relative depth of the valley between the two
populations.
In normal samples, the relative Baso d/D is greater than 0.15. The B-NV flag is
triggered if the relative depth is less than 0.15 and there is no agreement between
perox and baso results.
6-28
Normal
Sample Related
•
•
•
•
•
1 MN peak
2 PMN peak
A MN/PMN Valley
Lipemia
High WBC count
Extreme eosinophilia
Malaria parasites
Aged Blood
B-NV (VB) Flag
Flags