Csf Method; Cytochemical Reactions; Measurement; Csf Scatter/Scatter Cytogram - Siemens ADVIA 2120 Operator's Manual

CSF Method

Cytochemical Reactions

The ADVIA 22120/2120i/22120/2120ii CSF Assay provides a rapid, automated
analysis of CSF samples by counting and distinguishing certain cell types. When
using the ADVIA 22120/2120i/22120/2120ii CSF Assay, the CSF sample is
mixed with ADVIA 2120/2120i CSF Reagent, which spheres and fixes the cells.
After a minimum 4-minute to 4-hour incubation period, the prepared sample is
then aspirated directly into the ADVIA 22120/2120i/22120/2120ii system.

Measurement

The ADVIA 22120/2120i/22120/2120ii CSF Assay provides a rapid, automated
analysis of CSF samples by counting and distinguishing certain cell types. When
using the ADVIA 22120/2120i/22120/2120ii CSF Assay, the CSF sample is
mixed with ADVIA 2120/2120i CSF Reagent, which spheres and fixes the cells.
After a minimum 4-minute to 4-hour incubation period, the prepared sample is
then aspirated directly into the ADVIA 22120/2120i/22120/2120ii system. The
cells are then detected and enumerated based on light scatter and absorbance
measurements using the ADVIA 22120/2120i/22120/2120ii laser optics. A
scatter versus scatter and scatter versus absorbance cytogram is displayed with
the thresholds and results automatically calculated for each sample. Reportable
parameters are WBC and RBC counts along with absolute and percentage counts
for neutrophils, lymphocytes, monocytes, polymorphonuclear (PMN), and
mononuclear (MN) cells. A research-use-only eosinophil count is also given.

CSF Scatter/Scatter Cytogram

The CSF Scatter/Scatter Cytogram is a 100 x 100-channel, two-dimensional,
pulse height distribution of the scatter high and scatter low data for WBC, RBC,
and noise, where the X-axis is high-angle scatter and the Y-axis is low angle
scatter. A fixed mask is used to set thresholds for Neutrophil, Lymphocyte,
Monocyte and RBC regions. Eosinophils fall within the Neutrophil region in this
cytogram. The number of eosinophils is determined from the Scatter/Absorption
cytogram and is subtracted from the number of cells in the Neutrophil region
prior to calculating WBC and Neutrophil parameters. At this time, eosinophil
data is for research purposes only and is non-reportable

CSF Scatter/Absorption Cytogram

The CSF Scatter/Absorption Cytogram is a 100 x 100-channel, two-dimensional,
pulse height distribution of the scatter high and absorption data for WBC, RBC,
and noise, where the X-axis is absorption and the Y-axis is high angle scatter. A
fixed mask is used to set the threshold for eosinophil analysis. Because of their
higher absorption, eosinophils fall to the right of the neutrophil population in this
cytogram and are thus able to be separated from the neutrophil population. The
number of eosinophils is subtracted from the number of cells falling in the
neutrophil region of the Scatter/Scatter cytogram before calculating WBC and
Neutrophil parameters.
8-16 Methods