Plt Volume / Pc Cytogram; Integrated Analysis - Siemens ADVIA 2120 Operator's Manual

The PLT map shows the relationship between the light-scatter measurements and
the cell-by-cell characteristics of volume and refractive index. The map grid
encompasses volumes between 0 fL and 30 fL, and refractive index between
1.3500 and 1.4000.
The platelet count includes platelets (1) and large platelets (2).
RBC fragments (4) and RBC ghosts (5) are not included in the platelet count but
are enumerated for flagging purposes.
Due to the high gain used in the platelet method, the RBCs appear in the
saturation channels at the upper right corner of the cytogram.

PLT Volume / PC Cytogram

The Platelet Volume / Refractive Index (PC) cytogram is a 100
by 100 channel display.
x axis (A): refractive index (concentration of the platelet
y axis (B): platelet volume range: 0 fL to 60 fL

Integrated Analysis

The 2-Dimensional platelet analysis (2D-PLT method) is based on the integrated
analysis of red blood cell and platelet measurements. The PLT Scatter cytogram
is formed by pairing light-scatter signals acquired at low angle - high gain and at
high angle - high gain that are converted into volume and refractive index values.
The PLT Scatter cytogram displays cells with volumes of 0 fL to 30 fL. Cell
identification and counts for cells with volumes greater than 30 fL is done using
the RBC Scatter cytogram.
Integrated analysis is used todistinguish platelets, large platelets, red blood cells,
RBC fragments, and RBC ghosts.
This example illustrates how large platelets are counted:
•
Large platelets are identified on the PLT Scatter cytogram based on their
refractive index values (1.35 to 1.40) and a volume greater than 20 fL.
8-48
component [PC]) range: 0 g/dL to 40 g/dL
Displayed cells:
1. Platelets
2. Red blood cells
Methods