Autoslide Methods; May-Grünwald Giemsa Method - Siemens ADVIA 2120 Operator's Manual

12. Replace and connect all lines disconnected in steps 4-8.
13. When the reconnection is complete turn the ADVIA 2120/2120i system back
on.
The Autoslide icon turns grey. Wait until the Autoslide established
communication with the analyzer. The icon goes from grey to orange, and
then to green. Do not initiate any command until communication is
established and the icon for the Autoslide is green.

Autoslide Methods

May-Grünwald Giemsa Method
Intended Use
The May-Grünwald Giemsa method is for in vitro diagnostic use in the
differential staining of blood, bone marrow and body fluid smears on the ADVIA
Autoslide Slide Maker Stainer. Stained smears are evaluated to identify and
quantify cells in whole blood and other specimens to aid clinicians in the
assessment of various clinical conditions.
Summary and Explanation
The reagent protocol for the ADVIA Autoslide Slide Maker Stainer consists of a
modified methylene blue-eosin stain and is based on the original stain proposed
by Pappenheim.
The ADVIA Autoslide Slide Maker Stainer is a smart, fully automated system. It
is capable of producing stained smears on all specimens run on the ADVIA
2120/2120i or only on samples with predefined criteria (i.e. flags or parameter
threshold). The autoslide module also provides a direct access port to the stainer
for pre-smeared slides.
Staining Protocol
The following is a brief description of the staining protocol:
1. Dispensing May-Grünwald Stain (Stain 1) (DN1).
2. Slide introduction.
10-20 ADVIA Autoslide Slide Maker Stainer