Calculating Rbc Parameters - Siemens ADVIA 2120 Operator's Manual

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Large platelets are also identified on the RBC Scatter cytogram (area 3
below) based on their refractive index values (1.35 to 1.40) and a volume less
than 60 fL.
The Platelet VOL histogram contains platelets and large platelets with volumes up
to 60 fL.

Calculating RBC Parameters

Reported Parameters
Parameter
RBC
MCV
RDW
CHCM
HDW
Methods
Explanation
Number of Red Cells x RBC Cal Factor x Dilution
Factor x Coincidence-correction Factor
Mean of RBC Volume histogram
100 x (SD of RBC Volume histogram ÷ MCV)
Mean of RBC HC histogram
SD of RBC HC histogram
x axis ( ) high-angle (5° to
A
15°) light scatter
y axis ( ) low-angle (2° to
B
3°) light scatter
• Area covered by the
PLT Scatter
Cytogram
• RBC fragments
• Large platelets
• RBCs
• RBC ghosts
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