Retic V / Hc Cytogram; Retic Scatter Cytogram - Siemens ADVIA 2120 Operator's Manual

1. The analyzer scans for the absorption mode between channels 2 and 25 on
the x axis.
2. The standard deviation (SD) of the six channels on either side of the mode is
calculated.
3. The RTC threshold is set 3.2 SD from the mode channel to separate the
mature RBCs from the reticulocytes.
Two additional thresholds (4)(5) are set on the x axis to separate the reticulocyte
population by their stages of maturation. The youngest reticulocytes have the
highest amounts of RNA (absorption), and appear further out on the x axis.
1. The RTC threshold channel is subtracted from 75, and the resulting
difference is divided by three.
2. The Low/Medium RTC threshold (4) is set to the RTC threshold channel
plus the value calculated in step 1.
3. The Medium/High RTC threshold (5) is set to the Low/Medium RTC
threshold channel plus the value calculated in step 1.

RETIC V / HC Cytogram

RETIC Scatter Cytogram

The RETIC Scatter cytogram is the graphic representation of two light-scatter
measurements: the high-angle, low-gain light scatter is plotted along the x axis,
and the low-angle, low-gain light scatter is plotted along the y axis. Cells
identified as mature RBCs are colored red; while cells identified as reticulocytes
are colored cyan.
Methods
On the V/HC cytogram,
hemoglobin concentration is
plotted along the x axis, and
cell volume is plotted along
the y axis.
Cells identified as mature
RBCs are red; while cells
identified as reticulocytes
are cyan.
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