Agilent Technologies 1290 Infinity User Manual page 88

4
Using the Module
Solvent Recommendation for Agilent 1290 Infinity and 1290 Infinity II and 1260 Infinity II Flexible Pumps
Handling of Acetonitrile
Acetonitrile is a solvent that is frequently used in reversed-phase
chromatography. Despite of its common use, it can be a source of issues if not
handled correctly.
As Acetonitrile ages, some residue can get stuck on internal pump surfaces
which can cause issues with valve performance and therefore affect retention
time precision.
When using acetonitrile:
• Use high-quality solvents from renowned suppliers.
• Use fresh solvents and filter them.
• Minimize exposure to light and air/oxygen.
• Choose a bottle size which fits to your application and usage.
• Acids accelerate solvent aging. If possible avoid such additives or refresh
• Pure acetonitrile ages faster. If your application allows, add about 5 % water
• Do not leave acetonitrile in unused systems to avoid aging. If not in use,
• In case of blocked valves, flush the system with hot water. Knock at valves,
Handling of Acids
Acids can corrode stainless steel and other materials in the flow path of LC
systems. For stainless steel, the minimum pH is 2.3 for corrosive acids and
pH 1 for non-corrosive acids.
Please note that for non-volatile acids like phosphoric acid or perchloric acid
concentrations increase after evaporation of water. This means that originally
diluted acids can damage parts over time, e.g. because of liquid, which has left
the solvent path through micro leaks. Such systems should be flushed
regularly with pure water and may require shorter maintenance cycles. Using
the seal wash function should be considered for protecting pump heads.
Please also refer to TechNote 01200-90090, which can be downloaded from our
website www.agilent.com.
88
solvents more frequently.
and adjust gradient compositions.
flush all solvent lines with a mixture of water and 10 % isopropanol.
flush them (see "Releasing a Stuck Inlet Valve"
ultrasonicate them, for example in methanol.
on page 169) or
1290 Infinity Quaternary Pump User Manual