Agilent Technologies 1260 Infinity Quaternary LC VL System Manual And Quick Reference
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Agilent Technologies 1260 Infinity Quaternary LC VL System Manual And Quick Reference

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Summary of Contents for Agilent Technologies 1260 Infinity Quaternary LC VL

  • Page 1 Agilent 1260 Infinity Quaternary LC VL System Manual and Quick Reference Agilent Technologies...
  • Page 2 Use, duplication or dis- closure of Software is subject to Agilent For Research Use Only Technologies’ standard commercial license terms, and non-DOD Departments and Agencies of the U.S. Government will Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...
  • Page 3 In This Book In This Book This book describes the Agilent 1260 Infinity Quaternary LC VL 1 Introduction This chapter gives an introduction to the Agilent 1260 Infinity Quaternary LC VL, the underlying concepts and the features of the Agilent 1260 Infinity Quaternary LC VL.

  • Page 4: Table Of Contents

    Thermostatted column compartment Detector 2 Specifications Physical Specifications Performance Specifications 3 Optimization of the Agilent 1260 Infinity Quaternary LC VL Optimizing the Pump Optimizing the Autosampler Optimizing the Thermostatted Column Compartment Optimizing the Detector Regarding to the System Optimizing Detection with DAD...
  • Page 5 6 Appendix Safety The Waste Electrical and Electronic Equipment Directive Lithium Batteries Information Radio Interference Sound Emission Solvent Information UV-Radiation Declaration of Conformity for HOX2 Filter Agilent Technologies on Internet Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...
  • Page 6 Contents Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...

  • Page 7: Introduction

    Thermostatted column compartment Detector Diode-Array Detector (DAD) Variable Wavelength Detector (VWD) This chapter gives an introduction to the Agilent 1260 Infinity Quaternary LC VL, the underlying concepts and the features of the Agilent 1260 Infinity Quaternary LC VL. Agilent Technologies...

  • Page 8: Introduction To The Agilent 1260 Infinity Quaternary Lc Vl

    Introduction to the Agilent 1260 Infinity Quaternary LC VL Concept of the Quaternary LC VL System The Agilent 1260 Infinity Quaternary LC VL offers the most flexibility for solvent selection and automation in HPLC method development, research and all HPLC applications requiring continuous access to a wide range of solvent choices.
  • Page 9 Infinity Series HPLC modules. • Agilent Data System helps you manage your lab for best chromatographic quality with intuitive diagnostic and monitor capabilities and alert functions to notify you of problems. Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...

  • Page 10: Features Of The Agilent 1260 Infinity Quaternary Lc Vl

    Features of the Agilent 1260 Infinity Quaternary LC VL Features of the Agilent 1260 Infinity Quaternary LC VL The Agilent 1260 Infinity Quaternary LC VL is designed to offer the greatest flexibility for performing analytical liquid chromatography using all types of current and emergent column technologies.

  • Page 11: System Components

    Signals triggered by UV absorption are sampled to be converted to electrical signals in order for display and software handling. The Agilent 1260 Infinity Quaternary LC VL are described in more detail in the following sections. All modules are stackable, see “One Stack...

  • Page 12: Optimizing The Stack Configuration

    Infinity LC System in the following configuration (see Figure 1 on page 13 and Figure 2 on page 14). This configuration optimizes the flow path for minimum delay volume and minimizes the bench space required. Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...
  • Page 13 Introduction Optimizing the Stack Configuration Figure 1 Recommended Stack Configuration (Front View) Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...
  • Page 14 Introduction Optimizing the Stack Configuration Figure 2 Recommended Stack Configuration (Rear View) Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...
  • Page 15 A slightly longer capillary is required between the pump and autosampler. See Figure 3 on page 15 and Figure 4 on page 16. Figure 3 Two stack configuration (front view) Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...
  • Page 16 Introduction Optimizing the Stack Configuration Figure 4 Two stack configuration (rear view) Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...

  • Page 17: Quaternary Pump

    A purge valve including a PTFE frit is fitted at the pump outlet for convenient priming of the pump head. An active seal wash (optional) is available for applications using concentrated buffers as solvents. Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...
  • Page 18 Introduction Quaternary pump Hydraulic Path Figure 5 Hydraulic Path of the Quaternary Pump Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...

  • Page 19: Autosampler

    G4208 A Instant Pilot or from the Agilent Data System. Three sample-rack sizes are available. The standard full-size rack holds 100 × 1.8 mL vials, while the two half-size racks provide space for 40 × 1.8 mL Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...
  • Page 20 Control of the vial temperature in the thermostatted autosampler is achieved using the additional Agilent 1260 Infinity ALS thermostat. Details of this module are given in the Agilent 1260 Infinity Autosampler Thermostat manual. Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...
  • Page 21 9 The gripper arm checks if the safety flap is in position. 10 The gripper arm replaces the vial, and returns to the home position. Simultaneously, the needle lowers into the seat. 11 The injection valve switches to the mainpass position. Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...
  • Page 22 (Figure 8 on page 22). Solvent from the pump enters the valve unit at port 1, and flows directly to the column through port 6. Figure 8 Bypass Position Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...
  • Page 23 The needle is lowered into the needle seat, and the injection valve switches back to the mainpass position, flushing the sample onto the column (Figure 10 on page 23). Figure 10 Mainpass Position (Sample Injection) Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...

  • Page 24: Thermostatted Column Compartment

    ® • Optional high-quality Rheodyne column switching valves with ceramic stator-face assemblies for prolonged lifetime. For specifications, see “Performance Specifications (G1316A)” on page 41. Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...

  • Page 25: Detector

    • Additional diagnostic signals for temperature and lamp voltage monitoring. • Easy exchange of flow cell by cartridge design. For specifications, see “Performance Specifications (G4212B)” on page 43. Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...
  • Page 26 [4] through the slit assembly [5] onto a holographic grating [6] light being dispersed onto the diode array [7]. This allows simultaneous access to all wavelength information. Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...
  • Page 27 The light source for the UV-wavelength range is a long-life UV-lamp with RFID tag. As a result of plasma discharge in low-pressure deuterium gas, the lamp emits light over the 190 nm to approximately 800 nm wavelength range. Figure 12 UV-Lamp Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...
  • Page 28 The fixed slit combines the required optical functions - slit and shutter - in a simple and compact component. The slit width is directly controlled by the micro-processor of the instrument and is fixed to 4 nm. Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...
  • Page 29 The diode array is a series of 1024 individual photodiodes and control circuits located on a ceramic carrier. It has a wavelength range from 190 – 640 nm and the sampling interval is ∼0.5 nm. Figure 14 Grating and diode array Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...
  • Page 30 This detectors cannot be operated with a G1323B Control Module. Use the Instant Pilot N O T E (G4208A) as local controller. For specifications refer to “Performance Specifications (G1314F)” on page 44. Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...
  • Page 31 The cutoff filter is moved into the lightpath above 370 nm to reduce higher order light. Figure 15 Optical Path of the Variable Wavelength Detector Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...
  • Page 32 0.12 0.17 Inlet length Outlet i.d. 0.25 0.25 0.17 0.25 Outlet length Materials in contact SST, quartz, PTFE, SST, quartz, PTFE SST, quartz, PTFE SST, quartz, Kapton with solvent PEEK Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...
  • Page 33 The entrance slit assembly has an exchangeable slit. The standard one has a 1-mm slit. For replacement and calibration purposes to optimize the alignment, a slit with a hole is needed. Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...
  • Page 34 A photo sensor determines the correct position. Mirror Assemblies M1 and M2 The instrument contains two spherical mirrors (M1 and M2). The beam adjustable is vertically and horizontally. Both mirrors are identical. Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...
  • Page 35 The photo diode current is directly converted to digital data direct photo current digitalization. The data is transferred to the detector main board . The photo diode ADC boards are located close to the photo diodes. Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...
  • Page 36 Introduction Detector Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...

  • Page 37: Specifications

    Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference Specifications Physical Specifications Performance Specifications Specification Conditions This chapter provides information about specifications for the LC system. Agilent Technologies...

  • Page 38: Physical Specifications

    For indoor use only. IEC, CSA, UL, EN This temperature range represents the technical specifications for this instrument. The mentioned temperatures may not be suitable for all applications and all types of solvents. Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...
  • Page 39 Using the autosampler at high environmental temperatures may cause the rear panel to become hot. ➔ Do not use the autosampler at environmental temperatures higher than 50 °C (122 °F) Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...

  • Page 40: Performance Specifications

    Control and data evaluation Agilent control software Analog output For pressure monitoring, 2 mV/bar, one output Communications Controller-area network (CAN), RS-232C, APG Remote: ready, start, stop and shut-down signals, LAN optional Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...
  • Page 41 Pilot and Agilent data system), leak detection, safe leak handling, leak output signal for shutdown of pumping system. Low voltages in major maintenance areas. GLP features Column-identification module for GLP documentation of column type. Housing All materials recyclable Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...
  • Page 42 40 × 2 mL vials in ½ tray 15 × 6 mL vials in ½ tray (Agilent vials only) Injection cycle time 50 s for draw speed 200 µL/min, ejection speed 200 µL/min, injection volume 5 µL Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...
  • Page 43 3.3.2 SP2 or above MassHunter B.04.00 and B.03.01 SP2 or above Local Control Agilent Instant Pilot (G4208A) B.02.11 or above Test and diagnostic Agilent LabAdvisor B.01.03 SP4 or above software Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...
  • Page 44 The UV-lamp is equipped with RFID tag that holds lamp typical information. Short term noise Under specified condtions. See “Specification ± 0.25·10 AU at 230 nm (G1314F) Conditions (VWD)” on page 48 below the table. Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...
  • Page 45 Time programmable Wavelength, Reference and Sample scan, balance, steps,lamp on/off Spectral tools Stop-flow wavelength scan Analog outputs Recorder/integrator: 100 mV or 1 V, output range 0.001 – 2 AU, one output Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...
  • Page 46 Verification of wavelength accuracy with built-in holmium oxide filter. RFID for electronics records of flow cell and UV lamp conditions (path length, volume, product number, serial number, test passed, usage) Housing All materials recyclable. Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...
  • Page 47 Performance tests should be done with a completely warmed up optical unit (> two hours). ASTM measurements require that the detector should be turned on at least 24 h before start of testing. Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...
  • Page 48 Time Constant versus Response Time According to ASTM E1657-98 „Standard Practice of Testing Variable-Wavelength Photometric Detectors Used in Liquid Chromatography” the time constant is converted to response time by multiplying by the factor 2.2. Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...

  • Page 49: Optimization Of The Agilent 1260 Infinity Quaternary Lc Vl

    Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference Optimization of the Agilent 1260 Infinity Quaternary LC VL Optimizing the Pump Operational Hints for the Vacuum Degasser Operational Hints for the Multi Channel Gradient Valve (MCGV) When to Use the Seal Wash Option...

  • Page 50: Optimizing The Pump

    Gravity forces the salt particles to fall down. Normally the A channel of the valve is used for the aqueous/salt solution and the B channel of the pump is used for the organic solvent. If used Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...

  • Page 51: When To Use The Seal Wash Option

    (90 %) and isopropanol (10 %) as wash solvent. This mixture prevents growth of algae or bacteria in the wash bottle and reduces the surface tension of the water. Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...

  • Page 52: Choosing The Right Pump Seals

    1 Start the pump with the required flow rate. 2 Before starting the optimization procedure, the flow must be stable. Check the tightness of the system with the pressure test. Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...
  • Page 53 Table 9 Solvent Compressibility Solvent (pure) Compressibility (10-6/bar) Acetone Acetonitrile Benzene Carbon tetrachloride Chloroform Cyclohexane Ethanol Ethyl acetate Heptane Hexane Isobutanol Isopropanol Methanol 1-Propanol Toluene Water Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...

  • Page 54: Optimizing The Autosampler

    When the automated needle wash is used, the needle is moved into a wash vial after the sample is drawn. By washing the needle after drawing a sample, the sample is removed from the surface of the needle immediately. Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...
  • Page 55 Otherwise the separation could be influenced, especially if microbore columns are used. Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...
  • Page 56 If this is not known use 2 or 3 solvents of different polarity. Use several milliliters to clean the seat. • Clean the needle seat with a tissue and remove all liquid from it. • RESET the injector. Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...

  • Page 57: Fast Injection Cycle And Low Delay Volume

    Especially at low flow rates this can lead to retention time changes which are too big for the actual calibration table. To overcome this it is recommended to add an additional “blank” injection as last injection to the sequence. Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...

  • Page 58: Precise Injection Volume

    The default draw speed is 200 µL/min. This speed is suitable for the majority of applications, however, when using viscous samples, set the draw speed to lower speed for optimum results. A DRAW statement in an injector Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...
  • Page 59 A faster eject speed shortens the time required to run the injector program. When using viscous samples, a high eject speed should be avoided. Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...

  • Page 60: Choice Of Rotor Seal

    1 and 14. Strong oxidizing acids such as concentrated nitric and sulfuric acids are not compatible N O T E with PEEK. Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...

  • Page 61: Optimizing The Thermostatted Column Compartment

    • Keep the left and right heat exchanger temperature the same unless you do specific applications. • Assure that the front cover is always closed. Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...

  • Page 62: Optimizing The Detector Regarding To The System

    Agilent 1290 Infinity/Agilent 1260 Infinity LC System due to the narrow-bore (0.12 mm i.d.) tubing, the low-volume heat exchangers in the column compartment and the Max-Light cartridge cell in the detector. Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...

  • Page 63: Optimizing Detection With Dad

    The information below will guide you on how to get the best detector performance. Follow these rules as a start for new applications. It gives rules-of-thumb for optimizing detector parameters. Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...

  • Page 64: Optimization Overview

    Select the reference wavelength with broad bandwidth (30...100 nm) • baseline drift due to RI effects. wavelength range where your analytes have little or no absorbance (e.g. sample at 254 nm, reference at 320 nm). Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...

  • Page 65: Optimizing For Sensitivity, Selectivity, Linearity And Dispersion

    1 In Absorbance divide by the path length AU/cm, that is then similar to [ε x C]. Advantage: samples with same concentration have same peak height also at cells with different path lengths. Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...
  • Page 66 When set correctly, such filters reduce baseline noise significantly ( Figure 18 on page 67), but reduce peak height only slightly. In addition, these filters reduce the data rate to allow Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...
  • Page 67 (over-filtering) will reduce peak height by about 20 % and reduce baseline noise by a factor of 1.4. This gives you the best possible signal-to-noise ratio, but may affect peak resolution. Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...
  • Page 68 This provides full information but fills up your disk space rather quickly. Spectra can be used to check a peak’s purity and identity. Spectral information is also useful to optimize wavelength settings for your chromatographic signal. Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...
  • Page 69 If you work with high concentrations, you may get better linearity above 1.5 AU by setting the sample wavelength to a valley in the spectrum, like 225 nm for anisic acid. Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...
  • Page 70 4 nm wide band. The signal-to-noise ratio for a 30 nm bandwidth is twice that for a 4 nm bandwidth in our example. Figure 20 Influence of Bandwidth on Signal and Noise Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...
  • Page 71 With this technique, PTH-amino acids can be quantified in the low picomole range even in a gradient analysis. Figure 21 Gradient Analysis of PTH-Amino Acids (1 pmol each), with and without Reference Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...
  • Page 72 Only for such cases, increase the margin for negative absorbance to avoid overflow of the analog-to-digital converter. Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...

  • Page 73: Optimizing Selectivity

    Figure 22 Wavelength Selection for Peak Suppression With a UV-visible detector based on a diode array and the correct choice of a reference wavelength setting, quantitative detection is possible. To suppress Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...
  • Page 74 An example is shown in Figure 24 on page 75 and Figure 25 on page 75. Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...
  • Page 75 249 and 224 nm was within this range, is the signal plotted. Of all four peaks, only the third fulfilled the criterion (Figure 25 on page 75). The others were not plotted. Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...

  • Page 76: Warm Up Of The Detector

    The example below was done under stable environmental conditions. The figures below show the first two hours of a detector warm-up phase. The lamp was turned on immediately after turn on of the detector. Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...
  • Page 77 Optimization of the Agilent 1260 Infinity Quaternary LC VL Optimizing Detection with DAD Figure 26 Detector Warm-up – 1 hour Figure 27 Detector Warm-up – 2 hour Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...

  • Page 78: Optimizing Detection With Vwd

    Follow these rules as a start for new applications. It gives a rule-of-thumb for optimizing the detector parameters. Match the Flow Cell to the Column Standard HPLC Applications Figure 28 Choosing a Flow Cell (Standard HPLC Applications) Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...
  • Page 79 To determine the volume of your peaks, take the peak width as reported in the integration results multiply it by the flow rate and divide it by 3). Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...
  • Page 80 6/6.10 Micro flow cell 3 mm, 2 µL (p/n G1314-60187) 2.80 ± 0.19 mm 3/2.8 High pressure flow cell 10 mm, 14 µL (p/n G1314-60182) 10.00 ± 0.19 mm 6/5.75 Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...

  • Page 81: Set The Detector Parameters (Vwd)

    • preferably where the spectrum is flat for better linearity. 3 Consider to use time-programming to further optimization. Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...
  • Page 82 Optimization of the Agilent 1260 Infinity Quaternary LC VL Optimizing Detection with VWD Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...

  • Page 83: System Setup And Installation

    Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference System Setup and Installation Installing Software Installing the Modules Priming the System Integration Into the Network This chapter includes information on software installation, stack configurations and preparing the system for operation.

  • Page 84: Installing Software

    • Attempts to identify supported LAN-based instruments that are powered on and connected to your PC or lab’s network, • Automatically suggests replacements parts and troubleshooting tasks for some common instrument problems. Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...

  • Page 85: Installing The Modules

    You can ensure optimum performance by installing the system in one stack and two stack configurations ( “One Stack Configuration” on page 12 and “Two Stack Configuration” on page 15). These configurations optimize the system flow path, ensuring minimum delay volume. Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...
  • Page 86 Pump for approximately 10 minutes at your desired flow rate before starting an analysis. This will allow the vacuum degasser to properly degas the solvent in the degasser tubes. Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...
  • Page 87 6 Pump 30 mL isopropanol to remove residual air bubbles. 7 Switch to the next solvent channel and repeat steps 5 and 6 until all channels have been purged. 8 Turn the flow off and close the purge valve. Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...
  • Page 88 3 Repeat step 1 and 2 for the other channel(s) of the pump. 4 Set the required composition and flow rate for your application and close the purge valve. 5 Pump for approximately 10 minutes before starting your application. Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...
  • Page 89 8 Repeat steps 1 to 7 for the other channel(s) of the pump. 9 Install the desired column, set the required composition and flow rate for your application and equilibrate the system for approx. 10 minutes prior to starting a run. Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...
  • Page 90 Hexane + 5% isopropanol Good wetting properties phase seals (P/N 0905-1420) Integration Into the Network For network integration of your system refer to user manuals of your modules (chapter LAN Configuration). Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...

  • Page 91: Quick Start Guide

    Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference Quick Start Guide Preparing the System Turning the System ON Loading the Default Method Configuring the Online Plot Purging the Pump Setting Up the Method This chapter provides information on data acquisition and data analysis with the Agilent 1260 Infinity Quaternary LC VL.

  • Page 92: Preparing The System

    System On/Off button in the bottom right of the system diagram. The system status changes from Not Ready (yellow indication) to Ready (green indication) after a short delay as the setpoints are attained. Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...

  • Page 93: Loading The Default Method

    Note that this method can never be overwritten with new parameters. Hence clicking on N O T E Save will re-direct you into the Save As... function, so that you must enter a different method name. Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...

  • Page 94: Configuring The Online Plot

    2 To configure the desired signal(s) in the Online Plot window, click Change…. The Edit Signal Plot setup page opens. 3 In the Available Signals box, highlight the required signal(s), and click Add to move them to the Selected Signals box. Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...
  • Page 95 The Balance button zeroes all detectors when pressed. Changes made in the Online Plot page do not in any way affect the data stored into the N O T E individual data files. Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...

  • Page 96: Purging The Pump

    (isopropanol is often a good choice, check with a solvent miscibility table). For details on the purging procedure, refer to “Priming the System” page 86. Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...

  • Page 97: Setting Up The Method

    Injection with Needle Wash • Mode Flush Port, Time: 6 s b Other parameters can remain at default settings. Click OK to exit the window. The changes are sent to the autosampler module. Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...
  • Page 98 7 Allow the system to equilibrate for at least 10 min, and check that the baseline in the Online Plot is stable before starting the analysis. Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...
  • Page 99 Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference Appendix Safety The Waste Electrical and Electronic Equipment Directive Lithium Batteries Information Radio Interference Sound Emission Solvent Information UV-Radiation Declaration of Conformity for HOX2 Filter Agilent Technologies on Internet This chapter provides addition information on safety, legal, web and the Edit Entire Method.

  • Page 100: Appendix Safety

    ➔ Do not proceed beyond a caution until you have fully understood and met the indicated conditions. Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...
  • Page 101 Make sure that only fuses with the required rated current and of the specified type (normal blow, time delay, and so on) are used for replacement. The use of repaired fuses and the short-circuiting of fuse holders must be avoided. Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...
  • Page 102 When working with solvents please observe appropriate safety procedures (e.g. goggles, safety gloves and protective clothing) as described in the material handling and safety data sheet by the solvent vendor, especially when toxic or hazardous solvents are used. Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...

  • Page 103: The Waste Electrical And Electronic Equipment Directive

    Monitoring and Control Instrumentation product. Do not dispose off in domestic household waste N O T E To return unwanted products, contact your local Agilent office, or see www.agilent.com more information. Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...

  • Page 104: Lithium Batteries Information

    Ved udskiftning benyttes kun batteri som anbefalt av apparatfabrikanten. ➔ Brukt batteri returneres appararleverandoren. Bij dit apparaat zijn batterijen geleverd. Wanneer deze leeg zijn, moet u ze niet weggooien N O T E maar inleveren als KCA. Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...

  • Page 105: Radio Interference

    If test and measurement equipment is operated with unscreened cables, or used for measurements on open set-ups, the user has to assure that under operating conditions the radio interference limits are still met within the premises. Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...

  • Page 106: Sound Emission

    This product has a sound pressure emission (at the operator position) < 70 dB. • Sound Pressure Lp < 70 dB (A) • At Operator Position • Normal Operation • According to ISO 7779:1988/EN 27779/1991 (Type Test) Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...

  • Page 107: Solvent Information

    THF, dioxane, di-isopropylether) such ethers should be filtered through dry aluminium oxide which adsorbs the peroxides, • Solvents containing strong complexing agents (e.g. EDTA), • Mixtures of carbon tetrachloride with 2-propanol or THF. Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...

  • Page 108: Uv-Radiation

    Typically the radiation values are much smaller than these limits: Table 16 UV-Radiation Typical Values Position Effective Irradiance Lamp installed, 50-cm distance average 0.016 µW/cm Lamp installed, 50-cm distance maximum 0.14 µW/cm Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...

  • Page 109: Declaration Of Conformity For Hox2 Filter

    Appendix Declaration of Conformity for HOX2 Filter Declaration of Conformity for HOX2 Filter Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...

  • Page 110: Agilent Technologies On Internet

    For the latest information on products and services visit our worldwide web site on the Internet at: http://www.agilent.com Select Products/Chemical Analysis It will provide also the latest firmware of the modules for download. Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...
  • Page 111 Beer-Lambert (law) dimension Beer-Lambert diode GLP features absorbance width gradient formation boards draw speed grating photodiode boards (ADC) DRAW assembly bypass drift (ASTM) and noise drift Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...
  • Page 112 Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...
  • Page 113 WEEE directive standards lithium batteries weight stepper motor safety suppression general information quantifying symbols system setup and installation sample and reference wavelength network Integration sampling rate data rate Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference...
  • Page 114 Agilent 1260 Infinity Quaternary LC VL. The manual describes the following: • introduction, • product description, • system optimization, • setup and installation, • quick start guide. © Agilent Technologies 2010 Printed in Germany 06/2010 *G1311-90310* *G1311-90310* G1311-90310 Agilent Technologies...

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