tecan ls series Operating Manual page 81

2004-11 Operating Manual for the LS Series Scanner No. 30001798 Rev. No. 1.5
Increasing the angle of incidence might reduce the background in your images.
However, if it is too high for your kind of sample only a part of your sample area
could visible on the scanned image. If the autofocus angle is set too high
autofocus might fail. Use simple geometry according to figure below to calculate
the appropriate angles for your samples.
25°
Figure 6-9
Adjustable laser beam angle (0.1° increments)
High working distance (6.5 mm)
(absolut range of object height)
Figure 6-10
If your microplates have a transparent bottom and your process allows
them to be measured upside down it is still a good idea to compare an
upside down measurement with default settings with an upright
measurement with the angles set to MTP mode or another value
determined as optimum for your samples.
6. LSServer, Scanner Shell and Download
6.5
5-16 mm
(Set beam angle to 0° to
avoid shadows in wells)
Note
0°
6.5 mm
max object
depth
6-51