tecan ls series Operating Manual page 76

6. LSServer, Scanner Shell and Download
Focus Gain
Focus Gain
controls
Focal Plane
Focal Plane
group box
6-46 Operating Manual for the LS Series Scanner No. 30001798 Rev. No. 1.5
Focus Gain controls
Use the Focus Gain controls to set the PMT gain for autofocus
measurement.
The default value for the focus gain is 140. The gain is controlled
automatically and dependent on the focus intensity profiles, but can also
be adjusted manually either by using the arrows or typing in a value.
The intensity profile from autofocus scanning in z-direction shows the
different surfaces of slides (different planes). The first surface found is
defined as default focal plane, but any of three can be selected.
Plane 1
Plane 2
Plane 3
•
Plane 1 option button:
Select Plane 1 option button to select Plane 1 (signal intensity) as focal
plane calculated in the order from top to bottom from the Focus.tif images.
The measurement will be performed on the selected plane 1.
•
Plane 2 option button:
Select Plane 2 option button to select Plane 2 (signal intensity) as focal
plane calculated in the order from top to bottom from the Focus.tif images.
The measurement is performed on the selected second plane from the
signal intensity profile in z-direction.
•
Plane 3 option button:
Select Plane 3 option button to select Plane 3 (signal intensity) as focal
plane calculated in the order from top to bottom from the Focus.tif
images. The measurement is performed on the selected third plane of the
signal profile in z.
2004-11