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6. LSServer, Scanner Shell and Download
6-80
Operating Manual for the LS Series Scanner No. 30001798 Rev. No. 1.5
2. Channel 2 tab
Before calculating crosstalk correction a so-called K-factor must be
determined. This is done during a measurement without taking into account
crosstalk correction. Adjusted values in this measurement session must be
entered in Channel 2 tab.
The Channel 2 tab contains the following elements:
Dye 2
In the Dye 2 edit box the description of used dye must be
edit box
entered.
K2
In the K2 edit box the correction factor for contribution of dye
edit box
with longer emission wavelength into the detection channel
of dye with shorter emission wavelength must be entered.
The K2 default value is set to 0. This means no crosstalk
correction will be done, no other parameters (Dye, gain)
must be specified.
In the Gain 1 edit box PMT Gain channel 1 must be entered.
Gain 1
edit box
Gain 2
In the Gain 2 edit box PMT Gain channel 2 must be entered.
edit box
Next part of Crosstalk dialog box contains the following elements:
In Channel 1 Laser combo box all lasers found in current
Channel 1 Laser
combo box
connected instrument are displayed. The one used for
crosstalk correction must be selected.
Channel 2 Laser
In Channel 2 Laser combo box all lasers found in current
combo box
connected instrument are displayed. The one used for
crosstalk correction must be selected.
In Channel 1 Filter combo box all filters found in filter slide,
Channel 1 Filter
combo box
loaded in slot 1 of current connected instrument, are
displayed. The one used for crosstalk correction must be
selected.
Channel 2 Filter
In Channel 2 Filter combo box all filters found in filter slide,
combo box
loaded in slot 2 of current connected instrument, are
displayed. The one used for crosstalk correction must be
selected.
Date static box
The Date static box shows the current date.
Format = DD.MM.YY
Name edit box
In the Name edit box the user can type in his name or initials
or some other comment.
2004-11
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