tecan ls series Operating Manual page 71

Crosstalk
Crosstalk
group box
2004-11 Operating Manual for the LS Series Scanner No. 30001798 Rev. No. 1.5
The Crosstalk group box contains the following elements:
•
The Crosstalk correction check box
Select Crosstalk correction to select a correction factor
(= K-factor) from the Crosstalk list box.
Dual channel instruments, operated in Dual Parallel Scan Mode, are
corrected for the spectral crosstalk from the first dye into the second
detection channel by applying K-factors.
The Crosstalk correction check box is automatically available, if a Gain
Calibration file and a Crosstalk file are found for the current laser and filter
combination (selected in the Laser Channel 1 / Channel 2 and Filter
Channel 1 / Channel 2 combo box).
Otherwise Crosstalk elements are not enabled and no crosstalk correction
factor can be set.
The defined K-factors for the current laser / filter combination are displayed
in the Crosstalk list box. Clicking on a Crosstalk list item sets the K-factor
(values appearing in K1 / K2 static text box), which will be used when
starting the next measurement, if the Crosstalk correction check box has
been selected. If the Crosstalk correction check box has not been selected
no correction is done.
•
The K1 static text box displays the current selected crosstalk factor K1
(residual signal contribution from the first dye detected in the second
channel will be corrected).
•
The K2 static text box displays the current selected crosstalk factor K2
(residual signal contribution from the second dye detected in the first
channel will be corrected).
•
In the Crosstalk list box all crosstalk factor records, corresponding to
selected laser / filter combination, are displayed:
Format K1 = Dye 1, Name, Date
Format K2 = Dye 2, Name , Date
For further information refer to the chapter 6.4.6 Service Menu -
Crosstalk...in this manual.
In order to obtain reliable results it is strongly recommended to place
reference spots with one dye only onto your sample. These spots should
represent every dye of your experiment and should be used for QC
6. LSServer, Scanner Shell and Download
Note
purposes.
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