tecan ls series Operating Manual page 54

6. LSServer, Scanner Shell and Download
Focus Prescan
6-24 Operating Manual for the LS Series Scanner No. 30001798 Rev. No. 1.5
In the Measurement Control dialog box, click Prescan and select Focus
Prescan to perform a focus scan of the first selected scan area of the currently
used MTP adapter.
The focus prescan enables the user to define the desired focal plane and the
focus offset on a z-scan image. This can also be used for probes, where the auto
focus fails. The focus offset can be adjusted directly on the image, so refraction
considerations are not necessary.
If no focus was previously selected, the focus prescan will be aborted with
following error message: 'No focus mode selected. Please select focus mode in
Advanced Autofocus dialog!'
Fluorescence focus scans will be performed with the laser / filter setting of
channel 1. This focus scan mode can be relevant for gel applications. The default
settings are reflection modus with the red ( 633nm ) laser.
Click OK, and Focus Prescan dialog box appears:
Example of HS and well mode auto focus.
The Focus Prescan dialog box has the following buttons:
Reset Click Reset to return all values to the default settings.
button
OK button Click OK to save the settings. The focus values are stored for the
currently scanned slide. A message appears, which asks if the focus
offset should only be stored for this slide or for all slides of the
adapter.
Cancel Click Cancel to close the dialog box.
button The selected options are not saved.
Help Click Help to display the online help for the dialog box.
button
2004-11