tecan ls series Operating Manual page 72

6. LSServer, Scanner Shell and Download
Integration time
Integration
time group box
Sensitivity
Sensitivity
group box
6-42 Operating Manual for the LS Series Scanner No. 30001798 Rev. No. 1.5
Select the Optimize integration time (OIT) check box to increase the
sensitivity of the scan due to longer integration of each pixel. If the
integration time is longer more photons (i.e. more signal) are gathered for
each pixel resulting in higher intensity and better signal to noise ratio.
OIT not selected:
•
Same Integration time for each scanning amplitude
•
Comparable signal values for all scanning amplitudes in the same
resolution
OIT selected:
•
Increase sensitivity of scan due to longer integration time of each pixel.
•
Longer integration time lead to more photons, which can gathered for
each pixel.
•
Higher signal to noise ratio = higher sensitivity
•
Not comparable signal values between scans with different amplitudes.
•
Comparable range of amplitudes:
22 mm
20 mm
15 mm
10 mm
5 mm
Oversampling factor edit box.
Enter a certain Oversampling factor to average scan lines and to combine
them into one pixel.
Oversampling factor can be a value between 1-10.
For 4 µm scan resolution: n (max) = 3
For 6 µm scan resolution: n (max) = 4
For 10 µm scan resolution: n (max) = 8
For 20 µm scan resolution: n (max) = 10
For 40 µm scan resolution: n (max) = 10
For example, with an oversampling factor of three, one pixel is scanned
three times and the average of these values is calculated. Oversampling
therefore reduces noise but increases scan time by the factor. The average
will be calculated during scanning.
In theory, the signal to noise ratio is increased by n
(n= oversampling factor). For example, a factor of 4 will increase the signal
to noise ratio by a factor of 2.
2004-11